{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:30:31Z","timestamp":1791808231346,"version":"4.3.4"},"reference-count":62,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Research, Development and Youth of the Slovak Republic Grants","award":["VEGA 1\/0101\/23"],"award-info":[{"award-number":["VEGA 1\/0101\/23"]}]},{"name":"Ministry of Education, Research, Development and Youth of the Slovak Republic Grants","award":["VEGA 1\/0514\/22"],"award-info":[{"award-number":["VEGA 1\/0514\/22"]}]},{"name":"Library Foundation of FaF CU","award":["VEGA 1\/0101\/23"],"award-info":[{"award-number":["VEGA 1\/0101\/23"]}]},{"name":"Library Foundation of FaF CU","award":["VEGA 1\/0514\/22"],"award-info":[{"award-number":["VEGA 1\/0514\/22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Wounding triggers complex secondary metabolic pathways in plants, including benzylisoquinoline alkaloid (BIA) biosynthesis in opium poppy (Papaver somniferum L.). This study explores transcriptional and metabolic responses to wounding and methyl jasmonate (MeJA) treatment, focusing on BIA biosynthesis and regulatory mechanisms. Real-time expression analysis revealed significant up-regulation of transcripts in the (S)-reticuline and papaverine biosynthetic pathway, while the noscapine pathway was suppressed. The morphinan pathway also showed transcriptional activation, except in the case of codeinone reductase (COR), which remained unresponsive to both wounding and MeJA, suggesting a partially uncoupled mechanism. Metabolite profiling using HPLC-MS demonstrated a rapid accumulation of morphine post wounding, further supporting the hypothesis of independent regulatory control over COR. The role of phospholipase C (PLC) in modulating wound-induced BIA accumulation was investigated, revealing that PLC inhibition reduced morphine production and suppressed COR expression. These findings highlight the importance of phospholipid-dependent signalling in activating morphine biosynthesis, potentially at the expense of other BIAs. This study provides insights into plant stress responses and suggests strategies for enhancing BIA production through targeted interventions, offering potential applications in improving alkaloid yield.<\/jats:p>","DOI":"10.3390\/plants14101413","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T11:37:02Z","timestamp":1746704222000},"page":"1413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Wounding and Phospholipase C Inhibition: Evaluation of the Alkaloid Profiling in Opium Poppy"],"prefix":"10.3390","volume":"14","author":[{"given":"Barbora","family":"Hans","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University Bratislava, Kalin\u010diakova 8, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7197-8825","authenticated-orcid":false,"given":"Ema","family":"Bala\u017eov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University Bratislava, Kalin\u010diakova 8, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Dokupilov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"},{"name":"Toxicologic and Antidoping Centre, Faculty of Pharmacy, Comenius University Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5755-9970","authenticated-orcid":false,"given":"Peter","family":"Miku\u0161","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"},{"name":"Toxicologic and Antidoping Centre, Faculty of Pharmacy, Comenius University Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3074-2721","authenticated-orcid":false,"given":"Andrea","family":"Bala\u017eov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University Bratislava, Kalin\u010diakova 8, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8782-5260","authenticated-orcid":false,"given":"Ren\u00e1ta","family":"Kub\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University Bratislava, Kalin\u010diakova 8, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5922-4939","authenticated-orcid":false,"given":"Marek","family":"Oblo\u017einsk\u00fd","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University Bratislava, Kalin\u010diakova 8, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tombuloglu, H., Unver, T., Tombuloglu, G., and Hakeem, K.R. 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