{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:21:35Z","timestamp":1788322895545,"version":"build-2803163510"},"reference-count":55,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060357"],"award-info":[{"award-number":["32060357"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["202505AF350074"],"award-info":[{"award-number":["202505AF350074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Academician Expert Workstation Project","award":["32060357"],"award-info":[{"award-number":["32060357"]}]},{"name":"Academician Expert Workstation Project","award":["202505AF350074"],"award-info":[{"award-number":["202505AF350074"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Aquilaria sinensis (Lour.) Gilg, the exclusive botanical source of Chinese agarwood, holds significant medicinal value. This study investigated the agarwood-inducing potential of a Fusarium strain obtained through prior isolation work. Through integrated morphological characterization and molecular phylogenetic analysis, the strain was conclusively identified as Fusarium equiseti. GC-MS analysis revealed that fungal inoculation induced the synthesis of characteristic sesquiterpenes and aromatic compounds consistent with natural agarwood profiles. Quantitative determination demonstrated progressive accumulation of agarotetrol, a key quality marker, reaching 0.034%, 0.039%, and 0.038% at 2, 4, and 6 months post-inoculation, respectively\u2014significantly exceeding levels from physical wounding (p &lt; 0.05) and PDA control treatments. Histological examination showed characteristic yellow-brown oleoresin deposits concentrated in the inner phloem, mirroring the anatomical features of wild-type agarwood. Critical quality parameters measured in December-harvested samples included ethanol extractives (17.69%), chromone derivatives 2-[2-(4-methoxyphenyl) ethyl] chromone, and 2-(2-phenylethyl) chromone (2.13%), all meeting or surpassing the specifications outlined in the National Standard for Agarwood Classification (LY\/T 3223-2020). These comprehensive findings establish F. equiseti as a promising microbial agent for sustainable agarwood production in A. sinensis plantations.<\/jats:p>","DOI":"10.3390\/plants14152272","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T16:04:51Z","timestamp":1753286691000},"page":"2272","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The First Report on Agarwood Formation of Aquilaria sinensis (Lour.) Spreng Induced by Fusarium equiseti"],"prefix":"10.3390","volume":"14","author":[{"given":"Libao","family":"Zhang","sequence":"first","affiliation":[{"name":"Yunnan Academy of Forestry and Grassland, Kunming 650201, China"},{"name":"College of Forestry, Southwest Forestry University, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3576-7514","authenticated-orcid":false,"given":"Jianglongze","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Forestry and Grassland, Kunming 650201, China"},{"name":"College of Forestry, Southwest Forestry University, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiling","family":"Yuan","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Forestry and Grassland, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Feng","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Forestry and Grassland, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Forestry and Grassland, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,23]]},"reference":[{"key":"ref_1","unstructured":"National Pharmacopoeia Commission (2020). Pharmacopoeia of the People\u2019s Republic of China (Part I), China Pharmaceutical Science and Technology Press."},{"key":"ref_2","first-page":"156","article-title":"Advances in studies on mechanism of agarwood formation in Aquilaria sinensis and its hypothesis of agarwood formation induced by defense response","volume":"41","author":"Zhang","year":"2010","journal-title":"J. Chin. Pharm. Sci."},{"key":"ref_3","first-page":"43","article-title":"Anti-inflammatory effects of the ethyl acetate extract of Aquilaria crassna inhibits LPS-induced tumour necrosis factor-alpha production by attenuating P38 MAPK activation","volume":"5","author":"Kumphune","year":"2011","journal-title":"Int. J. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1021\/np50017a010","article-title":"Plant anticancer agents. XIX Constituents of Aquilaria malaccensis","volume":"44","author":"Gunasekera","year":"1981","journal-title":"J. Nat. Prod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s11418-007-0177-0","article-title":"Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components","volume":"62","author":"Takemoto","year":"2007","journal-title":"J. Nat. Med."},{"key":"ref_6","first-page":"29","article-title":"Review for Chemical Components and Quality Assessment on Agarwood","volume":"32","author":"Shang","year":"2018","journal-title":"China Wood Industry"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s11676-013-0395-0","article-title":"Fungal inoculation induces agarwood in young Aquilaria malaccensis trees in the nursery","volume":"25","author":"Mohamed","year":"2014","journal-title":"J. For. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.phytol.2016.12.004","article-title":"Chemical solution is an efficient method to induce the formation of 2-(2-phenylethyl) chromone derivatives in Aquilaria sinensis","volume":"19","author":"Huang","year":"2017","journal-title":"Phytochem. Lett."},{"key":"ref_9","first-page":"16","article-title":"The role of fungi in the origin of oleoresin deposits of agaru in the wood of Aquilaria agallocha Roxb","volume":"6","author":"Gibson","year":"1977","journal-title":"Bano. Biggyan. Patrika."},{"key":"ref_10","first-page":"46","article-title":"Screening and Identification of the Aroma-promoting Fungi of Aquilaria sinensis(Lour.)Gilg","volume":"51","author":"Chen","year":"2023","journal-title":"Trop. For."},{"key":"ref_11","first-page":"244","article-title":"A field survey of agarwood in Indonesia","volume":"22","author":"Ueda","year":"2005","journal-title":"J. Tradit. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1039\/D0NP00042F","article-title":"Natural products in agarwood and Aquilaria plants: Chemistry, biological activities and biosynthesis","volume":"38","author":"Li","year":"2021","journal-title":"Nat. Prod. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Yang, L., Yang, J.L., Dong, W.H., Wang, Y.L., Zeng, J., Yuan, J.Z., Wang, H., Mei, W.L., and Dai, H.F. (2021). The Characteristic Fragrant Sesquiterpenes and 2-(2-Phenylethyl)chromones in Wild; Cultivated \u201cQi-Nan\u201d Agarwood. Molecules, 26.","DOI":"10.3390\/molecules26020436"},{"key":"ref_14","first-page":"38","article-title":"GC-MS Analysis of the Chemical Components of the Volatile Oil from Leaves of Aquilaria sinensis (Lour.) Gilg","volume":"2","author":"Wei","year":"2011","journal-title":"Med. Plant"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1749-8546-4-5","article-title":"GC-MS analysis of volatile oil components of agarwood from different producing areas","volume":"32","author":"Guo","year":"2009","journal-title":"Chin. Med. Mat."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105564","DOI":"10.1016\/j.fitote.2023.105564","article-title":"Eight new 2-(2-phenylethyl)chromone derivatives from agarwood of Aquilaria sinensis with anti-inflammatory activity","volume":"169","author":"Yang","year":"2023","journal-title":"Fitoterapia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1002\/rcm.6388","article-title":"Evaluating agarwood products for 2-(2-phenylethyl)chromones using direct analysis in real time time-of-flight mass spectrometry","volume":"26","author":"Lancaster","year":"2012","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_18","first-page":"6","article-title":"Study on the correlation between benzyl acetone and extract content in agarwood","volume":"35","author":"Gao","year":"2012","journal-title":"Chin. Med. Mat."},{"key":"ref_19","unstructured":"(2020). Agarwood (Sinking-Quality) Grading. National Forestry and Grassland Administration (Standard No. LY\/T 3223-2020)."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Leslie, J.F., and Summerell, B.A. (2007). The Fusarium Laboratory Manual, Blackwell Publishing.","DOI":"10.1002\/9780470278376"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1021\/acs.jnatprod.6b01085","article-title":"Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community","volume":"80","author":"Raja","year":"2017","journal-title":"J. Nat. Prod."},{"key":"ref_22","unstructured":"Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatography\/Mass Spectrometry, Allured Publishing Corporation. [4th ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0021-9673(01)80947-X","article-title":"A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography","volume":"11","author":"Kratz","year":"1963","journal-title":"J. Chromatogr. A"},{"key":"ref_24","unstructured":"Linstrom, P.J., and Mallard, W.G. (2020). NIST Chemistry WebBook, NIST Standard Reference Database Number 69, National Institute of Standards and Technology (NIST)."},{"key":"ref_25","unstructured":"(1998). Essential Oils\u2014General Guidance on Chromatographic Profiles\u2014Part 2: Utilization of Chromatographic Profiles of Samples of Essential Oils (Standard No. ISO 11024-2:1998)."},{"key":"ref_26","first-page":"10","article-title":"SPME\/GC-MS Analysis on Volatile Constituents of Agarwood Produced by Three Artificial Inducing Methods","volume":"37","author":"Jingfei","year":"2018","journal-title":"J. Instrum. Anal."},{"key":"ref_27","unstructured":"(2013). The General Method of Wood Identification (Standard No. SAC\/TC41. GB\/T 29894-2013)."},{"key":"ref_28","unstructured":"(2017). Agarwood. National Forestry and Grassland Administration (Standard No. LY\/T 2904-2017)."},{"key":"ref_29","first-page":"799","article-title":"Isolation and Identification of Secondary Metabolites from the Solid Culture of Endophytic Fungal Strain Botryosphaeria rhodina A13 from Aquilaria sinensis","volume":"27","author":"Zhang","year":"2015","journal-title":"Nat. Prod. Res. Dev."},{"key":"ref_30","first-page":"58","article-title":"2-(2-Phenylethyl)chromones from Endophytic Fungal Strain Botryosphaeria rhodina A13 from Aquilaria sinensis","volume":"9","author":"Zhang","year":"2017","journal-title":"Chin. Herb. Med."},{"key":"ref_31","first-page":"11","article-title":"Structural survey and artificial induction of aloeswood","volume":"90","author":"Tabata","year":"2003","journal-title":"Wood Res. Bull. Wood Res. Inst. Kyoto Univ."},{"key":"ref_32","first-page":"5","article-title":"Isolation of Endophytic Fungi from Aquilaria sinensis and Screening of Aquilaria sinensis Fungi","volume":"45","author":"Huang","year":"2017","journal-title":"Jiangsu Agric. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"11","DOI":"10.4103\/0973-7847.112833","article-title":"Endophytic bacteria as a source of novel antibiotics: An overview","volume":"7","author":"Christina","year":"2013","journal-title":"Pharmacogn. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Pimentel, M.R., Molina, G., Dion\u00edsio, A.P., Junior, M.R.M., and Pastore, G.M. (2011). The Use of Endophytes to Obtain Bioactive Compounds and Their Application in Biotransformation Process. Biotechnol. Res. Int., 2011.","DOI":"10.4061\/2011\/576286"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.steroids.2007.08.008","article-title":"Six new ergosterols from the marine-derived fungus Rhizopus sp.","volume":"73","author":"Wang","year":"2008","journal-title":"Steroids"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1080\/14786410902836701","article-title":"A new anthraquinone derivative from the marine endophytic fungus Fusarium sp. (No. b77)","volume":"24","author":"Shao","year":"2010","journal-title":"Nat. Prod. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1021\/np800455b","article-title":"An Endophytic Fungus from Camptotheca acuminata That Produces Camptothecin and Analogues","volume":"72","author":"Kusari","year":"2009","journal-title":"J. Nat. Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1007\/s10600-019-02915-0","article-title":"Secondary Metabolites from the Endophytic Fungus Fusarium equiseti and Their Antibacterial Activities","volume":"55","author":"Liu","year":"2019","journal-title":"Chem. Nat. Compd."},{"key":"ref_39","first-page":"125","article-title":"Isolation and Identification of Pathogenic Fungi of Carambola Fruit and Study on Their Metabolites","volume":"45","author":"Xiang","year":"2024","journal-title":"Sci. Technol. Food Ind."},{"key":"ref_40","first-page":"203","article-title":"Isolation and Identification of Pathogens of Guichang Kiwifruit Pedicle Rot and Determination of Toxicity of Control Agents","volume":"51","author":"Mi","year":"2022","journal-title":"South China Fruits"},{"key":"ref_41","first-page":"1348","article-title":"Identification of the Pathogen of Astragalus membranaceus var. mongholicus Crown Rot","volume":"46","author":"Hu","year":"2023","journal-title":"Chin. Med. Mat."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1128\/AAC.5.6.634","article-title":"Antibiotic Produced by Fusarium equiseti NRRL 5537","volume":"5","author":"Burmeister","year":"1974","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_43","first-page":"391","article-title":"Colonisation of barley roots by endophytic Fusarium equiseti and Pochonia chlamydosporia: Effects on plant growth and disease","volume":"155","author":"Rosso","year":"2010","journal-title":"Ann. Appl. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1007\/s00284-016-1193-7","article-title":"Agarwood Formation Induced by Fermentation Liquid of Lasiodiplodia theobromae, the Dominating Fungus in Wounded Wood of Aquilaria sinensis","volume":"74","author":"Chen","year":"2017","journal-title":"Curr. Microbiol."},{"key":"ref_45","first-page":"1942","article-title":"Effects of different aroma-forming methods on the formation of aromatic substances in the xylem of Aquilaria sinensis","volume":"43","author":"Pang","year":"2023","journal-title":"Acta Bot. Boreali-Occident. Sin."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s00468-018-1799-4","article-title":"Interxylary phloem and xylem rays are the structural foundation of agarwood resin formation in the stems of Aquilaria sinensis","volume":"33","author":"Liu","year":"2019","journal-title":"Trees"},{"key":"ref_47","first-page":"146","article-title":"Comparison of wood tissue structure and chemical composition of agarwood produced by two species of Aquilaria sinensis","volume":"55","author":"Wang","year":"2019","journal-title":"Sci. Silvae Sin."},{"key":"ref_48","first-page":"77","article-title":"Histology Study of Aquilaria malaccensis and the Agarwood Resin Formation under Light Microscope, DBLP","volume":"5","author":"Peng","year":"2014","journal-title":"J. Agrobiotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s13225-010-0039-z","article-title":"Fungal diversity in wounded stems of Aquilaria malaccensis","volume":"43","author":"Mohamed","year":"2010","journal-title":"Fungal Divers."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s11101-017-9492-6","article-title":"Artificial production of agarwood oil in Aquilaria sp. by fungi: A review","volume":"16","author":"Chhipa","year":"2017","journal-title":"Phytochem. Rev."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Yang, J.L., Dong, W.H., Kong, F.D., Liao, G., Wang, J., Li, W., Mei, W.L., and Dai, H.F. (2016). Characterization and analysis of 2-(2-phenylethyl)-chromone derivatives from agarwood (Aquilaria crassna) by artificial holing for different times. Molecules, 21.","DOI":"10.3390\/molecules21070911"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"114129","DOI":"10.1016\/j.indcrop.2021.114129","article-title":"The formation and quality evaluation of agarwood induced by the fungi in Aquilaria sinensis","volume":"173","author":"Ma","year":"2021","journal-title":"Ind. Crops"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3086","DOI":"10.3390\/molecules18033086","article-title":"Whole-tree agarwood-inducing technique: An efficient novel technique for producing high-quality agarwood in cultivated Aquilaria sinensis trees","volume":"18","author":"Liu","year":"2013","journal-title":"Molecules"},{"key":"ref_54","first-page":"28","article-title":"Effects of different treatments on the growth and agarwood formation of Aquilaria sinensis","volume":"43","author":"Wang","year":"2023","journal-title":"Soc. Sci."},{"key":"ref_55","first-page":"23","article-title":"Quality Analysis of Three Types of Artificially Induced Agarwood","volume":"50","author":"Ye","year":"2023","journal-title":"J. Fujian Coll. For."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/15\/2272\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:14:53Z","timestamp":1760033693000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/15\/2272"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,23]]},"references-count":55,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["plants14152272"],"URL":"https:\/\/doi.org\/10.3390\/plants14152272","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,23]]}}}