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Two clinically important parasites of high prevalence rates are Trichinella spiralis and Strongyloides stercoralis. However, the limitations of currently used nematocidal drugs highlight the urgent need for novel treatment approaches. The present study investigated the in vitro nematocidal activity of methanol extracts from Amphipterygium adstringens, Artemisia ludoviciana, Cymbopogon citratus, Heterotheca inuloides, Jatropha dioica, Justicia spicigera, Larrea tridentata, Mimosa tenuiflora, Psacalium decompositum, Ruta chalepensis, Semialarium mexicanum, and Smilax aspera against T. spiralis L1 and S. venezuelensis L3 (model for S. stercoralis). Most of the plants showed antiparasitic activity, but R. chalepensis crude methanol extract showed the most potent nematocidal activity against both parasites, with a mean lethal concentration (LC50) of 28.2 \u00b5g\/mL and a selectivity index (SI) of 22.4 for T. spiralis and an LC50 of 244.8 \u00b5g\/mL and SI of 2.58 for S. venezuelensis. This extract was further separated into n-hexane, chloroform, and methanol partitions by continuous Soxhlet extractions. The n-hexane partition demonstrated the strongest activity against both parasites, with an LC50 of 147.6 \u00b5g\/mL and an SI of 7.77 against T. spiralis and an LC50 of 39.2 \u00b5g\/mL and an SI of 3.77 against S. venezuelensis. LC-MS\/MS analysis identified coumarins as the main chemical class (53%), and chalepin represented this partition\u2019s most abundant compound (29.9%). Overall, this study confirmed the antiparasitic potential of medicinal plants commonly used in Mexico. In addition, it highlights the possibility of obtaining bioactive compounds from plants like R. chalepensis, or the other plants evaluated in this study, as novel treatments against parasitic diseases.<\/jats:p>","DOI":"10.3390\/plants13243484","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T10:53:27Z","timestamp":1734000807000},"page":"3484","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3201-5437","authenticated-orcid":false,"given":"Nancy E.","family":"Rodr\u00edguez-Garza","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"},{"name":"Grupo de Enfermedades Infecciosas y Tropicales (e-INTRO), Instituto de Investigaci\u00f3n Biom\u00e9dica de Salamanca\u2014Centro de Investigaci\u00f3n de Enfermedades Tropicales de la Universidad de Salamanca (IBSAL-CIETUS), Facultad de Farmacia, Universidad de Salamanca, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9518-4433","authenticated-orcid":false,"given":"Ricardo","family":"Gomez-Flores","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, 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Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4013-8974","authenticated-orcid":false,"given":"C\u00e9sar I.","family":"Romo-S\u00e1enz","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, Nuevo Le\u00f3n, Mexico"},{"name":"Facultad de Medicina y Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Chihuahua, Chihuahua 31109, Chihuahua, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8768-2507","authenticated-orcid":false,"given":"Miguel","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas y Tropicales (e-INTRO), Instituto de 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Life, 13.","DOI":"10.3390\/life13040969"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Bai, H., Tang, B., Qiao, W., Wu, X., Liu, M., and Wang, X. (2022). The prevalence of Trichinella spiralis in domestic pigs in China: A systematic review and meta-analysis. Animals, 12.","DOI":"10.3390\/ani12243553"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"119","DOI":"10.2478\/helm-2021-0022","article-title":"Foodborne parasitic diseases in the neotropics\u2014A review","volume":"58","author":"Coria","year":"2021","journal-title":"Helminthologia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e2021009","DOI":"10.4178\/epih.e2021009","article-title":"Strongyloides stercoralis and other intestinal parasites in patients receiving immunosuppressive drugs in Northern Iran: A closer look at risk factors","volume":"43","author":"Mirzaei","year":"2021","journal-title":"Epidemiol. Health"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Luvira, V., Siripoon, T., Phiboonbanakit, D., Somsri, K., Watthanakulpanich, D., and Dekumyoy, P. (2022). Strongyloides stercoralis: A neglected but fatal parasite. Trop. Med. Infect. Dis., 7.","DOI":"10.3390\/tropicalmed7100310"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s13071-020-04549-6","article-title":"Screening of Strongyloides stercoralis infection in high-risk patients in Khuzestan Province, Southwestern Iran","volume":"14","author":"Ashiri","year":"2021","journal-title":"Parasit. Vectors"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Koshy, A.A. (2021). Parasitic infections of the central nervous system. Aminoff\u2019s Neurol. Gen. Med., 821\u2013837.","DOI":"10.1016\/B978-0-12-819306-8.00046-0"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1017\/S0031182016000834","article-title":"Human infection with Strongyloides stercoralis and other related strongyloides species","volume":"144","author":"Nutman","year":"2017","journal-title":"Parasitology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1080\/14656566.2022.2114829","article-title":"Current pharmacotherapeutic strategies for strongyloidiasis and the complications in its treatment","volume":"23","author":"Buonfrate","year":"2022","journal-title":"Expert. Opin. Pharmacother."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"El-Wakil, E.S., Shaker, S., Aboushousha, T., Abdel-Hameed, E.-S.S., and Osman, E.E.A. (2023). In vitro and in vivo anthelmintic and chemical studies of Cyperus rotundus L. extracts. BMC Complement. Med. Ther., 23.","DOI":"10.1186\/s12906-023-03839-7"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"ElGhannam, M., Dar, Y., ElMehlawy, M.H., Mokhtar, F.A., and Bakr, L. (2023). Eugenol; Effective anthelmintic compound against foodborne parasite Trichinella spiralis muscle larvae and adult. Pathogens, 12.","DOI":"10.3390\/pathogens12010127"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e130","DOI":"10.1017\/S0022149X20000048","article-title":"Nematocidal effect of Piper Retrofractum Vahl on morphology and ultrastructure of Strongyloides stercoralis third-stage infective larvae","volume":"94","author":"Riyong","year":"2020","journal-title":"J. Helminthol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Patra, J.K., Das, G., Fraceto, L.F., Campos, E.V.R., Rodriguez-Torres, M.d.P., Acosta-Torres, L.S., Diaz-Torres, L.A., Grillo, R., Swamy, M.K., and Sharma, S. (2018). Nano based drug delivery systems: Recent developments and future prospects. J. Nanobiotechnology, 16.","DOI":"10.1186\/s12951-018-0392-8"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Maestrini, M., Tava, A., Mancini, S., Tedesco, D., and Perrucci, S. (2020). In vitro anthelmintic activity of saponins from Medicago spp. against sheep gastrointestinal nematodes. Molecules, 25.","DOI":"10.3390\/molecules25020242"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ikram, N.K.B.K., and Simonsen, H.T. (2017). A Review of Biotechnological Artemisinin Production in Plants. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.01966"},{"key":"ref_19","first-page":"e202418306","article-title":"Biosynthetic Origin of the Methoxy Group in Quinine and Related Alkaloids","volume":"7","author":"Lombe","year":"2024","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Cruz-P\u00e9rez, A.L., Barrera-Ramos, J., Bernal-Ram\u00edrez, L.A., Bravo-Avilez, D., and Beatriz, R.-A. (2021). Actualized inventory of medicinal plants used in traditional medicine in Oaxaca, Mexico. J. Ethnobiol. Ethnomed., 17.","DOI":"10.1186\/s13002-020-00431-y"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Garza, N.E., Quintanilla-Licea, R., Romo-S\u00e1enz, C.I., Elizondo-Luevano, J.H., Tamez-Guerra, P., Rodr\u00edguez-Padilla, C., and Gomez-Flores, R. (2023). In vitro biological activity and lymphoma cell growth inhibition by selected Mexican medicinal plants. Life, 13.","DOI":"10.3390\/life13040958"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Elizondo-Lu\u00e9vano, J.H., Gomez-Flores, R., Verde-Star, M.J., Tamez-Guerra, P., Romo-S\u00e1enz, C.I., Ch\u00e1vez-Montes, A., Rodr\u00edguez-Garza, N.E., and Quintanilla-Licea, R. (2022). In vitro cytotoxic activity of methanol extracts of selected medicinal plants traditionally used in Mexico against human hepatocellular carcinoma. Plants, 11.","DOI":"10.3390\/plants11212862"},{"key":"ref_23","unstructured":"Biblioteca Digital de la Medicina Tradicional Mexicana (UNAM) (2024, November 24). Atlas de las plantas de la Medicina Tradicional Mexicana. Available online: http:\/\/www.medicinatradicionalmexicana.unam.mx\/apmtm\/index.html."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Beknazarova, M., Whiley, H., and Ross, K. (2016). Advocating for both environmental and clinical approaches to control human strongyloidiasis. Pathogens, 5.","DOI":"10.3390\/pathogens5040059"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7098","DOI":"10.1021\/jf2013474","article-title":"Aliphatic ketones from Ruta chalepensis (Rutaceae) induce paralysis on root knot nematodes","volume":"59","author":"Ntalli","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1017\/S0022149X16000419","article-title":"In vitro anthelmintic activity of active compounds of the fringed rue Ruta chalepensis against dairy ewe gastrointestinal nematodes","volume":"91","author":"Ortu","year":"2017","journal-title":"J. Helminthol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"899","DOI":"10.4236\/ajps.2012.37106","article-title":"Obtaining the minimum lethal dose against Fasciola hepatica in vitro using plant extract hexanes with fasciolicide activity and toxicity evaluation on cd1 male mice","volume":"3","year":"2012","journal-title":"Am. J. Plant Sci."},{"key":"ref_28","first-page":"49","article-title":"Phytochemical and in vitro screening of Justicia spicigera ethanol extract for antioxidant activity and in vivo assessment against Schistosoma mansoni infection in mice","volume":"16","author":"Awad","year":"2018","journal-title":"Antiinfect. Agents"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0378-8741(99)00185-3","article-title":"In vitro screening of american plant extracts on Trypanosoma cruzi and Trichomonas vaginalis","volume":"71","author":"Nogal","year":"2000","journal-title":"J. Ethnopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1590\/S1984-29612011000200011","article-title":"Effect of six tropical tanniferous plant extracts on larval exsheathment of Haemonchus contortus","volume":"20","author":"Bevilaqua","year":"2011","journal-title":"Rev. Bras. Parasitol. Veterin\u00e1ria"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3783","DOI":"10.1007\/s00436-021-07281-x","article-title":"Cysticidal effect of a pure naphthoquinone on Taenia crassiceps cysticerci","volume":"120","author":"Castro","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Indrayanto, G., Putra, G.S., and Suhud, F. (2021). Validation of in-vitro bioassay methods: Application in herbal drug research. Profiles of Drug Substances, Excipients and Related Methodology, Elsevier Inc.","DOI":"10.1016\/bs.podrm.2020.07.005"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.vetpar.2017.08.007","article-title":"In vitro anthelmintic and cytotoxicity activities the Digitaria insularis (Poaceae)","volume":"245","author":"Santos","year":"2017","journal-title":"Vet. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cho-Ngwa, F., Abongwa, M., Ngemenya, M.N., and Nyongbela, K.D. (2010). Selective activity of extracts of Margaritaria discoidea and Homalium africanum on Onchocerca ochengi. BMC Complement. Altern. Med., 10.","DOI":"10.1186\/1472-6882-10-62"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Al-Majmaie, S., Nahar, L., Rahman, M.M., Nath, S., Saha, P., Talukdar, A.D., Sharples, G.P., and Sarker, S.D. (2021). Anti-MRSA constituents from Ruta chalepensis (Rutaceae) grown in Iraq, and in silico studies on two of most active compounds, chalepensin and 6-hydroxy-rutin 3\u2032,7-dimethyl ether. Molecules, 26.","DOI":"10.3390\/molecules26041114"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1111\/php.13610","article-title":"Enhanced accumulation of biologically active coumarin and furanocoumarins in callus culture and field-grown plants of Ruta chalepensis through LED light-treatment","volume":"98","author":"Juneja","year":"2022","journal-title":"Photochem. Photobiol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bailly, C. (2023). Ruta angustifolia Pers. (narrow-leaved fringed rue): Pharmacological properties and phytochemical profile. Plants, 12.","DOI":"10.3390\/plants12040827"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2","DOI":"10.2174\/2772432817666220510143902","article-title":"A candidate for health promotion, disease prevention and treatment, common rue (Ruta graveolens L.), an important medicinal plant in traditional medicine","volume":"19","author":"Shahrajabian","year":"2022","journal-title":"Curr. Rev. Clin. Exp. Pharmacol."},{"key":"ref_39","first-page":"1934578X2110658","article-title":"Bioactivity-guided isolation of TRAIL-resistance-overcoming activity compounds from the leaves of Murraya exotica","volume":"16","author":"Jitrangsri","year":"2021","journal-title":"Nat. Prod. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Widyawaruyanti, A., Tanjung, M., Permanasari, A.A., Saputri, R., Tumewu, L., Adianti, M., Aoki-Utsubo, C., Hotta, H., Hafid, A.F., and Wahyuni, T.S. (2021). Alkaloid and benzopyran compounds of Melicope latifolia fruit exhibit anti-hepatitis c virus activities. BMC Complement. Med. Ther., 21.","DOI":"10.1186\/s12906-021-03202-8"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2454","DOI":"10.1021\/np200780m","article-title":"From retrospective assessment to prospective decisions in natural product isolation: HPLC-SPE-NMR analysis of Carthamus oxyacantha","volume":"74","author":"Johansen","year":"2011","journal-title":"J. Nat. Prod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1021\/np300464t","article-title":"Bioactive dimeric carbazole alkaloids from Murraya koenigii","volume":"76","author":"Uvarani","year":"2013","journal-title":"J. Nat. Prod."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wiart, C. (2014). Phenolics. Lead Compounds from Medicinal Plants for the Treatment of Neurodegenerative Diseases, Academic Press.","DOI":"10.1016\/B978-0-12-398373-2.00003-0"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Pal, D., and Saha, S. (2020). Coumarins: An important phytochemical with therapeutic potential. Plant-Deriv. Bioact., 205\u2013222.","DOI":"10.1007\/978-981-15-2361-8_9"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"109505","DOI":"10.1016\/j.vetpar.2021.109505","article-title":"In vitro anthelmintic evaluation of three alkaloids against gastrointestinal nematodes of goats","volume":"296","author":"Branco","year":"2021","journal-title":"Vet. Parasitol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Feng, J., Qin, C., Liu, X., Li, R., Wang, C., Li, C., Du, G., and Guo, Q. (2023). Nematicidal coumarins from Cnidium monnieri fruits and Angelica dahurica roots and their physiological effect on pine wood nematode (Bursaphelenchus xylophilus). Molecules, 28.","DOI":"10.3390\/molecules28104109"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Bazald\u00faa-Rodr\u00edguez, A.F., Quintanilla-Licea, R., Verde-Star, M.J., Hern\u00e1ndez-Garc\u00eda, M.E., Vargas-Villarreal, J., and Garza-Gonz\u00e1lez, J.N. (2021). Furanocoumarins from Ruta chalepensis with amebicide activity. Molecules, 26.","DOI":"10.3390\/molecules26123684"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1351\/pac200173030617","article-title":"Strategies for the isolation and identification of trypanocidal compounds from the rutales","volume":"73","author":"Vieira","year":"2001","journal-title":"Pure Appl. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Nahar, L., Al-Majmaie, S., Al-Groshi, A., Rasul, A., and Sarker, S.D. (2021). Chalepin and chalepensin: Occurrence, biosynthesis and therapeutic potential. Molecules, 26.","DOI":"10.3390\/molecules26061609"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"461770","DOI":"10.1016\/j.chroma.2020.461770","article-title":"Selective extraction of bioactive compounds from plants using recent extraction techniques: A review","volume":"1635","author":"Lefebvre","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.vetpar.2008.10.040","article-title":"Prevalence of Trichinella spp. in North Spain wild fauna and new variety of Trichinella britovi identification","volume":"159","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Mohammed, M.M.D., Heikal, E.A., Ellessy, F.M., Aboushousha, T., and Ghareeb, M.A. (2023). Comprehensive chemical profiling of Bassia indica Wight. aerial parts extract using UPLC-ESI\u2013MS\/MS, and its antiparasitic activity in Trichinella spiralis infected mice: In silico supported in vivo study. BMC Complement. Med. Ther., 23.","DOI":"10.1186\/s12906-023-03988-9"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1186\/s13071-016-1648-5","article-title":"In vitro and in vivo evaluation of 2-aminoalkanol and 1,2-alkanediamine derivatives against Strongyloides venezuelensis","volume":"9","author":"Escarcena","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"108043","DOI":"10.1016\/j.exppara.2020.108043","article-title":"Berberine: A nematocidal alkaloid from Argemone mexicana against Strongyloides venezuelensis","volume":"220","author":"Vicente","year":"2021","journal-title":"Exp. Parasitol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Mar\u00edn, M., S\u00e1nchez-Montejo, J., Ramos, S., Muro, A., L\u00f3pez-Ab\u00e1n, J., and Pel\u00e1ez, R. (2024). Deciphering chemical rules for drug penetration into strongyloides. Pharmaceutics, 16.","DOI":"10.3390\/pharmaceutics16091224"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Elizondo-Lu\u00e9vano, J.H., Rodr\u00edguez-Garza, N.E., Bazald\u00faa-Rodr\u00edguez, A.F., Romo-S\u00e1enz, C.I., Tamez-Guerra, P., Verde-Star, M.J., Gomez-Flores, R., and Quintanilla-Licea, R. (2023). Cytotoxic, anti-hemolytic, and antioxidant activities of Ruta chalepensis L. (Rutaceae) extract, partitions, and isolated compounds. Plants, 12.","DOI":"10.3390\/plants12112203"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ram\u00edrez-Villalobos, J.M., Romo-S\u00e1enz, C.I., Mor\u00e1n-Santiba\u00f1ez, K.S., Tamez-Guerra, P., Quintanilla-Licea, R., Orozco-Flores, A.A., Romero-Arguelles, R., Tamez-Guerra, R., Rodr\u00edguez-Padilla, C., and Gomez-Flores, R. (2021). In vitro tumor cell growth inhibition induced by Lophocereus marginatus (DC.) S. Arias and Terrazas endophytic fungi extracts. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18189917"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Mansour, K.A., El-Neketi, M., Lahloub, M.-F., and Elbermawi, A. (2022). Nanoemulsions of Jasminum humile L. and Jasminum grandiflorum L. essential oils: An approach to enhance their cytotoxic and antiviral effects. Molecules, 27.","DOI":"10.3390\/molecules27113639"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Garza, N.E., Quintanilla-Licea, R., Gomez-Flores, R., Galaviz-Silva, L., and Molina-Garza, Z.J. (2024). In vitro antiprotozoal activity of Schinus molle extract, partitions, and partitions against Trypanosoma cruzi. Plants, 13.","DOI":"10.3390\/plants13162177"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Contreras-Angulo, L.A., Moreno-Ulloa, A., Carballo-Casta\u00f1eda, R.A., Le\u00f3n-Felix, J., Romero-Quintana, J.G., Aguilar-Medina, M., Ramos-Pay\u00e1n, R., and Heredia, J.B. (2022). Metabolomic analysis of phytochemical compounds from agricultural residues of eggplant (Solanum melongena L.). 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