{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:14:55Z","timestamp":1760145295148,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570322"],"award-info":[{"award-number":["31570322"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Regulation of microtubule dynamics is crucial during key developmental transitions such as gametogenesis, fertilization, embryogenesis, and seed formation, where cells undergo rapid changes in shape and function. In plants, katanin plays an essential role in microtubule dynamics. This study investigates two seed developmental mutants in Arabidopsis thaliana, named elk5-1D (erecta-like 5, ELK5) and loo1 (lollipop 1), which are characterized by round seeds, dwarfism, and fertility defects. Notably, elk5-1D exhibits a dominant inheritance pattern, whereas loo1 is recessive. Through positional cloning, we identified both mutants as new alleles of the KATANIN 1 (KTN1) gene, which encodes a microtubule-severing enzyme critical for cell division and morphology. Mutations in KTN1 disrupt embryo cell division and lead to the emergence of a twin embryo phenotype. Our findings underscore the essential role of KTN1 in fertility and early embryonic development, potentially influencing the fate of reproductive cells.<\/jats:p>","DOI":"10.3390\/plants13131824","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T04:23:43Z","timestamp":1719980623000},"page":"1824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Twin Embryos in Arabidopsis thaliana\u00a0KATANIN 1 Mutants"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2833-7299","authenticated-orcid":false,"given":"Youfeng","family":"Yu","sequence":"first","affiliation":[{"name":"College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Xu","sequence":"additional","affiliation":[{"name":"Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balaji","family":"Enugutti","sequence":"additional","affiliation":[{"name":"Plant Developmental Biology, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6688-0539","authenticated-orcid":false,"given":"Kay","family":"Schneitz","sequence":"additional","affiliation":[{"name":"Plant Developmental Biology, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuanpeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0989-7622","authenticated-orcid":false,"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.pbi.2019.11.003","article-title":"Twice the fun, double the trouble: Gamete interactions in flowering plants","volume":"53","author":"Sprunck","year":"2020","journal-title":"Curr. 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