{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T17:14:28Z","timestamp":1784049268157,"version":"3.55.0"},"reference-count":96,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Pseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial infections of the urinary tract, upper respiratory tract, gastrointestinal tract, central nervous system, etc. It is possible to develop bacteremia and sepsis in immunocompromised patients. A major problem in treatment is the development of antibiotic resistance. Therefore, new preparations of natural origin are sought, such as plant extracts, which are phytocomplexes and to which it is practically impossible to develop resistance. Geum urbanum L. (Rosacea) is a perennial herb known for many biological properties. This study aimed to investigate the redox-modulating capacity and effect of ethyl acetate (EtOAc) extracts from roots (EtOAcR) and aerial parts (EtOAcAP) of the Bulgarian plant on the phenotype inhibition of the P. aeruginosa Las\/RhI quorum sensing (QS) system, which primarily determines drug resistance in pathogenic bacteria, including biofilm formation, motility, and pigment production. We performed QS assays to account for the effects of the two EtOAc extracts. At sub-minimal inhibitory concentrations (sub-MICs) ranging from 1.56 to 6.25 mg\/mL, the biofilm formation was inhibited 85% and 84% by EtOAcR and 62% and 39% by EtOAcAP extracts, respectively. At the same sub-MICs, the pyocyanin synthesis was inhibited by 17\u201327% after treatment with EtOAcAP and 26\u201330% with EtOAcR extracts. The motility was fully inhibited at 3.12 mg\/mL and 6.25 mg\/mL (sub-MICs). We investigated the inhibitory potential of lasI, lasR, rhiI, and rhiR gene expression in biofilm and pyocyanin probes with the PCR method. Interestingly, the genes were inhibited by two extracts at 3.12 mg\/mL and 6.25 mg\/mL. Antiradical studies, assessed by DPPH, CUPRAC, and ABTS radical scavenging methods and superoxide anion inhibition showed that EtOAcAP extract has effective antioxidant capacity. These results could help in the development of new phytocomplexes that could be applied as biocontrol agents to inhibit the phenotype of the P. aeruginosa QS system and other antibiotic-resistant pathogens.<\/jats:p>","DOI":"10.3390\/plants14020213","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T06:13:07Z","timestamp":1736835187000},"page":"213","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from Geum urbanum L. on the Phenotype Inhibition of the Pseudomonas aeruginosa Las\/RhI Quorum Sensing System"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2577-5625","authenticated-orcid":false,"given":"Lyudmila","family":"Dimitrova","sequence":"first","affiliation":[{"name":"The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3218-4487","authenticated-orcid":false,"given":"Milka","family":"Mileva","sequence":"additional","affiliation":[{"name":"The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1323-2348","authenticated-orcid":false,"given":"Almira","family":"Georgieva","sequence":"additional","affiliation":[{"name":"The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"},{"name":"Institute of Neurobiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elina","family":"Tzvetanova","sequence":"additional","affiliation":[{"name":"The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"},{"name":"Institute of Neurobiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9296-9059","authenticated-orcid":false,"given":"Milena","family":"Popova","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2417-6313","authenticated-orcid":false,"given":"Vassya","family":"Bankova","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3221-2545","authenticated-orcid":false,"given":"Hristo","family":"Najdenski","sequence":"additional","affiliation":[{"name":"The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Omran, B.A., Tseng, B.S., and Baek, K.-H. (2024). Nanocomposites against Pseudomonas aeruginosa biofilms: Recent advances, challenges, and future prospects. Microbiol. Res., 282.","DOI":"10.1016\/j.micres.2024.127656"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1007\/s40265-021-01635-6","article-title":"The epidemiology and pathogenesis and treatment of Pseudomonas aeruginosa infections: An update","volume":"81","author":"Reynolds","year":"2021","journal-title":"Drugs"},{"key":"ref_3","unstructured":"WHO (2024). WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance, WHO."},{"key":"ref_4","unstructured":"CDC (2025, January 08). Antibiotic Resistance Threats in the United States, 2019, Available online: https:\/\/ndc.services.cdc.gov\/wp-content\/uploads\/Antibiotic-Resistance-Threats-in-the-United-States-2019.pdf."},{"key":"ref_5","unstructured":"WHO (2025, January 08). Antimicrobial Resistance Surveillance in Europe 2021\u20132023 Data. 2023. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/antimicrobial-resistance-surveillance-europe-2023-2021-data."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1128\/CMR.19.2.403-434.2006","article-title":"Burn wound infections","volume":"19","author":"Church","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/s10529-007-9620-2","article-title":"Towards understanding Pseudomonas aeruginosa burn wound infections by profiling gene expression","volume":"30","author":"Bielecki","year":"2008","journal-title":"Biotechnol. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/S0305-4179(02)00183-3","article-title":"Septicaemia after burn injury: A comparative study","volume":"28","author":"Bang","year":"2002","journal-title":"Burns"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/0002-9610(80)90329-3","article-title":"A simple method of predicting severe sepsis in burn patients","volume":"139","author":"Baker","year":"1980","journal-title":"Am. J. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.ijid.2014.10.015","article-title":"Role of innate immunity in the pathogenesis of otitis media","volume":"29","author":"Mittal","year":"2014","journal-title":"Int. J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"157","DOI":"10.2165\/11539770-000000000-00000","article-title":"Pseudomonas skin infection: Clinical features, epidemiology, and management","volume":"12","author":"Wu","year":"2011","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mishra, A., Aggarwal, A., and Khan, F. (2024). Medical device-associated infections caused by biofilm-forming microbial pathogens and controlling strategies. Antibiotics, 13.","DOI":"10.3390\/antibiotics13070623"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1002\/bit.27760","article-title":"The role of extracellular DNA in the formation, architecture, stability, and treatment of bacterial biofilms","volume":"118","author":"Panlilio","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1099\/mic.0.28601-0","article-title":"Quorum sensing inhibitors: A bargain of effects","volume":"152","author":"Rasmussen","year":"2006","journal-title":"Microbiology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1128\/AEM.01059-09","article-title":"Identification of catechin as one of the flavonoids from Combretum albiflorum bark extract that reduces the production of quorum-sensing-controlled virulence factors in Pseudomonas aeruginosa PAO1","volume":"76","author":"Vandeputte","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5990","DOI":"10.1128\/JB.182.21.5990-5996.2000","article-title":"Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili","volume":"182","author":"Kohler","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1099\/jmm.0.001116","article-title":"The third quorum-sensing system of Pseudomonas aeruginosa: Pseudomonas quinolone signal and the enigmatic PqsE protein","volume":"69","year":"2020","journal-title":"J. Med. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"14613","DOI":"10.1073\/pnas.251465298","article-title":"A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism","volume":"98","author":"Cao","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0378-1097(03)00849-8","article-title":"Dueling quorum sensing systems in Pseudomonas aeruginosa control the production of the Pseudomonas quinolone signal (PQS)","volume":"230","author":"McGrath","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"501","DOI":"10.3855\/jidc.2543","article-title":"Quorum sensing and virulence of Pseudomonas aeruginosa during urinary tract infections","volume":"6","author":"Senturk","year":"2012","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"101927","DOI":"10.1016\/j.pmpp.2022.101927","article-title":"Quorum sensing and quorum quenching: Two sides of the same coin","volume":"123","author":"Kamath","year":"2023","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.biotechadv.2012.10.004","article-title":"Quorum sensing inhibitors: An overview","volume":"31","author":"Kalia","year":"2013","journal-title":"Biotechnol. Adv."},{"key":"ref_23","unstructured":"Assyov, B., Petrova, A., Dimitrov, D., and Vassilev, R. (2012). Conspectus of the Bulgarian Vascular Flora: Distribution Maps and Floristic Elements, Bulgarian Biodiversity Foundation. [4th ed.]."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"705","DOI":"10.2307\/2960540","article-title":"Geum urbanum L.","volume":"85","author":"Taylor","year":"1997","journal-title":"J. Ecol."},{"key":"ref_25","unstructured":"\u0413\u0440o\u0434\u0437\u0456\u043d\u0441\u044c\u043a\u0438\u0439, \u0410.\u041c. (1992). \u041b\u0456\u043a\u0430\u0440\u0441\u044c\u043a\u0456 \u0440o\u0441\u043b\u0438\u043d\u0438: \u0415\u043d\u0446\u0438\u043a\u043bo\u043f\u0435\u0434\u0456\u044f \u0434o\u0432\u0456\u0434\u043d\u0438\u043a. \u0423\u043a\u0440\u0430\u0457\u043d\u0441\u044c\u043a\u0430 \u0415\u043d\u0446\u0438\u043a\u043bo\u043f\u0435\u0434\u0456\u044f, 124\u2013125. (In Ukrainian)."},{"key":"ref_26","unstructured":"\u041b\u0435\u0432\u0447\u0443\u043a, \u0410.\u041f. (1927). \u041a\u0440o\u0432oo\u0441\u0442\u0430\u043d\u0430\u0432\u043b\u0438\u0432\u0430\u044e\u0449\u0438\u0435 \u0438 \u043c\u0430\u0442o\u0447\u043d\u044b\u0435 \u0441\u0440\u0435\u0434\u0441\u0442\u0432\u0430. \u0422\u0440. \u041d\u0430\u0443\u0447. \u0425\u0438\u043c. \u0424\u0430\u0440\u043c. \u0418\u043d-\u0422\u0430, 3\u201379. (In Russian)."},{"key":"ref_27","unstructured":"\u041a\u044co\u0441\u0435\u0432, \u041f.\u0410. (2001). \u041fo\u043b\u043d\u044b\u0439 \u0421\u043f\u0440\u0430\u0432o\u0447\u043d\u0438\u043a \u041b\u0435\u043a\u0430\u0440\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u0445 \u0420\u0430\u0441\u0442\u0435\u043d\u0438\u0439, \u042d\u041a\u0421\u041c\u041e-\u041f\u0440\u0435\u0441\u0441. (In Russian)."},{"key":"ref_28","first-page":"342","article-title":"\u0411o\u0439\u0447\u0438\u043do\u0432. \u0424\u0438\u0442o\u0442\u0435\u0440\u0430\u043f\u0438\u044f","volume":"Volume 2","year":"1970","journal-title":"\u041c\u0435\u0434\u0438\u0446\u0438\u043d\u0430 \u0438 \u0444\u0438\u0437\u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0430"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Bunse, M., Mail\u00e4nder, L.K., Lorenz, P., Stintzing, F.C., and Kammerer, D.R. (2022). Evaluation of Geum urbanum L. Extracts with Respect to Their Antimicrobial Potential. Chem. Biodivers., 19.","DOI":"10.1002\/cbdv.202100850"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/s13065-017-0343-8","article-title":"Antimicrobial and antioxidant potential of different solvent extracts of the medicinal plant Geum urbanum L.","volume":"11","author":"Dimitrova","year":"2017","journal-title":"Chem. Cent. J."},{"key":"ref_31","first-page":"1934578X20915307","article-title":"Investigation of antioxidant and elastase inhibitory activities of Geum urbanum aerial parts, chemical characterization of extracts guided by chemical and biological assays","volume":"15","author":"Schmitt","year":"2020","journal-title":"Nat. Prod. Commun."},{"key":"ref_32","first-page":"37","article-title":"In vitro anti-diabetic and anti-oxidant activities of Geum species from Iran","volume":"9","author":"Farzaneh","year":"2022","journal-title":"Res. J. Pharmacogn."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zaharieva, M.M., Dimitrova, L.L., Philipov, S., Nikolova, I., Vilhelmova, N., Grozdanov, P., Nikolova, N., Popova, M., Bankova, V., and Konstantinov, S.M. (2021). In vitro antineoplastic and antiviral activity and in vivo toxicity of Geum urbanum L. extracts. Molecules, 27.","DOI":"10.3390\/molecules27010245"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Mileva, M., Dimitrova, L., Popova, M., Bankova, V., Krastev, D., Najdenski, H., Zhelev, Z., Aoki, I., and Bakalova-Zheleva, R. (2021). Redox-modulation, suppression of \u201concogenic\u201d superoxide and induction of apoptosis in Burkitt\u2019s lymphoma cells using Geum urbanum L. extracts. Int. J. Bioautom., 25.","DOI":"10.7546\/ijba.2021.25.4.000795"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/j.bbapap.2016.06.009","article-title":"Mechanistic study of the inhibitory activity of Geum urbanum extract against \u03b1-Synuclein fibrillation","volume":"1864","author":"Lobbens","year":"2016","journal-title":"Biochim. Et Biophys. Acta (BBA)-Proteins Proteom."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1139\/bcb-2017-0313","article-title":"Cardiogenic effects of characterized Geum urbanum extracts on adipose-derived human mesenchymal stem cells","volume":"96","author":"Neshati","year":"2018","journal-title":"Biochem. Cell Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jep.2016.04.030","article-title":"Effects of Geum urbanum L. root extracts and its constituents on polymorphonuclear leucocytes functions. Significance in periodontal diseases","volume":"188","author":"Granica","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/1462-2920.14445","article-title":"Where in the world do bacteria experience oxidative stress?","volume":"21","author":"Imlay","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1093\/jac\/dkq308","article-title":"Mode of action of hydrogen peroxide and other oxidizing agents: Differences between liquid and gas forms","volume":"65","author":"Finnegan","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.critrevonc.2008.07.021","article-title":"Infections and cancer: Established associations and new hypotheses","volume":"70","author":"Franceschi","year":"2009","journal-title":"Crit. Rev. Oncol.\/Hematol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/clpt.2009.254","article-title":"Inflammation and cancer: Causes and consequences","volume":"87","author":"Moore","year":"2010","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Juan, C.A., P\u00e9rez de la Lastra, J.M., Plou, F.J., and P\u00e9rez-Lebe\u00f1a, E. (2021). The chemistry of reactive oxygen species (ROS) revisited: Outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094642"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ray, A., Moore, T.F., Pandit, R., Burke, A.D., and Borsch, D.M. (2023). An Overview of selected bacterial infections in cancer, their virulence factors, and some aspects of infection management. Biology, 12.","DOI":"10.3390\/biology12070963"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"431","DOI":"10.4103\/ijmr.IJMR_339_19","article-title":"Effects of infections on the pathogenesis of cancer","volume":"153","author":"Liardo","year":"2021","journal-title":"Indian J. Med. Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Budisan, L., Zanoaga, O., Braicu, C., Pirlog, R., Covaliu, B., Esanu, V., Korban, S.S., and Berindan-Neagoe, I. (2021). Links between infections, lung cancer, and the immune system. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22179394"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s40121-017-0146-1","article-title":"Infections in cancer patients with solid tumors: A review","volume":"6","author":"Rolston","year":"2017","journal-title":"Infect. Dis. Ther."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Wood, S.J., Kuzel, T.M., and Shafikhani, S.H. (2023). Pseudomonas aeruginosa: Infections, animal modeling, and therapeutics. Cells, 12.","DOI":"10.3390\/cells12010199"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Das, T., Kutty, S.K., Kumar, N., and Manefield, M. (2013). Pyocyanin facilitates extracellular DNA binding to Pseudomonas aeruginosa influencing cell surface properties and aggregation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0058299"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1128\/iai.56.9.2515-2517.1988","article-title":"Measurement of Pseudomonas aeruginosa phenazine pigments in sputum and assessment of their contribution to sputum sol toxicity for respiratory epithelium","volume":"56","author":"Wilson","year":"1988","journal-title":"Infect. Immun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.bios.2014.04.028","article-title":"Electrochemical detection of Pseudomonas aeruginosa in human fluid samples via pyocyanin","volume":"60","author":"Webster","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1080\/000164800454062","article-title":"Concentrations of the Pseudomonas aeruginosa toxin pyocyanin in human ear secretions","volume":"120","author":"Reimer","year":"2000","journal-title":"Acta Oto-Laryngol."},{"key":"ref_52","first-page":"341","article-title":"Anti-quorum sensing potential of Geum urbanum L.","volume":"72","author":"Dimitrova","year":"2019","journal-title":"Comptes Rendus Acad. Bulg. Des Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"147","DOI":"10.22192\/ijarbs.2017.04.04.020","article-title":"Isolation and screening of pyocyanin producing Pseudomonas spp. from soil","volume":"4","author":"Gahlout","year":"2017","journal-title":"Int. J. Adv. Res. Biol. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"S110","DOI":"10.4103\/0973-1296.157709","article-title":"Inhibitory potential of some Romanian medicinal plants against enzymes linked to neurodegenerative diseases and their antioxidant activity","volume":"11","author":"Paun","year":"2015","journal-title":"Pharmacogn. Mag."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1016\/j.apjtb.2016.06.008","article-title":"Phenolic compounds affect production of pyocyanin, swarming motility and biofilm formation of Pseudomonas aeruginosa","volume":"6","author":"Ugurlu","year":"2016","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1080\/14786419.2018.1425844","article-title":"Chemical constituents of Geum urbanum L. roots","volume":"32","author":"Dang","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_57","first-page":"98","article-title":"Uber Inhaltsstoffe des Rhizoms von Geum urbanum L.","volume":"32","author":"Gstirner","year":"1964","journal-title":"Sci. Pharm."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5357","DOI":"10.1080\/07391102.2020.1870563","article-title":"Inhibition of biofilm formation, quorum sensing and other virulence factors in Pseudomonas aeruginosa by polyphenols of Gynura procumbens leaves","volume":"40","author":"Nain","year":"2022","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.micpath.2017.07.018","article-title":"Anti-quorum sensing activity of Pistacia atlantica against Pseudomonas aeruginosa PAO1 and identification of its bioactive compounds","volume":"110","author":"Kordbacheh","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Yang, R., Guan, Y., Zhou, J., Sun, B., Wang, Z., Chen, H., He, Z., and Jia, A. (2018). Phytochemicals from Camellia nitidissima Chi flowers reduce the pyocyanin production and motility of Pseudomonas aeruginosa PAO1. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02640"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Sarabhai, S., Sharma, P., and Capalash, N. (2013). Ellagic acid derivatives from Terminalia chebula Retz. downregulate the expression of quorum sensing genes to attenuate Pseudomonas aeruginosa PAO1 virulence. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0053441"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Velasco, W.J., Pab\u00f3n, L.C., G\u00f3mez, V., Hern\u00e1ndez-Rodr\u00edguez, P., and Pati\u00f1o-Ladino, O.J. (2024). Inhibitory Effect of five naturally occurring compounds on the expression of genes associated with the QS system and some virulence factors in P. aeruginosa. Braz. Arch. Biol. Technol., 67.","DOI":"10.1590\/1678-4324-2024230553"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Kim, J.H., Dong, J., Le, B.H., Lonergan, Z.R., Gu, W., Girke, T., Zhang, W., Newman, D.K., and Martins-Green, M. (2024). Pseudomonas aeruginosa activates quorum sensing, antioxidant enzymes and type VI secretion in response to oxidative stress to initiate biofilm formation and wound chronicity. Antioxidants, 13.","DOI":"10.3390\/antiox13060655"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Zhao, X., Yu, Z., and Ding, T. (2020). Quorum-sensing regulation of antimicrobial resistance in bacteria. Microorganisms, 8.","DOI":"10.3390\/microorganisms8030425"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"19145","DOI":"10.1073\/pnas.1902752116","article-title":"Oxidative stress drives the selection of quorum sensing mutants in the Staphylococcus aureus population","volume":"116","author":"George","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3390\/oxygen2020006","article-title":"Free radical properties, source and targets, antioxidant consumption and health","volume":"2","author":"Martemucci","year":"2022","journal-title":"Oxygen"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"100769","DOI":"10.1016\/j.nmni.2020.100769","article-title":"Evaluation of quorum-sensing inhibitory effects of extracts of three traditional medicine plants with known antibacterial properties","volume":"38","author":"Moradi","year":"2020","journal-title":"New Microbes New Infect."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.3109\/10715762.2012.715371","article-title":"In vitro antioxidant capacity and antigenotoxic properties of protoporphyrin and structurally related tetrapyrroles","volume":"46","author":"Huber","year":"2012","journal-title":"Free. Radic. Res."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Halliwell, B., and Gutteridge, J.M. (2015). Free Radicals in Biology and Medicine, Oxford University Press.","DOI":"10.1093\/acprof:oso\/9780198717478.001.0001"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/BF01289410","article-title":"How superoxide radical damages the cell","volume":"217","author":"Benov","year":"2001","journal-title":"Protoplasma"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"4290","DOI":"10.1021\/jf0502698","article-title":"Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements","volume":"53","author":"Prior","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.bbrc.2018.05.045","article-title":"Mini-Review: Oxidative stress, redox stress or redox success?","volume":"502","author":"Gutteridge","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.cell.2013.12.010","article-title":"Regulation of ferroptotic cancer cell death by GPX4","volume":"156","author":"Yang","year":"2014","journal-title":"Cell"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.chembiol.2008.02.010","article-title":"Synthetic lethal screening identifies compounds activating iron-dependent, nonapoptotic cell death in oncogenic-RAS-harboring cancer cells","volume":"15","author":"Yang","year":"2008","journal-title":"Chem. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/nature05859","article-title":"RAS\u2013RAF\u2013MEK-dependent oxidative cell death involving voltage-dependent anion channels","volume":"447","author":"Yagoda","year":"2007","journal-title":"Nature"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.cell.2012.03.042","article-title":"Ferroptosis: An iron-dependent form of nonapoptotic cell death","volume":"149","author":"Dixon","year":"2012","journal-title":"Cell"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Assefa, S.T., Yang, E.-Y., Chae, S.-Y., Song, M., Lee, J., Cho, M.-C., and Jang, S. (2019). Alpha glucosidase inhibitory activities of plants with focus on common vegetables. Plants, 9.","DOI":"10.3390\/plants9010002"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"19","DOI":"10.4103\/0973-7847.79096","article-title":"\u03b1-glucosidase inhibitors from plants: A natural approach to treat diabetes","volume":"5","author":"Kumar","year":"2011","journal-title":"Pharmacogn. Rev."},{"key":"ref_79","unstructured":"(2006). Clinical Laboratory Testing and In Vitro Diagnostic Test Systems\u2014Susceptibility Testing of Infectious Agents and Evaluation of Performance of Antimicrobial Susceptibility Test Devices\u2014Part 1: Reference Method for Testing the In Vitro Activity of Antimicrobial Agents Against Rapidly Growing Aerobic Bacteria Involved in Infectious Diseases (Standard No. ISO 20776-1:2006)."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/jac\/48.suppl_1.5","article-title":"Determination of minimum inhibitory concentrations","volume":"48","author":"Andrews","year":"2001","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0167-7012(00)00122-6","article-title":"A modified microtiter-plate test for quantification of staphylococcal biofilm formation","volume":"40","year":"2000","journal-title":"J. Microbiol. Methods"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Dimitrova, L., Kaleva, M., Zaharieva, M.M., Stoykova, C., Tsvetkova, I., Angelovska, M., Ilieva, Y., Kussovski, V., Naydenska, S., and Najdenski, H. (2021). Prevalence of antibiotic-resistant Escherichia coli isolated from swine faeces and lagoons in Bulgaria. Antibiotics, 10.","DOI":"10.3390\/antibiotics10080940"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Yin, H., Deng, Y., Wang, H., Liu, W., Zhuang, X., and Chu, W. (2015). Tea polyphenols as an antivirulence compound disrupt quorum-sensing regulated pathogenicity of Pseudomonas aeruginosa. Sci. Rep., 5.","DOI":"10.1038\/srep16158"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"609","DOI":"10.2217\/fmb-2018-0293","article-title":"Pseudomonas species isolated from camel meat: Quorum sensing-dependent virulence, biofilm formation and antibiotic resistance","volume":"14","author":"Osman","year":"2019","journal-title":"Future Microbiol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"104989","DOI":"10.1016\/j.micpath.2021.104989","article-title":"Inverse correlation between biofilm production efficiency and antimicrobial resistance in clinical isolates of Pseudomonas aeruginosa","volume":"157","author":"Yamani","year":"2021","journal-title":"Microb. Pathog."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"100646","DOI":"10.1016\/j.mgene.2020.100646","article-title":"Detection of biofilm formation and assessment of biofilm genes expression in different Pseudomonas aeruginosa clinical isolates","volume":"23","author":"Abdelraheem","year":"2020","journal-title":"Meta Gene"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"101","DOI":"10.12816\/0037098","article-title":"Pseudomonas aeruginosa biofilm formation and quorum sensing lasR gene in patients with wound infection","volume":"25","author":"Erfan","year":"2016","journal-title":"Egypt. J. Med. Microbiol. (EJMM)"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1007\/s12668-020-00757-7","article-title":"lasR\/rhlR expression linked to quorum sensing-mediated biofilm formation in Pseudomonas aeruginosa using gold nanoparticles synthesized with ethnobotanical extracts","volume":"10","author":"Velasco","year":"2020","journal-title":"BioNanoScience"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"104105","DOI":"10.1016\/j.micpath.2020.104105","article-title":"pH-dependent inhibition of AHL-mediated quorum sensing by cell-free supernatant of lactic acid bacteria in Pseudomonas aeruginosa PAO1","volume":"142","author":"Rana","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_90","unstructured":"The European Pharmacopoeia Commission, and European Directorate for the Quality of Medicines & HealthCare (2010). European Pharmacopoeia, Council of Europe."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/0003-2697(71)90370-8","article-title":"Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels","volume":"44","author":"Beauchamp","year":"1971","journal-title":"Anal. Biochem."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.foodchem.2010.04.009","article-title":"Antioxidant properties of modified rutin esters by DPPH, reducing power, iron chelation and human low density lipoprotein assays","volume":"123","author":"Lue","year":"2010","journal-title":"Food Chem."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"7970","DOI":"10.1021\/jf048741x","article-title":"Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method","volume":"52","author":"Apak","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1016\/S0968-0896(03)00214-1","article-title":"Synthesis and bioevaluation of glycosyl ureas as \u03b1-glucosidase inhibitors and their effect on mycobacterium","volume":"11","author":"Tewari","year":"2003","journal-title":"Bioorganic Med. Chem."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/2\/213\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:28:27Z","timestamp":1759919307000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/2\/213"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,14]]},"references-count":96,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["plants14020213"],"URL":"https:\/\/doi.org\/10.3390\/plants14020213","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,14]]}}}