{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:30:54Z","timestamp":1790645454424,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Winall Hi-tech Seed Co., Ltd.","award":["GMLM2023"],"award-info":[{"award-number":["GMLM2023"]}]},{"name":"Winall Hi-tech Seed Co., Ltd.","award":["2022B02052-2"],"award-info":[{"award-number":["2022B02052-2"]}]},{"name":"Winall Hi-tech Seed Co., Ltd.","award":["31771851"],"award-info":[{"award-number":["31771851"]}]},{"name":"Xinjiang Uygur Autonomous Region Key Research and Development Task and Special Project","award":["GMLM2023"],"award-info":[{"award-number":["GMLM2023"]}]},{"name":"Xinjiang Uygur Autonomous Region Key Research and Development Task and Special Project","award":["2022B02052-2"],"award-info":[{"award-number":["2022B02052-2"]}]},{"name":"Xinjiang Uygur Autonomous Region Key Research and Development Task and Special Project","award":["31771851"],"award-info":[{"award-number":["31771851"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["GMLM2023"],"award-info":[{"award-number":["GMLM2023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022B02052-2"],"award-info":[{"award-number":["2022B02052-2"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771851"],"award-info":[{"award-number":["31771851"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Cold shock proteins (CSPs) are DNA\/RNA binding proteins with crucial regulatory roles in plant growth, development, and stress responses. In this study, we employed bioinformatics tools to identify and analyze the physicochemical properties, conserved domains, gene structure, phylogenetic relationships, cis-acting elements, subcellular localization, and expression patterns of the cotton CSP gene family. A total of 62 CSP proteins were identified across four cotton varieties (Gossypium arboreum, Gossypium raimondii, Gossypium barbadense, Gossypium hirsutum) and five plant varieties (Arabidopsis thaliana, Brassica chinensis, Camellia sinensis, Triticum aestivum, and Oryza sativa). Phylogenetic analysis categorized cotton CSP proteins into three evolutionary branches, revealing similar gene structures and motif distributions within each branch. Analysis of gene structural domains highlighted the conserved CSD and CCHC domains across all cotton CSP families. Subcellular localization predictions indicated predominant nuclear localization for CSPs. Examination of cis-elements in gene promoters revealed a variety of elements responsive to growth, development, light response, hormones, and abiotic stresses, suggesting the potential regulation of the cotton CSP family by different hormones and their involvement in diverse stress responses. RT-qPCR results suggested that GhCSP.A1, GhCSP.A2, GhCSP.A3, and GhCSP.A7 may play roles in cotton\u2019s response to low-temperature stress. In conclusion, our findings underscore the significant role of the CSP gene family in cotton\u2019s response to low-temperature stress, providing a foundational basis for further investigations into the functional aspects and molecular mechanisms of cotton\u2019s response to low temperatures.<\/jats:p>","DOI":"10.3390\/plants13050643","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T08:59:01Z","timestamp":1708937941000},"page":"643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Cold Shock Protein (CSP) Gene Family in Cotton Hypothermia Stress"],"prefix":"10.3390","volume":"13","author":[{"given":"Yejun","family":"Yang","sequence":"first","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Jinzhong 030800, China"},{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Jinzhong 030800, China"},{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianglin","family":"Xu","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanchun","family":"Pu","sequence":"additional","affiliation":[{"name":"Institute of Western Agriculture, The Chinese Academy of Agricultural Sciences, Changji 831100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfang","family":"Qu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Jinzhong 030800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Sun","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"35248","DOI":"10.1074\/jbc.M205774200","article-title":"A cold-regulated nucleic acid-binding protein of winter wheat shares a domain with bacterial cold shock proteins","volume":"277","author":"Karlson","year":"2002","journal-title":"J. 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