{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T12:28:44Z","timestamp":1766492924071,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,26]],"date-time":"2025-01-26T00:00:00Z","timestamp":1737849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Viburnum opulus L. \u2018Roseum\u2019 is a highly valuable ornamental plant for landscaping, but it has a long propagation cycle and low propagation coefficient. In this study, stem segments with axillary buds from Viburnum opulus L. \u2018Roseum\u2019 were used as explants. We systematically analyzed the use of sodium hypochlorite for the sterilization of explants, as well as the effects of different plant growth regulator combinations and concentrations on shoot bud induction, shoot proliferation, the rooting of tissue-cultured shoots, and the transplanting of the tissue-cultured shoots. A complete rapid propagation technology system for Viburnum opulus L. \u2018Roseum\u2019 was established. The results showed that a disinfection method using 75% ethanol for 30 s and soaking in 5% sodium hypochlorite for 5 min was the most suitable for disinfecting the stem segments of Viburnum opulus L. \u2018Roseum\u2019, which showed low contamination and a 73.33% survival rate. The ideal medium for primary bud induction was WPM (Woody Plant Basal Medium) + 2.0 mg\u00b7L\u22121 6-benzylaminopurine (6-BA) + 0.15 mg\u00b7L\u22121 indole-3-butyric acid solution (IBA) + 25 g\u00b7L\u22121 sucrose. The optimal medium for shoot proliferation was WPM + 1.0 mg\u00b7L\u22121 6-BA + 0.15 mg\u00b7L\u22121 IBA + 25 g\u00b7L\u22121 sucrose, achieving an induction rate of 7.17. For the rooting of tissue-cultured shoots, the most suitable formulation was 1\/2 WPM + 0.3 mg\u00b7L\u22121 naphthaleneacetic acid (NAA) + 0.3 mg\u00b7L\u22121 activated charcoal (AC) + 25 g\u00b7L\u22121 sucrose, which induced robust and developed root systems. This study provides a technical basis for the establishment of a fast propagation system for the industrial production of Viburnum opulus L. \u2018Roseum\u2019.<\/jats:p>","DOI":"10.3390\/plants14030374","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T07:46:29Z","timestamp":1737963989000},"page":"374","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Establishment of a Highly Efficient In Vitro Regeneration System for Viburnum opulus L. \u2018Roseum\u2019"],"prefix":"10.3390","volume":"14","author":[{"given":"Yajing","family":"Ning","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6258-261X","authenticated-orcid":false,"given":"Hao","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhua","family":"Li","sequence":"additional","affiliation":[{"name":"Zhejiang Physical and Chemical Technology Co., Ltd., Hangzhou 310000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengpeng","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0606-4841","authenticated-orcid":false,"given":"Shujuan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,26]]},"reference":[{"key":"ref_1","first-page":"1747","article-title":"Population Structure and Dynamic Characteristics of Five Wild Viburnum Plants in Southwest Hubei, China","volume":"43","author":"Zhao","year":"2023","journal-title":"Guihaia"},{"key":"ref_2","first-page":"71","article-title":"A Preliminary Study of Asexual Propagation Ways of Viburnum macrocephalum","volume":"40","author":"Ma","year":"2019","journal-title":"J. Sichuan For. Sci. Technol."},{"key":"ref_3","first-page":"899","article-title":"Effects of Cadmium Stress on Physiological and Biochemical Indices of Viburnum odoratissimum and V. tinus Seedlings","volume":"29","author":"Du","year":"2010","journal-title":"Chin. J. Ecol."},{"key":"ref_4","first-page":"79","article-title":"Heavy Metal Accumulation Capability of Ten Broadleaved Tree Species to As, Hg, Pb, Cd and Cr","volume":"41","author":"Bi","year":"2012","journal-title":"J. West China For. Sci."},{"key":"ref_5","first-page":"233","article-title":"Cutting Propagation Technique of Viburnum macrocephalum Fort","volume":"46","author":"Chen","year":"2017","journal-title":"Bangladesh J. Bot."},{"key":"ref_6","unstructured":"Bi, X.Y. (2018). Study Breeding Technologies of Viburnum sargenti Koehne. [Master\u2019s Thesis, Northeast Forestry University]."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wijerathna-Yapa, A., and Hiti-Bandaralage, J. (2023). Tissue Culture\u2014A Sustainable Approach to Explore Plant Stresses. Life, 13.","DOI":"10.3390\/life13030780"},{"key":"ref_8","first-page":"41","article-title":"In Vitro Propagation of Viburnum opulus \u201cNanum\u201d","volume":"3","author":"VirVirginia","year":"1985","journal-title":"J. Environ. Hort."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.36103\/ijas.v53i6.1654","article-title":"Micorpropagation of Viburnum opulus (Roseum) by Using Single Nodes","volume":"53","author":"Yaseen","year":"2022","journal-title":"Iraqi J. Agric. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1007\/s11676-017-0569-2","article-title":"An Improvised in Vitro Vegetative Propagation Technique for Bambusa tulda: Influence of Season, Sterilization and Hormones","volume":"29","author":"Bhadrawale","year":"2018","journal-title":"J. For. Res."},{"key":"ref_11","first-page":"72","article-title":"Research Progress on Explant Sterilization Technology of Plant Tissue Culture","volume":"13","author":"Wen","year":"2024","journal-title":"Mod. Agric. Sci. Technol."},{"key":"ref_12","first-page":"129","article-title":"Study on Tissue Culture and Rapid Propagation Technology System of Polygonatum sibiricum Red","volume":"43","author":"Tian","year":"2020","journal-title":"J. Nanjing Norm. Univ. Nat. Sci. Ed."},{"key":"ref_13","first-page":"1","article-title":"Studies on the Technology of Primary Culture of Viburnum sargenti In Vitro","volume":"24","author":"Jian","year":"2015","journal-title":"J. Jilin Agric. Sci. Technol. Univ."},{"key":"ref_14","first-page":"59","article-title":"Optimization of Tissue Culture and Rapid Propagation Technology System of Viburnum sargentii","volume":"49","author":"Wang","year":"2021","journal-title":"Jiangsu Agric. Sci."},{"key":"ref_15","first-page":"91","article-title":"Microsomal Propagation of Viburnum sargentii","volume":"42","author":"Wu","year":"2012","journal-title":"J. Shandong For. Sci. Technol."},{"key":"ref_16","first-page":"57","article-title":"Study on Tissue Culture of Viburnum opulus L.","volume":"16","author":"Zhen","year":"2010","journal-title":"Anhui Agric. Sci. Bull."},{"key":"ref_17","first-page":"23","article-title":"Study on Tissue Culture Rapid Propagation and Tissue Micro-Cuttage Technology of Pyrus Betulifolia","volume":"20","author":"Guan","year":"2024","journal-title":"North. Hortic."},{"key":"ref_18","first-page":"50","article-title":"Establishment and Optimization of Tissue Culture and Regeneration System of Ormosia henryi","volume":"52","author":"Wang","year":"2024","journal-title":"Jiangsu Agric. Sci."},{"key":"ref_19","first-page":"33","article-title":"Tissue Culture and Rapid Propagation of Vaccinium ashei \u2018Tifblue\u2019","volume":"09","author":"Li","year":"2022","journal-title":"North. Hortic."},{"key":"ref_20","first-page":"5","article-title":"Studies on Rapid Propagation of Viburunm farreri in Tissue Culture","volume":"05","author":"Du","year":"2011","journal-title":"J. Hebei For. Sci. Technol."},{"key":"ref_21","first-page":"22","article-title":"Establishment of a Tissue Culture and Rapid Propagation System of Lagerstroemia indica \u2018Xiaoming 1\u2019","volume":"32","author":"Cai","year":"2016","journal-title":"Chin. Agric. Sci. Bull."},{"key":"ref_22","unstructured":"Luo, M.W. (2023). Study on Rapid Propagation and Polyploid Induction of Platostoma palustr. [Master\u2019s Thesis, Guangxi Normal University]."},{"key":"ref_23","unstructured":"Bi, X.Y., and Li, S.J. (2018). Establishment of Tissue Culture and Regeneration System of Viburnum sargentii. Hunan Agric. Sci., 4."},{"key":"ref_24","first-page":"1999","article-title":"Optimization and Transplanting of Seeding Rooting Medium of Armeniaca sibirica","volume":"18","author":"Liu","year":"2020","journal-title":"Mol. Plant Breed."},{"key":"ref_25","first-page":"73","article-title":"Tissue Culture of Aristolochia fordiana Hemsl","volume":"41","author":"Pan","year":"2021","journal-title":"Chin. J. Trop. Agric."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/3\/374\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:36:29Z","timestamp":1759919789000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/3\/374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,26]]},"references-count":25,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["plants14030374"],"URL":"https:\/\/doi.org\/10.3390\/plants14030374","relation":{},"ISSN":["2223-7747"],"issn-type":[{"type":"electronic","value":"2223-7747"}],"subject":[],"published":{"date-parts":[[2025,1,26]]}}}