{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:02:28Z","timestamp":1787029348472,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Science and Technological Development of the Republic of Serbia","award":["451-03-47\/2023-01\/200134"],"award-info":[{"award-number":["451-03-47\/2023-01\/200134"]}]},{"name":"Ministry of Education, Science and Technological Development of the Republic of Serbia","award":["142-451-3150\/2022-01\/01"],"award-info":[{"award-number":["142-451-3150\/2022-01\/01"]}]},{"name":"Provincial Secretariat for Higher Education and Scientific Research of APV Vojvodina","award":["451-03-47\/2023-01\/200134"],"award-info":[{"award-number":["451-03-47\/2023-01\/200134"]}]},{"name":"Provincial Secretariat for Higher Education and Scientific Research of APV Vojvodina","award":["142-451-3150\/2022-01\/01"],"award-info":[{"award-number":["142-451-3150\/2022-01\/01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Oilseed crops are widely cultivated and are related to nutrition and human health as valuable nutraceutical sources with valuable biological properties. The growing demand for oil plants used in human and animal nutrition or for the processing industry has contributed to the diversification and development of a new variety of oil crops. Increased oil crop diversity, besides ensuring reduced sensitivity to pests and climate conditions, has also led to improved nutritional values. In order to enable oil crop cultivation to become commercially sustainable, a comprehensive characterization of newly created varieties of oilseeds, including their nutritional and chemical composition, is required. In this study, two varieties of safflower and white and black mustard were investigated as alternative oil species for nutritional parameters, mainly protein, fat, carbohydrate, moisture, ash, polyphenols, flavonoids, chlorophylls contents, acids and mineral composition, and compared with those of two different genotypes of rapeseeds as a traditional oil crop plant. The proximate analysis found that the highest oil content was found in the oil rape NS Svetlana genotype (33.23%), while the lowest was in black mustard (25.37%). The protein content varies from around 26% in safflower samples to 34.63%, determined in white mustard. High content of unsaturated fatty acids and low content of saturated fatty acid was observed in the analyzed samples. In mineral analysis, the dominant elements were phosphorus, potassium, calcium and magnesium, in descending order. The observed oil crops are also good sources of microelements, including iron, copper, manganese and zinc, accompanied by high antioxidant activity due to the presence of significant amounts of polyphenolic and flavonoid compounds.<\/jats:p>","DOI":"10.3390\/plants12112160","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T03:02:23Z","timestamp":1685415743000},"page":"2160","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Comparative Study of the Nutritional and Chemical Composition of New Oil Rape, Safflower and Mustard Seed Varieties Developed and Grown in Serbia"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8892-3157","authenticated-orcid":false,"given":"Zorica S.","family":"Stojanovi\u0107","sequence":"first","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dajana D.","family":"Uletilovi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4056-2000","authenticated-orcid":false,"given":"Sne\u017eana \u017d.","family":"Kravi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2441-2045","authenticated-orcid":false,"given":"\u017darko S.","family":"Kevre\u0161an","sequence":"additional","affiliation":[{"name":"Institute of Food Technology in Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6635-3568","authenticated-orcid":false,"given":"Nada L.","family":"Grahovac","sequence":"additional","affiliation":[{"name":"Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana S.","family":"Lon\u010darevi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana D.","family":"\u0110urovi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Marjanovi\u0107 Jeromela","sequence":"additional","affiliation":[{"name":"Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1007\/s00253-012-4250-6","article-title":"Rapeseed and Sunflower Meal: A Review on Biotechnology Status and Challenges","volume":"95","author":"Lomascolo","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Raboanatahiry, N., Li, H., Yu, L., and Li, M. (2021). Rapeseed (Brassica napus): Processing, Utilization, and Genetic Improvement. Agronomy, 11.","DOI":"10.3390\/agronomy11091776"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1007\/s13197-015-2082-y","article-title":"Nutritional Value of Cold-Pressed Rapeseed Oil during Long Term Storage as Influenced by the Type of Packaging Material, Exposure to Light & Oxygen and Storage Temperature","volume":"53","author":"Wroniak","year":"2016","journal-title":"J. Food Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.clnu.2012.11.005","article-title":"Nutritional Evaluation of Rapeseed Protein Compared to Soy Protein for Quality, Plasma Amino Acids, and Nitrogen Balance-A Randomized Cross-over Intervention Study in Humans","volume":"32","author":"Fleddermann","year":"2013","journal-title":"Clin. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3731","DOI":"10.1002\/fsn3.1232","article-title":"Quality Evaluation of Rapeseed Oil in Chinese Traditional Stir-Frying","volume":"7","author":"Zhou","year":"2019","journal-title":"Food Sci. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/S0308-8146(97)00198-2","article-title":"Current Research Developments on Polyphenolics of Rapeseed\/Canola: A Review","volume":"62","author":"Naczk","year":"1998","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mayerhofer, M., Mayerhofer, R., Topinka, D., Christianson, J., and Good, A.G. (2011). Introgression Potential between Safflower (Carthamus tinctorius) and Wild Relatives of the Genus Carthamus. BMC Plant Biol., 11.","DOI":"10.1186\/1471-2229-11-47"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"46","DOI":"10.5937\/ratpov58-3150","article-title":"Seed Nutrients and Bioactive Compounds of Underuttilised Oil Crop Carthamus tinctorius L.","volume":"58","author":"Kiprovski","year":"2021","journal-title":"Ratar. I Povrt."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.fuel.2013.04.012","article-title":"Perspectives of Safflower Oil as Biodiesel Source for South Eastern Europe (Comparative Study: Safflower, Soybean and Rapeseed)","volume":"111","author":"Patrascoiu","year":"2013","journal-title":"Fuel"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/14786419.2014.971316","article-title":"Fatty Acid Composition and Tocopherol Profiles of Safflower (Carthamus tinctorius L.) Seed Oils","volume":"29","author":"Matthaus","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lietzow, J. (2021). Biologically Active Compounds in Mustard Seeds: A Toxicological Perspective. Foods, 10.","DOI":"10.3390\/foods10092089"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Grygier, A. (2022). Mustard Seeds as a Bioactive Component of Food. Food Rev. Int., 1\u201314.","DOI":"10.1080\/87559129.2021.2015774"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"112851","DOI":"10.1016\/j.indcrop.2020.112851","article-title":"Mustard Seed: Phenolic Composition and Effects on Lipid Oxidation in Oil, Oil-in-Water Emulsion and Oleogel","volume":"156","author":"Polak","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3505943","DOI":"10.1155\/2022\/3505943","article-title":"Proximate Composition, Physicochemical, and Lipids Profiling and Elemental Profiling of Rapeseed (Brassica napus L.) and Sunflower (Helianthus annuus L.) Grown in Morocco","volume":"2022","author":"Gagour","year":"2022","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1046\/j.1439-037X.2003.00053.x","article-title":"Effect of Planting Date on Seed Yield, Oil Content and Fatty Acid Composition of Safflower (Carthamus tinctorius) Cultivars Grown in the Mediterranean Region of Turkey","volume":"189","author":"Samanci","year":"2003","journal-title":"J. Agron. Crop Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/ijfs.12714","article-title":"Biochemical Characterisation of the Seed Oils of Four Safflower (Carthamus tinctorius) Varieties Grown in North-Eastern of Morocco","volume":"50","author":"Mansouri","year":"2015","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Sagan, A., Blicharz-Kania, A., Szmigielski, M., Andrejko, D., Sobczak, P., Zawi\u015blak, K., and Starek, A. (2019). Assessment of the Properties of Rapeseed Oil Enriched with Oils Characterized by High Content of \u03b1-Linolenic Acid. Sustainability, 11.","DOI":"10.3390\/su11205638"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e06294","DOI":"10.1016\/j.heliyon.2021.e06294","article-title":"Oxidative Stability of High Oleic Sunflower Oil during Deep-Frying Process of Purple Potato Purple Majesty","volume":"7","author":"Romano","year":"2021","journal-title":"Heliyon"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1080\/10942912.2014.968284","article-title":"Oil Content and Fatty Acids Composition in Brassica Species","volume":"18","author":"Sharafi","year":"2015","journal-title":"Int. J. Food Prop."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1007\/s11694-018-9848-7","article-title":"Identification of Bioactive Compounds and Total Phenol Contents of Cold Pressed Oils from Safflower and Camelina Seeds","volume":"12","year":"2018","journal-title":"J. Food Meas. Charact."},{"key":"ref_21","first-page":"59","article-title":"Evaluation of Oil Content and Fatty Acid Composition in Seeds of Different Genotypes of Safflower","volume":"2","author":"Vosoughkia","year":"2012","journal-title":"Int. J. Agric. Sci. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.foodchem.2016.06.102","article-title":"Fatty Acids and Sterols Composition, and Antioxidant Activity of Oils Extracted from Plant Seeds","volume":"213","year":"2016","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1700230","DOI":"10.1002\/ejlt.201700230","article-title":"Content of Erucic Acid in Edible Oils and Mustard by Quantitative 13 C NMR","volume":"120","author":"Kok","year":"2018","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"899076","DOI":"10.3389\/fpls.2022.899076","article-title":"A Review of Erucic Acid Production in Brassicaceae Oilseeds: Progress and Prospects for the Genetic Engineering of High and Low-Erucic Acid Rapeseeds (Brassica napus)","volume":"13","author":"Wang","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_25","unstructured":"Zealand, F.S.A.N. (2003). Erucic Acid in Food: A Toxicological Review and Risk Assessment. Canberra Food Stand. Aust. Newzeal., 17\u201323."},{"key":"ref_26","unstructured":"(2023, April 12). Euroepan Food Safety Authority EFSA\/Erucic Acid a Possible Health Risk for Highly Exposed Children. Available online: https:\/\/www.efsa.europa.eu\/en\/press\/news\/161109."},{"key":"ref_27","first-page":"4593","article-title":"Erucic Acid in Feed and Food","volume":"14","author":"Knutsen","year":"2016","journal-title":"EFSA J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1093\/ajcn\/79.4.582","article-title":"Diet and Risk of Ischemic Heart Disease in India","volume":"79","author":"Rastogi","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1007\/s13197-011-0540-8","article-title":"Storage Studies on Mustard Oil Blends","volume":"51","author":"Chugh","year":"2014","journal-title":"J. Food Sci. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"299","DOI":"10.3389\/fpls.2020.00299","article-title":"White Mustard (Sinapis Alba L.) Oil in Biodiesel Production: A Review","volume":"11","author":"Farooq","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Paciorek-Sadowska, J., Borowicz, M., Isbrandt, M., Czupry\u0144ski, B., and Apiecionek, \u0141. (2019). The Use of Waste from the Production of Rapeseed Oil for Obtaining of New Polyurethane Composites. Polymers, 11.","DOI":"10.3390\/polym11091431"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.foodhyd.2011.04.013","article-title":"Defatted Mustard Seed Meal-Based Biopolymer Film Development","volume":"26","author":"Hendrix","year":"2012","journal-title":"Food Hydrocoll."},{"key":"ref_33","first-page":"19","article-title":"Straw of White Mustard (Sinapis Alba) as an Alternative Raw Material in the Production of Particle Boards Resinated with UF Resin","volume":"10","author":"Dukarska","year":"2011","journal-title":"Acta Sci. Pol.-Silvarum Colendarum Ratio Ind. Lignaria"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"196","DOI":"10.5513\/JCEA01\/18.1.1879","article-title":"Mineral Composition of Winter Oilseed Rape (Brassica napus L.) Seeds as a Tool for Oil Yield Prognosis","volume":"18","author":"Szczepaniak","year":"2017","journal-title":"J. Cent. Eur. Agric."},{"key":"ref_35","first-page":"1","article-title":"Soil Minerals and Plant Nutrition","volume":"6","author":"Singh","year":"2015","journal-title":"Nat. Educ. Knowl."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/j.indcrop.2015.08.022","article-title":"Influence of Rapeseed Meal Treatments on Its Total Phenolic Content and Composition in Sinapine, Sinapic Acid and Canolol","volume":"76","author":"Zago","year":"2015","journal-title":"Ind. Crops Prod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"585","DOI":"10.4172\/2157-7110.1000585","article-title":"Chemical and Nutritional Aspects of Some Safflower Seed Varieties","volume":"7","author":"Ny","year":"2016","journal-title":"J. Food Process. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wang, Y., Meng, G., Chen, S., Chen, Y., Jiang, J., and Wang, Y.P. (2018). Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus. Molecules, 23.","DOI":"10.3390\/molecules23071815"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ahmad, P. (2017). Oilseed Crops: Yield and Adaptations under Environmental Stress, John Wiley & Sons Ltd.","DOI":"10.1002\/9781119048800"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1104\/pp.106.2.601","article-title":"Ethylene Production during Development of Mustard (Brassica iuncea) and Canola (Brassica napus) Seed","volume":"106","author":"Spence","year":"1994","journal-title":"Plant Physiol."},{"key":"ref_41","first-page":"S2379","article-title":"Possibility Use of Digital Image Analysis for the Estimation of the Rapeseed Maturity Stage","volume":"20","author":"Jankowski","year":"2018","journal-title":"Int. J. Food Prop."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF02670060","article-title":"The Relationship between Rapeseed Chlorophyll, Rapeseed Oil Chlorophyll and Percentage Green Seeds","volume":"59","author":"Daun","year":"1982","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_43","unstructured":"AOAC (2000). Official Methods of Analysis of the Association of Official Analytical Chemists, AOAC. [17th ed.]."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3945\/ajcn.112.035782","article-title":"Discrepancy between the Atwater Factor Predicted and Empirically Measured Energy Values of Almonds in Human Diets","volume":"96","author":"Novotny","year":"2012","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"139","DOI":"10.2298\/HEMIND101001078K","article-title":"Fatty Acid Composition Including Trans Isomers of Serbian Biscuits","volume":"65","author":"Kravic","year":"2011","journal-title":"Hem. Ind."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"803","DOI":"10.2298\/JSC090717051K","article-title":"Determination of Trans Fatty Acids in Foodstuffs by Gas Chromatography-Mass Spectrometry after Simultaneous Microwave-Assisted Extraction-Esterification","volume":"75","author":"Kravic","year":"2010","journal-title":"J. Serb. Chem. Soc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s00003-021-01329-x","article-title":"Fast and Sensitive Method for Phosphorus Determination in Dairy Products","volume":"16","author":"Rybicka","year":"2021","journal-title":"J. Consum. Prot. Food Saf."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.foodchem.2017.07.098","article-title":"Phenolic Profiles and Antioxidant Activity of Defatted Camelina and Sophia Seeds","volume":"240","author":"Rahman","year":"2018","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1042\/bst0110591","article-title":"Determinations of Total Carotenoids and Chlorophylls a and b of Leaf Extracts in Different Solvents","volume":"11","author":"Lichtenthaler","year":"1983","journal-title":"Biochem. Soc. Trans."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0176-1617(11)81192-2","article-title":"The Spectral Determination of Chlorophylls a and b, as Well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution","volume":"144","author":"Wellburn","year":"1994","journal-title":"J. Plant Physiol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/11\/2160\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:44:54Z","timestamp":1760125494000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/11\/2160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,30]]},"references-count":50,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["plants12112160"],"URL":"https:\/\/doi.org\/10.3390\/plants12112160","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,30]]}}}