{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T15:37:51Z","timestamp":1782833871160,"version":"3.54.5"},"reference-count":19,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:00:00Z","timestamp":1718323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea, under the project titled \u201cDevelopment and application of cryopreservation protocol for pear and freesia germplasm","award":["PJ016811"],"award-info":[{"award-number":["PJ016811"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Cryopreservation is a promising method for the long-term preservation of plant germplasm, especially for vegetatively propagated species like freesias. In this study, we investigate streamlining the cryopreservation process for \u2018Sunny Gold\u2019 Freesia, starting from effective in vitro initiation and proliferation using various plant growth regulator combinations. We also assess the impact of subculture on regrowth rates after cryopreservation. The shoot tips were successfully initiated in vitro after sterilization. The shoots were multiplied an average of three times in media containing N6-benzyladenine and kinetin. The regrowth rates of non-cryopreserved shoot tips excised from different subculture cycles did not differ significantly, with rates of 44% observed for plants from more than five subcultures and 47% for those from three subcultures. However, only the shoot tips excised from cultures subjected to three subculture cycles were able to recover after cryopreservation, with a regrowth rate of 31%. Our findings lay the groundwork for the development of an efficient cryopreservation protocol for freesias in the future.<\/jats:p>","DOI":"10.3390\/plants13121655","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T10:42:34Z","timestamp":1718361754000},"page":"1655","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Micropropagation and Shoot Tip Cryopreservation of \u2018Sunny Gold\u2019 Freesia"],"prefix":"10.3390","volume":"13","author":[{"given":"Jinjoo","family":"Bae","sequence":"first","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3020-6432","authenticated-orcid":false,"given":"Jae-Young","family":"Song","sequence":"additional","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Yi","family":"Lee","sequence":"additional","affiliation":[{"name":"Planning and Coordination Division, National Institute of Agricultural Sciences, RDA, Wanju 55365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye-ji","family":"Lee","sequence":"additional","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youn Jung","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Floriculture, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oh-Keun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Floriculture, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Hee","family":"Nam","sequence":"additional","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho-sun","family":"Lee","sequence":"additional","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok Cheol","family":"Kim","sequence":"additional","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Won","family":"Han","sequence":"additional","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1515\/fhort-2015-0022","article-title":"Investigation of callogenesis and indirect regeneration of Freesia \u00d7 hybrida Bailey \u2018Argenta\u2019","volume":"27","author":"Pourkhaloee","year":"2015","journal-title":"Folia Hort."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e10742","DOI":"10.7717\/peerj.10742","article-title":"Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars","volume":"9","author":"Li","year":"2021","journal-title":"PeerJ"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"177","DOI":"10.17660\/ActaHortic.2009.813.22","article-title":"Growth rhythms of South African Iridaceae forced as pot plants","volume":"813","author":"Ehrich","year":"2009","journal-title":"Acta Hortic."},{"key":"ref_4","first-page":"97","article-title":"Pupae are excellent explants with low microbial contamination and high regeneration frequency for micropropagation of Freesia \u00d7 hybrida Bailey \u2018Argenta\u2019","volume":"2","author":"Pourkhaloee","year":"2015","journal-title":"Int. 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