{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:10:43Z","timestamp":1791655843371,"version":"4.3.4"},"reference-count":36,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Government of India"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Lentil (Lens culinaris Medikus) is a critical food crop offering high protein and essential micronutrients. However, its productivity and nutritional quality are increasingly threatened by climate change. In this study, 36 lentil genotypes were evaluated across two Moroccan locations under normal, heat stress, and combined heat and drought stresses. Significant effects of genotype, environment, and their interactions were observed on seed yield, seed size, cooking time, and nutritional quality. Heat and drought stresses caused substantial reductions in seed yield (up to 40% under combined stress), protein content, iron, and zinc concentration, and increased phytic acid levels, which negatively impacted iron and zinc bioavailability. Cooking time significantly decreased under stress conditions, with up to 54% reduction under combined heat and drought stresses at Annoceur research station. Correlation analysis revealed complex trade-offs among yield, nutritional quality, and cooking traits under stress conditions. Principal component analysis and GGE biplot analyses identified genotypes with superior yield, micronutrient concentration, and cooking time stability across environments. Genotypes such as G32, G3, and G36 combined high iron and zinc levels; G13 and G30 showed low phytic acid, while G 15 exhibited the shortest cooking time. These genotypes also demonstrated adaptability across the tested environment. This study highlights the potential of selecting climate-resilient, nutrient-dense lentil genotypes to support breeding efforts aimed at improving food security in the face of global climate variability. These genotypes can be suggested as elite climate-resilient parental lines to support breeders in enhancing lentil yield, nutritional quality, and stability under multiple stress conditions.<\/jats:p>","DOI":"10.3390\/plants14132019","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T10:45:08Z","timestamp":1751366708000},"page":"2019","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Variation of Seed Yield and Nutritional Quality Traits of Lentil (Lens culinaris Medikus) Under Heat and Combined Heat and Drought Stresses"],"prefix":"10.3390","volume":"14","author":[{"given":"Hasnae","family":"Choukri","sequence":"first","affiliation":[{"name":"Laboratoire de Biotechnologie et de Physiologie V\u00e9g\u00e9tales, Facult\u00e9 des Sciences, Centre de Recherche BioBio, University Mohammed V, Rabat 10014, Morocco"},{"name":"International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat 10112, Morocco"},{"name":"AgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic, University, Ben Guerir 43150, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khawla","family":"Aloui","sequence":"additional","affiliation":[{"name":"International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat 10112, Morocco"},{"name":"Laboratory of Ecology and Environment, Ben M\u2019Siki Faculty of Sciences, University Hassan II, Casablanca 20800, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8848-4799","authenticated-orcid":false,"given":"Noureddine","family":"El Haddad","sequence":"additional","affiliation":[{"name":"AgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic, University, Ben Guerir 43150, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3266-1084","authenticated-orcid":false,"given":"Kamal","family":"Hejjaoui","sequence":"additional","affiliation":[{"name":"AgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic, University, Ben Guerir 43150, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelaziz","family":"Smouni","sequence":"additional","affiliation":[{"name":"Laboratoire de Biotechnologie et de Physiologie V\u00e9g\u00e9tales, Facult\u00e9 des Sciences, Centre de Recherche BioBio, University Mohammed V, Rabat 10014, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8407-3562","authenticated-orcid":false,"given":"Shiv","family":"Kumar","sequence":"additional","affiliation":[{"name":"International Center for Agricultural Research in the Dry Areas (ICARDA), Amlaha 466113, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"ref_1","first-page":"423","article-title":"Moisture-Absorption Characteristics of Laird Lentils and Hardshell Seeds","volume":"71","author":"Tang","year":"1994","journal-title":"Cereal Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1094\/CCHEM-05-16-0127-FI","article-title":"Evaluation of Cooking Time in Pulses: A Review","volume":"94","author":"Wood","year":"2017","journal-title":"Cereal Chem."},{"key":"ref_3","unstructured":"(2025, January 04). FAOSTAT Food and Agriculture Organization Corporate Statistical Database. Available online: https:\/\/www.fao.org\/faostat."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Habib-ur-Rahman, M., Ahmad, A., Raza, A., Hasnain, M.U., Alharby, H.F., Alzahrani, Y.M., Bamagoos, A.A., Hakeem, K.R., Ahmad, S., and Nasim, W. (2022). Impact of Climate Change on Agricultural Production; Issues, Challenges, and Opportunities in Asia. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.925548"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12571-011-0124-5","article-title":"Crops That Feed the World 3. Investing in Lentil Improvement toward a Food Secure World","volume":"3","author":"Erskine","year":"2011","journal-title":"Food Secur."},{"key":"ref_6","first-page":"1","article-title":"Global Lentil Production: Constraints and Strategies","volume":"17","author":"Kumar","year":"2013","journal-title":"SATSA Mukhapatra-Annu. Tech."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Jitendra Kumar, J.K., Choudhary, A.K., Gupta, D.S., and Shiv Kumar, S.K. (2019). Towards Exploitation of Adaptive Traits for Climate-Resilient Smart Pulses. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20122971"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Choukri, H., El Haddad, N., Aloui, K., Hejjaoui, K., El-Baouchi, A., Smouni, A., Thavarajah, D., Maalouf, F., and Kumar, S. (2022). Effect of High Temperature Stress during the Reproductive Stage on Grain Yield and Nutritional Quality of Lentil (Lens Culinaris Medikus). Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.857469"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"El Haddad, N., Choukri, H., Ghanem, M.E., Smouni, A., Mentag, R., Rajendran, K., Hejjaoui, K., Maalouf, F., and Kumar, S. (2021). High-Temperature and Drought Stress Effects on Growth, Yield and Nutritional Quality with Transpiration Response to Vapor Pressure Deficit in Lentil. Plants, 11.","DOI":"10.3390\/plants11010095"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Choukri, H., Hejjaoui, K., El-Baouchi, A., El Haddad, N., Smouni, A., Maalouf, F., Thavarajah, D., and Kumar, S. (2020). Heat and Drought Stress Impact on Phenology, Grain Yield, and Nutritional Quality of Lentil (Lens Culinaris Medikus). Front. Nutr., 7.","DOI":"10.3389\/fnut.2020.596307"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5134","DOI":"10.1002\/jsfa.9054","article-title":"Impact of Heat Stress during Seed Filling on Seed Quality and Seed Yield in Lentil (Lens Culinaris Medikus) Genotypes","volume":"98","author":"Sita","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12041-019-1112-3","article-title":"Genetic Dissection of Grain Iron and Zinc Concentrations in Lentil (Lens Culinaris Medik.)","volume":"98","author":"Kumar","year":"2019","journal-title":"J. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2503","DOI":"10.2135\/cropsci2018.05.0321","article-title":"Genotypic Variability and Genotype\u00d7 Environment Interaction for Iron and Zinc Content in Lentil under Nepalese Environments","volume":"58","author":"Shrestha","year":"2018","journal-title":"Crop Sci."},{"key":"ref_14","first-page":"1","article-title":"Genetic Variability and Genotype X Environment Interactions Effect on Grain Iron (Fe) and Zinc (Zn) Concentration in Lentils and Their Characterization under Terai Environments of Nepal","volume":"5","author":"Darai","year":"2020","journal-title":"Adv. Nutr. Food Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gupta, S., Das, S., Dikshit, H.K., Mishra, G.P., Aski, M.S., Bansal, R., Tripathi, K., Bhowmik, A., and Kumar, S. (2021). Genotype by Environment Interaction Effect on Grain Iron and Zinc Concentration of Indian and Mediterranean Lentil Genotypes. Agronomy, 11.","DOI":"10.3390\/agronomy11091761"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1002\/csc2.20675","article-title":"Evaluation of Environment and Cultivar Impact on Lentil Protein, Starch, Mineral Nutrients, and Yield","volume":"62","author":"Chen","year":"2022","journal-title":"Crop Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kole, C. (2023). Lentils (Lens Culinaris Medik): Nutritional Profile and Biofortification Prospects BT-Compendium of Crop Genome Designing for Nutraceuticals. Compendium of Crop Genome Designing for Nutraceuticals, Springer Nature Singapore.","DOI":"10.1007\/978-981-19-4169-6"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1007\/s40502-018-0411-7","article-title":"Physiological, Biochemical and Molecular Responses of Lentil (Lens Culinaris Medik.) Genotypes under Drought Stress","volume":"23","author":"Sinha","year":"2018","journal-title":"Indian J. Plant Physiol."},{"key":"ref_19","first-page":"1179","article-title":"Screening of Lentil (Lens Culinaris Medik.) Genotypes for Drought Tolerance at Seedling Stage under Different Levels of Drought Stress","volume":"2","author":"Tahir","year":"2024","journal-title":"Policy Res. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9044","DOI":"10.1021\/jf901636p","article-title":"Low Phytic Acid Lentils (Lens Culinaris L.): A Potential Solution for Increased Micronutrient Bioavailability","volume":"57","author":"Thavarajah","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"85","DOI":"10.5897\/JPBCS12.066","article-title":"Genotype\u00d7 Environment Interaction and Stability Analysis for Yield and Its Components in Lentil","volume":"5","author":"Selim","year":"2013","journal-title":"J. Plant Breed. Crop Sci"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"385","DOI":"10.5424\/sjar\/2008063-5292","article-title":"Genotype \u00d7 Environment Interaction for Grain Yield of Some Lentil Genotypes and Relationship among Univariate Stability Statistics","volume":"6","author":"Dehghani","year":"2008","journal-title":"Spanish J. Agric. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Yadav, N.K., Ghimire, S.K., Sah, B.P., Sarker, A., Shrestha, S.M., and Sah, S.K. (2016). Genotype \u00d7 Environment Interaction and Stability Analysis in Lentil (Lens Culinaris Medik.). Int. J. Environ. Agric. Biotechnol., 1.","DOI":"10.22161\/ijeab\/1.3.7"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Tziouvalekas, M., Tigka, E., Kargiotidou, A., Beslemes, D., Irakli, M., Pankou, C., Arabatzi, P., Aggelakoudi, M., Tokatlidis, I., and Mavromatis, A. (2022). Seed Yield, Crude Protein and Mineral Nutrients of Lentil Genotypes Evaluated across Diverse Environments under Organic and Conventional Farming. Plants, 11.","DOI":"10.3390\/plants11233328"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1002\/jsfa.1531","article-title":"Relationship between Physicochemical and Cooking Properties, and Effects of Cooking on Antinutrients, of Yellow Field Peas (Pisum Sativum)","volume":"83","author":"Wang","year":"2003","journal-title":"J. Sci. Food Agric."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1002\/jsfa.4683","article-title":"Micronisation and Hot Air Roasting of Cowpeas as Pretreatments to Control the Development of Hard-to-cook Phenomenon","volume":"92","author":"Ndungu","year":"2012","journal-title":"J. Sci. Food Agric."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Bansal, R., Bana, R.S., Dikshit, H.K., Srivastava, H., Priya, S., Kumar, S., Aski, M.S., Kumari, N.K.P., Gupta, S., and Kumar, S. (2023). Seed Nutritional Quality in Lentil (Lens Culinaris) under Different Moisture Regimes. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1141040"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.foodchem.2010.02.073","article-title":"Phytic Acid and Fe and Zn Concentration in Lentil (Lens Culinaris L.) Seeds Is Influenced by Temperature during Seed Filling Period","volume":"122","author":"Thavarajah","year":"2010","journal-title":"Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"150","DOI":"10.9734\/IJPSS\/2015\/14824","article-title":"Yield Stability of Newly Released Wheat Varieties in Multi-Environments of Bangladesh","volume":"6","author":"Alam","year":"2015","journal-title":"Int. J. Plant Soil Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.2135\/cropsci2011.01.0016","article-title":"Assessing the Representativeness and Repeatability of Test Locations for Genotype Evaluation","volume":"51","author":"Yan","year":"2011","journal-title":"Crop Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2316","DOI":"10.1007\/s42729-023-01182-x","article-title":"Estimation of G\u00d7 E Interaction of Lentil Genotypes for Yield Using AMMI and GGE Biplot in Pakistan","volume":"23","author":"Ghaffar","year":"2023","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Hossain, M.A., Sarker, U., Azam, M.G., Kobir, M.S., Roychowdhury, R., Ercisli, S., Ali, D., Oba, S., and Golokhvast, K.S. (2023). Integrating BLUP, AMMI, and GGE Models to Explore GE Interactions for Adaptability and Stability of Winter Lentils (Lens Culinaris Medik.). Plants, 12.","DOI":"10.3390\/plants12112079"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1002\/csc2.20282","article-title":"Genetic Stability and Genotype\u00d7 Environment Interaction Analysis for Seed Protein Content and Protein Yield of Lentil","volume":"61","author":"Subedi","year":"2021","journal-title":"Crop Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2134\/age2018.10.0051","article-title":"Yield Performance of Some Lentil Genotypes over Different Environments","volume":"2","author":"Abbas","year":"2019","journal-title":"Agrosystems, Geosci. Environ."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1080\/00103628909368129","article-title":"A Manual Colorimetric Procedure for Measuring Ammonium Nitrogen in Soil and Plant Kjeldahl Digests","volume":"20","author":"Baethgen","year":"1989","journal-title":"Commun. Soil Sci. Plant Anal."},{"key":"ref_36","unstructured":"(2023, January 10). Megazyme Phytic Acid Assay Kit. Available online: https:\/\/www.megazyme.com\/phytic-acid-assay-kit."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/13\/2019\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:02:39Z","timestamp":1760032959000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/13\/2019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":36,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["plants14132019"],"URL":"https:\/\/doi.org\/10.3390\/plants14132019","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,1]]}}}