{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T11:31:39Z","timestamp":1769340699830,"version":"3.49.0"},"reference-count":119,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006595","name":"UEFISCDI Romania","doi-asserted-by":"publisher","award":["PN-III-P1-1.1-PD-2019-0774"],"award-info":[{"award-number":["PN-III-P1-1.1-PD-2019-0774"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006595","name":"UEFISCDI Romania","doi-asserted-by":"publisher","award":["1170\/3\/14.01.2021"],"award-info":[{"award-number":["1170\/3\/14.01.2021"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]},{"name":"\u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy","award":["PN-III-P1-1.1-PD-2019-0774"],"award-info":[{"award-number":["PN-III-P1-1.1-PD-2019-0774"]}]},{"name":"\u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy","award":["1170\/3\/14.01.2021"],"award-info":[{"award-number":["1170\/3\/14.01.2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>One of the objectives of this study consists of the assessment of the antitumor activity of several extracts from three selected plant species: Xanthium spinosum L., Trifolium pratense L., and Coffea arabica L. and also a comparative study of this biological activity, with the aim of establishing a superior herbal extract for antitumor benefits. The phytochemical profile of the extracts was established by HPLC-MS analysis. Further, the selected extracts were screened in vitro for their antitumor activity and antioxidant potential on two cancer cell lines: A549\u2014human lung adenocarcinoma and T47D-KBluc\u2014human breast carcinoma and on normal cells. One extract per plant was selected for in vivo assessment of antitumor activity in an Ehrlich ascites mouse model. The extracts presented high content of antitumor compounds such as caffeoylquinic acids in the case of X. spinosum L. (7.22 \u00b5g\/mL\u2014xanthatin, 4.611 \u00b5g\/mL\u20144-O-caffeoylquinic acid) and green coffee beans (10.008 \u00b5g\/mL\u2014cafestol, 265.507 \u00b5g\/mL\u20144-O-caffeoylquinic acid), as well as isoflavones in the case of T. pratense L. (6806.60 ng\/mL\u2014ononin, 102.78 \u00b5g\/mL\u2014biochanin A). Concerning the in vitro results, the X. spinosum L. extracts presented the strongest anticancerous and antioxidant effects. In vivo, ascites cell viability decreased after T. pratense L. and green coffee bean extracts administration, whereas the oxidative stress reduction potential was important in tumor samples after T. pratense L. Cell viability was also decreased after administration of cyclophosphamide associated with X. spinosum L. and T. pratense L. extracts, respectively. These results suggested that T. pratense L. or X. spinosum L. extracts in combination with chemotherapy can induce lipid peroxidation in tumor cells and decrease the tumor viability especially, T. pratense L. extract.<\/jats:p>","DOI":"10.3390\/plants12091840","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T12:08:31Z","timestamp":1682942911000},"page":"1840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["An In Vitro and In Vivo Assessment of Antitumor Activity of Extracts Derived from Three Well-Known Plant Species"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6672-1535","authenticated-orcid":false,"given":"Octavia","family":"Gligor","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0046-0803","authenticated-orcid":false,"given":"Simona","family":"Clichici","sequence":"additional","affiliation":[{"name":"Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remus","family":"Moldovan","sequence":"additional","affiliation":[{"name":"Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoleta","family":"Decea","sequence":"additional","affiliation":[{"name":"Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana-Maria","family":"Vlase","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ionel","family":"Fize\u0219an","sequence":"additional","affiliation":[{"name":"Department of Toxicology, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5375-6053","authenticated-orcid":false,"given":"Anca","family":"Pop","sequence":"additional","affiliation":[{"name":"Department of Toxicology, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piroska","family":"Virag","sequence":"additional","affiliation":[{"name":"Department of Radiobiology and Tumor Biology, Oncology Institute \u201cProf. Dr. Ion Chiricu\u021b\u0103\u201d, 34-36 Republicii Street, 400015 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7447-4925","authenticated-orcid":false,"given":"Gabriela Adriana","family":"Filip","sequence":"additional","affiliation":[{"name":"Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0664-3387","authenticated-orcid":false,"given":"Laurian","family":"Vlase","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology and Biopharmaceutics, University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianina","family":"Cri\u0219an","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1158\/2159-8290.CD-21-1059","article-title":"Hallmarks of cancer: New dimensions","volume":"12","author":"Hanahan","year":"2022","journal-title":"Cancer Discov."},{"key":"ref_2","first-page":"451","article-title":"Global Cancer Statistics 2022: The trends projection analysis","volume":"10","author":"Chhikara","year":"2023","journal-title":"Chem. 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