{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:48:24Z","timestamp":1790362104180,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["KSN2312023"],"award-info":[{"award-number":["KSN2312023"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Akebia quinata seeds (AQSs) are used as an analgesic, antiphlogistic, and diuretic in traditional herbal medicine. We developed an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC\u2013MS\/MS) simultaneous component analysis method to analyze eight compounds (chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, hederacolchiside F, hederacoside C, dipsacoside B, akebia saponin D, and \u03b1-hederin) as markers for the quality assessment of AQSs. The separation of the eight analytes was performed in an Acquity UPLC BEH C18 reversed-phase analytical column. The method was validated with respect to linearity (coefficient of determination \u2265 0.994), recovery (90.32\u2013108.18%; relative standard deviation (RSD) &lt; 10.0%), and precision (RSD &lt; 10%). The analysis of the AQSs confirmed that the eight components were found in concentrations of 0.42\u20139.07 mg\/g. The cytotoxicity of the AQS extract and the eight compounds against human cancer cell lines, including MDA-MB-231 (breast), A549 (lung), HCT 116 (colon), AsPC-1 (pancreas), and A2780 (ovarian), was also assessed, with cisplatin used as a positive control. In addition, dipsacoside B showed high cytotoxicity in all cell lines. This assay will help to enhance efficacy and clinical research as well as provide a validated quality assessment of AQS extract and related traditional herbal medicines.<\/jats:p>","DOI":"10.3390\/plants14050669","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T10:12:11Z","timestamp":1740132731000},"page":"669","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Simultaneous Component Analysis of Akebia quinata Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography\u2013Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8156-446X","authenticated-orcid":false,"given":"Chang-Seob","family":"Seo","sequence":"first","affiliation":[{"name":"KM Science Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4191-4063","authenticated-orcid":false,"given":"Jaemoo","family":"Chun","sequence":"additional","affiliation":[{"name":"KM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1741-6323","authenticated-orcid":false,"given":"Kwang Hoon","family":"Song","sequence":"additional","affiliation":[{"name":"KM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"114486","DOI":"10.1016\/j.jep.2021.114486","article-title":"Akebia quinata and Akebia trifoliata\u2014A review of phytochemical composition, ethnopharmacological approaches and biological studies","volume":"280","author":"Maciag","year":"2021","journal-title":"J. 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