{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T04:51:06Z","timestamp":1780116666458,"version":"3.54.0"},"reference-count":55,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T00:00:00Z","timestamp":1746230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CNPq and CAPES","award":["Finance code 001"],"award-info":[{"award-number":["Finance code 001"]}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["Finance code 001"],"award-info":[{"award-number":["Finance code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>This study aimed to assess the production of Smilax brasiliensis seedlings in an in vitro environment and their adaptation to natural conditions, as well as the callus induction, the chemical profile of calli extracts, and their antioxidant potential. The seedlings were obtained from S. brasiliensis seeds germinated in Murashige and Skoog (MS) medium. The germination rate was 33%, and about 22% of the seeds produced whole seedlings. Three-month-old seedlings were acclimatized for two months, resulting in an 80% survival rate and improved physiological characteristics. Callus induction was initiated from leaf explants obtained from seedlings and plant growth regulators (PGRs), with and without light exposure. Calli extracts were obtained using methanol; phenolic compound and flavonoid quantification were performed, and the chemical profile was determined by nuclear magnetic resonance (1H NMR). For comparison, methanol extract from S. brasiliensis leaves collected in Brazilian Cerrado were also analyzed. Antioxidant activity was assessed using the 2,2-diphenyl-1-picryl-hydrazyl method and the ferric-reducing antioxidant power assay. All samples exhibited antioxidant activity according to the methods employed. Furthermore, 1H NMR revealed metabolic profile changes in the calli extracts compared to the leaf extract. This study yielded promising results, suggesting that in vitro culture could improve productivity and conserve the species, although changes were observed in the metabolic profile of S. brasiliensis.<\/jats:p>","DOI":"10.3390\/plants14091383","type":"journal-article","created":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T20:09:14Z","timestamp":1746389354000},"page":"1383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Production of Smilax brasiliensis Seedlings, Callus Induction, Chemical Profile, and Assessment of Antioxidant Activity"],"prefix":"10.3390","volume":"14","author":[{"given":"Paula Avelar","family":"Amado","sequence":"first","affiliation":[{"name":"Campus Centro-Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3155-3241","authenticated-orcid":false,"given":"Ana Hort\u00eancia Fons\u00eaca","family":"Castro","sequence":"additional","affiliation":[{"name":"Campus Centro-Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1220-3724","authenticated-orcid":false,"given":"Lucas Santos","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Campus Centro-Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6454-8946","authenticated-orcid":false,"given":"Mariana Guerra de","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Instituto de Ci\u00eancias Exatas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00facia Pinheiro Santos","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Instituto de Ci\u00eancias Exatas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4130-0304","authenticated-orcid":false,"given":"Luciana Alves Rodrigues dos Santos","family":"Lima","sequence":"additional","affiliation":[{"name":"Campus Centro-Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.jep.2013.02.030","article-title":"Amazonian Brazilian medicinal plants described by C.F.P. von Martius in the 19th century","volume":"147","author":"Breitbach","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_2","first-page":"561","article-title":"Medicinal plants resources in the Cerrado of Mato Grosso State, Brazil: A review","volume":"17","author":"Morais","year":"2003","journal-title":"Acta Bot. Bras."},{"key":"ref_3","unstructured":"Andreata, R.H.P. (2025, January 10). Smilacaceae, Lista de Esp\u00e9cies da Flora do Brasil, Available online: http:\/\/floradobrasil2015.jbrj.gov.br\/FB224."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1200507","DOI":"10.3732\/apps.1200507","article-title":"Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species","volume":"1","author":"Martins","year":"2013","journal-title":"Appl. Plant Sci."},{"key":"ref_5","unstructured":"Andreata, R.H.P. (2003). Smilacaceae. Flora fanerog\u00e2mica do estado de S\u00e3o Paulo, Instituto de Bot\u00e2nica."},{"key":"ref_6","unstructured":"Ryan, I. (2019). Chapter 7: Phytochemical aspects and biological properties of Smilax brasiliensis Sprengel. Phytochemicals: Plant Sources and Potential Health Benefits, Nova Science Publishers. [1st ed.]."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1080\/14786419.2017.1327861","article-title":"Chemical composition, antioxidant and cytotoxic activities of extracts from the leaves of Smilax brasiliensis Sprengel (Smilacaceae)","volume":"32","author":"Amado","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1080\/14786419.2019.1624955","article-title":"Phytotoxic and antioxidant effects of dichloromethane fraction of Smilax brasiliensis Sprengel","volume":"35","author":"Barbosa","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1080\/14786419.2018.1543678","article-title":"Phenolic compounds: Antioxidant and larvicidal potential of Smilax brasiliensis Sprengel leaves","volume":"34","author":"Amado","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.sajb.2017.04.003","article-title":"Antioxidant and allelopathic activities of Smilax brasiliensis Sprengel (Smilacaceae)","volume":"111","author":"Fonseca","year":"2017","journal-title":"South Afr. J. Bot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e110310","DOI":"10.1016\/j.ecoenv.2020.110310","article-title":"Assessment of allelopathic, cytotoxic, genotoxic and antigenotoxic potential of Smilax brasiliensis Sprengel leaves","volume":"192","author":"Amado","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"112781","DOI":"10.1016\/j.foodres.2023.112781","article-title":"Chemical profile, antioxidante potential and toxicity of Smilax brasiliensis Sprengel (Smilacaceae) stems","volume":"168","author":"Silva","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1590\/S1413-70542003000200010","article-title":"Studies of Smilax japecanga Grisebach seed dormancy breakage","volume":"27","author":"Santos","year":"2003","journal-title":"Ci\u00eanc. Agrotec."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mangena, P. (2021). Analysis of correlation between seed vigour, germination and multiple shoot induction in soybean (Glycine max L. Merr.). Helyon, 7.","DOI":"10.1016\/j.heliyon.2021.e07913"},{"key":"ref_15","first-page":"1","article-title":"Estudo do estabelecimento in vitro e indu\u00e7\u00e3o de calos em Banana cv. Grande Naine","volume":"33","author":"Kumar","year":"2019","journal-title":"Rev. Atual Ci\u00eancia Apl. Tecnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1007\/s10529-010-0290-0","article-title":"Acclimatization of tissue cultured plantlets: From laboratory to land","volume":"32","author":"Chandra","year":"2010","journal-title":"Biotechnol. Lett."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"George, E.F., Michael, A., and Klerk, H.G. (2008). Plant Propagation by Tissue Culture, 3 ed., Springer.","DOI":"10.1007\/978-1-4020-5005-3"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Feh\u00e9r, A. (2019). Callus, dedifferentiation, totipotency, somatic embryogenesis: What these terms mean in the era of molecular plant biology?. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00536"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1007\/s12045-012-0086-8","article-title":"Plant tissue culture: Historical developments and applied aspects","volume":"17","author":"Dagla","year":"2012","journal-title":"Resonance"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"143","DOI":"10.5935\/1806-6690.20160017","article-title":"Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)","volume":"47","author":"Castro","year":"2016","journal-title":"Rev. Ci\u00eanc. Agron."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1038\/nprot.2009.237","article-title":"NMR-based metabolomic analysis of plants","volume":"5","author":"Kim","year":"2010","journal-title":"Nat. Protoc."},{"key":"ref_22","first-page":"1276","article-title":"NMR-based metabolomic screening for metabolites associated with resistance to Meloidogyne javanica in Annona muricata roots","volume":"30","author":"Machado","year":"2019","journal-title":"J. Braz. Chem. Soc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/978-1-62703-577-4_9","article-title":"NMR-based metabolomics: A probe to utilize biodiversity","volume":"1055","author":"Pimenta","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0079-6565(95)01017-3","article-title":"Metabolite profiling by one-and two-dimensional NMR analysis of complex mixtures","volume":"28","author":"Fan","year":"1996","journal-title":"Prog. Nucl. Magn. Reson. Spectrosc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jchromb.2014.09.040","article-title":"Structural characterization of steroidal saponins from Smilax trinervula using ultra high-performance liquid chromatography coupled with LtQ-orbitrap mass spectrometry","volume":"974","author":"Liang","year":"2015","journal-title":"J. Chromatogr. B Analyt. Technol. Biomed. Life. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"D\u00edaz, J.G., Vega, S., Ganosa, D., P\u00e9rez de Paz, P., and D\u00edaz Diaz, D. (2025). Novel triterpenes and bioactive compounds isolated from Smilax canariensis Brouss. ex Willd. Separations, 12.","DOI":"10.3390\/separations12040074"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1002\/jccs.201500395","article-title":"Bioguided isolation and antiproliferative activity of constituents from Smilax korthalsii A.D.C. leaves","volume":"63","author":"Hamid","year":"2016","journal-title":"J. Chin. Chem. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Cui, Y., Yang, X., Zhang, D., Li, Y., Zhang, L., Song, B., Yue, Z., Song, X., and Tang, H. (2018). Steroidal constituents from roots and rhizomes of Smilacina japonica. Molecules, 23.","DOI":"10.3390\/molecules23040798"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s11627-010-9327-2","article-title":"Use of biotechnologies for the conservation of plant biodiversity","volume":"47","author":"Engelmann","year":"2010","journal-title":"In Vitro Cell. Dev. Biol.-Plant"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.jep.2018.10.006","article-title":"Introduction to the special issue: Saving plants, saving lives: Trade, sustainable harvest and conservation of traditional medicinals in Asia","volume":"229","author":"Cunningham","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"100462","DOI":"10.1016\/j.tfp.2023.100462","article-title":"Conservation and economic benefits of medicinal plants: Insights from forest-fringe communities of Southwestern Ghana","volume":"14","author":"Asigbaase","year":"2023","journal-title":"Trees For. People"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Normah, M., Chin, H., and Reed, B. (2013). Major Research challenges and directions for future research. Conservation of Tropical Plant Species, Springer.","DOI":"10.1007\/978-1-4614-3776-5"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s13020-016-0108-7","article-title":"Conservation and sustainable use of medicinal plants: Problems, progress, and prospects","volume":"11","author":"Chen","year":"2016","journal-title":"Chin. Med."},{"key":"ref_34","first-page":"117","article-title":"Studies on the seeds of Smilax goyazana A.DC. (Smilacaceae)","volume":"49","author":"Palhares","year":"2009","journal-title":"Phyton"},{"key":"ref_35","first-page":"211","article-title":"Germination trials and domestication potential of three native species with edible sprouts: Ruscus aculeatus L., Tamus communis L. and Smilax aspera L.","volume":"598","author":"Lovato","year":"2003","journal-title":"Acta Hortic."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"105","DOI":"10.17660\/ActaHortic.1999.502.14","article-title":"Germination of medicinal plant Smilax campestris Griseb. (Salsaparrilha)","volume":"502","author":"Rosa","year":"1999","journal-title":"Acta Hortic."},{"key":"ref_37","first-page":"495","article-title":"Germination and seedling morphology of four South American Smilax (Smilacaceae)","volume":"60","author":"Martins","year":"2012","journal-title":"Rev. Biol. Trop."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e55682","DOI":"10.4025\/actasciagron.v44i1.55682","article-title":"Mycorrhization of strawberry plantlets potentiates the synthesis of phytochemicals during ex vitro acclimatization","volume":"44","author":"Chiomento","year":"2022","journal-title":"Acta Sci. Agron."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Terletskaya, N.V., Korbozova, N.K., Kudrina, N.O., Kobylina, T.N., Kurmanbayeva, M.S., Meduntseva, N.D., and Tolstikova, T.G. (2021). The influence of abiotic stress factors on the morphophysiological and phytochemical aspects of the acclimation of the plant Rhodiola semenowii Boriss. Plants, 10.","DOI":"10.3390\/plants10061196"},{"key":"ref_40","first-page":"115","article-title":"In vitro studies of strawberry\u2013an important fruit crop: A review","volume":"31","author":"Palei","year":"2015","journal-title":"J. Plant Sci. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4082","DOI":"10.13057\/biodiv\/d230828","article-title":"Acclimation study of Smilax nageliana A.DC., a climber species endemic to East Java, Indonesia","volume":"23","author":"Sofiah","year":"2022","journal-title":"Biodiversitas"},{"key":"ref_42","first-page":"1","article-title":"Effects of medium salt strength and plant growth regulators on shoot multiplication and root induction of Smilax corbularia","volume":"3","author":"Jirakiattikul","year":"2013","journal-title":"Pharmacologyonline"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"97","DOI":"10.26524\/krj74","article-title":"In vitro callus induction of Smilax wightii A. DC an endemic medicinal plant","volume":"2","author":"Kumar","year":"2015","journal-title":"Kongunadu Res. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A revised medium for a rapid growth and bioassays with tobacco tissues cultures","volume":"15","author":"Murashige","year":"1962","journal-title":"Plant Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1007\/s13562-012-0161-y","article-title":"Establishment of callus from Pyrostegia venusta (Ker Gawl.) Miers and effect of abiotic stress on flavonoids and sterols accumulation","volume":"22","author":"Leyva","year":"2013","journal-title":"J. Plant Biochem. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"106646","DOI":"10.1016\/j.ultsonch.2023.106646","article-title":"A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies","volume":"101","author":"Shen","year":"2023","journal-title":"Ultrason. Sonochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fitote.2014.07.020","article-title":"Triterpenoids of sour jujube show pronounced inhibitory effect on human tumor cells and antioxidant activity","volume":"98","author":"Qiao","year":"2014","journal-title":"Fitoterapia"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Bu\u021b, M.-G., Tero-Vescan, A., Pu\u0219ca\u0219, A., J\u00eetc\u0103, G., and Marc, G. (2024). Exploring the inhibitory potential of phytosterols \u03b2-sitosterol, stigmasterol, and campesterol on 5-alpha reductase activity in the human prostate: An in vitro and in silico approach. Plants, 13.","DOI":"10.3390\/plants13223146"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Bakrim, S., Benkhaira, N., Bourais, I., Benali, T., Lee, L.-H., El Omari, N., Sheikh, R.A., Goh, K.W., Ming, L.C., and Bouyahya, A. (2022). Health benefits and pharmacological properties of stigmasterol. Antioxidants, 11.","DOI":"10.3390\/antiox11101912"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Fratianni, F., d\u2019Acierno, A., Ombra, M.N., Amato, G., De Feo, V., Ayala-Zavala, J.F., Coppola, R., and Nazzaro, F. (2021). Fatty acid composition, antioxidant, and in vitro anti-inflammatory activity of five cold-pressed Prunus seed oils, and their anti-biofilm effect against pathogenic bacteria. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.775751"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jssas.2022.08.001","article-title":"Microwave-assisted extraction of dragon fruit seed oil: Fatty acid profile and functional properties","volume":"22","author":"Boyapati","year":"2023","journal-title":"J. Saudi Soc. Agric. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.foodres.2013.09.010","article-title":"A comparative study of the phenolic compounds and the in vitro antioxidant activity of different Brazilian teas using multivariate statistical techniques","volume":"60","author":"Zielinski","year":"2014","journal-title":"Food Res. Int."},{"key":"ref_53","unstructured":"Brasil, Minist\u00e9rio da Sa\u00fade Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria (2010). Farmacopeia Brasileira, ANVISA. [5th ed]."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e111145","DOI":"10.1016\/j.foodres.2022.111145","article-title":"Antioxidant and anti-inflammatory effects of fractions from ripe fruits of Solanum lycocarpum St. Hil. (Solanaceae) and putative identification of bioactive compounds by GC-MS and LC-DAD-MS","volume":"156","author":"Morais","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_55","unstructured":"Urrea-Victoria, V., Pires, J., Torres, P.B., Santos, D.Y.A.C., and Chow, F. (2016). Ensaio Antioxidante Em Microplaca Do Poder De Redu\u00e7\u00e3o Do Ferro (FRAP) Para Extratos De Algas, Instituto de Bioci\u00eancias, Universidade de S\u00e3o Paulo."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/9\/1383\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:26:39Z","timestamp":1760030799000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/9\/1383"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,3]]},"references-count":55,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["plants14091383"],"URL":"https:\/\/doi.org\/10.3390\/plants14091383","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,3]]}}}