{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:36:33Z","timestamp":1760142993236,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T00:00:00Z","timestamp":1705622400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of South Africa","award":["117898","TTK190403426633"],"award-info":[{"award-number":["117898","TTK190403426633"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Pterocarpus anglonesis DC is an indigenous medicinal plant belonging to the Pterocarpus genus of the Fabaceae family. It is used to treat stomach problems, headaches, mouth ulcers, malaria, blackwater fever, gonorrhea, ringworm, diarrhea, heavy menstruation, and breast milk stimulation. Column chromatography of the stem bark extracts resulted in the isolation of eight compounds, which included friedelan-3-one (1), 3\u03b1-hydroxyfriedel-2-one (2), 3-hydroxyfriedel-3-en-2-one (3), lup-20(29)-en-3-ol (4), Stigmasta-5-22-dien-3-ol (5), 4-O-methylangolensis (6), (3\u03b2)-3-acetoxyolean-12-en-28-oic acid (7), and tetradecyl (E)-ferulate (8). The structures were established based on NMR, IR, and MS spectroscopic analyses. Triple-negative breast cancer (HCC70), hormone receptor-positive breast cancer (MCF-7), and non-cancerous mammary epithelial cell lines (MCF-12A) were used to test the compounds\u2019 cytotoxicity. Overall, the compounds showed either no toxicity or very low toxicity to all three cell lines tested, except for the moderate toxicity displayed by lupeol (4) towards the non-cancerous MCF-12A cells, with an IC50 value of 36.60 \u03bcM. Compound (3\u03b2)-3-acetoxyolean-12-en-28-oic acid (7) was more toxic towards hormone-responsive (MCF-7) breast cancer cells than either triple-negative breast cancer (HCC70) or non-cancerous breast epithelial (MCF-12A) cells (IC50 values of 83.06 vs. 146.80 and 143.00 \u03bcM, respectively).<\/jats:p>","DOI":"10.3390\/plants13020301","type":"journal-article","created":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T07:00:32Z","timestamp":1705647632000},"page":"301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phytochemicals from Pterocarpus angolensis DC and Their Cytotoxic Activities against Breast Cancer Cells"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1391-4878","authenticated-orcid":false,"given":"Zecarias W.","family":"Teclegeorgish","sequence":"first","affiliation":[{"name":"Department of Chemistry, Tshwane University of Technology, Private Bag X680, Pretoria 0083, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ntebogeng S.","family":"Mokgalaka","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tshwane University of Technology, Private Bag X680, Pretoria 0083, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3771-128X","authenticated-orcid":false,"given":"Douglas","family":"Kemboi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tshwane University of Technology, Private Bag X680, Pretoria 0083, South Africa"},{"name":"Department of Chemistry, University of Kabianga, Kericho 2030-20200, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6788-6449","authenticated-orcid":false,"given":"Rui W. M.","family":"Krause","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Rhodes University, Makhanda 6140, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8667-8351","authenticated-orcid":false,"given":"Xavier","family":"Siwe-Noundou","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Science, Sefako Makgatho Health Science University, P.O. Box 60, Medunsa, Pretoria 0204, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Getrude R.","family":"Nyemba","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, Female Cancers Research at Rhodes University (FemCR2U), Makhanda 6140, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Candace","family":"Davison","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, Female Cancers Research at Rhodes University (FemCR2U), Makhanda 6140, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo-Anne","family":"de la Mare","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, Female Cancers Research at Rhodes University (FemCR2U), Makhanda 6140, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vuyelwa J.","family":"Tembu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tshwane University of Technology, Private Bag X680, Pretoria 0083, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/s41597-021-00997-6","article-title":"The World Checklist of Vascular Plants is a continuously updated resource for exploring global plant diversity","volume":"8","author":"Govaerts","year":"2021","journal-title":"Sci. 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