{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:37:49Z","timestamp":1760146669944,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T00:00:00Z","timestamp":1732838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Laboratory of Smart Farm Technologies and Systems","award":["JD2023GJ01-12","2016YFD0300604-4","LH2020C006"],"award-info":[{"award-number":["JD2023GJ01-12","2016YFD0300604-4","LH2020C006"]}]},{"name":"National Key Research and Development Program","award":["JD2023GJ01-12","2016YFD0300604-4","LH2020C006"],"award-info":[{"award-number":["JD2023GJ01-12","2016YFD0300604-4","LH2020C006"]}]},{"name":"Natural Science Foundation of Heilongjiang Province","award":["JD2023GJ01-12","2016YFD0300604-4","LH2020C006"],"award-info":[{"award-number":["JD2023GJ01-12","2016YFD0300604-4","LH2020C006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The number of tillers in rice significantly affects final yield, making it a key trait for breeding and nitrogen-efficient cultivation. By investigating agronomic characteristics, we analyzed phenotypic differences between the wild-type P47-1 and the mutant p47dt1, performing genetic analysis and gene mapping through population construction and BSA sequencing. The p47dt1 mutant, exhibiting dwarfism and multiple tillering, is controlled by a single gene, P47DT1, which is tightly linked to D10. A single base mutation (T to G) on chromosome 1 alters methionine to arginine, supporting D10 as the candidate gene for p47dt1. To investigate nitrogen response in tillering, KY131 (nitrogen-inefficient) and KY131OsTCP19-H (nitrogen-efficient) materials differing in TCP19 expression levels were analyzed. Promoter analysis of D10 identified TCP19 as a nitrogen-responsive transcription factor, suggesting D10\u2019s potential role in a TCP19-mediated nitrogen response pathway. Further analysis of P47-1, p47dt1, KY131, and KY131OsTCP19-H under different nitrogen concentrations revealed p47dt1\u2019s distinct tiller response to nitrogen, altered nitrogen content in stems and leaves, and changes in TCP19 expression. Additionally, D10 and TCP19 expression levels were lower in KY131OsTCP19-H than KY131 under identical conditions. In summary, P47DT1\/D10 appears to modulate nitrogen response and distribution in rice, affecting tiller response, possibly under TCP19\u2019s regulatory influence.<\/jats:p>","DOI":"10.3390\/plants13233349","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T02:57:08Z","timestamp":1732849028000},"page":"3349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Putative Allele of D10 Gene Alters Rice Tiller Response to Nitrogen"],"prefix":"10.3390","volume":"13","author":[{"given":"Tamanna Islam","family":"Rimi","sequence":"first","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"},{"name":"National Key Laboratory of Smart Farm Technologies and Systems, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meirong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"},{"name":"National Key Laboratory of Smart Farm Technologies and Systems, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruixin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyu","family":"Leng","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiafang","family":"Han","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sihan","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Du","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4913-9914","authenticated-orcid":false,"given":"Zhongchen","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China"},{"name":"National Key Laboratory of Smart Farm Technologies and Systems, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3301","DOI":"10.1093\/plcell\/koac169","article-title":"Dwarf and High Tillering1 represses rice tillering through mediating the splicing of D14 pre-mRNA","volume":"34","author":"Liu","year":"2022","journal-title":"Plant Cell"},{"key":"ref_2","first-page":"61","article-title":"Research progress of rice plant type","volume":"38","author":"Li","year":"2020","journal-title":"Nat. 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