{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:44:23Z","timestamp":1790631863322,"version":"4.1.0"},"reference-count":81,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>In this study, the leaves of Kalanchoe fedtschenkoi were consecutively macerated with hexane, chloroform, and methanol. These extracts were used to assess the bioactivities of the plant. The antimicrobial activity was tested against a panel of Gram-positive and -negative pathogenic bacterial and fungal strains using the microdilution method. The cytotoxicity of K. fedtschenkoi extracts was investigated using human-derived macrophage THP-1 cells through the MTT assay. Finally, the anti-inflammatory activity of extracts was studied using the same cell line by measuring the secretion of IL-10 and IL-6. The phytoconstituents of hexane and chloroform extracts were evaluated using gas chromatography\u2013mass spectrometry (GC\/MS). In addition, high-performance liquid chromatography (HPLC) was used to study the phytochemical content of methanol extract. The total flavonoid content (TFC) of methanol extract is also reported. The chemical composition of K. fedtschenkoi extracts was evaluated using Fourier-transform infrared spectroscopy (FTIR). Results revealed that the chloroform extract inhibited the growth of Pseudomonas aeruginosa at 150 \u03bcg\/mL. At the same concentration, methanol extract inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Regarding their cytotoxicity, the three extracts were highly cytotoxic against the tested cell line at IC50 &lt; 3 \u03bcg\/mL. In addition, the chloroform extract significantly stimulated the secretion of IL-10 at 50 \u03bcg\/mL (p &lt; 0.01). GC\/MS analyses revealed that hexane and chloroform extracts contain fatty acids, sterols, vitamin E, and triterpenes. The HPLC analysis demonstrated that methanol extract was constituted by quercetin and kaempferol derivatives. This is the first report in which the bioactivities and chemical profiles of K. fedtschenkoi are assessed for non-polar and polar extracts.<\/jats:p>","DOI":"10.3390\/plants12101943","type":"journal-article","created":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T03:49:06Z","timestamp":1683690546000},"page":"1943","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8252-5211","authenticated-orcid":false,"given":"Jorge L.","family":"Mej\u00eda-M\u00e9ndez","sequence":"first","affiliation":[{"name":"Laboratory in Phytochemistry Research, Chemical Biological Sciences Department, Universidad de las Am\u00e9ricas Puebla, Ex Hacienda Sta. Catarina M\u00e1rtir S\/N, San Andres Cholula 72810, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7740-9299","authenticated-orcid":false,"given":"Horacio","family":"Bach","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Faculty of Medicine, University of British Columbia, Vancouver, BC V6G 3Z6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Lorenzo-Leal","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Faculty of Medicine, University of British Columbia, Vancouver, BC V6G 3Z6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6810-8032","authenticated-orcid":false,"given":"Diego E.","family":"Navarro-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Tecnologico de Monterrey, Escuela de Ingenier\u00eda y Ciencias, Campus Guadalajara, Av. Gral. 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