{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:31:38Z","timestamp":1790875898092,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,11]],"date-time":"2023-06-11T00:00:00Z","timestamp":1686441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>According to the WHO, the overall age-standardized cancer rate keeps declining, and the number of cases diagnosed each year increases, remaining among the leading causes of death in 91 out of 172 recorded countries. In this context, novel cancer prediction and therapeutic protocols are compulsory. The effect of a Stachys circinata L\u2019H\u00e9r dichloromethane extract (ScDME) on cell redox homeostasis and tumor proliferation was investigated. HepG2 cell feedback mechanisms to oxidative stress exposure were evaluated by determining catalase (CAT) and reduced glutathione (GSH), following the supply with ScDME (0.0\u20135.7 \u00b5g\/\u00b5L). Cytotoxicity of ScDME against the human umbilical vein endothelial cell (HUVEC) and two human cancer cell lines (breast: MCF7; liver: HepG2) was evaluated by the MTT assay. H2O2-stressed HepG2 cells supplied with the S. circinata extracts exhibited significantly increased CAT and GSH activity as compared to unsupplied ones. The anti-inflammatory activity of the extracts was evaluated by real time-qPCR on IL-1, IL-6 and TNF-\u03b1 expression. As a result, this research points out that S. circinata dichloromethane extract owns anti-inflammatory and anti-proliferative properties against MCF7 and HepG2 cells and activates CAT and GSH of the HepG2 cells\u2019 antioxidant enzyme system.<\/jats:p>","DOI":"10.3390\/plants12122272","type":"journal-article","created":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T01:59:07Z","timestamp":1686535147000},"page":"2272","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Antioxidant, Anti-Inflammatory and Anti-Proliferative Properties of Stachys circinata on HepG2 and MCF7 Cells"],"prefix":"10.3390","volume":"12","author":[{"given":"Wassila","family":"Slimani","sequence":"first","affiliation":[{"name":"Laboratoire d\u2019Obtention de Substances Th\u00e9rapeutiques (L.O.S.T), D\u00e9partement de Chimie, Universit\u00e9 des Fr\u00e8res Mentouri-Constantine, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0187-4968","authenticated-orcid":false,"given":"Margherita","family":"Maioli","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/B, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8632-2577","authenticated-orcid":false,"given":"Sara","family":"Cruciani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/B, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakina","family":"Zerizer","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019Obtention de Substances Th\u00e9rapeutiques (L.O.S.T), D\u00e9partement de Chimie, Universit\u00e9 des Fr\u00e8res Mentouri-Constantine, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Santaniello","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/B, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahia","family":"Kabouche","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019Obtention de Substances Th\u00e9rapeutiques (L.O.S.T), D\u00e9partement de Chimie, Universit\u00e9 des Fr\u00e8res Mentouri-Constantine, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Coradduzza","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/B, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9218-6863","authenticated-orcid":false,"given":"Mario","family":"Chessa","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Pharmacy, Sassari University, Viale San Pietro 43\/b, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Fancello","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Pharmacy, Sassari University, Viale San Pietro 43\/b, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana","family":"Migheli","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Pharmacy, Sassari University, Viale San Pietro 43\/b, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pier Andrea","family":"Serra","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Pharmacy, Sassari University, Viale San Pietro 43\/b, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"D\u2019hallewin","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, National Research Council, Traversa la Crucca, 3. 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