{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:49:18Z","timestamp":1788245358897,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-16-00047"],"award-info":[{"award-number":["21-16-00047"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Many bacterial plant pathogens have a broad host range important for their life cycle. Alternate hosts from plant families other than the main (primary) host support the survival and dissemination of the pathogen population even in absence of main host plants. Metabolic peculiarities of main and alternative host plants can affect genetic diversity within and between the pathogen populations isolated from those plants. Strains of Gram-positive bacterium Curtobacterium flaccumfaciens were identified as being causal agents of bacterial spot and wilt diseases on leguminous plants, and other crop and weed plants, collected in different regions of Russia. Their biochemical properties and susceptibility to copper compounds have been found to be relatively uniform. According to conventional PCR assays, all of the isolates studied were categorised as pathovar Curtobacterim flaccumfaciens pv. flaccumfaciens, a pathogen of legumes. However, the strains demonstrated a substantial diversity in terms of virulence on several tested host plants and different phylogenetic relationships were revealed by BOX-PCR and alanine synthase gene (alaS) sequencing.<\/jats:p>","DOI":"10.3390\/plants13050667","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T06:14:22Z","timestamp":1709100862000},"page":"667","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Phytopathogenic Curtobacterium flaccumfaciens Strains Circulating on Leguminous Plants, Alternative Hosts and Weeds in Russia"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9626-3416","authenticated-orcid":false,"given":"Anna D.","family":"Tokmakova","sequence":"first","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16\/10, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3235-8467","authenticated-orcid":false,"given":"Rashit I.","family":"Tarakanov","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Russian State Agrarian University\u2014Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, Moscow 127434, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8069-7087","authenticated-orcid":false,"given":"Anna A.","family":"Lukianova","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16\/10, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1646-9802","authenticated-orcid":false,"given":"Peter V.","family":"Evseev","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16\/10, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9486-3389","authenticated-orcid":false,"given":"Lyubov V.","family":"Dorofeeva","sequence":"additional","affiliation":[{"name":"All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Prosp. Nauki 5, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2948-753X","authenticated-orcid":false,"given":"Alexander N.","family":"Ignatov","sequence":"additional","affiliation":[{"name":"Agrobiotechnology Department, Agrarian and Technological Institute, RUDN University, Miklukho-Maklaya Str., 6, Moscow 117198, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5014-8375","authenticated-orcid":false,"given":"Fevzi S.-U.","family":"Dzhalilov","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Russian State Agrarian University\u2014Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, Moscow 127434, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6648-5889","authenticated-orcid":false,"given":"Sergei A.","family":"Subbotin","sequence":"additional","affiliation":[{"name":"Center of Parasitology, Severtsov Institute of Ecology and Evolution, Leninsky Prosp., 33, Moscow 119071, Russia"},{"name":"California Department of Food and Agriculture, 1220 N. Str., Sacramento, CA 95832, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4468-4091","authenticated-orcid":false,"given":"Konstantin A.","family":"Miroshnikov","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16\/10, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"ref_1","unstructured":"FAO (2023). World Food and Agriculture\u2014Statistical Yearbook 2023, FAO."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1080\/07352689.2014.898445","article-title":"Achievements and Challenges in Legume Breeding for Pest and Disease Resistance","volume":"34","author":"Rubiales","year":"2015","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Dell\u2019Olmo, E., Tiberini, A., and Sigillo, L. (2023). Leguminous Seedborne Pathogens: Seed Health and Sustainable Crop Management. Plants, 12.","DOI":"10.3390\/plants12102040"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3545","DOI":"10.1099\/00221287-129-11-3545","article-title":"Reclassification of Corynebacterium flaccumfaciens, Corynebacterium betae, Corynebacterium oortii and Corynebacterium poinsettiae in the Genus Curtobacterium, as Curtobacterium flaccumfaciens Comb. Nov","volume":"129","author":"Collins","year":"1983","journal-title":"Microbiology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1111\/mpp.12926","article-title":"Bacterial Wilt of Dry Beans Caused by Curtobacterium flaccumfaciens pv. flaccumfaciens: A New Threat from an Old Enemy","volume":"21","author":"Osdaghi","year":"2020","journal-title":"Mol. Plant Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1126\/science.55.1425.433","article-title":"A Bacterial Wilt of the Bean Caused by Bacterium flaccumfaciens Nov. Sp.","volume":"55","author":"Hedges","year":"1922","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1094\/PDIS-03-15-0299-FE","article-title":"Bacterial Wilt of Dry-Edible Beans in the Central High Plains of the U.S.: Past, Present, and Future","volume":"99","author":"Harveson","year":"2015","journal-title":"Plant Dis."},{"key":"ref_8","unstructured":"Allen, D.J. (1995). An Annotated List of Diseases, Pathogens and Associated Fungi of the Common Bean (Phaseolus vulgaris) in Eastern and Southern Africa, CAB International."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1094\/PDIS-08-22-1778-PDN","article-title":"First Report of Curtobacterium flaccumfaciens pv. flaccumfaciens Causing Bacterial Tan Spot of Soybean in Russia","volume":"107","author":"Tarakanov","year":"2023","journal-title":"Plant Dis."},{"key":"ref_10","first-page":"13","article-title":"Bacterial diseases of soybean in the Soviet Far East and in southern regions of the USSR: Search for sources of resistance to them","volume":"62","author":"Nikitina","year":"1978","journal-title":"Tr. Prikl. Bot. Genet. Sel."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1094\/PDIS-05-22-1203-PDN","article-title":"First Report of Curtobacterium flaccumfaciens pv. flaccumfaciens Causing Bacterial Wilt and Blight on Sunflower in Russia","volume":"107","author":"Pilik","year":"2023","journal-title":"Plant Dis."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Plant Health (EFSA PLH Panel), Jeger, M., Bragard, C., Caffier, D., Candresse, T., Chatzivassiliou, E., Dehnen-Schmutz, K., Gilioli, G., Gr\u00e9goire, J.-C., and Jaques Miret, J.A. (2018). Pest Categorisation of Curtobacterium flaccumfaciens pv. flaccumfaciens. EFSA J., 16, e05299.","DOI":"10.2903\/j.efsa.2018.5299"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"71","DOI":"10.15407\/microbiolj78.06.071","article-title":"Phytopathogenic Bacteria in Contemporary Agriculture","volume":"78","author":"Patyka","year":"2016","journal-title":"Mikrobiol. Z."},{"key":"ref_14","first-page":"22","article-title":"Curtobacterium flaccumfaciens pv. flaccumfaciens is the agent of bacterial disease of soybean","volume":"75","author":"Gnatiuk","year":"2013","journal-title":"Mikrobiol. Z."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1094\/PDIS-05-15-0535-PDN","article-title":"First Report of Bacterial Leaf Spot Caused by Curtobacterium flaccumfaciens on Dendrobium officinale Kimura et Migo in China","volume":"100","author":"Wang","year":"2016","journal-title":"Plant Dis."},{"key":"ref_16","first-page":"1639","article-title":"Correlation of bacterial diversity in rot Chinese cabbage with the habitat","volume":"51","author":"Du","year":"2011","journal-title":"Wei Sheng Wu Xue Bao"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1111\/ppa.13206","article-title":"Survival of Curtobacterium flaccumfaciens pv. flaccumfaciens in Weeds","volume":"69","author":"Nascimento","year":"2020","journal-title":"Plant Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"889","DOI":"10.3390\/cimb44020060","article-title":"Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics","volume":"44","author":"Evseev","year":"2022","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1046\/j.1472-765X.2002.01187.x","article-title":"PCR-based Assay for the Detection of Curtobacterium flaccumfaciens pv. flaccumfaciens in Bean Seeds","volume":"35","author":"Tegli","year":"2002","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"EPPO (2010). OEPP\/EPPO PM 7\/100(1): Rep-PCR Tests for Identification of Bacteria. Bulletin OEPP\/EPPO, 40, 365\u2013368.","DOI":"10.1111\/j.1365-2338.2010.02409.x"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2286","DOI":"10.1128\/aem.60.7.2286-2295.1994","article-title":"Specific Genomic Fingerprints of Phytopathogenic Xanthomonas and Pseudomonas Pathovars and Strains Generated with Repetitive Sequences and PCR","volume":"60","author":"Louws","year":"1994","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Marques, A., Marchaison, A., Gardan, L., and Samson, R. (2008). BOX-PCR-Based Identification of Bacterial Species Belonging to Pseudomonas Syringae-P. Viridiflava Group. Genet. Mol. Biol., 31.","DOI":"10.1590\/S1415-47572008000100019"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chase, A.B., Arevalo, P., Polz, M.F., Berlemont, R., and Martiny, J.B.H. (2016). Evidence for Ecological Flexibility in the Cosmopolitan Genus Curtobacterium. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01874"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s10658-018-01648-0","article-title":"Genetic Diversity of Curtobacterium flaccumfaciens Revealed by Multilocus Sequence Analysis","volume":"154","author":"Soman","year":"2019","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1007\/s12275-018-8014-6","article-title":"UBCG: Up-to-Date Bacterial Core Gene Set and Pipeline for Phylogenomic Tree Reconstruction","volume":"56","author":"Na","year":"2018","journal-title":"J. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s10658-016-1094-4","article-title":"Alternative Hosts of Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bean Bacterial Wilt","volume":"148","author":"Schipanski","year":"2017","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1111\/ppa.12730","article-title":"Epiphytic Curtobacterium flaccumfaciens Strains Isolated from Symptomless Solanaceous Vegetables Are Pathogenic on Leguminous but Not on Solanaceous Plants","volume":"67","author":"Osdaghi","year":"2018","journal-title":"Plant Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1590\/S0100-204X2003000600015","article-title":"de Rea\u00e7\u00e3o de cultivares de soja a isolado de Curtobacterium flaccumfaciens pv. flaccumfaciens, proveniente de feijoeiro","volume":"38","author":"Maringoni","year":"2003","journal-title":"Pesq. Agropec. Bras."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1590\/S0100-41582007000500011","article-title":"Tratamentos t\u00e9rmico e qu\u00edmico de sementes de feijoeiro: Efici\u00eancia na erradica\u00e7\u00e3o de Curtobacterium flaccumfaciens pv. flaccumfaciens e efeitos na qualidade fisiol\u00f3gica das sementes","volume":"32","author":"Estefani","year":"2007","journal-title":"Fitopatol. Bras."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"147","DOI":"10.3186\/jjphytopath.60.147","article-title":"Occurrence and Properties of Copper-Resistance in Plant Pathogenic Bacteria","volume":"60","author":"Goto","year":"1994","journal-title":"Jpn. J. Phytopathol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Fatmi, M., Walcott, R.R., and Schaad, N.W. (2017). Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material, APS Publications.","DOI":"10.1094\/9780890545416"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"117","DOI":"10.15258\/sst.2006.34.1.12","article-title":"Semi-Selective Culture Medium for Curtobacterium flaccumfaciens pv. flaccumfaciens Isolation from Bean Seeds","volume":"34","author":"Maringoni","year":"2006","journal-title":"Seed Sci. Technol."},{"key":"ref_33","first-page":"63","article-title":"Studi Sulla Diagnosi Di Curtobacterium flaccumfaciens pv. flaccumfaciens Nei Semi Di Fagiolo","volume":"9","author":"Tegli","year":"1998","journal-title":"Not. Sulla Prot. Delle Piante"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Pavel, A.B., and Vasile, C.I. (2012). PyElph\u2014A Software Tool for Gel Images Analysis and Phylogenetics. BMC Bioinform., 13.","DOI":"10.1186\/1471-2105-13-9"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies","volume":"32","author":"Nguyen","year":"2015","journal-title":"Mol. Biol. Evol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (iTOL) v5: An Online Tool for Phylogenetic Tree Display and Annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1099\/ijsem.0.002681","article-title":"Rathayibacter oskolensis Sp. Nov., a Novel Actinobacterium from Androsace koso-poljanskii Ovcz. (Primulaceae) Endemic to the Central Russian Upland","volume":"68","author":"Dorofeeva","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_39","first-page":"1523","article-title":"Synthetic and Complex Media for the Rapid Detection of Fluorescence of Phytopathogenic Pseudomonads: Effect of the Carbon Source","volume":"59","author":"Vidaver","year":"1967","journal-title":"Plant Pathol."},{"key":"ref_40","first-page":"1","article-title":"Gelatin Hydrolysis Test Protocol","volume":"1","author":"Torres","year":"2012","journal-title":"Am. Soc. Microbiol."},{"key":"ref_41","first-page":"370","article-title":"A Rapid Indoor Technique for Screening Common Bean (Phaseolus vulgaris L.) for Resistance to Bacterial Wilt [Curtobacterium flaccumfaciens pv. flaccumfaciens (Hedges) Collins and Jones]","volume":"21","author":"Hsieh","year":"2003","journal-title":"Rev. Mex. Fitopatol."},{"key":"ref_42","first-page":"321","article-title":"A Simple Inoculation Technique for Evaluation of Cotton Genotypes for Resistance to Bacterial Blight Caused by Xanthomonas axonopodis pv. malvacearum","volume":"112","author":"Eddin","year":"2005","journal-title":"J. Plant Dis. Prot."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Puia, J.D., Murari, R.R., Borsato, L.C., Sugahara, V.H., da Silva Machineski, G., Canteri, M.G., and Vigo, S.C. (2021). Protocol for Detecting Curtobacterium flaccumfaciens pv. flaccumfaciens in the Leaves of Infected Bean Plants (Phaseolus vulgaris L.). Acta Sci. Agron., 43.","DOI":"10.4025\/actasciagron.v43i1.51031"},{"key":"ref_44","unstructured":"CLSI (2015). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, Clinical and Laboratory Standards Institute. [10th ed.]. Approved Standard."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/13\/5\/667\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:06:20Z","timestamp":1760105180000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/13\/5\/667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,28]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["plants13050667"],"URL":"https:\/\/doi.org\/10.3390\/plants13050667","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,28]]}}}