{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:03:57Z","timestamp":1790301837166,"version":"4.1.0"},"reference-count":109,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"publisher","award":["2019\/32\/T\/NZ1\/00564"],"award-info":[{"award-number":["2019\/32\/T\/NZ1\/00564"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"publisher","award":["420\/2322\/17IBE"],"award-info":[{"award-number":["420\/2322\/17IBE"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"publisher","award":["0420\/2571\/18IBE"],"award-info":[{"award-number":["0420\/2571\/18IBE"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and Higher Education","award":["2019\/32\/T\/NZ1\/00564"],"award-info":[{"award-number":["2019\/32\/T\/NZ1\/00564"]}]},{"name":"Ministry of Science and Higher Education","award":["420\/2322\/17IBE"],"award-info":[{"award-number":["420\/2322\/17IBE"]}]},{"name":"Ministry of Science and Higher Education","award":["0420\/2571\/18IBE"],"award-info":[{"award-number":["0420\/2571\/18IBE"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Nitric oxide (NO) is a signaling molecule that participates in plant adaptation to adverse environmental factors. This study aimed to clarify the role of NO in the regulation of vacuolar H+-ATPase (V-ATPase) in the roots of cucumber seedlings grown under control and Cd stress conditions. In addition, the relationship between NO and salicylic acid (SA), as well as their interrelations with hydrogen sulfide (H2S) and hydrogen peroxide (H2O2), have been verified. The effect of NO on V-ATPase was studied by analyzing two enzyme activities, the expression level of selected VHA genes and the protein level of selected VHA subunits in plants treated with a NO donor (sodium nitroprusside, SNP) and NO biosynthesis inhibitors (tungstate, WO42\u2212 and N-nitro-L-arginine methyl ester, L-NAME). Our results indicate that NO functions as a positive regulator of V-ATPase and that this regulation depends on NO generated by nitrate reductase and NOS-like activity. It was found that the mechanism of NO action is not related to changes in the gene expression or protein level of the V-ATPase subunits. The results suggest that in cucumber roots, NO signaling interacts with the SA pathway and, to a lesser extent, with two other known V-ATPase regulators, H2O2 and H2S.<\/jats:p>","DOI":"10.3390\/plants12152884","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T06:38:48Z","timestamp":1691390328000},"page":"2884","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions"],"prefix":"10.3390","volume":"12","author":[{"given":"Magdalena","family":"Zboi\u0144ska","sequence":"first","affiliation":[{"name":"Department of Plant Molecular Physiology, Faculty of Biological Sciences, University of Wroc\u0142aw, Kanonia 6\/8, 50-328 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6584-3250","authenticated-orcid":false,"given":"Anna","family":"Janeczko","sequence":"additional","affiliation":[{"name":"The Franciszek G\u00f3rski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0421-8018","authenticated-orcid":false,"given":"Katarzyna","family":"Kaba\u0142a","sequence":"additional","affiliation":[{"name":"Department of Plant Molecular Physiology, Faculty of Biological Sciences, University of Wroc\u0142aw, Kanonia 6\/8, 50-328 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121433","DOI":"10.1016\/j.envpol.2023.121433","article-title":"Toxic effects of cadmium on the physiological and biochemical attributes of plants, and Phytoremediation Strategies: A Review","volume":"325","author":"Li","year":"2023","journal-title":"Environ. 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