{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:23:51Z","timestamp":1792445031613,"version":"4.5.0"},"reference-count":83,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PID2022-141721OB-C2"],"award-info":[{"award-number":["PID2022-141721OB-C2"]}]},{"name":"ERDF A way of making Europe","award":["PID2022-141721OB-C2"],"award-info":[{"award-number":["PID2022-141721OB-C2"]}]},{"name":"European Union","award":["PID2022-141721OB-C2"],"award-info":[{"award-number":["PID2022-141721OB-C2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Under iron (Fe)-limited conditions, plants have developed strategies for acquiring this essential micronutrient. Several Fe sources have been studied as potential fertilizers, with Fe synthetic chelates being the most used to prevent and correct Fe chlorosis in crops. The determination of the activity of the Fe chelate reductase (FCR) enzyme has long been described in the literature to understand the efficiency of Strategy I plants in acquiring Fe from fertilizers under deficient conditions. Other experiments have focused on the translocation of Fe to the plant to define the effectiveness of Fe fertilizers. Yet, both assays are relevant in knowing the capacity of a novel Fe source and other compounds alleviating Fe chlorosis in Strategy I plants. This work reviews the methodologies that are used in FCR assays to evaluate novel Fe fertilizers, including the factors modulating the results obtained for FCR assay activity, such as the Fe substrate, the Fe level during the growing period and during the FCR assay, the pH, the choice of an in vivo or in vitro method, and the plant species. A discussion of the benefits of the concurrence of FCR and Fe uptake assays is then presented alongside a proposed methodology for assessing the effectiveness of Fe fertilizers, emphasizing the importance of understanding chemical and physiological plant interactions. This methodology unifies key factors that modify FCR activity and combines these with the use of the 57Fe tracer to enhance our comprehension of the efficacy of Fe-based fertilizers\u2019 effectiveness in alleviating Fe chlorosis. This comprehensive approach not only contributes to the fundamental understanding of Fe-deficient Strategy I plants but also establishes a robust method for determining the efficiency of novel sources for correcting Fe deficiency in plants.<\/jats:p>","DOI":"10.3390\/plants13060819","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T03:46:31Z","timestamp":1710301591000},"page":"819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A Critical Review of Methodologies for Evaluating Iron Fertilizers Based on Iron Reduction and Uptake by Strategy I Plants"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9432-027X","authenticated-orcid":false,"given":"Alejandra","family":"Arcas","sequence":"first","affiliation":[{"name":"Department of Agricultural Chemistry and Food Science, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5035-0311","authenticated-orcid":false,"given":"Sandra","family":"L\u00f3pez-Rayo","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry and Food Science, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agust\u00edn","family":"G\u00e1rate","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry and Food Science, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9130-2909","authenticated-orcid":false,"given":"Juan J.","family":"Lucena","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry and Food Science, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.plantsci.2009.02.011","article-title":"Iron uptake mechanisms in plants: Functions of the FRO family of ferric reductases","volume":"176","author":"Jeong","year":"2009","journal-title":"Plant Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhang, X., Zhang, D., Sun, W., and Wang, T. (2019). The adaptive mechanism of plants to iron deficiency via iron uptake, transport, and homeostasis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20102424"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1747-0765.2005.tb00001.x","article-title":"Increasing iron availability to crops: Fertilizers, organo-fertilizers, and biological approaches","volume":"51","author":"Shenker","year":"2005","journal-title":"Soil Sci. Plant Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.envexpbot.2010.12.012","article-title":"Effects of moderate and severe iron deficiency chlorosis on fruit yield, appearance and composition in pear (Pyrus communis L.) and Peach (Prunus persica (L.) Batsch)","volume":"71","author":"Melgar","year":"2011","journal-title":"Environ. Exp. Bot."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1104\/pp.80.1.175","article-title":"Evidence for a specific uptake system for iron phytosiderophores in roots of grasses","volume":"80","author":"Marschner","year":"1986","journal-title":"Plant Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.pbi.2017.06.014","article-title":"Directing iron transport in dicots: Regulation of iron acquisition and translocation","volume":"39","author":"Jeong","year":"2017","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.freeradbiomed.2018.10.439","article-title":"Iron transport and its regulation in plants","volume":"133","author":"Kobayashi","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/s10725-023-00979-1","article-title":"Iron transport and homeostasis in plants: Current updates and applications for improving human nutrition values and sustainable agriculture","volume":"100","author":"Khan","year":"2023","journal-title":"Plant Growth Regul."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"190768","DOI":"10.3389\/fpls.2023.1190768","article-title":"Research progress on iron absorption, transport, and molecular regulation strategy in plants","volume":"14","author":"Ning","year":"2023","journal-title":"Front. Plant Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev-arplant-042811-105522","article-title":"Iron uptake, translocation, and regulation in higher plants","volume":"63","author":"Kobayashi","year":"2012","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1111\/j.1469-8137.2009.02908.x","article-title":"Dissecting iron deficiency-induced proton extrusion in Arabidopsis roots","volume":"183","author":"Santi","year":"2009","journal-title":"New Phytol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1021\/acsearthspacechem.3c00199","article-title":"Stability of coumarins and determination of the net iron oxidation state of iron\u2013coumarin complexes: Implications for examining plant iron acquisition mechanisms","volume":"7","author":"Kang","year":"2023","journal-title":"ACS Earth Space Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF00008069","article-title":"Strategies of plants for acquisition of iron","volume":"165","author":"Marschner","year":"1994","journal-title":"Plant Soil"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1469-8137.1999.00331.x","article-title":"Mechanisms and regulation of reduction-based iron uptake in plants","volume":"141","author":"Schmidt","year":"1999","journal-title":"New Phytol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1104\/pp.103.025122","article-title":"Overexpression of the FRO2 ferric chelate reductase confers tolerance to growth on low iron and uncovers posttranscriptional control","volume":"133","author":"Connolly","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"111833","DOI":"10.1016\/j.scienta.2023.111833","article-title":"Iron nutrition in agriculture: From synthetic chelates to biochelates","volume":"312","author":"Zuluaga","year":"2023","journal-title":"Sci. Hortic."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.plaphy.2016.04.050","article-title":"Effect of tris(3-hydroxy-4-pyridinonate) iron(III) complexes on iron uptake and storage in soybean (Glycine max L.)","volume":"106","author":"Santos","year":"2016","journal-title":"Plant Physiol. Biochem."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Puglisi, I., Brida, S., Stoleru, V., Torino, V., Sellitto, V.M., and Baglieri, A. (2021). Application of novel microorganism-based formulations as alternative to the use of iron chelates in strawberry cultivation. Agriculture, 11.","DOI":"10.3390\/agriculture11030217"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1080\/01904160500524886","article-title":"Synthetic iron chelates as substrates of root ferric chelate reductase in green stressed cucumber plants","volume":"29","author":"Lucena","year":"2006","journal-title":"J. Plant Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1080\/01904160701290071","article-title":"Response of cucumber plants to low doses of different synthetic iron chelates in hydroponics","volume":"30","author":"Lucena","year":"2007","journal-title":"J. Plant Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s11104-009-0115-x","article-title":"Effectiveness of N,N\u2032-Bis(2-hydroxy-5-methylbenzyl) ethylenediamine-N,N\u2032-diacetic acid (HJB) to supply iron to dicot plants","volume":"325","author":"Nadal","year":"2009","journal-title":"Plant Soil"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s11104-011-1118-y","article-title":"Fertilizer properties of DCHA\/Fe3+","volume":"356","author":"Nadal","year":"2012","journal-title":"Plant Soil"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.1039\/c2ob06754d","article-title":"Biological activity of Fe(III) aquo-complexes towards ferric chelate reductase (FCR)","volume":"10","author":"Escudero","year":"2012","journal-title":"Org. Biomol. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e00256","DOI":"10.1002\/pld3.256","article-title":"A combined physiological and biophysical approach to understand the ligand-dependent efficiency of 3-hydroxy-4-pyridinone Fe-chelates","volume":"4","author":"Santos","year":"2020","journal-title":"Plant Direct"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.plaphy.2017.07.028","article-title":"Response of soybean plants to the application of synthetic and biodegradable Fe chelates and Fe complexes","volume":"118","author":"Solti","year":"2017","journal-title":"Plant Physiol. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"65","DOI":"10.9755\/ejfa.2018.v30.i1.1586","article-title":"Azotochelin and N-dihydroxy-N,N\u2032-diisopropylhexanediamide as Fe sources to cucumber plants in hydroponic cultures","volume":"30","author":"Martins","year":"2018","journal-title":"Emir. J. Food Agric."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Di Foggia, M., Yunta-Mezquita, F., Tugnoli, V., Rombol\u00e0, A.D., and Lucena, J.J. (2020). Testing a bovine blood-derived compound as iron supply on Cucumis sativus L.. Agronomy, 10.","DOI":"10.3390\/agronomy10101480"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s11104-021-05152-z","article-title":"A synthetic phytosiderophore analog, proline-2\u2032-deoxymugineic acid, is efficiently utilized by dicots","volume":"469","author":"Ueno","year":"2021","journal-title":"Plant Soil"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"964088","DOI":"10.3389\/fpls.2022.964088","article-title":"Regreening properties of the soil slow-mobile H2bpcd\/Fe3+ complex: Steps forward to the development of a new environmentally friendly Fe fertilizer","volume":"13","author":"Piccinelli","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.plaphy.2011.01.026","article-title":"Towards a knowledge-based correction of iron chlorosis","volume":"49","year":"2011","journal-title":"Plant Physiol. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s11104-013-1842-6","article-title":"Morpho-physiological parameters affecting iron deficiency chlorosis in soybean (Glycine max L.)","volume":"374","author":"Vasconcelos","year":"2014","journal-title":"Plant Soil"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1093\/jxb\/eraa483","article-title":"Coordinated homeostasis of essential mineral nutrients: A focus on iron","volume":"72","author":"Hanikenne","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_33","unstructured":"Upadhyay, S.K. (2022). Cation Transporters in Plants, Academic Press."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Li, M., Watanabe, S., Gao, F., and Dubos, C. (2023). Iron nutrition in plants: Towards a new paradigm?. Plants, 12.","DOI":"10.3390\/plants12020384"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11104-022-05746-1","article-title":"Plant strategies to mine iron from alkaline substrates","volume":"483","author":"Schmidt","year":"2023","journal-title":"Plant Soil"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Smith, G.L., Reutovich, A.A.A., Srivastavaa, A.K., Reichard, R.E., Welsh, C.H., Melman, A., and Bou-Abdallah, F. (2021). Complexation of ferrous ions by ferrozine, 2,2\u2032-bipyridine and 1,10-phenanthroline: Implication for the quantification of iron in biological systems. J. Inor. Biochem., 220.","DOI":"10.1016\/j.jinorgbio.2021.111460"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF00024999","article-title":"Reduction of Fe(III), Mn(III), and Cu(II) chelates by roots of pea (Pisum sativum L.) or soybean (Glycine max) W.A","volume":"155","author":"Norvell","year":"1993","journal-title":"Plant Soil"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1104\/pp.50.2.208","article-title":"Obligatory reduction of ferric chelates in iron uptake by soybeans","volume":"50","author":"Chaney","year":"1972","journal-title":"Plant Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.scienta.2017.02.043","article-title":"Root plant growth promoting rhizobacteria inoculations increase ferric chelate reductase (FC-R) activity and Fe nutrition in pear under calcareous soil conditions","volume":"219","author":"Aras","year":"2017","journal-title":"Sci. Hortic."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1021\/acs.jafc.6b03274","article-title":"Effectiveness of iron ethylenediamine-N,N\u2032-bis(hydroxyphenylacetic) acid (o,o-EDDHA\/Fe3+) formulations with different ratios of meso and d,l-racemic isomers as iron fertilizers","volume":"65","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1080\/01904167.2018.1482910","article-title":"Effect of plant growth promoting rhizobacteria on Fe acquisition in peach (Prunus persica L.) under calcareous soil conditions","volume":"41","author":"Aras","year":"2018","journal-title":"J. Plant Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1007\/s11738-018-2693-9","article-title":"Plant growth promoting rhizobacteria enhanced leaf organic acids, FC-R activity and Fe nutrition of apple under lime soil conditions","volume":"40","author":"Aras","year":"2018","journal-title":"Acta Physiol. Plant."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"351","DOI":"10.3389\/fpls.2018.00351","article-title":"Complementary evaluation of iron deficiency root responses to assess the effectiveness of different iron foliar applications for chlorosis remediation","volume":"9","author":"Fuentes","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"13386","DOI":"10.1021\/acs.jafc.8b04021","article-title":"Iron and humic acid accumulation on soybean roots fertilized with leonardite iron humates under calcareous conditions","volume":"66","author":"Cieschi","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1071\/CP18371","article-title":"Effects of different foliar iron applications on activity of ferric chelate reductase and concentration of iron in sweet potato (Ipomoea batatas)","volume":"70","author":"Tan","year":"2019","journal-title":"Crop Pasture Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.plaphy.2020.07.045","article-title":"The role of plant growth-promoting rhizobacteria (PGPR) in improving iron acquisition by altering physiological and molecular responses in quince seedlings","volume":"155","author":"Rahimi","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"110285","DOI":"10.1016\/j.ecoenv.2020.110285","article-title":"Gamma-aminobutyric acid enhances tolerance to iron deficiency by stimulating auxin signaling in cucumber (Cucumis sativus L.)","volume":"192","author":"Guo","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Rahman, M.A., Parvin, M., Das, U., Ela, E.J., Lee, S.-H., Lee, K.-W., and Kabir, A.H. (2020). Arbuscular mycorrhizal symbiosis mitigates iron (Fe)-deficiency retardation in alfalfa (Medicago sativa L.) through the enhancement of Fe accumulation and sulfur-assisted antioxidant defense. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21062219"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Celletti, S., Astolfi, S., Guglielmo, N., Colla, G., Cesco, S., and Mimmo, T. (2020). Evaluation of a legume-derived protein hydrolysate to mitigate iron deficiency in plants. Agronomy, 10.","DOI":"10.3390\/agronomy10121942"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1080\/01904167.2020.1766079","article-title":"A novel plant extract as a biostimulant to recover strawberry plants from iron chlorosis","volume":"43","author":"Saavedra","year":"2020","journal-title":"J. Plant Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"110165","DOI":"10.1016\/j.scienta.2021.110165","article-title":"Arbuscular mycorrhizal fungi inoculation improves iron deficiency in quince via alterations in host root phenolic compounds and expression of genes","volume":"285","author":"Rahimi","year":"2021","journal-title":"Sci. Hortic."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"100274","DOI":"10.1016\/j.rhisph.2020.100274","article-title":"Iron metabolism in soybean grown in calcareous soil is influenced by plant growth-promoting rhizobacteria\u2014A functional analysis","volume":"17","author":"Roriz","year":"2021","journal-title":"Rhizosphere"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Xiao, J., Park, Y.G., Guo, G., and Jeong, B.R. (2021). Effect of iron source and medium pH on growth and development of Sorbus commixta in vitro. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22010133"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1007\/s00299-022-02897-4","article-title":"Brassinolide alleviates Fe deficiency-induced stress by regulating the Fe absorption mechanism in Malus hupehensis Rehd","volume":"41","author":"Sun","year":"2022","journal-title":"Plant Cell Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.plaphy.2022.08.004","article-title":"Effects of foliar application of organic acids on strawberry plants","volume":"188","author":"Saavedra","year":"2022","journal-title":"Plant Physiol. Biochem."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Singh, A., Pankaczi, F., Rana, D., May, Z., Tolnai, G., and Fodor, F. (2023). Coated hematite nanoparticles alleviate iron deficiency in cucumber in acidic nutrient solution and as foliar spray. Plants, 12.","DOI":"10.3390\/plants12173104"},{"key":"ref_57","first-page":"1035","article-title":"Iron nanoparticles for plant nutrition: Synthesis, transformation, and utilization by the roots of Cucumis sativus","volume":"38","author":"Gracheva","year":"2023","journal-title":"J. Mater. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1104\/pp.110.1.111","article-title":"The pH requirement for in vivo activity of the iron-deficiency-induced \u201cturbo\u201d ferric chelate reductase (a comparison of the iron-deficiency-induced iron reductase activities of intact plants and isolated plasma membrane fractions in sugar beet)","volume":"110","author":"Lucena","year":"1996","journal-title":"Plant Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3389\/fpls.2018.00010","article-title":"Gene expression profiling of iron deficiency chlorosis sensitive and tolerant soybean indicates key roles for phenylpropanoids under alkalinity stress","volume":"9","author":"Waters","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1080\/01904168209363012","article-title":"The chemistry of iron in soils and its availability to plants","volume":"5","author":"Lindsay","year":"1982","journal-title":"J. Plant Nutr."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.plaphy.2016.03.019","article-title":"The memory of iron stress in strawberry plants","volume":"104","author":"Gama","year":"2016","journal-title":"Plant Physiol. Biochem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1080\/01904169209364430","article-title":"Root iron-reduction capacity for genotypic evaluation of iron efficiency in soybean","volume":"15","author":"Jolley","year":"1992","journal-title":"J. Plant Nutr."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/BF00011864","article-title":"Investigations on iron uptake and reduction by excised roots of different grapevine rootstocks and a V. vinifera cultivar","volume":"130","author":"Bavaresco","year":"1991","journal-title":"Plant Soil"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1023\/A:1016083512158","article-title":"A comparison of ferric-chelate reductase and chlorophyll and growth ratios as indices of selection of quince, pear and olive genotypes under iron deficiency stress","volume":"241","year":"2002","journal-title":"Plant Soil"},{"key":"ref_65","unstructured":"Nadal, P. (2012). Interacci\u00f3n Quelato-Ra\u00edz en Los Procesos de Absorci\u00f3n de Fe, Universidad Aut\u00f3noma de Madrid."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s11104-005-8218-5","article-title":"Effectiveness of ethylenediamine-N(o-hydroxyphenylacetic)-N\u2032(p-hydroxyphenylacetic) acid (o,p-EDDHA) to supply iron to plants","volume":"279","author":"Yunta","year":"2006","journal-title":"Plant Soil"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s11104-009-0091-1","article-title":"Evaluation of 59Fe-lignosulfonates complexes as Fe-sources for plants","volume":"325","author":"Tomasi","year":"2009","journal-title":"Plant Soil"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1039\/c0mt00018c","article-title":"Using a dual-stable isotope tracer method to study the uptake, xylem transport and distribution of Fe and its chelating agent from stereoisomers of an Fe(III)-chelate used as fertilizer in Fe-deficient Strategy I Plants","volume":"2","author":"Orera","year":"2010","journal-title":"Metallomics"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"46","DOI":"10.6000\/1929-5030.2012.01.01.6","article-title":"Thermodynamic database update to model synthetic chelating agents in soil systems","volume":"1","author":"Yunta","year":"2012","journal-title":"J. Appl. Solut. Chem. Model."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"39","DOI":"10.2136\/sssaj1979.03615995004300010007x","article-title":"Effect of pH and redox on predicted heavy metal-chelate equilibria in soils","volume":"43","author":"Sommers","year":"1979","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1021\/ic00280a044","article-title":"Ferrous chelates of EDTA, hydroxyethyenediaminetriacetic acid (HEDTA) and N,N\u2032-bis (2-hydroxy-5-sulfobenzyl)ethylenediamine-N,N\u2032-diacetic acid (SHBED)","volume":"27","author":"Clark","year":"1988","journal-title":"Inorg. Chem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1080\/01904160009382160","article-title":"Remedy of chlorosis induced by iron deficiency in plants with the fungal siderophore rhizoferrin","volume":"23","author":"Yehuda","year":"2000","journal-title":"J. Plant Nutr."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/BF02990349","article-title":"A reevaluation of the Fe(II), Ca(II), Zn(II), and proton formation constants of 4,7-diphenyl-1, 10-phenanthrolinedisulfonate","volume":"30","author":"Bell","year":"1991","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1139\/B09-063","article-title":"High stability ferric chelates result in decreased iron uptake by the green alga Chlorella kessleri owing to decreased ferric reductase activity and chelation of ferrous iron","volume":"87","author":"Weger","year":"2009","journal-title":"Botany"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1438-8677.1989.tb02038.x","article-title":"Kinetics of ferric chelate reduction by roots of iron-deficient peanut (Arachis hypogeae)","volume":"38","author":"Chaney","year":"1989","journal-title":"Acta Bot. Neerl."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.chroma.2013.01.083","article-title":"Characterization and quantification of racemic and meso-ethylenediamine-N,N\u2032-bis(2-hydroxy-5-sulfophenylacetic) acid\/iron (III) by ion-pair ultra-high performance liquid chromatography coupled with diode array detector and electrospray tandem mass spectrometry","volume":"1282","author":"Biasone","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1002\/jsfa.11060","article-title":"Leonardite iron humate and synthetic iron chelate mixtures in Glycine max nutrition","volume":"101","author":"Cieschi","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1080\/01904168709363626","article-title":"Complexity of iron nutrition lessons for plant-soil interaction research","volume":"10","author":"Chaney","year":"1987","journal-title":"J. Plant Nutr."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s00216-007-1716-y","article-title":"Isotope pattern deconvolution as a tool to study iron metabolism in plants","volume":"390","author":"Moldovan","year":"2008","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.1002\/jsfa.8105","article-title":"Timing for a sustainable fertilisation of Glycine max by using HBED\/Fe3+ and EDDHA\/Fe3+ chelates","volume":"97","author":"Lucena","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1016\/j.scitotenv.2018.08.021","article-title":"[S,S]-EDDS\/Fe: A new chelate for the environmentally sustainable correction of iron chlorosis in calcareous soil","volume":"647","author":"Ferreira","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Cuenca, M.-R., Mart\u00ednez-Alc\u00e1ntara, B., Qui\u00f1ones, A., Millos, J., Legaz, F., and Qui\u00f1ones, A. (2021). Seasonal Fe uptake of young citrus trees and its contribution to the development of new organs. Plants, 10.","DOI":"10.3390\/plants10010079"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/jsfa.4197","article-title":"Use of the stable isotope 57Fe to track the efficacy of the foliar application of lignosulfonate\/Fe3+ complexes to correct Fe deficiencies in cucumber plants: Lignosulfonate\/57Fe3+ complexes to correct Fe chlorosis","volume":"91","author":"Benedicto","year":"2011","journal-title":"J. Sci. Food Agric."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/13\/6\/819\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:12:41Z","timestamp":1760105561000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/13\/6\/819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,13]]},"references-count":83,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["plants13060819"],"URL":"https:\/\/doi.org\/10.3390\/plants13060819","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,13]]}}}