{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T02:13:07Z","timestamp":1785031987789,"version":"3.55.0"},"reference-count":69,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Traditionally, medicinal plants have served as the main resource for treating various human health conditions. Prionosciadium dissectum is a plant used in traditional medicine in the southern region of Jalisco, Mexico, to treat inflammatory respiratory problems. However, this species has not undergone pharmacological or biotechnological studies that validate these popular uses. The aim of this study was to induce calluses on P. dissectum leaves and then evaluate the antioxidant, anti-inflammatory, and antiproliferative activity of their extracts. The best callus induction was obtained using Murashige and Skoog (MS) culture medium with 1 mg\/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg\/L kinetin (KIN). Extracts of hexane, dichloromethane, and methanol were obtained from the dry biomass, and the highest yield was obtained with methanol. The total phenolic content and antioxidant activity of the methanolic extracts were quantified. The methanolic extract showed 26.5 \u00b1 0.4 mg equivalents of gallic acid\/g extract, while, for antioxidant activity, it demonstrated IC50 values of 49.4 \u00b1 0.2 and 10.0 \u00b1 0.0 \u03bcg\/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ((2,2\u2032-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) (ABTS), respectively. Regarding anti-inflammatory potential, the extracts did not significantly affect cell viability in RAW 264.7 macrophages. In contrast, it was clear that all extracts significantly decreased nitric oxide (NO) production at concentrations of 5\u201340 \u00b5g\/mL. Additionally, extracts evaluated in human cancer cell lines only had a significant inhibitory effect at 100 \u00b5g\/mL after 48 h, mainly with dichloromethane extract. This first biotechnological study indicates that P. dissectum cell cultures may produce compounds that favor the biological activities evaluated; however, it is necessary to carry out more in-depth evaluations of its extracts. This study is the basis for future research to enable the sustainable use of this valuable resource.<\/jats:p>","DOI":"10.3390\/plants14091394","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T05:54:43Z","timestamp":1746510883000},"page":"1394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antioxidant, Anti-Inflammatory, and Antiproliferative Activity of a Callus Culture of Prionosciadium dissectum (Apiaceae)"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2811-1143","authenticated-orcid":false,"given":"Antonio","family":"Bernab\u00e9-Antonio","sequence":"first","affiliation":[{"name":"Department of Wood, Pulp and Paper, University Center of Exact Sciences and Engineering, University of Guadalajara, Km 15.5 Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6555-6267","authenticated-orcid":false,"given":"Jessica Nayelli","family":"S\u00e1nchez-Carranza","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Autonomous University of the State of Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Antonio","family":"Silva-Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Wood, Pulp and Paper, University Center of Exact Sciences and Engineering, University of Guadalajara, Km 15.5 Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Romero-Estrada","sequence":"additional","affiliation":[{"name":"Technological Institute Jos\u00e9 Mario Molina Pasquel and Henr\u00edquez, Higher Technological Institute of Jalisco, Tala Academic Unit, Tecnol\u00f3gico Avenue S\/N, Tala 45300, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha Guadalupe","family":"P\u00e9rez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Botany and Zoology, University Center of Biological and Agricultural Sciences, University of Guadalajara, Cam. Ram\u00f3n Padilla S\u00e1nchez 2100, Col. Las Agujas, Zapopan 44600, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7127-7264","authenticated-orcid":false,"given":"Francisco","family":"Cruz-Sosa","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Autonomous Metropolitan University-Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1\u00aa. Secci\u00f3n, Alcald\u00eda Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3558-4803","authenticated-orcid":false,"given":"Mariana","family":"S\u00e1nchez-Ramos","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Autonomous Metropolitan University-Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1\u00aa. Secci\u00f3n, Alcald\u00eda Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9921-732X","authenticated-orcid":false,"given":"Aurelio","family":"Nieto-Trujillo","sequence":"additional","affiliation":[{"name":"Biotic Resources Research Center, Autonomous University of the State of Mexico, Carretera Toluca-Ixtlahuaca Km 14.5, Col. San Cayetano, Toluca 50295, State of Mexico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bansal, Y., Mujib, A., Mamgain, J., Kumar, S., Dewir, Y.H., and Magyar-T\u00e1bori, K. (2024). Synthesis and accumulation of phytocompounds in field-tissue-culture grown (stress) root tissues and simultaneous defense response activity in Glycyrrhiza glabra L.. Sustainability, 16.","DOI":"10.3390\/su16041613"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Maqbool, M., Ishtiaq, M., Mazhar, M.W., Casini, R., Mahmoud, E.A., and Elansary, H.O. (2023). Enhancing bioactive metabolite production in Aerva sanguinolenta callus cultures through silver nanoparticle and salicylic acid elicitation. Sustainability, 15.","DOI":"10.3390\/su151310395"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Al-Qudah, T.S., Shibli, R.A., Zatimeh, A., Tahtamouni, R.W., and Al-Zyoud, F. (2023). A Sustainable approach to in vitro propagation and conservation of Salvia dominica L.: A wild medicinal plant from Jordan. Sustainability, 15.","DOI":"10.3390\/su151914218"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.eng.2018.11.006","article-title":"Biotechnology applications of plant callus cultures","volume":"5","author":"Efferth","year":"2019","journal-title":"Engineering"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"848","DOI":"10.2174\/0929867330666230502113150","article-title":"Natural anticancer agents: Their therapeutic potential, challenges and promising outcomes","volume":"31","author":"Tauro","year":"2024","journal-title":"Curr. Med. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zappavigna, S., Cossu, A.M., Grimaldi, A., Bocchetti, M., Ferraro, G.A., Nicoletti, G.F., Filosa, R., and Caraglia, M. (2020). Anti-Inflammatory drugs as anticancer agents. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21072605"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Nunes, C.d.R., Barreto Arantes, M., Menezes de Faria Pereira, S., Leandro da Cruz, L., de Souza Passos, M., Pereira de Moraes, L., Vieira, I.J.C., and Barros de Oliveira, D. (2020). Plants as sources of anti-inflammatory agents. Molecules, 25.","DOI":"10.3390\/molecules25163726"},{"key":"ref_8","first-page":"79","article-title":"Ethnobotanical applications and their correspondence with phylogeny in Apiaceae-Apioideae","volume":"5","author":"Ajani","year":"2018","journal-title":"Res. J. Pharmacogn."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.indcrop.2017.09.027","article-title":"The Apiaceae: Ethnomedicinal family as source for industrial uses","volume":"109","author":"Talou","year":"2017","journal-title":"Ind. Crop. Prod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.sajb.2020.02.004","article-title":"Traditional medicinal knowledge of Apiaceae at Talassemtane National Park (Northern Morocco)","volume":"131","author":"Redouan","year":"2020","journal-title":"S. Afr. J. Bot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1080\/0972060X.2016.1257957","article-title":"Phytochemical and acaricidal study of the Galbanum, Ferula gumosa Boiss. (Apiaceae) essential oil against Tetranychus urticae Koch (Tetranychidae)","volume":"20","author":"Fatemikia","year":"2017","journal-title":"J. Essent. Oil-Bear. Plants"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/BF02976682","article-title":"Anti-tumor activities of decursinol angelate and decursin from Angelica gigas","volume":"26","author":"Lee","year":"2003","journal-title":"Arch. Pharmacal Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.indcrop.2019.04.033","article-title":"Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients","volume":"135","author":"Zengin","year":"2019","journal-title":"Ind. Crop. Prod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4103\/2225-4110.128904","article-title":"Evaluation of anticancer, antioxidant, and possible anti-inflammatory properties of selected medicinal plants used in Indian traditional medication","volume":"4","author":"Shaikh","year":"2014","journal-title":"J. Tradit. Complement. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1080\/10412905.2020.1787884","article-title":"Cytotoxic effect and molecular docking studies of essential oils of Cymbocarpum erythraeum (DC.) Boiss. (Apiaceae) as potential inhibitors of cholinesterase","volume":"32","author":"Karakaya","year":"2020","journal-title":"J. Essent. Oil Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"108","DOI":"10.14499\/indonesianjpharm31iss2pp108","article-title":"In vitro comparative study for anti-proliferative activity of some plant extracts, Fam. Apiaceae, on HeLa cell line","volume":"31","author":"Gomaa","year":"2020","journal-title":"Indonesian J. Pharm."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/j.fct.2011.01.010","article-title":"Anti-inflammatory activity of Angelica dahurica ethanolic extract on RAW264. 7 cells via upregulation of heme oxygenase-1","volume":"49","author":"Lee","year":"2011","journal-title":"Food Chem. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1002\/ptr.5490","article-title":"Anti-inflammatory activity of constituents isolated from aerial part of Angelica acutiloba Kitagawa","volume":"29","author":"Uto","year":"2015","journal-title":"Phytother. Res."},{"key":"ref_19","first-page":"e00507","article-title":"Assessment of total polyphenols, flavonoids and anti-inflammatory potential of three Apiaceae species grown in the Southeast of Morocco","volume":"9","author":"Derouich","year":"2020","journal-title":"Sci. Afr."},{"key":"ref_20","first-page":"113406","article-title":"In vitro and in vivo anti-inflammatory effects of an ethanol extract from the aerial parts of Eryngium carlinae F. Delaroche (Apiaceae)","volume":"266","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1002\/cbdv.201600039","article-title":"Phytochemical profile, chemotaxonomic studies, and in vitro antioxidant activities of two endemisms from Madeira Archipelago: Melanoselinum decipiens and Monizia edulis (Apiaceae)","volume":"13","author":"Castilho","year":"2016","journal-title":"Chem. Biodiversity"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/j.fct.2017.05.011","article-title":"Antiviral effect of compounds derived from Angelica archangelica L. on Herpes simplex virus-1 and Coxsackievirus B3 infections","volume":"109","author":"Rajtar","year":"2017","journal-title":"Food Chem. Toxicol."},{"key":"ref_23","unstructured":"M\u00e9rillon, J.M., and Ramawat, K. (2017). Nutraceutical potential of Apiaceae. Bioactive Molecules in Food, Springer."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hamidian, M., Salehi, A., Naghiha, R., Dehnavi, M.M., Castangia, I., and Mirfathi, M.N. (2023). The comparative perspective of phytochemistry and biological properties of the Apiaceae family plants. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-39254-8"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Guzm\u00e1n, R.C., Nu\u00f1ez-L\u00f3pez, N.M., Canales-Pi\u00f1a, S., and Morales-Arias, J.G. (2020). Prionosciadium tamayoi (Apiaceae), una especie nueva del occidente de M\u00e9xico. Rev. Mex. Biodivers., 91.","DOI":"10.22201\/ib.20078706e.2020.91.3338"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1021\/np010448t","article-title":"Phytotoxic compounds from Prionosciadium watsoni","volume":"65","author":"Abbas","year":"2002","journal-title":"J. Nat. Prod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1021\/np800387w","article-title":"Dihydrofurochromones from Prionosciadium thapsoides","volume":"71","year":"2008","journal-title":"J. Nat. Prod."},{"key":"ref_28","first-page":"1645","article-title":"Production of anthocyanins in callus cultures of Angelica archangelica","volume":"13","author":"Siatka","year":"2018","journal-title":"Nat. Prod. Commun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"574","DOI":"10.4103\/0973-1296.160443","article-title":"Optimization of induction, subculture conditions, and growth kinetics of Angelica sinensis (Oliv.) Diels callus","volume":"11","author":"Huang","year":"2015","journal-title":"Pharmacogn. Mag."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1007\/s11033-018-4340-3","article-title":"Effect of explant type and plant growth regulators on callus induction, growth and secondary metabolites production in Cnidium officinale Makino","volume":"45","author":"Adil","year":"2018","journal-title":"Mol. Biol. Rep."},{"key":"ref_31","first-page":"53","article-title":"In vitro seed germination and callus induction of Ferula ferulaeoides (Steud.) Korov. (Apiaceae)","volume":"14","author":"Suran","year":"2016","journal-title":"Mong. J. Biol. Sci."},{"key":"ref_32","first-page":"121","article-title":"Establecimiento y multiplicaci\u00f3n in vitro de cinco cultivares de apio (Arracacia xanthorrhiza Bancroft) colectados en Venezuela","volume":"27","author":"Matos","year":"2015","journal-title":"Bioagro"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Golkar, P., Akbari, R., Bazarganipour, M., and Javed, R. (2023). Biochemical and phytochemical responses of Ammi visnaga L. (Apiaceae) callus culture elicited by SiO2 and graphene Oxide-SiO2 nanoparticles. Plant Physiol. Biochem., 200.","DOI":"10.1016\/j.plaphy.2023.107741"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s11738-022-03496-2","article-title":"Elicitors mediated bio-preservative potential targeting seedless fruits using Capsicum annuum L. (Solanaceae) and Foeniculum vulgare Mill. (Apiaceae) calli extracts","volume":"45","author":"Batool","year":"2023","journal-title":"Acta Physiol. Plant."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1080\/14786419.2016.1272105","article-title":"Effects of growth regulators on callus induction and secondary metabolite production in Cuminum cyminum","volume":"31","author":"Farvardin","year":"2017","journal-title":"Nat. Prod. Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Paque, S., and Weijers, D. (2016). Q&A: Auxin: The plant molecule that influences almost anything. BMC Biol., 14.","DOI":"10.1186\/s12915-016-0291-0"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1007\/s12010-015-1618-y","article-title":"Callus growth kinetics of physic nut (Jatropha curcas L.) and content of fatty acids from crude oil obtained in vitro","volume":"176","author":"Bier","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1080\/14786419.2017.1405402","article-title":"Growth kinetics and diosgenin estimation from callus cultures of Costus speciosus (Koen. ex. Retz.)","volume":"32","author":"Hundare","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/j.indcrop.2015.10.004","article-title":"Fatty acid profile of intact plants of two different sites and callus cultures derived from seed and leaf explants of Calophyllum brasiliense Cambess: A new resource of non-edible oil","volume":"77","author":"Toral","year":"2015","journal-title":"Ind. Crop. Prod."},{"key":"ref_40","unstructured":"\u00c1lvarez de Le\u00f3n, R.A., Garc\u00eda Higueros, A.L., Olivia Rentana, N.V., and Rojas Sazo, A.M. (2010). Determinaci\u00f3n de Actividad Inhibitoria In Vitro de Extractos Dicloromet\u00e1nicos y Metan\u00f3licos y Aceites Esenciales de Doce Especies Condimentar\u00edas y Medicinales Guatemaltecas Contra Listeria Monocytogenes. [Bachelor\u2019s Thesis, Universidad de San Carlos de Guatemala, Facultad de Ciencias Qu\u00edmicas y Farmac\u00e9uticas]."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"152","DOI":"10.4067\/S0717-75182009000200007","article-title":"Efecto antioxidante de frutas y hortalizas de la zona central de chile","volume":"36","author":"Palomo","year":"2009","journal-title":"Rev. Chil. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Wu, X.W., Wei, W., Yang, X.W., Zhang, Y.B., Xu, W., Yang, Y.F., Zhong, G.Y., Liu, H.N., and Yang, S.L. (2017). Anti-Inflammatory phenolic acid esters from the roots and rhizomes of Notopterygium incisium and their permeability in the human caco-2 monolayer cell model. Molecules, 22.","DOI":"10.3390\/molecules22060935"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6276438","DOI":"10.1155\/2018\/6276438","article-title":"Alleviation of drugs and chemicals toxicity: Biomedical value of antioxidants","volume":"2018","author":"Aleya","year":"2018","journal-title":"Oxid. Med. Cell Long."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2098123","DOI":"10.1155\/2018\/2098123","article-title":"Aging, metabolic, and degenerative disorders: Biomedical value of antioxidants","volume":"2018","author":"Zakhary","year":"2018","journal-title":"Oxid. Med. Cell Long."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1556\/018.66.2015.4.4","article-title":"In vitro biological activity of secondary metabolites from Seseli rigidum Waldst. et Kit. Apiaceae","volume":"66","year":"2015","journal-title":"Acta Biol. Hung."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lefahal, M., Zaabat, N., Ayad, R., Makhloufi, E.H., Djarri, L., Benahmed, M., Laouer, H., Nieto, G., and Akkal, S. (2018). In vitro assessment of total phenolic and flavonoid contents, antioxidant and photoprotective activities of crude methanolic extract of aerial parts of Capnophyllum peregrinum (L.) Lange (Apiaceae) growing in Algeria. Medicines, 5.","DOI":"10.3390\/medicines5020026"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/5808624","article-title":"Comparative phytochemical constituents and antioxidant activity of wild and cultivated Alepidea amatymbica Eckl & Zeyh","volume":"2020","author":"Mangoale","year":"2020","journal-title":"BioMed Res. Int."},{"key":"ref_48","unstructured":"Puente Vigo, S.L. (2019). Compuestos Bioactivos y Capacidad Antioxidante de Extractos de Hoja de Sacha Culantro (Erynguim foetidum L.) y de Aceite de Copaiba (Copaifera paupera) Procedentes de la Provincia de Coronel Portillo, Ucayali. [Bachelor\u2019s Thesis, Universidad Cient\u00edfica del Sur]."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3971041","DOI":"10.1155\/2020\/3971041","article-title":"Bioactive compounds and antioxidant, antiperoxidative, and antihemolytic properties investigation of three Apiaceae species grown in the southeast of Morocco","volume":"2020","author":"Derouich","year":"2020","journal-title":"Scientifica"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Mottaghipisheh, J., Boveiri Dehsheikh, A., Mahmoodi Sourestani, M., Kiss, T., Hohmann, J., and Csupor, D. (2020). Ducrosia spp., rare plants with promising phytochemical and pharmacological characteristics: An updated review. Pharmaceuticals, 13.","DOI":"10.3390\/ph13080175"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.2042-7158.2010.01170.x","article-title":"Genus Bupleurum: A review of its phytochemistry, pharmacology and modes of action","volume":"63","author":"Ashour","year":"2011","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Kang, H., Bang, T.S., Lee, J.W., Lew, J.H., Eom, S.H., Lee, K., and Choi, H.Y. (2012). Protective effect of the methanol extract from Cryptotaenia japonica Hassk. against lipopolysaccharide-induced inflammation in vitro and in vivo. BMC Complement. Altern. Med., 12.","DOI":"10.1186\/1472-6882-12-199"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.1080\/13880209.2017.1362011","article-title":"Anti-inflammatory effect of torilidis fructus ethanol extract through inhibition of Src","volume":"55","author":"Park","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"653","DOI":"10.7314\/APJCP.2012.13.2.653","article-title":"Eryngium foetidum suppresses inflammatory mediators produced by macrophages","volume":"13","author":"Mekhora","year":"2012","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1271\/bbb.110950","article-title":"Anethum graveloens flower extracts inhibited a lipopolysaccharide-induced inflammatory response by blocking iNOS expression and NF-\u03baB activity in macrophages","volume":"76","author":"Kim","year":"2012","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"125","DOI":"10.2334\/josnusd.58.125","article-title":"Anti-inflammatory and cytotoxic effects of methanol, ethanol, and water extracts of Angelicae dahuricae Radix","volume":"58","author":"Wang","year":"2016","journal-title":"J. Oral Sci."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Kim, H.N., Kim, J.D., Yeo, J.H., Son, H.J., Park, S.B., Park, G.H., Eo, H.J., and Jeong, J.B. (2019). Heracleum moellendorffii roots inhibit the production of pro-inflammatory mediators through the inhibition of NF-\u03baB and MAPK signaling, and activation of ROS\/Nrf2\/HO-1 signaling in LPS-stimulated RAW264.7 cells. BMC Complement. Altern. Med., 19.","DOI":"10.1186\/s12906-019-2735-x"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.1016\/S0024-3205(03)00648-9","article-title":"Acetone extract of Bupleurum scorzonerifolium inhibits proliferation of A549 human lung cancer cells via inducing apoptosis and suppressing telomerase activity","volume":"73","author":"Cheng","year":"2003","journal-title":"Life Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1016\/j.lfs.2004.09.044","article-title":"Requirement for ERK activation in acetone extract identified from Bupleurum scorzonerifolium induced A549 tumor cell apoptosis and keratin 8 phosphorylation","volume":"76","author":"Chen","year":"2005","journal-title":"Life Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/j.lfs.2004.03.008","article-title":"The proliferative inhibition and apoptotic mechanism of saikosaponin D in human non-small cell lung cancer A549 cells","volume":"75","author":"Hsu","year":"2004","journal-title":"Life Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.7314\/APJCP.2014.15.6.2803","article-title":"Antitumor constituents from Anthriscus sylvestris (L.) Hoffm","volume":"15","author":"Chen","year":"2014","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Soyingbe, O.S., Mongalo, N.I., and Makhafola, T.J. (2018). In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm\u2014Medicinal plants used in South Africa. BMC Complement. Altern. Med., 18.","DOI":"10.1186\/s12906-018-2378-3"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"468","DOI":"10.3177\/jnsv.66.468","article-title":"Effects of Coriandrum sativum on migration and invasion abilities of cancer cells","volume":"66","author":"Huang","year":"2020","journal-title":"J. Nut. Sci. Vitaminol."},{"key":"ref_64","unstructured":"INEGI (2025, April 24). Instituto Nacional de Estad\u00edstica y Geograf\u00eda (INEGI). Sistema de Consulta de Mapas, Available online: https:\/\/www.inegi.org.mx\/app\/mapas\/."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A revised medium for rapid growth and bioassays with tobacco tissue cultures","volume":"15","author":"Murashige","year":"1962","journal-title":"Physiol. Plant."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.foodchem.2007.04.038","article-title":"Antioxidant activity and phenolic compounds in 32 selected herbs","volume":"105","author":"Czemerys","year":"2007","journal-title":"Food Chem."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.foodchem.2011.03.090","article-title":"Antioxidant activity of Gardenia jasminoides Ellis fruit extracts","volume":"128","author":"Debnath","year":"2011","journal-title":"Food Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3390\/s30800276","article-title":"Measurement of nitric oxide production in biological systems by using Griess reaction Assay","volume":"3","author":"Sun","year":"2003","journal-title":"Sensors"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1007\/s10495-006-8312-z","article-title":"Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes","volume":"11","author":"Basu","year":"2006","journal-title":"Apoptosis"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/9\/1394\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:27:28Z","timestamp":1760030848000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/9\/1394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,6]]},"references-count":69,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["plants14091394"],"URL":"https:\/\/doi.org\/10.3390\/plants14091394","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,6]]}}}