{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:15:48Z","timestamp":1787314548741,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of the Environment of the Czech Republic","award":["GFD_PdF_2023_06"],"award-info":[{"award-number":["GFD_PdF_2023_06"]}]},{"name":"Ministry of the Environment of the Czech Republic","award":["VaV_PdF_2022_02"],"award-info":[{"award-number":["VaV_PdF_2022_02"]}]},{"name":"Ministry of the Environment of the Czech Republic","award":["PrF-2024-001"],"award-info":[{"award-number":["PrF-2024-001"]}]},{"name":"Internal Fund of Faculty of Education UP","award":["GFD_PdF_2023_06"],"award-info":[{"award-number":["GFD_PdF_2023_06"]}]},{"name":"Internal Fund of Faculty of Education UP","award":["VaV_PdF_2022_02"],"award-info":[{"award-number":["VaV_PdF_2022_02"]}]},{"name":"Internal Fund of Faculty of Education UP","award":["PrF-2024-001"],"award-info":[{"award-number":["PrF-2024-001"]}]},{"name":"Internal Fund of Faculty of Science UP","award":["GFD_PdF_2023_06"],"award-info":[{"award-number":["GFD_PdF_2023_06"]}]},{"name":"Internal Fund of Faculty of Science UP","award":["VaV_PdF_2022_02"],"award-info":[{"award-number":["VaV_PdF_2022_02"]}]},{"name":"Internal Fund of Faculty of Science UP","award":["PrF-2024-001"],"award-info":[{"award-number":["PrF-2024-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The genus Salix, comprising some 400\u2013500 species, is important in various alluvial or wet habitats of the northern hemisphere. It is a promising crop for applications such as biomass production, biofuels, or environmental projects. Clear species delimitation is crucial in ecology, biotechnology, and horticulture. DArTseq markers, a genome-wide technique, were tested for species and hybrid identification. A total of 179 willow samples were analysed, including six species of Salix subgen. Salix and four species of Salix subgen. Vetrix, including those used in biomass crop production, representing important European taxa. Identification of species-specific markers, clustering analyses (principal coordinate analysis, neighbor-joining) and Bayesian methods (Structure) unambiguously identified putative hybrids. In addition to demonstrating the high efficiency of DArT-seq markers in identifying willow hybrids, we also opened-up new questions about hybridisation processes and systematics. We detected unidirectional hybridisation between S. alba and S. fragilis, forming backcross hybrids, and we rejected the hypothesis that S. fragilis does not occur naturally in Europe. Further, the isolated position of Salix triandra within the genus was confirmed.<\/jats:p>","DOI":"10.3390\/plants13050639","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T08:59:01Z","timestamp":1708937941000},"page":"639","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Detection of Hybrids in Willows (Salix, Salicaceae) Using Genome-Wide DArTseq Markers"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6882-5509","authenticated-orcid":false,"given":"Radim J.","family":"Va\u0161ut","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Education, Palacky University Olomouc, 779 00 Olomouc, Czech Republic"},{"name":"Department of Botany, Faculty of Science, Palacky University Olomouc, 783 71 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark\u00e9ta","family":"Posp\u00ed\u0161kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Phytoenergy, Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Public Research Institute, 252 43 Pr\u016fhonice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Lukavsk\u00fd","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science, Palacky University Olomouc, 783 71 Olomouc, Czech Republic"},{"name":"The Nature Conservation Agency of the Czech Republic, Moravian-Silesian Regional Branch, 756 61 Ro\u017enov pod Radho\u0161t\u011bm, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Weger","sequence":"additional","affiliation":[{"name":"Department of Phytoenergy, Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Public Research Institute, 252 43 Pr\u016fhonice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"key":"ref_1","first-page":"69","article-title":"Hybrid Zones: Windows on Evolutionary Process","volume":"Volume 7","author":"Harrison","year":"1991","journal-title":"Oxford Surveys in Evolutionary Biology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1111\/j.1469-8137.2008.02396.x","article-title":"Yield and spatial supply of bioenergy poplar and willow short-rotation coppice in the UK","volume":"178","author":"Aylott","year":"2008","journal-title":"New Phytol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/fes3.82","article-title":"Advances in shrub-willow crops for bioenergy, renewable products, and environmental benefits","volume":"5","author":"Volk","year":"2016","journal-title":"Food Energy Secur."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1111\/gcbb.12498","article-title":"Poplar and shrub willow energy crops in the United States: Field trial results from the multiyear regional feedstock partnership and yield potential maps based on the PRISM-ELM Model","volume":"10","author":"Volk","year":"2018","journal-title":"GCB Bioenergy"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s11270-005-6272-5","article-title":"Willows beyond wetlands: Uses of Salix L. species for environmental projects","volume":"162","author":"Kuzovkina","year":"2005","journal-title":"Water Air Soil Pollut."},{"key":"ref_6","unstructured":"DAISIE (2009). 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