{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T10:55:34Z","timestamp":1787914534411,"version":"build-2784847793"},"reference-count":64,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tunisian Ministry of High Education and Scientific Research, University of Tunis El Manar, Faculty of sciences of Tunis, Laboratory of Molecular Genetics, Immunology, and Biotechnology","award":["LR99ES12"],"award-info":[{"award-number":["LR99ES12"]}]},{"name":"Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura (CEBAS-CSIC) in the Plant Breeding Department","award":["LR99ES12"],"award-info":[{"award-number":["LR99ES12"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Assessing and determining genetic diversity in rose species is a crucial step for conservation efforts, the establishment of a core collection, and the development of new varieties. This study represents the first investigation of genetic diversity among various rose species at different ploidy levels in Tunisia, with the aim of elucidating the genetic structure of the Rosa genus. It encompasses both spontaneous and cultivated accessions, featuring local and introduced species recognized for their adaptability, ornamental value, and fragrance. A total of 114 accessions representing eight rose species were collected. Significant genetic diversity was assessed using seven SSR markers, yielding an average of 21 alleles per locus and a PIC value ranging from 0.882 to 0.941. The results identified 343 phenotypic alleles across the seven primers, with 72 for the primer RhE2b (LG6), 55 for H10D03 (LG7), and 54 for RhB303 (LG2). One key finding was that most perfumed rose accessions (R. damascena Mill. and R. centifolia L.) are distinct from the other rose accessions, indicating a unique genetic pool for these roses. Another important finding was that the Tunisian accessions of \u2018Rose of Ariana\u2019 were closely related to R. centifolia accessions, commonly known as the \u2018Rose of May\u2019 from Grasse, France. To clarify the phylogeny of this species or subspecies, further molecular studies are warranted. Additionally, nomenclature confusion was identified between R. sempervirens L. and R. canina L. in the northwestern region of Tunisia, indicating that all wild accessions correspond to R. sempervirens.<\/jats:p>","DOI":"10.3390\/plants13243563","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T08:16:07Z","timestamp":1734941767000},"page":"3563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genetic Diversity and Relationships Among Tunisian Wild and Cultivated Rosa L. Species"],"prefix":"10.3390","volume":"13","author":[{"given":"Khouloud","family":"Chtourou","sequence":"first","affiliation":[{"name":"LR99ES12, Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire, Immunologie et Biotechnologie, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 de Tunis El Manar, Tunis 2092, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0380-076X","authenticated-orcid":false,"given":"Juan Alfonso","family":"Salazar","sequence":"additional","affiliation":[{"name":"Department of Plant Breeding, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura\u2014Consejo Superior de Investigaciones Cient\u00edficas (CEBAS-CSIC), Campus Universitario Espinardo, E-30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Germ\u00e1n","family":"Ortu\u00f1o-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Plant Breeding, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura\u2014Consejo Superior de Investigaciones Cient\u00edficas (CEBAS-CSIC), Campus Universitario Espinardo, E-30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2798-3003","authenticated-orcid":false,"given":"Najla","family":"Mezghani","sequence":"additional","affiliation":[{"name":"National Gene Bank of Tunisia, Boulevard Leader Yasser Arafat Z. 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Charguia 1, Tunis 1080, Tunisia"},{"name":"LR21AGR05, Research Laboratory, Management of Horticultural Species in Organic and Conventional System, High Agronomic Institute of Chott Mariem, University of Sousse, Sousse 4042, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neila","family":"Trifi-Farah","sequence":"additional","affiliation":[{"name":"LR99ES12, Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire, Immunologie et Biotechnologie, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 de Tunis El Manar, Tunis 2092, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1054-8814","authenticated-orcid":false,"given":"Pedro","family":"Mart\u00ednez-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Plant Breeding, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura\u2014Consejo Superior de Investigaciones Cient\u00edficas (CEBAS-CSIC), Campus Universitario Espinardo, E-30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9180-6322","authenticated-orcid":false,"given":"Lamia","family":"Krichen","sequence":"additional","affiliation":[{"name":"LR99ES12, Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire, Immunologie et Biotechnologie, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 de Tunis El Manar, Tunis 2092, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.17660\/ActaHortic.2001.547.2","article-title":"Rose Breeding Technologies","volume":"547","author":"Gudin","year":"2001","journal-title":"Acta Hortic."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wissemann, V. 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