{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T05:43:23Z","timestamp":1772603003489,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Programa de Apoyo a la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (PAICYT) of the Universidad Aut\u00f3noma de Nuevo Le\u00f3n","award":["CN1618-21"],"award-info":[{"award-number":["CN1618-21"]}]},{"name":"Programa de Apoyo a la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (PAICYT) of the Universidad Aut\u00f3noma de Nuevo Le\u00f3n","award":["808132"],"award-info":[{"award-number":["808132"]}]},{"name":"Consejo Nacional de Humanidades, Ciencia y Tecnolog\u00eda (CONAHCYT)","award":["CN1618-21"],"award-info":[{"award-number":["CN1618-21"]}]},{"name":"Consejo Nacional de Humanidades, Ciencia y Tecnolog\u00eda (CONAHCYT)","award":["808132"],"award-info":[{"award-number":["808132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Chagas disease, caused by the protozoan Trypanosoma cruzi, represents an important and worldwide public health issue, particularly in Latin America. Limitations of conventional treatment with benznidazole and nifurtimox underscore the urgent need for new therapeutic strategies for this disease. Schinus molle, a tree used in traditional medicine for various ailments, has demonstrated promising antiparasitic activity. The in vitro anti-T. cruzi activity of Schinus molle crude methanol extract, partitions, and fractions, as well as their cytotoxicity in Vero cells and Artemia salina, and hemolytic activity in human erythrocytes were assessed. Most of the extracts possessed anti-T. cruzi effects, with Sm-CF3 being the fraction with the highest activity (IC50 = 19 \u00b5g\/mL; SI = 6.8). Gas chromatography\u2013mass spectrometry analysis identified 20 compounds, with fatty acyls comprising the predominant chemical class (55%). We also identified the antiparasitic compounds cis-5,8,11,14,17-eicosapentaenoic acid and trans-Z-\u03b1-bisabolene epoxide, suggesting their potential contribution to the observed anti-T. cruzi activity. In conclusion, our findings support the therapeutic potential of S. molle as a source of novel antiparasitic agents against T. cruzi.<\/jats:p>","DOI":"10.3390\/plants13162177","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T11:54:19Z","timestamp":1722945259000},"page":"2177","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In Vitro Antiprotozoal Activity of Schinus molle Extract, Partitions, and Fractions against Trypanosoma cruzi"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3201-5437","authenticated-orcid":false,"given":"Nancy E.","family":"Rodr\u00edguez-Garza","sequence":"first","affiliation":[{"name":"Laboratorio de Patolog\u00eda Molecular y Experimental, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Ave. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4379-6913","authenticated-orcid":false,"given":"Ramiro","family":"Quintanilla-Licea","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Ave. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9518-4433","authenticated-orcid":false,"given":"Ricardo","family":"Gomez-Flores","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Ave. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8220-6314","authenticated-orcid":false,"given":"Lucio","family":"Galaviz-Silva","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda Molecular y Experimental, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Ave. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zinnia J.","family":"Molina-Garza","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda Molecular y Experimental, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Ave. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"ITC17","DOI":"10.7326\/AITC202302210","article-title":"Chagas Disease","volume":"176","author":"Hochberg","year":"2023","journal-title":"Ann. Intern. Med."},{"key":"ref_2","unstructured":"WHO (2024, March 08). Chagas Disease (Also Known as American Trypanosomiasis). Available online: https:\/\/www.who.int\/news-room\/questions-and-answers\/item\/chagas-disease."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1021\/acsinfecdis.2c00123","article-title":"An updated view of the Trypanosoma cruzi life cycle: Intervention points for an effective treatment","volume":"8","author":"Rosales","year":"2022","journal-title":"ACS Infect. 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