{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T20:20:58Z","timestamp":1784319658112,"version":"3.55.0"},"reference-count":46,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development, and Innovation, Serbia","award":["451-03-136\/2025-03\/200122"],"award-info":[{"award-number":["451-03-136\/2025-03\/200122"]}]},{"name":"Ministry of Science, Technological Development, and Innovation, Serbia","award":["451-03-137\/2025-03\/200122"],"award-info":[{"award-number":["451-03-137\/2025-03\/200122"]}]},{"name":"Ministry of Science, Technological Development, and Innovation, Serbia","award":["APVV-20-0058"],"award-info":[{"award-number":["APVV-20-0058"]}]},{"name":"European Regional Development Fund","award":["451-03-136\/2025-03\/200122"],"award-info":[{"award-number":["451-03-136\/2025-03\/200122"]}]},{"name":"European Regional Development Fund","award":["451-03-137\/2025-03\/200122"],"award-info":[{"award-number":["451-03-137\/2025-03\/200122"]}]},{"name":"European Regional Development Fund","award":["APVV-20-0058"],"award-info":[{"award-number":["APVV-20-0058"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The emergence of antimicrobial resistance and the increasing demand for a healthier lifestyle have set new goals for science and industry. In the search for new, more effective, and environmentally friendly antimicrobial agents, special attention is being paid to natural resources. In this regard, essential oils derived from plants, which are widely used in the cosmetic, food, and pharmaceutical industries, are one of the solutions. In view of the above, this study aims to investigate the biological effects of Abies alba essential oil (AAEO). The chemical profile of AAEO was evaluated by GC\/MS analysis, which revealed a high abundance of limonene (52.2%) and \u03b1-pinene (36.2%). Antioxidant activity evaluation showed a higher potential of AAEO in scavenging ABTS radical species with an IC50 value of 1.18 \u00b1 0.05 mg\/mL. In vitro antimicrobial activity was determined by disc diffusion and minimum inhibitory concentration assays and showed that AAEO was more efficient in inhibiting the growth of G+ bacterial species. On contrary, in situ evaluations of antimicrobial effects of AAEO on different food models (strawberry, kiwi, white radish, and beetroot) resulted in more efficient suppression of G\u2212 bacterial species. Although AAEO showed low effects on yeasts determined by in vitro methods, in situ investigations showed its higher potential in eradication of Candida yeast. The antibiofilm properties of the AAEO matrix were determined by means of crystal violet assay and MALDI-TOF MS Biotyper analysis against biofilm-forming Salmonella enterica. The analysis performed led to the conclusion that AAEO, when applied prior to biofilm formation, may contribute to the removal of planktonic cells and alter the abiotic surface, thereby reducing the suitability of Salmonella enterica for microbial attachment.<\/jats:p>","DOI":"10.3390\/plants14132071","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T02:53:07Z","timestamp":1751856787000},"page":"2071","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antimicrobial Effects of Abies alba Essential Oil and Its Application in Food Preservation"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7222-7245","authenticated-orcid":false,"given":"Milena D.","family":"Vuki\u0107","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4382-9743","authenticated-orcid":false,"given":"Nenad L.","family":"Vukovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Radovi\u0107","family":"Jakovljevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7066-9928","authenticated-orcid":false,"given":"Marija S.","family":"Risti\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4460-0222","authenticated-orcid":false,"given":"Miroslava","family":"Ka\u010d\u00e1niov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Horticulture, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Trieda Andreja Hlinku 2, 94976 Nitra, Slovakia"},{"name":"School of Medical & Health Sciences, VIZJA University, Okopowa 59, 01043 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"29078","DOI":"10.1039\/D2RA04884A","article-title":"Antimicrobial Potentials of Natural Products against Multidrug Resistance Pathogens: A Comprehensive Review","volume":"12","author":"Elmaidomy","year":"2022","journal-title":"RSC Adv."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.2147\/IDR.S173867","article-title":"Antibiotic Resistance: A Rundown of a Global Crisis","volume":"11","author":"Aslam","year":"2018","journal-title":"Infect. 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