{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T01:39:05Z","timestamp":1783129145104,"version":"3.54.6"},"reference-count":108,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Contamination with heavy metal ions from mining activities presents a major environmental issue. This study investigates pollution caused by heavy metals from mining, with a particular emphasis on toxic ions and essential ions for living organisms. It starts by analyzing the sources of pollution and its effects on soil, vegetation, water, and wildlife (propolis produced by honey bees living in natural environments). Propolis is an indicator of environmental contamination by metals, a natural and valuable product of natural ecosystems. As part of the investigation, the contamination with metal cations (Pb2+, Cu2+, Cd2+, Zn2+, As3+, Fe2+, and Sr2+) of the soil, cultivated vegetables (carrot, turnip, onion, potato) was monitored in 9 points in the Ro\u0219ia Montan\u0103 area, Romania, as well as the river that runs through the area. The maximum values of the parameters investigated were recorded in soil (108.32 mg\/kg Pb2+, 23.06 mg\/kg Cd2+, 102.17 mg\/kg As3+), river water (11.00 \u00b5g\/L Pb2+, 903.47 \u00b5g\/L Cu2+, 60.13 \u00b5g\/L Cd2+, 1903.08 \u00b5g\/L Zn2+, 148.07 \u00b5g\/L As3+, 44,024.08 \u00b5g\/L Fe2+), vegetables (0.72 mg\/kg Pb2+, 0.17 mg\/kg Cd2+) and it was followed whether the same heavy metals are found in propolis (maximum values 10.14 mg\/kg Pb2+, 6.32 mg\/kg Cu2+, 0.158 mg\/kg Cd2+, 6.0 Zn2+, 1.04 mg\/kg As3+, 12.06 mg\/kg Sr2+). The parameters analyzed for the river waters were pH, sulfates, the oxygen and nutrient regime, and microbial load. Additional investigations were carried out into the quality of these propolis samples: water activity, moisture, hygroscopicity, water solubility, volatile oils, oxidation index, measuring point, density, dry matter, material insoluble in ethanol, extractable with ethanol, ash, and wax. The highest values were 189.4 mg GAE\/g for phenols, 84.31 mg QE\/g for flavonoids, and 0.086 \u00b5g\/mL for IC50 antioxidant activity. This study indicates that bee products, such as propolis, can be an indicator of pollution in mining areas.<\/jats:p>","DOI":"10.3390\/plants14060866","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T12:02:59Z","timestamp":1741608179000},"page":"866","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Environmental Contamination and Mining Impact: Physico-Chemical and Biological Characterization of Propolis as an Indicator of Pollution in the Ro\u0219ia Montan\u0103 Area, Romania"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5059-9336","authenticated-orcid":false,"given":"Mirel","family":"Glevitzky","sequence":"first","affiliation":[{"name":"Faculty of Informatics and Engineering, \u201c1 Decembrie 1918\u201d University of Alba Iulia, 15-17 Unirii Street, 510009 Alba Iulia, Romania"},{"name":"Sanitary Veterinary and Food Safety Directorate of Alba County, 7A Lalelor Street, 510217 Alba Iulia, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxana","family":"Bostan","sequence":"additional","affiliation":[{"name":"Faculty of Informatics and Engineering, \u201c1 Decembrie 1918\u201d University of Alba Iulia, 15-17 Unirii Street, 510009 Alba Iulia, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0811-0821","authenticated-orcid":false,"given":"Mihaela Laura","family":"Vic\u0103","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400012 Cluj-Napoca, Romania"},{"name":"Institute of Legal Medicine, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2097-5230","authenticated-orcid":false,"given":"Gabriela-Alina","family":"Dumitrel","sequence":"additional","affiliation":[{"name":"Faculty of Industrial Chemistry and Environmental Engineering, Politehnica University Timi\u015foara, 2 Victoriei Square, 300006 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihai-Teopent","family":"Corche\u015f","sequence":"additional","affiliation":[{"name":"Faculty of Informatics and Engineering, \u201c1 Decembrie 1918\u201d University of Alba Iulia, 15-17 Unirii Street, 510009 Alba Iulia, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Popa","sequence":"additional","affiliation":[{"name":"Faculty of Informatics and Engineering, \u201c1 Decembrie 1918\u201d University of Alba Iulia, 15-17 Unirii Street, 510009 Alba Iulia, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana","family":"Glevitzky","sequence":"additional","affiliation":[{"name":"Sanitary Veterinary and Food Safety Directorate of Alba County, 7A Lalelor Street, 510217 Alba Iulia, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horea-Vladi","family":"Matei","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400012 Cluj-Napoca, Romania"},{"name":"Institute of Legal Medicine, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"ref_1","first-page":"133","article-title":"Heavy metal toxicity and the environment","volume":"101","author":"Tchounwou","year":"2012","journal-title":"Exp. 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