{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T20:28:36Z","timestamp":1773174516241,"version":"3.50.1"},"reference-count":76,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["101131346"],"award-info":[{"award-number":["101131346"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Ceratonia siliqua L. (Fabaceae) is an evergreen sclerophyllous species that successfully overcomes the challenges of the Mediterranean climate. Commonly, biosynthesis of secondary metabolites is a major reaction of the plants thriving in the Mediterranean formations against temperature stress. Due to concerns about the climate crisis, we studied the impact of 6-day low (5 \u00b0C) and high (40 \u00b0C) temperature stress on young carob seedlings. In stressed plants, mainly the heat-treated, the leaves appear xeromorphic. Parameters of the physiology of the plants such as chlorophyll-a and -b, total phenolic content, and oxidative stress were measured and presented via Principal Component Analysis. Chlorophyll-a and -b contents are inferior in cold-stressed leaves while heat-stressed leaves accumulate more phenolics and experience higher oxidative stress as compared to their cold-stressed counterparts. The phytochemical profile of different extracts obtained from stressed carob leaves was identified so as to gain insight into metabolites produced under stress. Moreover, LC-HRMS\/MS metabolomic workflow was utilized for the discovery of biomarkers, over- or under-regulated in stressed conditions. The antimicrobial activity of carob leaf extract fractions was assessed against six human pathogen strains and three phytopathogen bacterial strains. MeOH-H2O and dichloromethane (DCM) extracts presented notable activity against Candida albicans and Saccharomyces cerevisiae, while DCM extracts inhibited the growth of Erwinia amylovora. We may conclude that carob tree exposure to temperature stress does not have a significant influence on secondary metabolic pathways.<\/jats:p>","DOI":"10.3390\/plants14040557","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T04:37:04Z","timestamp":1739335024000},"page":"557","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Impact of Temperature on the Leaves of Ceratonia siliqua L.: Anatomical Aspect, Secondary Metabolite Analysis, and Antimicrobial Activity of the Extracts"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7395-7768","authenticated-orcid":false,"given":"Aikaterina L.","family":"Stefi","sequence":"first","affiliation":[{"name":"Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0981-3633","authenticated-orcid":false,"given":"Evangelos","family":"Kalampokis","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia C.","family":"Ntroumpogianni","sequence":"additional","affiliation":[{"name":"Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iliana","family":"Katsiadrami","sequence":"additional","affiliation":[{"name":"Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5810-8602","authenticated-orcid":false,"given":"Theodora","family":"Nikou","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1868-794X","authenticated-orcid":false,"given":"Efstathios A.","family":"Katsifas","sequence":"additional","affiliation":[{"name":"Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Gkikas","sequence":"additional","affiliation":[{"name":"Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolaos S.","family":"Christodoulakis","sequence":"additional","affiliation":[{"name":"Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5620-0165","authenticated-orcid":false,"given":"Maria","family":"Halabalaki","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, 15784 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref_1","first-page":"245","article-title":"A theory for Mediterranean plant life","volume":"1","author":"Mitrakos","year":"1980","journal-title":"Acta Oecol.-Oecol. Plant"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"di Castri, F., and Mooney, H.A. (1973). Distribution and Peculiarity of Mediterranean Ecosystems. Mediterranean Type Ecosystems. Origin and Structure, Springer.","DOI":"10.1007\/978-3-642-65520-3"},{"key":"ref_3","first-page":"95","article-title":"Winter low temperatures in Mediterranean-type ecosystems","volume":"Volume 2","author":"Quezel","year":"1982","journal-title":"Ecologia Mediterranea. Revue D\u2019\u00e9cologie Terrestre et Limnique"},{"key":"ref_4","unstructured":"Di Castri, F., Goodall, D.W., and Specht, R. (1981). Adaptive strategies in plants dominating Mediterranean-type ecosystems. Mediterranean-Type Shrublands, Elsevier."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Margaris, N.S., and Mooney, H.A. (1981). Leaf structural diversity in Mediterranean evergreen sclerophylls. Components of Productivity of Mediterranean-Climate Regions-Basic and Applied Aspects, Dr W. Junk Publishers.","DOI":"10.1007\/978-94-009-8683-1"},{"key":"ref_6","unstructured":"Martins-Lou\u00e7\u00e3o, M.A., and Brito de Carvalho, J.H. (1989). A Cultura da Alfarrobeira, Direc\u00e7\u00e3o Geral de Planeamento e Agricultura. Available online: https:\/\/www.semanticscholar.org\/paper\/A-cultura-da-alfarrobeira-Lou%C3%A7%C3%A3o-Carvalho\/48856562cbd8f4e387073ccf17e52b2d0ec0ccb8."},{"key":"ref_7","first-page":"1","article-title":"Distribution patterns of four evergreen sclerophyllous species in Greece","volume":"113","author":"Mitrakos","year":"1991","journal-title":"Bot. Jahrb. Syst."},{"key":"ref_8","first-page":"347","article-title":"Die Temperaturresistenz als okophysiologisches Konstitutionsmerkmal. Quercus ilex und andere Eichenarten des Mittelmeergebietes","volume":"4","author":"Larcher","year":"1969","journal-title":"Oecol. Plant"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1080\/11263500012331350455","article-title":"Temperature stress and survival ability of Mediterranean sclerophyllous plants","volume":"134","author":"Larcher","year":"2000","journal-title":"Plant Biosyst."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Kope\u0107, P. (2024). Climate Change\u2014The Rise of Climate-Resilient Crops. Plants, 13.","DOI":"10.3390\/plants13040490"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"292","DOI":"10.2307\/2406805","article-title":"Convergent evolution of the Mediterranean climate evergreen sclerophyll shrubs","volume":"24","author":"Mooney","year":"1970","journal-title":"Evolution"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1086\/282679","article-title":"Photosynthetic systems of Mediterranean-climate shrubs and trees of California and Chile","volume":"104","author":"Mooney","year":"1970","journal-title":"Am. Nat."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Di Castri, F., and Mooney, H.A. (1973). Mediterranean Type Ecosystems: Origin and Structure, Springer.","DOI":"10.1007\/978-3-642-65520-3"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0098-8472(92)90012-Q","article-title":"Structural diversity and adaptations in some Mediterranean evergreen sclerophyllous species","volume":"32","author":"Christodoulakis","year":"1992","journal-title":"Environ. Exp. Bot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1093\/oxfordjournals.aob.a087757","article-title":"An anatomical study of seasonal dimorphism in the leaves of Phlomis fruticosa","volume":"63","author":"Christodoulakis","year":"1989","journal-title":"Ann. Bot."},{"key":"ref_16","first-page":"291","article-title":"Leaf anatomy of three seasonally dimorphic subshrubs","volume":"11","author":"Christodoulakis","year":"1990","journal-title":"Acta Oecol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/BF01700147","article-title":"Air pollution effects on the leaf structure of Laurus nobilis, an injury-resistant species","volume":"44","author":"Christodoulakis","year":"1990","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e28275","DOI":"10.4161\/psb.28275","article-title":"The role of L-DOPA in plants","volume":"9","author":"Soares","year":"2014","journal-title":"Plant Signal. Behav."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/bies.950160209","article-title":"The flavonoid biosynthetic pathway in plants: Function and evolution","volume":"16","author":"Koes","year":"1994","journal-title":"BioEssays"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/BF00345228","article-title":"Seasonal variation in the production of tannins and cyanogenic glucosides in the chaparral shrub, Heteromeles arbutifolia","volume":"15","author":"Dement","year":"1974","journal-title":"Oecologia"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Orshan, G. (1989). General synthesis. Plant Phenomorphological Studies in Mediterranean Type Ecosystems, Kluwer Academic Publishers.","DOI":"10.1007\/978-94-009-3107-7"},{"key":"ref_22","first-page":"167","article-title":"Sclerophylly, chemical defense and the evolution of the Mediterranean maqui species","volume":"9","author":"Dafni","year":"1991","journal-title":"Fl. Veg. Mundi"},{"key":"ref_23","unstructured":"Fahn, A., and Cutler, D.F. (1992). Xerophytes. Encyclopedia of Plant Anatomy, Borntraeger."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0098-8472(94)90032-9","article-title":"Seasonal changes of carbohydrates, lipids and nitrogen content in sun and shade leaves from four Mediterranean evergreen sclerophylls","volume":"34","author":"Rhizopolou","year":"1994","journal-title":"Environ. Exp. Bot."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1093\/jxb\/48.11.1977","article-title":"The effects of seasons, exposure, enhanced UV-B radiation, and water stress on leaf epicuticular and internal UV-B absorbing capacity of Cistus creticus: A Mediterranean field study","volume":"48","author":"Stephanou","year":"1997","journal-title":"J. Exp. Bot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.flora.2006.09.003","article-title":"Secretory structures and cytochemical investigation of the leaf of Phlomis fruticosa, a seasonally dimorphic subshrub. Secreting activity of the leaf-originating calluses","volume":"202","author":"Nikolakaki","year":"2007","journal-title":"Flora"},{"key":"ref_27","unstructured":"Smirnoff, V. (1995). Antioxidant Systems and Plant Response to the Environment. Environment and Plant Metabolism: Flexibility and Acclimation, BIOS Scientific Publishers."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1046\/j.1469-8137.1999.00490.x","article-title":"Diurnal variations of photosynthesis and dew absorption by leaves in two evergreen shrubs growing in Mediterranean field conditions","volume":"144","author":"Nogues","year":"1999","journal-title":"New Phytol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1104\/pp.125.2.1094","article-title":"Subcellular compartmentation of the diterpene carnosic acid and its derivatives in the leaves of rosemary","volume":"125","author":"Alegre","year":"2001","journal-title":"Plant Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Deda, O., Begou, O., Gika, H., Theodoridis, G., and Agapiou, A. (2023). Optimization of Carob Products Preparation for Targeted LC-MS\/MS Metabolomics Analysis. Metabolites, 13.","DOI":"10.3390\/metabo13050645"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1080\/10408398.2016.1231168","article-title":"Absorption, metabolism, anti-cancer effect and molecular targets of epigallocatechin gallate (EGCG): An updated review","volume":"58","author":"Gan","year":"2018","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.crvi.2008.01.002","article-title":"Water-deficit stress-induced anatomical changes in higher plants","volume":"331","author":"Shao","year":"2008","journal-title":"Comptes Rendus Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.jplph.2005.04.022","article-title":"The role of \u03b1-tocopherol in plant stress tolerance","volume":"162","year":"2005","journal-title":"J. Plant Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"100513","DOI":"10.1016\/j.stress.2024.100513","article-title":"Drought and high heat stresses modify Brassica napus L. leaf cuticular wax","volume":"13","author":"Tomasi","year":"2024","journal-title":"Plant Stress"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s12042-015-9152-1","article-title":"Sulfur metabolism and stress defense responses in plants","volume":"8","author":"Capaldi","year":"2015","journal-title":"Trop. Plant Biol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Sharma, P., Lakra, N., Goyal, A., Ahlawat, Y.K., Zaid, A., and Siddique, K.H.M. (2023). Drought and heat stress mediated activation of lipid signaling in plants: A critical review. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1216835"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.phytol.2014.02.007","article-title":"\u03b1-Tocospiro C, a novel cytotoxic \u03b1-tocopheroid from Cirsium setosum","volume":"8","author":"Yuan","year":"2014","journal-title":"Phytochem. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1104\/pp.102.019265","article-title":"Drought-induced changes in the redox state of \u03b1-tocopherol, ascorbate and the diterpene carnosic acid in chloroplasts of Labiatae species differing in carnosic acid contents","volume":"131","author":"Alegre","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/s00425-003-1126-0","article-title":"New insights into the function of tocopherols in plants","volume":"218","author":"Falk","year":"2004","journal-title":"Planta"},{"key":"ref_40","first-page":"145","article-title":"Protective role of \u03b1-tocopherol on two Vicia faba cultivars against seawater-induced lipid peroxidation by enhancing capacity of antioxidative system","volume":"15","author":"Orabi","year":"2016","journal-title":"J. Saudi Soc. Agric. Sci."},{"key":"ref_41","first-page":"156","article-title":"Some studies on the effect of ascorbic acid and \u03b1-tocopherol on the growth and some chemical composition of Hibiscus rosa-sinensis L. Nubaria","volume":"2","author":"Kandil","year":"2009","journal-title":"Ozean J. Appl. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Akram, N.A., Bashir, R., Ashraf, G., Bashir, S., Ashraf, M., Alyemeni, M.N., Bajguz, A., and Ahmad, P. (2023). Exogenous \u03b1-tocopherol regulates the growth and metabolism of eggplant (Solanum melongena L.) under drought stress. Plants, 12.","DOI":"10.3390\/plants12020237"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1104\/pp.104.040808","article-title":"\u03b2-Amylase induction and the protective role of maltose during temperature shock","volume":"135","author":"Kaplan","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1093\/aob\/mcp177","article-title":"Mesophyll distribution of \u2018antioxidant\u2019 flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance","volume":"104","author":"Agati","year":"2009","journal-title":"Ann. Bot."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.jplph.2010.07.016","article-title":"The biosynthesis of flavonoids is enhanced similarly by UV radiation and root zone salinity in L. vulgare leaves","volume":"168","author":"Agati","year":"2011","journal-title":"J. Plant Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Iwaya-Inoue, M., Sakurai, M., and Uemura, M. (2018). Mechanism of stomatal closure in plants exposed to drought and cold stress. Survival Strategies in Extreme Cold and Desiccation. Advances in Experimental Medicine and Biology, Springer.","DOI":"10.1007\/978-981-13-1244-1"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ullah, S., Khan, M.N., Lodhi, S.S., Ahmed, I., Tayyab, M., Mehmood, T., Din, I.U., Khan, M., Sohail, Q., and Akram, M. (2022). Targeted metabolomics reveals fatty acid abundance adjustments as playing a crucial role in drought-stress response and post-drought recovery in wheat. Front. Genet., 10.","DOI":"10.3389\/fgene.2022.972696"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s11099-011-0001-7","article-title":"Influence of enhanced temperature on photosynthesis, photooxidative damage, and antioxidant strategies in Ceratonia siliqua L. seedlings subjected to water deficit and rewatering","volume":"49","author":"Vieira","year":"2011","journal-title":"Photosynthetica"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"153273","DOI":"10.1016\/j.jplph.2020.153273","article-title":"Exogenous melatonin improves tea quality under moderate high temperatures by increasing epigallocatechin-3-gallate and theanine biosynthesis in Camellia sinensis L.","volume":"253","author":"Li","year":"2020","journal-title":"J. Plant Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.plaphy.2020.03.008","article-title":"Epigallocatechin-3-gallate enhances tomato resistance to tobacco mosaic virus by modulating RBOH1-dependent H2O2 signaling","volume":"150","author":"Zhang","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Li, J., Yang, Y., Sun, K., Chen, Y., Chen, X., and Li, X. (2019). Exogenous melatonin enhances cold, salt, and drought stress tolerance by improving antioxidant defense in tea plant (Camellia sinensis (L.) O. Kuntze). Molecules, 24.","DOI":"10.3390\/molecules24091826"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1111\/pce.12061","article-title":"Plant sugars are crucial players in the oxidative challenge during abiotic stress: Extending the traditional concept","volume":"36","author":"Keunen","year":"2013","journal-title":"Plant Cell Environ."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Al Mamari, H.H. (2021). Phenolic compounds: Classification, chemistry, and updated techniques of analysis and synthesis. Phenolic Compounds: Chemistry, Synthesis, Diversity, Non-Conventional Industrial, Pharmaceutical and Therapeutic Applications, IntechOpen.","DOI":"10.5772\/intechopen.98958"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Hasanuzzaman, M., Ahammed, G.J., and Nahar, K. (2022). Plant Phenolic Compounds for Abiotic Stress Tolerance. Managing Plant Production Under Changing Environment, Springer.","DOI":"10.1007\/978-981-16-5059-8"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"570","DOI":"10.3390\/stresses3030040","article-title":"An Overview of Plant Phenolics and Their Involvement in Abiotic Stress Tolerance","volume":"3","author":"Kumar","year":"2023","journal-title":"Stresses"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"104978","DOI":"10.1016\/j.envexpbot.2022.104978","article-title":"Comparative metabolomic analysis reveals the involvement of catechins in adaptation mechanism to cold stress in tea plant (Camellia sinensis var. sinensis)","volume":"201","author":"Wang","year":"2022","journal-title":"Environ. Exp. Bot."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1055\/s-0032-1328300","article-title":"New concepts, experimental approaches, and dereplication strategies for the discovery of novel phytoestrogens from natural sources","volume":"79","author":"Michel","year":"2013","journal-title":"Planta Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1039\/C9NP00011A","article-title":"Synergy and antagonism in natural product extracts: When 1 + 1 does not equal 2","volume":"36","author":"Caesar","year":"2019","journal-title":"Nat. Prod. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Margaris, N.S., and Mooney, H.A. (1980). Low temperature effects on Mediterranean sclerophylls: An unconventional viewpoint. Components of Productivity of Mediterranean-Climate Regions\u2013Basic and Applied Aspects, Proceedings of the International Symposium on Photosynthesis, Primary Production and Biomass Utilization in Mediterranean-Type Ecosystems, Kassandra, Greece, 13\u201315 September 1980, Springer.","DOI":"10.1007\/978-94-009-8683-1"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Fontana, R., Macchi, G., Caproni, A., Sicurella, M., Buratto, M., Salvatori, F., Pappad\u00e0, M., Manfredini, S., Baldisserotto, A., and Marconi, P. (2022). Control of Erwinia amylovora growth by Moringa oleifera leaf extracts: In vitro and in planta effects. Plants, 11.","DOI":"10.3390\/plants11070957"},{"key":"ref_61","unstructured":"APAT (Agency for the Protection of the Environment and for Technical Services) (2003). Seed Propagation of Mediterranean Trees and Shrubs, APAT."},{"key":"ref_62","unstructured":"Mitrakos, K. (October, January 29). The Botany of Ceratonia. Proceedings of the 2nd International Carob Symposium, Generalitat Valenciana, Conselleriad\u2019 Agricultura I Pesca, Valencia, Spain."},{"key":"ref_63","first-page":"658","article-title":"Carob (Ceratonia siliqua L.)","volume":"Volume 10","author":"Bajaj","year":"1990","journal-title":"Legumes and Oilseed Crops I. Biotechnology in Agriculture and Forestry"},{"key":"ref_64","unstructured":"Tsakaldimi, M.N., and Ganatsas, P.P. (2001, January 2\u20136). Treatments improving seeds germination of two Mediterranean sclerophyll species, Ceratonia siliqua and Pistacia lentiscus. Proceedings of the 3rd Balkan Scientific Conference, Sofia, Bulgaria."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0367-2530(17)30630-8","article-title":"Germination ecology of Ceratonia siliqua L. (Caesalpiniaceae), a Mediterranean tree","volume":"190","author":"Ortiz","year":"1995","journal-title":"Flora"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1083\/jcb.17.1.208","article-title":"The use of lead citrate at a high pH as an electron opaque stain in electron microscopy","volume":"17","author":"Reynolds","year":"1963","journal-title":"J. Cell Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1071\/BT08210","article-title":"Structural and cytochemical investigation on the leaf of silverleaf nightshade (Solanum elaeagnifolium), a drought-resistant alien weed of the Greek flora","volume":"57","author":"Christodoulakis","year":"2009","journal-title":"Aust. J. Bot."},{"key":"ref_68","first-page":"199","article-title":"Anatomical and cytochemical investigation on the leaf of Teucrium polium L., a pharmaceutical shrub of the Greek phryganic formations","volume":"14","author":"Christodoulakis","year":"2010","journal-title":"J. Biol. Res."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Gechev, T., Mehterov, N., Denev, I., and Hille, J. (2013). A simple and powerful approach for isolation of Arabidopsis mutants with increased tolerance to H2O2. Induced Cell Death Methods in Enzymology, Elsevier Inc.","DOI":"10.1016\/B978-0-12-405882-8.00011-8"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1042\/bst0110591","article-title":"Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents","volume":"11","author":"Lichtenthaler","year":"1983","journal-title":"Biochem. Soc. Trans."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1093\/jexbot\/52.365.2375","article-title":"Comparison of two methods used to analyse lipid peroxidation from Vaccinium myrtillus (L.) during snow removal, reacclimation and cold acclimation","volume":"52","author":"Taulavuori","year":"2001","journal-title":"J. Exp. Bot."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_73","unstructured":"Waterman, P.G., and Mole, S. (1994). Analysis of Phenolic Plant Metabolites, Blackwell."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Varympopi, A., Dimopoulou, A., Theologidis, I., Karamanidou, T., Kaldeli Kerou, A., Vlachou, A., Karfaridis, D., Papafotis, D., Hatzinikolaou, D.G., and Tsouknidas, A. (2020). Bactericides based on copper nanoparticles restrain growth of important plant pathogens. Pathogens, 9.","DOI":"10.3390\/pathogens9121024"},{"key":"ref_75","unstructured":"Pedregosa, F., Varoquaux, G., Gramfort, A., Michel, V., Thirion, B., Grisel, O., Blondel, M., Prettenhofer, P., Weiss, R., and Dubourg, V. (2012). SciKit-Learn: Machine Learning in Python. arXiv."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Papadopoulou, S., Stefi, A.L., Meletiou-Christou, M.-S., Christodoulakis, N.S., Gkikas, D., and Rhizopoulou, S. (2023). Structural and physiological traits of compound leaves of Ceratonia siliqua trees grown in urban and suburban ambient conditions. 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