{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:44:15Z","timestamp":1790700255510,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T00:00:00Z","timestamp":1684195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Berberis libanotica Ehrenb. ex C.K. Schneider of the Berberidaceae family is an endemic Lebanese plant and is widely used in folk medicine. This study highlights the phytochemical composition and biological activities (in vitro) of fruit and leaf extracts. The two organs were extracted by cold maceration with four solvents of increasing polarity: cyclohexane, dichloromethane, ethyl acetate and methanol. The extracts were screened for their chemical composition by HPLC-DAD to identify and quantify the phenolic compounds. Volatile compounds were detected by GC-MS. The antioxidant capacity through DPPH inhibition was tested. The anti-acetylcholinesterase, antibacterial and anti-proliferative activities were evaluated. Thirteen compounds, including 12 phenolics, were detected in the fruits, whereas 8 phenolic compounds were identified in the leaves. A total of 137 volatile compounds were identified in both organs. At 50 \u03bcg\/mL, the methanolic leaf extract presented the highest antioxidant capacity, with an inhibition percentage of 54.9%. The dichloromethane fruit extract reduced the acetylcholinesterase activity by 65.3%. The cyclohexane leaf extract reduced the proliferation of the HCT-116 cells by 54.8%, while the dichloromethane fruit extract exhibited the best inhibition against the Caco-2 cells (54%). Interestingly, the minimum inhibitory concentration (MIC) value of the cyclohexane fruit extract against Salmonella enterica serovar Kentucky was 2.4 \u03bcg\/mL, and the MIC value of the cyclohexane leaf extract against E. coli was 9.7 \u03bcg\/mL.<\/jats:p>","DOI":"10.3390\/plants12102001","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T01:52:41Z","timestamp":1684288361000},"page":"2001","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts"],"prefix":"10.3390","volume":"12","author":[{"given":"Michella","family":"Dawra","sequence":"first","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, 3136 Toulouse, France"},{"name":"Faculty of Agricultural Engineering and Veterinary Medicine, Lebanese University, Dekwaneh P.O. Box 6573, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8755-5436","authenticated-orcid":false,"given":"Nancy","family":"Nehme","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Engineering and Veterinary Medicine, Lebanese University, Dekwaneh P.O. Box 6573, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"El Beyrouthy","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Food Engineering, School of Engineering, Holy Spirit University of Kaslik, Jounieh BP 446, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Abi Rizk","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Food Engineering, School of Engineering, Holy Spirit University of Kaslik, Jounieh BP 446, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2106-6490","authenticated-orcid":false,"given":"Patricia","family":"Taillandier","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, 3136 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2439-5145","authenticated-orcid":false,"given":"Jalloul","family":"Bouajila","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, 3136 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssef","family":"El Rayess","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Food Engineering, School of Engineering, Holy Spirit University of Kaslik, Jounieh BP 446, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Salehi, B., Selamoglu, Z., Sener, B., Kilic, M., Jugran, A.K., De Tommasi, N., Sinisgalli, C., Milella, L., Rajkovic, J., and Morais-Braga, M.B.F. 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