{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:57:07Z","timestamp":1760057827726,"version":"build-2065373602"},"reference-count":61,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171866","2024010119JC"],"award-info":[{"award-number":["32171866","2024010119JC"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation Project of Jilin Provincial Science and Technology Department","award":["32171866","2024010119JC"],"award-info":[{"award-number":["32171866","2024010119JC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>A Cypripedium plant was discovered in Wangqing County, Changbai Mountain, Jilin Province. This newly discovered plant of Cypripedium not only inhabits the same natural habitat as Cypripedium calceolus L. and Cypripedium \u00d7 ventricosum Sw. but also has a morphology intermediate between that of C. calceolus and C. \u00d7 ventricosum. Its dorsal sepals, petals, and synsepal are similar to those of C. calceolus, while the color of its lip is intermediate between that of C. calceolus and C. \u00d7 ventricosum. For the purpose of distinguishing the newly discovered plant of Cypripedium from other Cypripedium plants, we provisionally named it W1. To further verify the taxonomic status of W1, we introduced three identified forms of C. \u00d7 ventricosum and conducted molecular biology analyses with W1, C. calceolus, and C. \u00d7 ventricosum. The analyses further confirmed the relationship between W1 and C. \u00d7 ventricosum, and the phylogenetic analysis of the nuclear region demonstrated a close relationship between W1 and C. calceolus. Collectively, the morphological and molecular evidence indicates that W1 is a product of the backcross between C. \u00d7 ventricosum and C. calceolus. Although it shows morphological differences from typical C. \u00d7 ventricosum, it can still be considered a form of C. \u00d7 ventricosum. We further investigated the chloroplast genome of this form of C. \u00d7 ventricosum and determined that its total genome length was 196,850 bp. The genome contains 132 genes, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. By analyzing the phylogenetic position and chloroplast genome of the form of C. \u00d7 ventricosum, this study clarified the relationships among Cypripedium taxa with similar morphological characteristics, laying a foundation for research on orchid evolution and species conservation.<\/jats:p>","DOI":"10.3390\/plants14050772","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T10:15:03Z","timestamp":1740996903000},"page":"772","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unveiling a Meaningful Form of Cypripedium \u00d7 ventricosum Sw. (Cypripedioideae, Orchidaceae) from Changbai Mountain, China: Insights from Morphological, Molecular, and Plastome Analyses"],"prefix":"10.3390","volume":"14","author":[{"given":"Ying","family":"Li","sequence":"first","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7723-0189","authenticated-orcid":false,"given":"Xi","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuze","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shizhuo","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiting","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahui","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingtao","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sulei","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0996-0361","authenticated-orcid":false,"given":"Lifei","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzhu","family":"Dai","sequence":"additional","affiliation":[{"name":"Changchun Academy of Forestry, 5840 Jingyue Street, Jingyuetan Tourist Economic Development Zone, Changchun 130117, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"ref_1","first-page":"183","article-title":"A preliminary assessment of the conservation status of Cypripedium species in the wild","volume":"126","author":"Cribb","year":"1998","journal-title":"Bot. 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