{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T12:27:19Z","timestamp":1774873639335,"version":"3.50.1"},"reference-count":74,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System","award":["CARS\u201328\u201314"],"award-info":[{"award-number":["CARS\u201328\u201314"]}]},{"name":"China Agriculture Research System","award":["YJS20210207"],"award-info":[{"award-number":["YJS20210207"]}]},{"name":"Scientific Research Projects for Postgraduates of Anhui Universities","award":["CARS\u201328\u201314"],"award-info":[{"award-number":["CARS\u201328\u201314"]}]},{"name":"Scientific Research Projects for Postgraduates of Anhui Universities","award":["YJS20210207"],"award-info":[{"award-number":["YJS20210207"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Iron is an essential trace element for plants; however, low bioactive Fe in soil continuously places plants in an Fe-deficient environment, triggering oxidative damage. To cope with this, plants make a series of alterations to increase Fe acquisition; however, this regulatory network needs further investigation. In this study, we found notably decreased indoleacetic acid (IAA) content in chlorotic pear (Pyrus bretschneideri Rehd.) leaves caused by Fe deficiency. Furthermore, IAA treatment slightly induced regreening by increasing chlorophyll synthesis and Fe2+ accumulation. At that point, we identified PbrSAUR72 as a key negative effector output of auxin signaling and established its close relationship to Fe deficiency. Furthermore, the transient PbrSAUR72 overexpression could form regreening spots with increased IAA and Fe2+ content in chlorotic pear leaves, whereas its transient silencing does the opposite in normal pear leaves. In addition, cytoplasm-localized PbrSAUR72 exhibits root expression preferences and displays high homology to AtSAUR40\/72. This promotes salt tolerance in plants, indicating a putative role for PbrSAUR72 in abiotic stress responses. Indeed, transgenic plants of Solanum lycopersicum and Arabidopsis thaliana overexpressing PbrSAUR72 displayed less sensitivity to Fe deficiency, accompanied by substantially elevated expression of Fe-induced genes, such as FER\/FIT, HA, and bHLH39\/100. These result in higher ferric chelate reductase and root pH acidification activities, thereby hastening Fe absorption in transgenic plants under an Fe-deficient condition. Moreover, the ectopic overexpression of PbrSAUR72 inhibited reactive oxygen species production in response to Fe deficiency. These findings contribute to a new understanding of PbrSAURs and its involvement in Fe deficiency, providing new insights for the further study of the regulatory mechanisms underlying the Fe deficiency response.<\/jats:p>","DOI":"10.3390\/plants12112173","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T02:27:30Z","timestamp":1685500050000},"page":"2173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evidence That PbrSAUR72 Contributes to Iron Deficiency Tolerance in Pears by Facilitating Iron Absorption"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9768-5533","authenticated-orcid":false,"given":"Guoling","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"},{"name":"Agricultural Experimental Center of Guiyang, Guiyang Agriculture and Rural Bureau, Guiyang 550018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"},{"name":"Singleron Biotechnology Co., Ltd., Nanjing 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiyu","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuqin","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomei","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lun","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8363-4495","authenticated-orcid":false,"given":"Wei","family":"Heng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7735-8527","authenticated-orcid":false,"given":"Liwu","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2681-0426","authenticated-orcid":false,"given":"Bing","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fruit Biology, School of Horticulture, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1104\/pp.104.3.815","article-title":"Iron: Nutritious, noxious, and not readily available","volume":"104","author":"Guerinot","year":"1994","journal-title":"Plant Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.tplants.2014.07.005","article-title":"Iron nutrition, biomass production, and plant product quality","volume":"20","author":"Briat","year":"2015","journal-title":"Trends Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.ecoenv.2015.06.019","article-title":"Assessment of heavy metal pollution and human health risk in urban soils of steel industrial city (Anshan), Liaoning, Northeast China","volume":"120","author":"Xiao","year":"2015","journal-title":"Ecotox. Environ. Safe"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/nph.14364","article-title":"New routes for plant iron mining","volume":"214","author":"Curie","year":"2017","journal-title":"New Phytol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.envexpbot.2010.12.012","article-title":"Effects of moderate and severe iron deficiency chlorosis on fruit yield, appearance and composition in pear (Pyrus communis L.) and peach (Prunus persica (L.) Batsch)","volume":"71","author":"Melgar","year":"2011","journal-title":"Environ. Exp. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.tplants.2014.11.004","article-title":"Molecular mechanisms governing Arabidopsis iron uptake","volume":"20","author":"Brumbarova","year":"2015","journal-title":"Trends Plant Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1002\/csc2.20661","article-title":"Iron deficiency in soybean","volume":"62","author":"Merry","year":"2022","journal-title":"Crop Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Li, Q., Chen, L., and Yang, A. (2020). The molecular mechanisms underlying iron deficiency responses in rice. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010043"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s11103-019-00896-w","article-title":"Common and specific responses to iron and phosphorus deficiencies in roots of apple tree (Malus \u00d7 domestica)","volume":"101","author":"Valentinuzzi","year":"2019","journal-title":"Plant Mol. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zamboni, A., Zanin, L., Tomasi, N., Pezzotti, M., Pinton, R., Varanini, Z., and Cesco, S. (2012). Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-101"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0248-4900(96)81320-7","article-title":"Cellular and molecular aspects of iron metabolism in plants","volume":"84","author":"Briat","year":"1995","journal-title":"Biol. Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1039\/C7MT00136C","article-title":"Iron homeostasis in plants\u2014A brief overview","volume":"9","author":"James","year":"2017","journal-title":"Metallomics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/mp\/ssr065","article-title":"Fitting into the harsh reality: Regulation of iron-deficiency responses in dicotyledonous plants","volume":"5","author":"Ivanov","year":"2012","journal-title":"Mol. Plant"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/17800","article-title":"A ferric-chelate reductase for iron uptake from soils","volume":"397","author":"Robinson","year":"1999","journal-title":"Nature"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1105\/tpc.001388","article-title":"IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth","volume":"14","author":"Vert","year":"2002","journal-title":"Plant Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.pbi.2020.05.006","article-title":"Computational solutions for modeling and controlling plant response to abiotic stresses: A review with focus on iron deficiency","volume":"57","author":"Tong","year":"2020","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1111\/nph.15753","article-title":"Transcriptional integration of the responses to iron availability in Arabidopsis by the bHLH factor ILR3","volume":"223","author":"Tissot","year":"2019","journal-title":"New Phytol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3389\/fpls.2019.00006","article-title":"The transcriptional control of iron homeostasis in plants: A tale of bHLH transcription factors","volume":"10","author":"Gao","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1093\/jxb\/erab003","article-title":"All together now: Regulation of the iron deficiency","volume":"72","author":"Riaz","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"677611","DOI":"10.3389\/fpls.2021.677611","article-title":"Regulatory mechanisms of bHLH transcription factors in plant adaptive responses to various abiotic stresses","volume":"12","author":"Qian","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1093\/jxb\/err314","article-title":"Induction of root Fe(III) reductase activity and proton extrusion by iron deficiency is mediated by auxin-based systemic signaling in Malus xiaojinensis","volume":"63","author":"Wu","year":"2012","journal-title":"J. Exp. Bot."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.plaphy.2011.02.018","article-title":"Auxin: A major player in the shoot-to-root regulation of root Fe-stress physiological responses to Fe deficiency in cucumber plants","volume":"49","author":"Bacaicoa","year":"2011","journal-title":"Plant Physiol. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"110285","DOI":"10.1016\/j.ecoenv.2020.110285","article-title":"Gamma-aminobutyric acid enhances tolerance to iron deficiency by stimulating auxin signaling in cucumber (Cucumis sativus L.)","volume":"192","author":"Guo","year":"2020","journal-title":"Ecotox. Environ. Safe"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.3389\/fpls.2017.02169","article-title":"The interaction between auxin and nitric oxide regulates root growth in response to iron deficiency in rice","volume":"8","author":"Sun","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1104\/pp.112.212787","article-title":"SAUR36, a small auxin up gene, is involved in the promotion of leaf senescence in Arabidopsis","volume":"161","author":"Hou","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1104\/pp.109.143875","article-title":"SAUR39, a small auxin up RNA gene, acts as a negative regulator of auxin synthesis and transport in rice","volume":"151","author":"Kant","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3391","DOI":"10.1093\/jxb\/erx184","article-title":"FRUITFULL controls SAUR10 expression and regulates Arabidopsis growth and architecture","volume":"68","author":"Bemer","year":"2017","journal-title":"J. Exp. Bot."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1093\/pcp\/pcz231","article-title":"SAUR49 can positively regulate leaf senescence by suppressing SSPP in Arabidopsis","volume":"61","author":"Wen","year":"2020","journal-title":"Plant Cell Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/nph.15956","article-title":"Natural variations of growth thermo-responsiveness determined by SAUR26\/27\/28 proteins in Arabidopsis thaliana","volume":"224","author":"Wang","year":"2019","journal-title":"New Phytol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1093\/aob\/mcz160","article-title":"The SAUR41 subfamily of small auxin up RNA genes is abscisic acid-inducible to modulate cell expansion and salt tolerance in Arabidopsis thaliana seedlings","volume":"125","author":"Qiu","year":"2020","journal-title":"Ann. Bot."},{"key":"ref_31","first-page":"595","article-title":"Analysis of endogenous IAA content and signaling genes expression in retrieved leaves of \u2018Dangshansuli\u2019 Pear (Pyrus bretschneideri Rehd.)","volume":"41","author":"Jia","year":"2021","journal-title":"Acta Bot. Boreali-Occident. Sin."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.tplants.2011.03.007","article-title":"ROS signaling: The new wave","volume":"16","author":"Mittler","year":"2011","journal-title":"Trends Plant Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.tplants.2004.08.009","article-title":"Reactive oxygen gene network of plants","volume":"9","author":"Mittler","year":"2004","journal-title":"Trends Plant Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1146\/annurev-phyto-080516-035537","article-title":"Iron and immunity","volume":"55","author":"Verbon","year":"2017","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1104\/pp.110.161109","article-title":"Nitric oxide acts downstream of auxin to trigger root ferric-chelate reductase activity in response to iron deficiency in Arabidopsis","volume":"154","author":"Chen","year":"2010","journal-title":"Plant Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"167","DOI":"10.4161\/psb.6.1.14111","article-title":"Latest findings about the interplay of auxin, ethylene and nitric oxide in the regulation of Fe deficiency responses by strategy I plants","volume":"6","author":"Romera","year":"2011","journal-title":"Plant Signal. Behav."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/jxb\/ery332","article-title":"The SAUR gene family: The plant\u2019s toolbox for adaptation of growth and development","volume":"70","author":"Stortenbeker","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1016\/S2095-3119(19)62651-1","article-title":"TaSAUR78 enhances multiple abiotic stress tolerance by regulating the interacting gene TaVDAC1","volume":"18","author":"Guo","year":"2019","journal-title":"J. Integr. Agric."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"625493","DOI":"10.3389\/fpls.2021.625493","article-title":"The Arabidopsis small auxin up RNA32 protein regulates ABA-mediated responses to drought stress","volume":"12","author":"He","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1111\/jipb.12984","article-title":"AtHAP5A regulates iron translocation in iron-deficient Arabidopsis thaliana","volume":"62","author":"Zhu","year":"2020","journal-title":"J. Integr. Plant Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1093\/jxb\/eraa547","article-title":"MxMPK6-2-bHLH104 interaction is involved in reactive oxygen species signaling in response to iron deficiency in apple rootstock","volume":"72","author":"Li","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1111\/jipb.13251","article-title":"The miR157-SPL-CNR module acts upstream of bHLH101 to negatively regulate iron deficiency responses in tomato","volume":"64","author":"Zhu","year":"2022","journal-title":"J. Integr. Plant Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.scienta.2018.09.077","article-title":"Expression of two indole-3-acetic acid (IAA)-amido synthetase (GH3) genes during fruit development of raspberry (Rubus idaeus Heritage)","volume":"246","author":"Bernale","year":"2019","journal-title":"Sci. Hortic."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"110955","DOI":"10.1016\/j.postharvbio.2019.110955","article-title":"Ethylene promotes IAA reduction through PuERFs-activated PuGH3.1 during fruit ripening in pear (Pyrus ussuriensis)","volume":"157","author":"Yue","year":"2019","journal-title":"Postharvest Biol. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"13938","DOI":"10.1073\/pnas.212448699","article-title":"The tomato fer gene encoding a bHLH protein controls iron-uptake responses in roots","volume":"99","author":"Ling","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3400","DOI":"10.1105\/tpc.104.024315","article-title":"The essential basic helix-loop-helix protein FIT1 is required for the iron deficiency response","volume":"16","author":"Colangelo","year":"2004","journal-title":"Plant Cell"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1038\/sj.cr.7290331","article-title":"AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants","volume":"15","author":"Yuan","year":"2005","journal-title":"Cell Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"844","DOI":"10.3389\/fpls.2019.00844","article-title":"FIT-binding proteins and their functions in the regulation of Fe homeostasis","volume":"10","author":"Wu","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_49","first-page":"480172","article-title":"Why FIT and bHLH Ib interdependently regulate Fe-uptake","volume":"2","author":"Cai","year":"2022","journal-title":"Mol. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/cr.2008.26","article-title":"FIT interacts with AtbHLH38 and AtbHLH39 in regulating iron uptake gene expression for iron homeostasis in Arabidopsis","volume":"18","author":"Yuan","year":"2008","journal-title":"Cell Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1093\/mp\/sss089","article-title":"Requirement and functional redundancy of Ib subgroup bHLH proteins for iron deficiency responses and uptake in Arabidopsis thaliana","volume":"6","author":"Wang","year":"2013","journal-title":"Mol. Plant"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1146\/annurev.arplant.52.1.817","article-title":"Plant plasma membrane H+-ATPases: Powerhouses for nutrient uptake","volume":"52","author":"Palmgren","year":"2001","journal-title":"Annu. Rev. Plant Physiol. Plant Mol. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1023\/A:1019942200164","article-title":"Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects","volume":"50","author":"Henriques","year":"2002","journal-title":"Plant Mol. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.plaphy.2007.03.006","article-title":"FIT, the FER-like iron deficiency induced transcription factor in Arabidopsis","volume":"45","author":"Bauer","year":"2007","journal-title":"Plant Physiol. Biochem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1016\/j.molp.2018.06.005","article-title":"Four IVa bHLH transcription factors are novel interactors of FIT and mediate JA inhibition of iron uptake in Arabidopsis","volume":"11","author":"Cui","year":"2018","journal-title":"Mol. Plant"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.tplants.2016.08.002","article-title":"ROS are good","volume":"22","author":"Mittler","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1111\/tpj.13299","article-title":"Reactive oxygen species, abiotic stress and stress combination","volume":"90","author":"Choudhury","year":"2017","journal-title":"Plant J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.tplants.2011.10.001","article-title":"A burst of plant NADPH oxidases","volume":"17","author":"Marino","year":"2012","journal-title":"Trends Plant Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1093\/jxb\/eraa522","article-title":"Reactive oxygen species coordinate the transcriptional responses to iron availability in Arabidopsis","volume":"72","author":"Mark","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Song, H., Chen, F., Wu, X., Hu, M., Geng, Q.-L., Ye, M., Zhang, C., Jiang, L., and Cao, S.-Q. (2022). MNB1 gene is involved in regulating the iron-deficiency stress response in Arabidopsis thaliana. BMC Plant Biol., 22.","DOI":"10.1186\/s12870-022-03553-5"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/S0031-9422(00)81791-4","article-title":"Elisa determination of IAA using antibodies against ring-linked IAA","volume":"26","author":"Pence","year":"1987","journal-title":"Phytochemistry"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1104\/pp.61.1.107","article-title":"Cucumber seedling indoleacetaldehyde oxidase","volume":"61","author":"Bower","year":"1978","journal-title":"Plant Physiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"15823","DOI":"10.1016\/S0021-9258(19)49609-9","article-title":"Purification and characterization of indolepyruvate decarboxylase. A novel enzyme for indole\u20133\u2013acetic acid biosynthesis in Enterobacter cloacae","volume":"267","author":"Koga","year":"1992","journal-title":"J. Biol. Chem."},{"key":"ref_64","first-page":"54","article-title":"Relativity of active iron contents and chlorosis in the Kiwis leaf","volume":"11","author":"Mao","year":"2002","journal-title":"Acta Agric. Boreali-Occident. Sin."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1007\/s00425-018-2949-z","article-title":"LeSPL\u2013CNR negatively regulates Cd acquisition through repressing nitrate reductase-mediated nitric oxide production in tomato","volume":"248","author":"Chen","year":"2018","journal-title":"Planta"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"15","DOI":"10.21769\/BioProtoc.843","article-title":"Determination of ferric chelate reductase activity in the Arabidopsis thaliana root","volume":"3","author":"Aksoy","year":"2013","journal-title":"Bio-Protocol"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S1360-1385(03)00048-7","article-title":"Iron solutions: Acquisition strategies and signaling pathways in plants","volume":"8","author":"Schmidt","year":"2003","journal-title":"Trends Plant Sci."},{"key":"ref_68","first-page":"129","article-title":"Enhanced tolerance to photooxidative stress of transgenic Nicotiana tabacum with high chloroplastic glutathione reductase activity","volume":"34","author":"Aono","year":"1993","journal-title":"Plant Cell Physiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"8","DOI":"10.21769\/BioProtoc.1108","article-title":"Histochemical detection of superoxide and H2O2 accumulation in Brassica juncea seedlings","volume":"4","author":"Kumar","year":"2014","journal-title":"Bio-Protocol"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1023\/A:1004876712476","article-title":"Cell wall peroxidase activity, hydrogen peroxidase level and NaCl-inhibited root growth of rice seedling","volume":"230","author":"Lin","year":"2001","journal-title":"Plant Soil"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1111\/pbi.13114","article-title":"BBX16, a B-box protein, positively regulates light-induced anthocyanin accumulation by activating MYB10 in red pear","volume":"17","author":"Bai","year":"2019","journal-title":"Plant Biotechol. J."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1038\/nbt0787-726","article-title":"Efficient transfer of a glyphosate tolerance gene into tomato using a binary agrobacterium tumefaciens vector","volume":"5","author":"Fillatti","year":"1987","journal-title":"Nat. Biotechol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1046\/j.1365-313x.1998.00343.x","article-title":"Floral dip: A simplified method for agrobacterium-mediated transformation of Arabidopsis thaliana","volume":"16","author":"Clough","year":"1998","journal-title":"Plant J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR","volume":"25","author":"Livak","year":"2002","journal-title":"Methods"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/11\/2173\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:45:34Z","timestamp":1760125534000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/11\/2173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,30]]},"references-count":74,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["plants12112173"],"URL":"https:\/\/doi.org\/10.3390\/plants12112173","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,30]]}}}