{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:29:12Z","timestamp":1770272952637,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T00:00:00Z","timestamp":1741392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Natural extracts derived from plants have gained attention as potential therapeutic agents for obesity management. Some natural extracts were demonstrated to inhibit pancreatic lipase and alpha amylase, potentially influencing nutrient absorption and contributing to weight management. Pinus nigra subsp. laricio (Poir.) Maire, commonly known as the Calabrian pine or larch pine, is a subspecies of the black pine native to the mountains of southern Italy and Corsica. This study investigated the phytochemical content and antioxidant (DPPH and \u03b2-carotene bleaching assays) and enzymatic (lipase and amylase inhibition) activities of ethanolic extracts from apical shoots and branches, fractionated into n-hexane, dichloromethane, and ethyl acetate. All the extracts were also subjected to a preliminary evaluation of their anti-inflammatory potential by measuring the ability to inhibit nitric oxide (NO) production in RAW 264.7 macrophages. The ethyl acetate branch fraction exhibited the strongest antioxidant activity (DPPH IC50 15.67 \u00b1 0.16 \u03bcg\/mL), while the total branch extract best inhibited pancreatic lipase (IC50 0.62 mg\/mL). Amylase inhibition was strongest in the ethyl acetate apical shoot fraction (IC50 22.05 \u00b1 0.29 \u00b5g\/mL). The branches\u2019 hexane and dichloromethane fractions showed the greatest anti-inflammatory potential, inhibiting NO production in RAW 264.7 cells with IC50 values comparable to the positive control.<\/jats:p>","DOI":"10.3390\/plants14060851","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T09:36:24Z","timestamp":1741599384000},"page":"851","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti-Obesity and Weight Management-Related Antioxidant Potential Properties of Calabrian Pine Extracts: Pinus nigra Subsp. laricio (Poir.) Maire"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-4396-9965","authenticated-orcid":false,"given":"Mary","family":"Fucile","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmine","family":"Lupia","sequence":"additional","affiliation":[{"name":"Mediterranean Ethnobotanical Conservatory, 88054 Sersale, Italy"},{"name":"National Ethnobotanical Conservatory, 85040 Castelluccio Superiore, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Armentano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3741-4261","authenticated-orcid":false,"given":"Mariangela","family":"Marrelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludovica","family":"Zicarelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia-Crina","family":"Toma","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Western University \u201cVasile Goldis\u201d of Arad, L. Rebreanu Street, No. 87, 310048 Arad, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4780-452X","authenticated-orcid":false,"given":"Giancarlo","family":"Statti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6699-005X","authenticated-orcid":false,"given":"Filomena","family":"Conforti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,8]]},"reference":[{"key":"ref_1","unstructured":"Pignatti, S. (2017\u20132019). Flora Europea, Edizioni Edagricole-New Business S.r.l."},{"key":"ref_2","first-page":"199","article-title":"Le pinete di pino laricio. Loro conservazione e loro miglioramento","volume":"Volume 14","author":"Meschini","year":"1955","journal-title":"Atti del Congresso Nazionale di Selvicoltura per il Miglioramento e la Conservazione dei Boschi Italiani"},{"key":"ref_3","unstructured":"Lupia, A., Lupia, C., and Lupia, R. (2017). Etnobotanica in Calabria: Viaggio Alla Scoperta di Antichi Saperi Intorno al Mondo Delle Piante, Rubbettino."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3117","DOI":"10.3390\/ijms12053117","article-title":"Inflammation, oxidative stress, and obesity","volume":"12","author":"Bautista","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kim, E.A., Yang, J.-H., Byeon, E.-H., Kim, W., Kang, D., Han, J., Hong, S.-G., Kim, D.-R., Park, S.-J., and Huh, J.-W. (2021). Anti-obesity effect of pine needle extract on high-fat diet-induced obese mice. Plants, 10.","DOI":"10.3390\/plants10050837"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"511","DOI":"10.33224\/rrch\/2019.64.6.07","article-title":"Chemical compositions and biological activities essential oil from the needles of North African Pinus Pinaster Var","volume":"64","author":"Fkiri","year":"2019","journal-title":"Rev. Roum. Chim."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"855","DOI":"10.3109\/13880209.2013.871641","article-title":"Estonian folk traditional experiences on natural anticancer remedies: From past to the future","volume":"52","author":"Sak","year":"2014","journal-title":"Pharm. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1002\/cbdv.201000185","article-title":"Chemical composition and antimicrobial activity of the essential oil of endemic Dalmatian black pine (Pinus nigra ssp","volume":"8","author":"Politeo","year":"2011","journal-title":"dalmatica). Chem. Biodivers."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7773","DOI":"10.3390\/molecules16097773","article-title":"Chemical, antioxidant and antimicrobial investigations of Pinus cembra L. bark and needles","volume":"16","author":"Apetrei","year":"2011","journal-title":"Molecules"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/S0891-5849(99)00090-8","article-title":"Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (pinus maritima) bark, pycnogenol","volume":"27","author":"Packer","year":"1999","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Perri, M.R., Pellegrino, M., Marrelli, M., Aquaro, S., Cavaliere, F., Grande, F., Occhiuzzi, M.A., Lupia, C., Toma, C.-C., and Conforti, F. (2023). Identification of Pinosylvin in Pinus nigra subsp. laricio: A Naturally Occurring Stilbenoid Suppressing LPS-Induced Expression of Pro-Inflammatory Cytokines and Mediators and Inhibiting the JAK\/STAT Signaling Pathway. Pharmaceuticals, 16.","DOI":"10.3390\/ph16050718"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"281","DOI":"10.2174\/1568010054022024","article-title":"Macrophages in Inflammation","volume":"4","author":"Fujiwara","year":"2005","journal-title":"Curr. Drug Target Inflamm. Allergy"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Nisca, A., \u0218tef\u0103nescu, R., Steg\u0103ru\u0219, D.I., Mare, A.D., Farczadi, L., and Tanase, C. (2021). Comparative study regarding the chemical composition and biological activity of pine (Pinus nigra and P. sylvestris) bark extracts. Antioxidants, 10.","DOI":"10.3390\/antiox10020327"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Platzer, M., Kiese, S., Herfellner, T., Schweiggert-Weisz, U., and Eisner, P. (2021). How does the phenol structure influence the results of the Folin-Ciocalteu assay?. Antioxidants, 10.","DOI":"10.3390\/antiox10050811"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Platzer, M., Kiese, S., Herfellner, T., Schweiggert-Weisz, U., Miesbauer, O., and Eisner, P. (2021). Common trends and differences in antioxidant activity analysis of phenolic substances using single electron transfer based assays. Molecules, 26.","DOI":"10.3390\/molecules26051244"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lee, M.-S., Cho, S.-M., Lee, M.-H., Lee, E.-O., Kim, S.-H., and Lee, H.-J. (2016). Ethanol extract of Pinus koraiensis leaves containing lambertianic acid exerts anti-obesity and hypolipidemic effects by activating adenosine monophosphate-activated protein kinase (AMPK). BMC Complement. Altern. Med., 16.","DOI":"10.1186\/s12906-016-1031-2"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1089\/jmf.2012.2665","article-title":"Protective effect of Pinus koraiensis needle water extract against oxidative stress in HepG2 cells and obese mice","volume":"16","author":"Won","year":"2013","journal-title":"J. Med. Food"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.phytochem.2017.06.002","article-title":"Rapid prediction and identification of lipase inhibitors in volatile oil from Pinus massoniana L. needles","volume":"141","author":"Wang","year":"2017","journal-title":"Phytochemistry"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Lim, W.X.J., Gammon, C.S., von Hurst, P., Chepulis, L., and Page, R.A. (2022). The Inhibitory Effects of New Zealand Pine Bark (Enzogenol\u00ae) on \u03b1-Amylase, \u03b1-Glucosidase, and Dipeptidyl Peptidase-4 (DPP-4) Enzymes. Nutrients, 14.","DOI":"10.3390\/nu14081596"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Lee, M.-H., Park, S., Xu, Y., Kim, J.-E., Han, H., Lee, J.-H., Paik, J.K., and Lee, H.-J. (2022). Ethanol Extract of Pinus koraiensis Leaves Mitigates High Fructose-Induced Hepatic Triglyceride Accumulation and Hypertriglyceridemia. Appl. Sci., 12.","DOI":"10.3390\/app12136745"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Gorjanovi\u0107, S., Mici\u0107, D., Pastor, F., Tosti, T., Kalu\u0161evi\u0107, A., Risti\u0107, S., and Zlatanovi\u0107, S. (2020). Evaluation of Apple Pomace Flour Obtained Industrially by Dehydration as a Source of Biomolecules with Antioxidant, Antidiabetic and Antiobesity Effects. Antioxidants, 9.","DOI":"10.3390\/antiox9050413"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Craciunescu, O., Seciu-Grama, A.-M., Mihai, E., Utoiu, E., Negreanu-Pirjol, T., Lupu, C.E., Artem, V., Ranca, A., and Negreanu-Pirjol, B.-S. (2023). The Chemical Profile, Antioxidant, and Anti-Lipid Droplet Activity of Fluid Extracts from Romanian Cultivars of Haskap Berries, Bitter Cherries, and Red Grape Pomace for the Management of Liver Steatosis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242316849"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Al Mamun, A., Rakib, A., Mandal, M., Kumar, S., Singla, B., and Singh, U.P. (2024). Polyphenols: Role in Modulating Immune Function and Obesity. Biomolecules, 14.","DOI":"10.20944\/preprints202401.0918.v1"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic-Phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/cpr.12020","article-title":"Hypericum perforatum L. subsp. perforatum induces inhibition of free radicals and enhanced phototoxicity in human melanoma cells under ultraviolet light","volume":"46","author":"Menichini","year":"2013","journal-title":"Cell Prolif."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1080\/14786419.2010.545356","article-title":"Comparative chemical composition and antioxidant activity of Calamintha nepeta (L.) Savi subsp. glandulosa (Req.) Nyman and Calamintha grandiflora (L.) Moench (Labiatae)","volume":"26","author":"Conforti","year":"2012","journal-title":"Nat. Prod. Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Grande, F., Marrelli, M., Amodeo, V., Occhiuzzi, M.A., Pinzaru, I., Fucile, M., Dehelean, C.A., Alexa, E., Conforti, F., and Statti, G. (2024). Molecular Docking Studies and In Vitro Activity of Paliurus spina-christi Mill Extracts as Pancreatic Lipase Inhibitors. Antioxidants, 13.","DOI":"10.3390\/antiox13020160"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/6\/851\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:49:32Z","timestamp":1760028572000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/6\/851"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,8]]},"references-count":27,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["plants14060851"],"URL":"https:\/\/doi.org\/10.3390\/plants14060851","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,8]]}}}