{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:15:05Z","timestamp":1791317705187,"version":"4.3.1"},"reference-count":37,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:00:00Z","timestamp":1717027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hainan Province Key Research and Development Project","award":["ZDYF2023XDNY029"],"award-info":[{"award-number":["ZDYF2023XDNY029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Nymphoides coronata is an endangered aquatic plant species with significant medicinal and ecological importance. To preserve N. coronata from going extinct, we need to provide seedlings and efficient multiplication techniques so that it can be extensively studied. This study aimed to identify the most suitable sterilization treatment, growth medium, and substrate for the cultivation and propagation of N. coronata. Ethanol sterilization, fungicide treatment, and sterile water washing were the most important sterilization steps. A combination of 6-benzylaminopurine (6-BA) and indoleacetic acid (IAA) was the most suitable medium for bud induction and shoot proliferation. The use of \u03b1-naphthaleneacetic acid (NAA) increased the rooting rate and rooting time compared to indole-3-butyric acid (IBA). Increasing the concentration of NAA from 0.5 to 1.0 mg\/L increased the rooting rate from 78 to 100% and reduced the rooting time from 7 to 5 days. The survival rate of N. coronata seedlings was 100% in a mixture of red soil and sand (1:1, w\/w). As a result, the procedure mentioned above could potentially be used to safely propagate this rare species on a large scale. These findings provide valuable insights into the optimal conditions for the successful cultivation and propagation of N. coronata, which can contribute to the conservation and sustainable use of this important rare plant species.<\/jats:p>","DOI":"10.3390\/plants13111508","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T08:15:54Z","timestamp":1717056954000},"page":"1508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["In Vitro Propagation Technology for the Endangered Aquatic Species Nymphoides coronata"],"prefix":"10.3390","volume":"13","author":[{"given":"Fei","family":"Lin","sequence":"first","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, CATAS, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Haikou 571101, China"},{"name":"Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Kang","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, CATAS, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamei","family":"Li","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, CATAS, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Guo","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, CATAS, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Hainan Agriculture School, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangsui","family":"Yang","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, CATAS, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junmei","family":"Yin","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, CATAS, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Haikou 571101, China"},{"name":"National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, CATAS, Sanya 572024, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenling","family":"Tang","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, CATAS, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5308-5664","authenticated-orcid":false,"given":"Mamdouh A.","family":"Eissa","sequence":"additional","affiliation":[{"name":"Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China"},{"name":"Department of Soils and Water, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"170","DOI":"10.11646\/phytotaxa.184.3.8","article-title":"Reappraisal of Nymphoides coronata (Menyanthaceae), a 100-year-lost species endemic to South China","volume":"184","author":"Zhou","year":"2014","journal-title":"Phytotaxa"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dunn, S.T. 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