{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:49:42Z","timestamp":1787316582800,"version":"build-2736575974"},"reference-count":45,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The present study investigates the chemical profile and biological activities of Polygonum istanbulicum M. Keskin, a species endemic to Turkey, aiming to explore its potential applications in pharmacology. We assessed its phenolic and flavonoid content by employing ethyl acetate, methanol, and water as extraction solvents. The methanol extract demonstrated the highest concentrations of these compounds, with liquid chromatography\u2013quadrupole time-of-flight tandem mass spectrometry (LC-MS-qTOF) analysis identifying a wide range of bioactive substances, such as derivatives of quercetin and myricetin. Antioxidant capacity was evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2\u2032-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), cupric-reducing antioxidant capacity (CUPRAC), ferric-reducing antioxidant power (FRAP), and phosphomolybdenum assays, with the methanol extract showing the most potent activity (DPPH: 892.22 mg Trolox equivalent (TE)\/g; ABTS: 916.21 mg TE\/g; CUPRAC: 1082.69 mg TE\/g; FRAP: 915.05 mg TE\/g). Enzyme inhibition assays highlighted the efficacy of P. istanbulicum extracts against key enzymes, with potential implications for managing Alzheimer\u2019s disease, hyperpigmentation, and type 2 diabetes. Cytotoxicity tests against various cancer cell lines showed notable activity, particularly with the aqueous extract on the HGC-27 cell line (IC50: 29.21 \u00b5g\/mL), indicating potential for targeted anti-cancer therapy. Molecular docking and molecular dynamics simulations further supported the binding affinities of quercetin and myricetin derivatives to cancer-related proteins, suggesting significant therapeutic potential. This study underscores the value of P. istanbulicum as a source of bioactive compounds with applications in antioxidant, anti-cancer, and enzyme-inhibitory treatments.<\/jats:p>","DOI":"10.3390\/plants13233421","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T11:27:53Z","timestamp":1733398073000},"page":"3421","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Using In Vitro and In Silico Analysis to Investigate the Chemical Profile and Biological Properties of Polygonum istanbulicum Extracts"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6651-9794","authenticated-orcid":false,"given":"Giancarlo","family":"Angeles Flores","sequence":"first","affiliation":[{"name":"Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy"},{"name":"Botanic Garden \u201cGiardino dei Semplici\u201d, Department of Pharmacy, \u201cGabriele d\u2019Annunzio\u201d University, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaia","family":"Cusumano","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2635-6553","authenticated-orcid":false,"given":"Mehmet Veysi","family":"Cetiz","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0853-108X","authenticated-orcid":false,"given":"Abdullahi Ibrahim","family":"Uba","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetic, Faculty of Science and Arts, Istanbul Arel University, Istanbul 34537, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2656-0319","authenticated-orcid":false,"given":"Ismail","family":"Senkardes","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, Pharmacy Faculty, Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Koyuncu","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ozgur","family":"Yuksekdag","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Kalyniukova","sequence":"additional","affiliation":[{"name":"Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Kam\u00fdck\u00e1 129, 165 21 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Emiliani","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7768-0468","authenticated-orcid":false,"given":"Roberto","family":"Venanzoni","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6862-1079","authenticated-orcid":false,"given":"Paola","family":"Angelini","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"184","DOI":"10.3390\/ddc3010011","article-title":"Plant-derived natural products: A source for drug discovery and development","volume":"3","author":"Chaachouay","year":"2024","journal-title":"Drugs Drug Candidates"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wang, Z., Guo, S., Cai, Y., Yang, Q., Wang, Y., Yu, X., Sun, W., Qiu, S., Li, X., and Guo, Y. 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