{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:49:17Z","timestamp":1782402557733,"version":"3.54.5"},"reference-count":65,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T00:00:00Z","timestamp":1687392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Scientific Research at Umm Al-Qura University","award":["23UQU4320529DSR001"],"award-info":[{"award-number":["23UQU4320529DSR001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Premna, a genus consisting of approximately 200 species, predominantly thrives in tropical and subtropical areas. Many of these species have been utilized in ethnopharmacology for diverse medicinal applications. In Saudi Arabia, Premna resinosa (Hochst.) Schauer (Lamiaceae) grows wildly, and its slightly viscid leaves are attributed to the production of leaf accession. In this study, we aimed to extract the surface accession from fresh leaves using dichloromethane to evaluate the anticancer potential. The plant exudate yielded two previously unknown labdane diterpenes, Premnaresone A and B, in addition to three already described congeners and four known flavonoids. The isolation process was accomplished using a combination of silica gel column chromatography and semi-preparative HPLC, the structures of which were identified by NMR and HRESIMS analyses and a comparison with the literature data of associated compounds. Furthermore, we employed a density functional theory (DFT)\/NMR approach to suggest the relative configuration of different compounds. Consequently, we investigated the possibility of developing new chaperone inhibitors by subjecting diterpenes 1\u20135 to a Surface Plasmon Resonance-screening, based on the knowledge that oridonin, a diterpene, interacts with Heat Shock Protein 70 (Hsp70) 1A in cancer cells. Additionally, we studied the anti-proliferative activity of compounds 1\u20135 on human Jurkat (human T-cell lymphoma) and HeLa (epithelial carcinoma) cell lines, where diterpene 3 exhibited activity in Jurkat cell lines after 48 h, with an IC50 of 15.21 \u00b1 1.0 \u00b5M. Molecular docking and dynamic simulations revealed a robust interaction between compound 3 and Hsp70 key residues.<\/jats:p>","DOI":"10.3390\/plants12132421","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T01:54:56Z","timestamp":1687485296000},"page":"2421","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring the Anticancer Potential of Premna resinosa (Hochst.) Leaf Surface Extract: Discovering New Diterpenes as Heat Shock Protein 70 (Hsp70) Binding Agents"],"prefix":"10.3390","volume":"12","author":[{"given":"Valentina","family":"Parisi","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Donadio","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Laura","family":"Bellone","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumia","family":"Belaabed","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Research Unit, Development of Natural Resources, Bioactive Molecules Physicochemical and Biological Analysis, University Brothers Mentouri, Route Ain ElBey, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2201-7201","authenticated-orcid":false,"given":"Ammar","family":"Bader","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-7732","authenticated-orcid":false,"given":"Angela","family":"Bisio","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4883-4840","authenticated-orcid":false,"given":"Valeria","family":"Iobbi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Gazzillo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Giovanna","family":"Chini","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Territory, University of Molise, C.da Fonte Lappone, 86090 Pesche, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Bifulco","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5274-8633","authenticated-orcid":false,"given":"Immacolata","family":"Faraone","sequence":"additional","affiliation":[{"name":"Department of Science, University of Basilicata, Viale dell\u2019Ateneo Lucano 10, 85100 Potenza, Italy"},{"name":"Innovative Startup Farmis s.r.l., Via Nicola Vaccaro 40, 85100 Potenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7937-3309","authenticated-orcid":false,"given":"Antonio","family":"Vassallo","sequence":"additional","affiliation":[{"name":"Department of Science, University of Basilicata, Viale dell\u2019Ateneo Lucano 10, 85100 Potenza, Italy"},{"name":"Spinoff TNcKILLERS s.r.l., Viale dell\u2019Ateneo Lucano 10, 85100 Potenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1080\/13880209.2017.1323225","article-title":"Ethnomedicinal uses, phytochemistry and pharmacological aspects of the genus Premna: A review","volume":"55","author":"Dianita","year":"2017","journal-title":"Pharm. 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