{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:56:21Z","timestamp":1787961381210,"version":"build-2784847793"},"reference-count":54,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SECIHTI of Mexico for its Ph.D. scholarship","award":["744624"],"award-info":[{"award-number":["744624"]}]},{"name":"SECIHTI of Mexico for its Ph.D. scholarship","award":["MOD.ORD.10\/2023-I1200\/331\/2023"],"award-info":[{"award-number":["MOD.ORD.10\/2023-I1200\/331\/2023"]}]},{"name":"SECIHTI for the postdoctoral fellowship","award":["744624"],"award-info":[{"award-number":["744624"]}]},{"name":"SECIHTI for the postdoctoral fellowship","award":["MOD.ORD.10\/2023-I1200\/331\/2023"],"award-info":[{"award-number":["MOD.ORD.10\/2023-I1200\/331\/2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Epilepsy is a chronic neurological disorder with significant treatment challenges, necessitating the search for alternative therapies. This study evaluates the anticonvulsant activity and toxicological profile of Verbesina persicifolia leaf extracts. Methanolic and sequential fractions (hexane, dichloromethane, ethyl acetate, and methanol) were tested using a pentylenetetrazole (PTZ)-induced seizure model in zebrafish (Danio rerio), measuring seizure latency, severity, and survival rates. Phytochemical screening confirmed the presence of flavonoids, alkaloids, and steroids, suggesting potential neuroactive properties. The hexane extracts significantly increased seizure latency and survival rates, with co-administration of hexane extract (5 \u00b5g\/mL) and diazepam (35.5 \u00b5M) further enhancing these effects. Toxicity assessment in Artemia salina indicated low to moderate toxicity in methanolic extracts, while sequential fractions exhibited higher toxicity, particularly in hexane and ethyl acetate extracts. These findings suggest that V. persicifolia extracts exert anticonvulsant effects, likely through GABAergic modulation, and exhibit a favorable safety profile at therapeutic doses. The results support further investigations to isolate active constituents, confirm their mechanisms of action, and explore their potential as plant-derived anticonvulsant agents.<\/jats:p>","DOI":"10.3390\/plants14071078","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T05:29:19Z","timestamp":1743485359000},"page":"1078","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anticonvulsant Potential and Toxicological Profile of Verbesina persicifolia Leaf Extracts: Evaluation in Zebrafish Seizure and Artemia salina Toxicity Models"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5427-6377","authenticated-orcid":false,"given":"Carlos Alberto","family":"L\u00f3pez-Rosas","sequence":"first","affiliation":[{"name":"Instituto de Qu\u00edmica Aplicada, Universidad Veracruzana, Luis Castelazo Ayala s\/n, Col. Industrial Animas, Xalapa 91190, Mexico"},{"name":"Instituto de Neuroetolog\u00eda, Universidad Veracruzana, Luis Castelazo Ayala s\/n, Col. Industrial Animas, Xalapa 91190, Mexico"},{"name":"Facultad de Qu\u00edmica Farmac\u00e9utica Biol\u00f3gica, Universidad Veracruzana, Circuito Aguirre Beltr\u00e1n s\/n, Col. Zona UV, Xalapa 91090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3892-2353","authenticated-orcid":false,"given":"Santiago","family":"Gonz\u00e1lez-Peria\u00f1ez","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica Aplicada, Universidad Veracruzana, Luis Castelazo Ayala s\/n, Col. 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C., Carretera Antigua a Coatepec 351, Xalapa 91073, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5846-7891","authenticated-orcid":false,"given":"Margarita Virginia","family":"Saavedra V\u00e9lez","sequence":"additional","affiliation":[{"name":"Instituto de Neuroetolog\u00eda, Universidad Veracruzana, Luis Castelazo Ayala s\/n, Col. Industrial Animas, Xalapa 91190, Mexico"},{"name":"Facultad de Qu\u00edmica Farmac\u00e9utica Biol\u00f3gica, Universidad Veracruzana, Circuito Aguirre Beltr\u00e1n s\/n, Col. Zona UV, Xalapa 91090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2287-7041","authenticated-orcid":false,"given":"Fabiola","family":"Hern\u00e1ndez-Rosas","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n, Universidad Anahuac Quer\u00e9taro, El Marqu\u00e9s, Quer\u00e9taro 76246, Mexico"},{"name":"Escuela de Ingenier\u00eda Biom\u00e9dica, Divisi\u00f3n de Ingenier\u00edas, Universidad Anahuac Quer\u00e9taro, El Marqu\u00e9s, Quer\u00e9taro 76246, Mexico"},{"name":"Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Quer\u00e9taro 76010, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"ref_1","unstructured":"WHO (2024, December 20). 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