{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T03:10:57Z","timestamp":1775617857078,"version":"3.50.1"},"reference-count":113,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T00:00:00Z","timestamp":1689638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chief Scientist Fund of the Ministry of Agriculture in Israel","award":["20-03-0124"],"award-info":[{"award-number":["20-03-0124"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Recent studies demonstrated a significant impact of some major macronutrients on function and production of medical cannabis plants, yet information on the effect of most nutrients, including Mg, is scarce. Magnesium is required for major physiological functions and metabolic processes in plants, and in the present study we studied the effects of five Mg treatments (2, 20, 35, 70, and 140 mg L\u22121 Mg), on plant development and function, and distribution of minerals in drug-type (medical) cannabis plants, at the vegetative growth phase. The plants were cultivated in pots under controlled environment conditions. The results demonstrate that plant development is optimal under Mg supply of 35\u201370 mg L\u22121 (ppm), and impaired under lower Mg input of 2\u201320 mg L\u22121. Two mg L\u22121 Mg resulted in visual deficiency symptoms, shorter plants, reduced photosynthesis rate, transpiration rate, photosynthetic pigments and stomatal conduction in young-mature leaves, and a 28% reduction of total plant biomass compared to the optimal supply of 35 mg L\u22121 Mg. The highest supply level of 140 mg L\u22121 Mg induced a small decrease in physiological function, which did not affect morphological development and biomass accumulation. The low-deficient Mg supply of 2 mg L\u22121 Mg stimulated Mg uptake and accumulation of N, P, K, Ca, Mn, and Zn in the plant. Increased Mg supply impaired uptake of Ca and K and their root-to-shoot translocation, demonstrating competitive cation inhibition. Mg-deficiency symptoms developed first in old leaves (at 2 mg L\u22121 Mg) and progressed towards young-mature leaves, demonstrating ability for Mg in-planta storage and remobilization. Mg toxicity symptoms appeared in old leaves from the bottom of the plants, under 140 mg L\u22121 Mg. Taken together, the findings suggest 35\u201370 mg L\u22121 Mg as the optimal concentration range for cannabis plant development and function at the vegetative growth phase.<\/jats:p>","DOI":"10.3390\/plants12142676","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T01:35:16Z","timestamp":1689644116000},"page":"2676","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Response of Medical Cannabis to Magnesium (Mg) Supply at the Vegetative Growth Phase"],"prefix":"10.3390","volume":"12","author":[{"given":"Dalit","family":"Morad","sequence":"first","affiliation":[{"name":"Institute of Soil Water and Environmental Sciences, Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel"},{"name":"The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirit","family":"Bernstein","sequence":"additional","affiliation":[{"name":"Institute of Soil Water and Environmental Sciences, Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1002\/cbdv.200790144","article-title":"History of cannabis and its preparations in saga, science, and sobriquet","volume":"4","author":"Russo","year":"2007","journal-title":"Chem. Biodivers."},{"key":"ref_2","first-page":"31","article-title":"Phenological growth stages of hemp (Cannabis sativa L.): Codification and description according to the BBCH scale","volume":"24","author":"Mishchenko","year":"2017","journal-title":"\u017dem\u0117s \u016bkio Moksl."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1590\/S1516-44462006000200015","article-title":"History of cannabis as a medicine: A review","volume":"28","author":"Zuardi","year":"2006","journal-title":"Rev. Bras. Psiquiatr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.indcrop.2018.11.039","article-title":"Interplay between chemistry and morphology in medical cannabis (Cannabis sativa L.)","volume":"129","author":"Bernstein","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chandra, S., Lata, H., and ElSohly, M.A. (2017). Cannabis sativa L.\u2014Botany and Biotechnology, Springer International Publishing.","DOI":"10.1007\/978-3-319-54564-6"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1139\/cjps-2018-0038","article-title":"Vegetative propagation of cannabis by stem cuttings: Eeffects of leaf number, cutting position, rooting hormone, and leaf tip removal","volume":"98","author":"Caplan","year":"2018","journal-title":"Can. J. Plant Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"657323","DOI":"10.3389\/fpls.2021.657323","article-title":"The highs and lows of P supply in medical cannabis: Effects on cannabinoids, the ionome, and morpho-physiology","volume":"12","author":"Shiponi","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"113154","DOI":"10.1016\/j.indcrop.2020.113154","article-title":"Response of medical cannabis (Cannabis sativa L.) genotypes to P supply under long photoperiod: Functional phenotyping and the ionome","volume":"161","author":"Shiponi","year":"2021","journal-title":"Ind. Crops Prod."},{"key":"ref_9","unstructured":"Kirkby, E. (2012). Marschner\u2019s Mineral Nutrition of Higher Plants, Elsevier."},{"key":"ref_10","unstructured":"Hawkesford, M., Horst, W., Kichey, T., Lambers, H., Schjoerring, J., M\u00f8ller, I.S., and White, P. (2012). Marschner\u2019s Mineral Nutrition of Higher Plants, Elsevier."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1093\/pcp\/pcu156","article-title":"Strategies for optimization of mineral nutrient transport in plants: Multilevel regulation of nutrient-dependent dynamics of root architecture and transporter activity","volume":"55","author":"Aibara","year":"2014","journal-title":"Plant Cell Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1080\/01904160802116068","article-title":"The role of nutrient efficient plants in improving crop yields in the twenty first century","volume":"31","author":"Fageria","year":"2008","journal-title":"J. Plant Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.3389\/fpls.2019.01369","article-title":"Response of medical cannabis (Cannabis sativa L.) genotypes to K supply under long photoperiod","volume":"10","author":"Saloner","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"572293","DOI":"10.3389\/fpls.2020.572293","article-title":"Response of medical cannabis (Cannabis sativa L.) to nitrogen supply under long photoperiod","volume":"11","author":"Saloner","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Saloner, A., and Bernstein, N. (2022). Effect of potassium (K) supply on cannabinoids, terpenoids and plant function in medical cannabis. Agronomy, 12.","DOI":"10.3390\/agronomy12051242"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"113516","DOI":"10.1016\/j.indcrop.2021.113516","article-title":"Nitrogen supply affects cannabinoid and terpenoid profile in medical cannabis (Cannabis sativa L.)","volume":"167","author":"Saloner","year":"2021","journal-title":"Ind. Crops Prod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"736","DOI":"10.3389\/fpls.2019.00736","article-title":"Impact of N, P, K, and humic acid supplementation on the chemical profile of medical cannabis (Cannabis sativa L.)","volume":"10","author":"Bernstein","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.3389\/fpls.2021.764103","article-title":"Optimisation of nitrogen, phosphorus, and potassium for soilless production of Cannabis sativa in the flowering stage using response surface analysis","volume":"12","author":"Bevan","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.semcdb.2017.08.005","article-title":"Functional dissection and transport mechanism of magnesium in plants","volume":"74","author":"Chen","year":"2018","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.1105\/tpc.010352","article-title":"A Novel family of magnesium transport genes in arabidopsis","volume":"13","author":"Li","year":"2001","journal-title":"Plant Cell Online"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1021\/bi00648a012","article-title":"The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications","volume":"15","author":"Lorimer","year":"1976","journal-title":"Biochemistry"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s11104-013-1589-0","article-title":"Physiological and molecular responses to magnesium nutritional imbalance in plants","volume":"368","author":"Verbruggen","year":"2013","journal-title":"Plant Soil"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"795","DOI":"10.21273\/HORTSCI12947-18","article-title":"Effects of nitrogen, phosphorus, potassium, calcium, or magnesium deficiency on growth and photosynthesis of eustoma","volume":"53","author":"Chen","year":"2018","journal-title":"HortScience"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1023\/A:1016091118585","article-title":"Magnesium transport and function in plants: The tip of the iceberg","volume":"15","author":"Shaul","year":"2002","journal-title":"BioMetals"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1023\/A:1016022730880","article-title":"Structural and catalytic chemistry of magnesium-dependent enzymes","volume":"15","author":"Cowan","year":"2002","journal-title":"BioMetals"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.cj.2015.11.003","article-title":"Magnesium deficiency in plants: An urgent problem","volume":"4","author":"Guo","year":"2016","journal-title":"Crop J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s11104-016-2886-1","article-title":"Magnesium deficiency decreases biomass water-use efficiency and increases leaf water-use efficiency and oxidative stress in barley plants","volume":"406","author":"Tavakol","year":"2016","journal-title":"Plant Soil"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/j.1469-8137.2010.03257.x","article-title":"Systems analysis of the responses to long-term magnesium deficiency and restoration in Arabidopsis thaliana","volume":"187","author":"Hermans","year":"2010","journal-title":"New Phytol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1093\/jxb\/eri215","article-title":"Physiological characterization of Mg deficiency in Arabidopsis thaliana","volume":"56","author":"Hermans","year":"2005","journal-title":"J. Exp. Bot."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/BF02374887","article-title":"Ionic interactions in rice plants from dilute solutions","volume":"70","author":"Fageria","year":"1983","journal-title":"Plant Soil"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1097\/00010694-196606000-00003","article-title":"EL Some observations on the interrelationships of potassium and magnesium","volume":"101","author":"Omar","year":"1966","journal-title":"Soil Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1081\/PLN-100106981","article-title":"Nutrient interactions in crop plants","volume":"24","author":"Fageria","year":"2001","journal-title":"J. Plant Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.21273\/HORTSCI.40.6.1879","article-title":"Effects of Supplied Nitrogen Form and Quantity on Growth and Postharvest Quality of Ranunculus asiaticus Flowers","volume":"40","author":"Bernstein","year":"2005","journal-title":"Hortscience"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.freeradbiomed.2010.06.032","article-title":"Free radical biology & medicine involvement of the plant antioxidative response in the differential growth sensitivity to salinity of leaves vs roots during cell development","volume":"49","author":"Bernstein","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1042\/bst0110591","article-title":"Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents","volume":"11","author":"Lichtenthaler","year":"1983","journal-title":"Biochem. Soc. Trans."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Bhatla, S.C., and Lal, M.A. (2018). Plant Physiology, Development and Metabhatla, Springer.","DOI":"10.1007\/978-981-13-2023-1"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1071\/CP15104","article-title":"Role of magnesium rertilisers in agriculture: Plant-soil continuum","volume":"66","author":"Senbayram","year":"2015","journal-title":"Crop Pasture Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.cj.2020.10.005","article-title":"Synergistic and antagonistic interactions between potassium and magnesium in higher plants","volume":"9","author":"Xie","year":"2021","journal-title":"Crop J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e992287","DOI":"10.4161\/15592324.2014.992287","article-title":"Magnesium stress signaling in plant: Just a beginning","volume":"10","author":"Guo","year":"2015","journal-title":"Plant Signal. Behav."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"830224","DOI":"10.3389\/fpls.2022.830224","article-title":"Nitrogen source matters: High NH4\/NO3 ratio reduces cannabinoids, terpenoids, and yield in medical cannabis","volume":"13","author":"Saloner","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"116969","DOI":"10.1016\/j.indcrop.2023.116969","article-title":"Nitrogen deficiency stimulates cannabinoid biosynthesis in medical cannabis plants by inducing a metabolic shift towards production of low-N metabolites","volume":"202","author":"Song","year":"2023","journal-title":"Ind. Crop. Prod."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Westmoreland, F.M., and Bugbee, B. (2022). Sustainable Cannabis Nutrition: Elevated root-zone phosphorus significantly increases leachate P and does not improve yield or quality. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1015652"},{"key":"ref_43","first-page":"1","article-title":"Visual symptoms of plant nutrient deficiencies in nursery and landscape plants","volume":"10","author":"Wong","year":"2005","journal-title":"Soil Crop Manag."},{"key":"ref_44","first-page":"1","article-title":"Plant nutrient functions and deficiency and toxicity symptoms","volume":"9","author":"McCauley","year":"2011","journal-title":"Nutr. Manag. Modul."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fpls.2019.01727","article-title":"Magnesium fertilization improves crop yield in most production systems: A meta-analysis","volume":"10","author":"Wang","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11104-016-2871-8","article-title":"Magnesium applications to growth medium and foliage affect the starch distribution, increase the grain size and improve the seed germination in wheat","volume":"406","author":"Ceylan","year":"2016","journal-title":"Plant Soil"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11104-021-05139-w","article-title":"Physiological and molecular advances in magnesium nutrition of plants","volume":"468","author":"Tian","year":"2021","journal-title":"Plant Soil"},{"key":"ref_48","first-page":"23","article-title":"Magnesium: A forgotten element in crop production","volume":"94","author":"Cakmak","year":"2010","journal-title":"Better Crop."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s11104-012-1555-2","article-title":"The significance of magnesium for crop quality","volume":"368","year":"2013","journal-title":"Plant Soil"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"766","DOI":"10.3389\/fpls.2019.00766","article-title":"Critical leaf magnesium thresholds and the impact of magnesium on plant growth and photo-oxidative defense: A systematic review and meta-analysis from 70 years of research","volume":"10","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1007\/s12011-010-8830-y","article-title":"Cerium relieves the inhibition of chlorophyll biosynthesis of maize caused by magnesium deficiency","volume":"143","author":"Zhou","year":"2011","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1046\/j.1469-8137.2000.00616.x","article-title":"Physiological impacts of Mg deficiency in Pinus radiata: Growth and photosynthesis","volume":"146","author":"Laing","year":"2000","journal-title":"New Phytol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.scienta.2005.12.006","article-title":"Magnesium deficiency induced oxidative stress and antioxidant responses in mulberry plants","volume":"108","author":"Tewari","year":"2006","journal-title":"Sci. Hortic."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s11104-014-2150-5","article-title":"Physiological and biochemical impacts of magnesium-deficiency in two cultivars of coffee","volume":"382","author":"Alves","year":"2014","journal-title":"Plant Soil"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nplants.2017.20","article-title":"Surface charge dynamics in photosynthetic membranes and the structural consequences","volume":"3","author":"Puthiyaveetil","year":"2017","journal-title":"Nat. Plants"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s11120-008-9291-4","article-title":"Recent overview of the Mg branch of the tetrapyrrole biosynthesis leading to chlorophylls","volume":"96","author":"Masuda","year":"2008","journal-title":"Photosynth. Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1023\/A:1022458108678","article-title":"Rubisco Activase\u2014Rubisco\u2019s catalytic chaperone","volume":"75","author":"Portis","year":"2003","journal-title":"Photosynth. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s12011-009-8392-z","article-title":"Cerium relieves the inhibition of nitrogen metabolism of spinach caused by magnesium deficiency","volume":"132","author":"Yin","year":"2009","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s004680100111","article-title":"Growth, Mg nutrition and photosynthetic activity in Pinus radiata: Evidence that NaCl addition counteracts the impact of low Mg supply","volume":"15","author":"Sun","year":"2001","journal-title":"Trees"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1002\/jpln.201100329","article-title":"Magnesium deficiency\u2013induced impairment of photosynthesis in leaves of fruiting Citrus reticulata trees accompanied by up-regulation of antioxidant metabolism to avoid photo-oxidative damage","volume":"175","author":"Tang","year":"2012","journal-title":"J. Plant Nutr. Soil Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3389\/fpls.2014.00781","article-title":"Photosynthetic capacity, nutrient status, and growth of maize (Zea mays L.) upon MgSO4 leaf-application","volume":"5","author":"Jezek","year":"2015","journal-title":"Front. Plant Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1093\/jexbot\/51.suppl_1.407","article-title":"Photosynthesis, sugars and the regulation of gene expression","volume":"51","author":"Pego","year":"2000","journal-title":"J. Exp. Bot."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s00425-004-1376-5","article-title":"Magnesium deficiency in sugar beets alters sugar partitioning and phloem loading in young mature leaves","volume":"220","author":"Hermans","year":"2005","journal-title":"Planta"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1093\/jxb\/45.9.1245","article-title":"Partitioning of shoot and root dry natter and carbohydrates in bean plants suffering from phosphorus, potassium and magnesium deficiency","volume":"45","author":"Cakmak","year":"1994","journal-title":"J. Exp. Bot."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1093\/mp\/ssr014","article-title":"Membrane-transport systems for sucrose in relation to whole-plant carbon partitioning","volume":"4","author":"Ayre","year":"2011","journal-title":"Mol. Plant"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1007\/s10534-009-9246-z","article-title":"Influences of magnesium deficiency and cerium on antioxidant system of spinach chloroplasts","volume":"22","author":"Ze","year":"2009","journal-title":"BioMetals"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"756","DOI":"10.3390\/plants4040756","article-title":"Leaf senescence by magnesium deficiency","volume":"4","author":"Tanoi","year":"2015","journal-title":"Plants"},{"key":"ref_68","unstructured":"Bergmann, W. (1988). Ernaehrungsstoerungen Bei Kulturpflanzen; Entstehung und Diagnose, The Food and Agriculture Organization (FAO)."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1111\/j.1399-3054.2012.12003.x","article-title":"Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis","volume":"148","author":"Kobayashi","year":"2013","journal-title":"Physiol. Plant."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"23076","DOI":"10.3390\/ijms160923076","article-title":"Critical issues in the study of magnesium transport systems and magnesium deficiency symptoms in plants","volume":"16","author":"Kobayashi","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/35021067","article-title":"Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells","volume":"406","author":"Pel","year":"2000","journal-title":"Nature"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1111\/nph.18410","article-title":"A tonoplast-localized magnesium transporter is crucial for stomatal opening in arabidopsis under high Mg2+ Conditions","volume":"236","author":"Inoue","year":"2022","journal-title":"New Phytol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1034\/j.1399-3054.1998.1020103.x","article-title":"Magnesium deficiency results in accumulation of carbohydrates and amino acids in source and sink leaves of spinach","volume":"102","author":"Fischer","year":"1998","journal-title":"Physiol. Plant."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1071\/FP17241","article-title":"Structural and functional disorder in the photosynthetic apparatus of radish plants under magnesium deficiency","volume":"45","author":"Samborska","year":"2018","journal-title":"Funct. Plant Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1007\/s00425-004-1340-4","article-title":"Physiological characterisation of magnesium deficiency in sugar beet: Acclimation to low magnesium differentially affects photosystems I and II","volume":"220","author":"Hermans","year":"2004","journal-title":"Planta"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1007\/s00468-012-0699-2","article-title":"Physiological impacts of magnesium-deficiency in citrus seedlings: Photosynthesis, antioxidant system and carbohydrates","volume":"26","author":"Yang","year":"2012","journal-title":"Trees"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3362","DOI":"10.1021\/acs.jproteome.5b00399","article-title":"Protein dynamics in young maize root hairs in response to macro- and micronutrient deprivation","volume":"14","author":"Li","year":"2015","journal-title":"J. Proteome Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"601","DOI":"10.4236\/ajps.2011.24071","article-title":"Low magnesium with high potassium supply changes sugar partitioning and root growth pattern prior to visible magnesium deficiency in leaves of rice (Oryza sativa L.)","volume":"2","author":"Ding","year":"2011","journal-title":"Am. J. Plant Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s11104-014-2197-3","article-title":"Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using 28 Mg as a tracer","volume":"384","author":"Tanoi","year":"2014","journal-title":"Plant Soil"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s00425-018-2936-4","article-title":"Magnesium uptake characteristics in arabidopsis revealed by 28Mg tracer studies","volume":"248","author":"Ogura","year":"2018","journal-title":"Planta"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"022168","DOI":"10.1088\/1755-1315\/170\/2\/022168","article-title":"Effect of soil magnesium on plants: A review","volume":"170","author":"Yan","year":"2018","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1023\/A:1026568329860","article-title":"Effects of low and high levels of magnesium on the response of sunflower plants grown with ammonium and nitrate","volume":"225","author":"Lasa","year":"2000","journal-title":"Plant Soil"},{"key":"ref_83","first-page":"97","article-title":"Effect of magnesium nutrition of onion (Allium cepa L.). Part I. Yielding and nutrient status","volume":"19","author":"Kleiber","year":"2012","journal-title":"Ecol. Chem. Eng. S"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.1007\/s42729-021-00569-y","article-title":"Physiological response to short-term magnesium deficiency in banana cultivars","volume":"21","author":"He","year":"2021","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/S1002-0160(08)60021-1","article-title":"High potassium aggravates the oxidative stress inducedy by magnesium deflciency in rice leaves","volume":"18","author":"Ding","year":"2008","journal-title":"Pedosphere"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1626\/pps.8.38","article-title":"Effects of excess magnesium on the growth and mineral content of rice and echinochloa","volume":"8","author":"Kobayashi","year":"2005","journal-title":"Plant Prod. Sci."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1002\/jpln.19711300204","article-title":"Antagonism between potassium, calcium and magnesium in several varieties of hybrid corn","volume":"130","author":"Pathak","year":"1971","journal-title":"Zeitschrift Pflanzenern\u00e4hrung Bodenkd."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"685","DOI":"10.2136\/sssaj1985.03615995004900030032x","article-title":"Potassium-magnesium interactions affecting nutrient uptake by wheat forage","volume":"49","author":"Ohno","year":"1985","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11738-016-2165-z","article-title":"Effects of magnesium deficiency on photosynthesis and carbohydrate partitioning","volume":"38","author":"Elkhouni","year":"2016","journal-title":"Acta Physiol. Plant."},{"key":"ref_90","unstructured":"White, P.J. (2012). Marschner\u2019s Mineral Nutrition of Higher Plants, Elsevier."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1080\/01904169709365294","article-title":"Distribution pattern of dry weight, nitrogen, phosphorus, and potassium through tomato ontogenesis","volume":"20","author":"Tapia","year":"1997","journal-title":"J. Plant Nutr."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"9408","DOI":"10.1021\/jf901440n","article-title":"Impact of nitrogen and sulfur fertilization on the composition of glucosinolates in relation to sulfur assimilation in different plant organs of broccoli","volume":"57","author":"Omirou","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Yu, K.-Q., Zharo, Y.-R., Li, X.-L., Shao, Y.-N., Liu, F., and He, Y. (2014). Hyperspectral imaging for mapping of total nitrogen spatial distribution in pepper plant. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0116205"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1142\/9789814407755_0019","article-title":"Magnesium chelatase: The molecular motor of chlorophyll biosynthesis","volume":"28","author":"Hansson","year":"2013","journal-title":"Handbook of Porphyrin Science"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1023\/B:PHOT.0000046153.08935.4c","article-title":"Effects of nitrogen deficiency on gas exchange, chlorophyll fluorescence, and antioxidant enzymes in leaves of rice plants","volume":"42","author":"Huang","year":"2004","journal-title":"Photosynthetica"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s11104-019-04157-z","article-title":"Magnesium supports nitrogen uptake through regulating NRT2.1\/2.2 in soybean","volume":"457","author":"Peng","year":"2020","journal-title":"Plant Soil"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"11095","DOI":"10.1038\/ncomms11095","article-title":"Identification of plant vacuolar transporters mediating phosphate storage","volume":"7","author":"Liu","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"458","DOI":"10.2136\/sssaj1954.03615995001800040026x","article-title":"The effect of magnesium upon the growth and the phosphorus content of soybean Plants","volume":"18","author":"Webb","year":"1954","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"9012","DOI":"10.1038\/s41598-021-88588-8","article-title":"Evidence for magnesium-phosphorus synergism and Co-limitation of grain yield in wheat agriculture","volume":"11","author":"Weih","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_100","unstructured":"Broadley, M., Brown, P., Cakmak, I., Rengel, Z., and Zhao, F. (2012). Marschner\u2019s Mineral Nutrition of Higher Plants, Elsevier."},{"key":"ref_101","first-page":"332","article-title":"Role of micronutrients in vegetable production: A review","volume":"8","author":"Sharma","year":"2019","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s00425-004-1425-0","article-title":"Effects of magnesium availability on the activity of plasma membrane ion transporters and light-induced responses from broad bean leaf mesophyll","volume":"221","author":"Shabala","year":"2005","journal-title":"Planta"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/BF00009261","article-title":"The significance of the magnesium to manganese ratio in plant tissues for growth and alleviation of manganese toxicity in tomato (Lycopersicon esculentum) and wheat (Triticum aestivum) plants","volume":"124","author":"Goss","year":"1990","journal-title":"Plant Soil"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1080\/01904169609365141","article-title":"Soil pH and magnesium effects on manganese toxicity in peanuts","volume":"19","author":"Davis","year":"1996","journal-title":"J. Plant Nutr."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"588","DOI":"10.21273\/JASHS.111.4.588","article-title":"Effect of magnesium and manganese nutrition on watermelon growth and manganese toxicity","volume":"111","author":"Elamin","year":"1986","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.envexpbot.2007.11.003","article-title":"Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber","volume":"63","author":"Shi","year":"2008","journal-title":"Environ. Exp. Bot."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1093\/aob\/mci189","article-title":"Selective transport of zinc, manganese, nickel, cobalt and cadmium in the root system and transfer to the leaves in young wheat plants","volume":"96","author":"Page","year":"2005","journal-title":"Ann. Bot."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1111\/j.1469-8137.2011.03814.x","article-title":"Low magnesium status in plants enhances tolerance to cadmium exposure","volume":"192","author":"Hermans","year":"2011","journal-title":"New Phytol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.plaphy.2016.06.025","article-title":"Mg deficiency affects leaf Mg remobilization and the proteome in Brassica napus","volume":"107","author":"Billard","year":"2016","journal-title":"Plant Physiol. Biochem."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1590\/S1677-04202002000200005","article-title":"The effect of zinc supply and succinate treatment on plant growth and mineral uptake in pea plant","volume":"14","author":"Stoyanova","year":"2002","journal-title":"Brazilian J. Plant Physiol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/j.1438-8677.2008.00153.x","article-title":"Effects of zinc toxicity on sugar beet (Beta vulgaris L.) plants grown in gydroponics","volume":"11","author":"Sagardoy","year":"2009","journal-title":"Plant Biol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1080\/00103624.2017.1407429","article-title":"Effects of nutrient antagonism and synergism on yield and fertilizer use efficiency","volume":"48","author":"Rietra","year":"2017","journal-title":"Commun. Soil Sci. Plant Anal."},{"key":"ref_113","first-page":"9","article-title":"Cloning and characterization of a novel Mg\/H exchanger","volume":"23","author":"Shaul","year":"1999","journal-title":"Mol. Cell. Neurosci."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/14\/2676\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:13:44Z","timestamp":1760127224000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/14\/2676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,18]]},"references-count":113,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["plants12142676"],"URL":"https:\/\/doi.org\/10.3390\/plants12142676","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,18]]}}}