{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:43:24Z","timestamp":1790361804828,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Cooperation Project of the Agricultural Science and Technology Program of Zhejiang Province, China","award":["2022SNJF046"],"award-info":[{"award-number":["2022SNJF046"]}]},{"name":"Cooperation Project of the Agricultural Science and Technology Program of Zhejiang Province, China","award":["10417000121CF0401G\/004"],"award-info":[{"award-number":["10417000121CF0401G\/004"]}]},{"name":"research fund of the Zhejiang Academy of Agricultural Sciences","award":["2022SNJF046"],"award-info":[{"award-number":["2022SNJF046"]}]},{"name":"research fund of the Zhejiang Academy of Agricultural Sciences","award":["10417000121CF0401G\/004"],"award-info":[{"award-number":["10417000121CF0401G\/004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Sanghuangporus, a medicinal mushroom, has gained significant attention due to its beneficial properties. Phenolic acids are among the major bioactive compounds in Sanghuangporus, known for their antioxidant and anti-inflammatory activities. To precisely quantify the phenolic acid content, we developed a method utilizing ultra-high-performance liquid chromatography with triple quadrupole (UHPLC-QqQ). This study optimized the UHPLC-QqQ conditions to simultaneously separate and detect eight phenolic acids in Sanghuangporus baumii (Pil\u00e1t) L.W. Zhou and Y.C. Dai, including chlorogenic acid, p-coumaric acid, caffeic acid, cryptochlorogenic acid, protocatechuic acid, ferulic acid, sinapic acid, and syringic acid. The separation process utilized a ZORBAX Eclipse Plus C18 column using 0.01% formic acid and 2 mmol\/L ammonium formate in water as the aqueous phase and methanol containing 0.01% formic acid and 2 mmol\/L ammonium formate as the organic phase. Calibration curves were constructed using standard solutions to quantitatively determine the phenolic acid content. The results showed significant variation in phenolic acid content among S. baumii fruiting bodies, with Protocatechuic acid, p-coumaric acid, and caffeic acid being the most abundant. This method is valuable for quantifying phenolic acid compounds under different cultivation conditions. It provides excellent sensitivity, selectivity, and reproducibility for the quantification of phenolic acids in Sanghuangporus, contributing to a better understanding of its chemical composition and potential health benefits. This approach represents a novel technical means for the simultaneous analysis of compound phenolic acids in Sanghuangporus fruiting bodies.<\/jats:p>","DOI":"10.3390\/plants12203565","type":"journal-article","created":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T10:04:39Z","timestamp":1697191479000},"page":"3565","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of Sanghuangporus baumii (Pil\u00e1t) L.W. Zhou and Y.C. Dai"],"prefix":"10.3390","volume":"12","author":[{"given":"Zhongjing","family":"Zhou","sequence":"first","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9520-0619","authenticated-orcid":false,"given":"Shuang","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 311402, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Teng","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weike","family":"Wang","sequence":"additional","affiliation":[{"name":"Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhong","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 311402, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"129980","DOI":"10.1016\/j.foodchem.2021.129980","article-title":"Variations in phenolic acids and antioxidant activity of navel orange at different growth stages","volume":"360","author":"Hou","year":"2021","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"109298","DOI":"10.1016\/j.foodres.2020.109298","article-title":"Phenolic acids from vegetables: A review on processing stability and health benefits","volume":"136","author":"Rashmi","year":"2020","journal-title":"Food Res. 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