{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:38:37Z","timestamp":1760060317391,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,17]],"date-time":"2025-08-17T00:00:00Z","timestamp":1755388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Establishment of a specialized production cluster for Platycodon grandiflorus and development of high-functionality product technology","doi-asserted-by":"publisher","award":["RS-2025-14383142"],"award-info":[{"award-number":["RS-2025-14383142"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Sambucus williamsii Hance (Viburnaceae), the Korean elderberry, is widely used in herbal medicine and in the food industry. It is known to have various pharmacological effects, including antitumor, antioxidant, anti-inflammatory, and antimicrobial activities. During our search for anti-Helicobacter pylori compounds from natural resources, the methanol extract of the S. williamsii branch significantly inhibited the growth of H. pylori. Three phenolic and four lignan compounds were isolated from the methylene chloride fraction that had shown the most potent anti-H. pylori activity among the hexane, methylene chloride, ethyl acetate, butanol, and water fractions. The chemical structures were identified to be three phenolics of sylvopinol (1), dihydroconiferyl alcohol (2), and (7S,8R)-guaiacylglycerol (3) and four lignans of boehmenan (4), (7S,8S)-guaiacylglycerol \u03b2-coniferyl ether (6) and lawsonicin (7) with a new lignan, (7R,8R)-sambucanol (5), the structure of which was established by 1H- and 13C-NMR, and HRESI-MS, as well as quantum chemical electronic circular dichroism (ECD) calculations. Among the isolates, compounds 3 and 4 exhibited significant anti-H. pylori activity against strains 51 and 26695. Compound 3 displayed more potent antibacterial activity with MIC values of 3.13 and 6.25 \u03bcM, and MIC50 values of 28.5 and 56.8 \u03bcM against the two strains, respectively. Their inhibitory activities were higher than those of a positive control, quercetin. Furthermore, these two compounds showed moderate urease inhibitory activity. A molecular docking simulation revealed the high binding ability of 3 and 4 to the active site of H. pylori urease. These results will provide further insights into the design of more potent natural products for eradicating H. pylori.<\/jats:p>","DOI":"10.3390\/plants14162558","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T07:53:12Z","timestamp":1755503592000},"page":"2558","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti-Helicobacter pylori Compounds of Sambucus\u00a0williamsii Hance Branch"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1711-0210","authenticated-orcid":false,"given":"Woo-Jin","family":"Jeong","sequence":"first","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3190-792X","authenticated-orcid":false,"given":"Dong-Min","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1450-5635","authenticated-orcid":false,"given":"Atif Ali Khan","family":"Khalil","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9179-3998","authenticated-orcid":false,"given":"Bashu Dev","family":"Neupane","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8899-5310","authenticated-orcid":false,"given":"Seong-Joon","family":"Cho","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na-In","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5285-9138","authenticated-orcid":false,"given":"Ki-Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1201-0746","authenticated-orcid":false,"given":"Mi-Jeong","family":"Ahn","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,17]]},"reference":[{"key":"ref_1","first-page":"209","article-title":"Global cancer statistics 2020: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. 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