{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:56:58Z","timestamp":1790740618511,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T00:00:00Z","timestamp":1739145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Recovery and Resilience Plan (NRRP)","award":["D53D23011680006"],"award-info":[{"award-number":["D53D23011680006"]}]},{"name":"European Union\u2014NextGenerationEU","award":["D53D23011680006"],"award-info":[{"award-number":["D53D23011680006"]}]},{"name":"University of Parma","award":["D53D23011680006"],"award-info":[{"award-number":["D53D23011680006"]}]},{"name":"MUR\u2014Italian Ministry of Universities and Research","award":["D53D23011680006"],"award-info":[{"award-number":["D53D23011680006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>(1) Background: Traditionally, hop is propagated using rhizome fragments or herbaceous stem cuttings. Micropropagation, therefore, offers a viable alternative for the large-scale production of healthy, genetically uniform plants, regardless of the season and within confined spaces. A temporary immersion system (TIS) facilitates plant propagation by alternating immersions of microcuttings in liquid culture medium with dry periods, preventing gas accumulation through forced ventilation. (2) Methods: In this study, the response of hop plantlets, cv. Columbus, cultured in media with and without sucrose, in solid and liquid culture systems (PlantformTM bioreactor), was evaluated, considering its effect on morpho-physiological parameters, on the total phenolic content, and on antioxidant capacity. Moreover, to make the TIS more efficient, the effect of immersion duration (three and six minutes every twelve hours) was evaluated. (3) Results: The presence of sucrose in the culture medium improved plant proliferation in both culture systems tested: solid and liquid (particularly for explants immersed for three minutes). In the TIS, plantlets with a higher antioxidant capacity were obtained when sucrose-free culture medium was used. (4) Conclusions: This study confirms the efficacy of the TIS as a hop propagation method but also as a valid tool to produce biomass to be used as a source of bioactive compounds.<\/jats:p>","DOI":"10.3390\/plants14040537","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T11:54:06Z","timestamp":1739274846000},"page":"537","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Influence of Sucrose and Immersion Time on Humulus lupulus L., cv Columbus, Plantlet In Vitro Proliferation and Potentially Bioactive Compound Content"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2573-6516","authenticated-orcid":false,"given":"Valeria","family":"Gianguzzi","sequence":"first","affiliation":[{"name":"Department of Agricultural, Food and Forest Sciences (SAAF), University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0439-2916","authenticated-orcid":false,"given":"Leandra","family":"Leto","sequence":"additional","affiliation":[{"name":"Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27\/A, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6766-9962","authenticated-orcid":false,"given":"Anna","family":"Agosti","sequence":"additional","affiliation":[{"name":"Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27\/A, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Di Fazio","sequence":"additional","affiliation":[{"name":"Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27\/A, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1490-0619","authenticated-orcid":false,"given":"Francesco Paolo","family":"Marra","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Forest Sciences (SAAF), University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6485-7589","authenticated-orcid":false,"given":"Martina","family":"Cirlini","sequence":"additional","affiliation":[{"name":"Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27\/A, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-2349","authenticated-orcid":false,"given":"Benedetta","family":"Chiancone","sequence":"additional","affiliation":[{"name":"Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze 27\/A, 43124 Parma, Italy"},{"name":"Institute of Biophysics, National Research Council (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,10]]},"reference":[{"key":"ref_1","first-page":"289","article-title":"Humulus lupulus\u2014A story that begs to be told, a review","volume":"120","author":"Gastl","year":"2014","journal-title":"J. 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