{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:09:38Z","timestamp":1772860178603,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Larch-Supported Research and Development","award":["20201215"],"award-info":[{"award-number":["20201215"]}]},{"name":"Larch-Supported Research and Development","award":["2023YFD2200604"],"award-info":[{"award-number":["2023YFD2200604"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["20201215"],"award-info":[{"award-number":["20201215"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2200604"],"award-info":[{"award-number":["2023YFD2200604"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Hypolepis punctata, an aromatic fern with insect-resistant and ornamental potential. Up to date, no studies have reported its micropropagation, particularly using vegetative organs as explants. The optimized stolon sterilization (81.11%) employed 75% ethanol (30 s) and 15% sodium hypochlorite (12 min). The optimal conditions for GGB induction (75.56%) and proliferation (8.46 mm) were achieved using Murashige and Skoog (MS) medium + 2.0 mg\/L 6-benzylaminopurine (BA) + 0.2 mg\/L 1-naphthaleneacetic acid (NAA). The optimal plant growth regulator (PGR) formula for sporophyte regeneration was 0.5 mg\/L BA + 0.1 mg\/L NAA + 2 g\/L activated charcoal (AC), achieving a 98.89% induction rate and 49.19 buds per explant. The 1\/4 MS medium had the greatest promoting effect on biomass accumulation and leaf expansion. Optimal shoot elongation (97.78% success, 4.83 cm) was achieved in 1\/4 MS + 0.5 mg\/L BA + 0.1 mg\/L NAA + 2 g\/L AC, and optimized rooting (92.22%) was achieved using 1\/4 MS + 0.5 mg\/L indole-3-butyric acid (IBA) + 0.1 mg\/L NAA + 2 g\/L AC, producing 25.27 roots per plantlet. Crucially, ISSR analysis confirmed the genetic stability of all regenerants. This optimized protocol establishes a scalable micropropagation system, enhancing both commercial cultivation and genetic improvement potential in Hypolepis punctata.<\/jats:p>","DOI":"10.3390\/plants14162569","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T13:28:22Z","timestamp":1755523702000},"page":"2569","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Efficient In Vitro Plantlet Regeneration from Stolon Explants and Genetic Stability Assessment Using ISSR Markers in the Ornamental Fern Hypolepis punctata"],"prefix":"10.3390","volume":"14","author":[{"given":"Xinyuan","family":"Wang","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China"},{"name":"CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China"},{"name":"School of Life Sciences, Jishou University, Jishou 416000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuetong","family":"Yan","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7257-8763","authenticated-orcid":false,"given":"Keyuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China"},{"name":"CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Shen","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Shanghai Normal University, Shanghai 200223, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9661-3746","authenticated-orcid":false,"given":"Qiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jishou University, Jishou 416000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1000-4254","authenticated-orcid":false,"given":"Mulan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China"},{"name":"CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"ref_1","first-page":"227","article-title":"A review of the fern genus Hypolepis (Dennstardtiaceae) in the Malesian and Pacific regions","volume":"32","author":"Brownsey","year":"1987","journal-title":"Blumea Biodivers. Evol. Biogeogr. Plants"},{"key":"ref_2","unstructured":"Wu, Z., Raven, P.H., and Hong, D. (2013). Hypolepis. Flora of China, Vols. 2\u20133, Missouri Botanical Garden Press."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"106881","DOI":"10.1016\/j.ympev.2020.106881","article-title":"New insights into the evolution of the fern family Dennstaedtiaceae from an expanded molecular phylogeny and morphological analysis","volume":"150","author":"Schwartsburd","year":"2020","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_4","first-page":"15","article-title":"Characteristics of intragametophytic selfing of ornamental Ferns Hypolepis punctata on sporophyte germination and development","volume":"44","author":"Li","year":"2024","journal-title":"Hortic. Seed"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Huang, K., Shang, H., Zhou, Q., Wang, Y., Shen, H., and Yan, Y. (2021). Volatiles Induced from Hypolepis punctata (Dennstaedtiaceae) by Herbivores Attract Sclomina erinacea (Hemiptera: Reduviidae): Clear Evidence of Indirect Defense in Fern. Insects, 12.","DOI":"10.3390\/insects12110978"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1054\/bjoc.2000.1368","article-title":"Carcinogenic effects of ptaquiloside in bracken fern and related compounds","volume":"83","author":"Potter","year":"2000","journal-title":"Br. J. Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1246\/cl.1972.375","article-title":"Structures and Syntheses of Hypolepin A, B and C, Sesquiterpenes from Hypolepis punctata Mett","volume":"5","author":"Hayashi","year":"1972","journal-title":"Chem. Lett."},{"key":"ref_8","unstructured":"Lai, K. (2003). Studies on the Cytotoxic Principles from Hypolepis punctata. [Master\u2019s Thesis, Taipei Medical University]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s11627-010-9303-x","article-title":"Conservation in vitro of rare and threatened ferns\u2014Case studies of biodiversity hotspot and island species","volume":"47","author":"Barnicoat","year":"2011","journal-title":"In Vitro Cell. Dev. Biol.-Plant"},{"key":"ref_10","first-page":"345","article-title":"Observation on gametophyte development of Hypolepis punctata","volume":"3","author":"Liu","year":"2012","journal-title":"J. Trop. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.scienta.2012.03.017","article-title":"Improved micropropagation protocol for leatherleaf fern (Rumohra adiantiformis) using rhizomes as donor explant","volume":"140","author":"Winarto","year":"2012","journal-title":"Sci. Hortic."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"112598","DOI":"10.1016\/j.scienta.2023.112598","article-title":"Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system","volume":"324","author":"Lin","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_13","first-page":"1839","article-title":"Research Advances in Tissue Culture of Ornamental Ferns","volume":"39","author":"Li","year":"2012","journal-title":"Acta Hortic. Sin."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s11240-022-02402-5","article-title":"In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis","volume":"152","author":"Pu","year":"2023","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1022650701341","article-title":"In vitro culture of ornamental ferns","volume":"73","author":"Revilla","year":"2003","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1079\/IVP2006810","article-title":"Callus culture and an unconventional pattern of sporophyte regeneration in Drynaria quercifolia\u2014A medicinal fern","volume":"42","author":"Hegde","year":"2006","journal-title":"Vitr. Cell. Dev. Biol.-Plant"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1086\/336227","article-title":"Physiological and morphogenetic studies of fern gametophytes in aseptic culture. I. Callus tissues from dark-cultured Pteris vittata","volume":"124","author":"Kato","year":"1963","journal-title":"Bot. Gaz."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7488","DOI":"10.1007\/s00344-023-11026-8","article-title":"Gametophyte development and sporophyte regeneration of Alsophila spinulosa","volume":"42","author":"Xiong","year":"2023","journal-title":"J. Plant Growth Regul."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0304-4238(94)90007-8","article-title":"Plant regeneration from leaf explants of the fern Platycerium bifurcatum in vitro","volume":"56","author":"Camloha","year":"1994","journal-title":"Sci. Hortic."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1023\/A:1006348628114","article-title":"In vitro organogenesis of Polypodium cambricum","volume":"57","author":"Bertrand","year":"1999","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0304-4238(87)90021-5","article-title":"In vitro propagation of Nephrolepis cordifolia Prsel","volume":"32","author":"Higuchi","year":"1987","journal-title":"Sci. Hortic."},{"key":"ref_22","first-page":"455","article-title":"In vitro propagation of Platycerium bifurcatum (cav.) c. chr. via green globular body initiation","volume":"52","author":"Liao","year":"2011","journal-title":"Bot. Stud."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"uhae292","DOI":"10.1093\/hr\/uhae292","article-title":"Tissue culture-independent approaches to revolutionizing plant transformation and gene editing","volume":"12","author":"Quiroz","year":"2025","journal-title":"Hortic. Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Bui, L.T., Long, H., Irish, E.E., Cordle, A.R., and Cheng, C.L. (2018). The power of gametophyte transformation. Current Advances in Fern Research, Springer International Publishing.","DOI":"10.1007\/978-3-319-75103-0_13"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Xiang, D.L., and Li, G.S. (2024). Control of leaf development in the water fern Ceratopteris richardii by the auxin efflux transporter CrPINMa in the CRISPR\/Cas9 analysis. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-05009-4"},{"key":"ref_26","first-page":"495","article-title":"Overview of gene function research in monilophytes (ferns)","volume":"59","author":"Wang","year":"2024","journal-title":"J. Integr. Plant Biol."},{"key":"ref_27","first-page":"100013","article-title":"Unveiling novel genes in fern genomes for the design of stress tolerant crops","volume":"1","author":"Chen","year":"2022","journal-title":"Crop Des."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1080\/15476286.2016.1191728","article-title":"Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects","volume":"13","author":"Shukla","year":"2016","journal-title":"RNA Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A revised medium for rapid growth and bio assays with tobacco tissue cultures","volume":"15","author":"Murashige","year":"1962","journal-title":"Physiol. Plant."},{"key":"ref_30","unstructured":"Chu, C.C. (1981). The N6 medium and its applications to anther culture of cereal crops. Proc. Symp. Plant Tissue Cult., 43\u201350."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF00269282","article-title":"Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)","volume":"4","author":"Gupta","year":"1985","journal-title":"Plant Cell Rep."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"George, E.F., Hall, M.A., and De Klerk, G.J. (2007). Plant Propagation by Tissue Culture: Volume 1. The Background (Vol. 1), Springer Science & Business Media.","DOI":"10.1007\/978-1-4020-5005-3_1"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s11627-020-10059-y","article-title":"In vitro regeneration of the colorful fern Pteris aspericaulis var. tricolor via green globular bodies system","volume":"57","author":"Yu","year":"2021","journal-title":"Vitr. Cell. Dev. Biol.-Plant"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s11240-015-0850-z","article-title":"An unique system of somatic embryogenesis in the tree fern Cyathea delgadii Sternb.: The importance of explant type, and physical and chemical factors","volume":"123","author":"Grzyb","year":"2015","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/BF00043434","article-title":"Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis","volume":"45","author":"Bertrand","year":"1996","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s002990050557","article-title":"Rapid in vitro propagation of Matteuccia struthiopteris (L.) Todaro\u2013an edible fern","volume":"18","author":"Thakur","year":"1998","journal-title":"Plant Cell Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0304-4238(89)90146-5","article-title":"Effects of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propagation","volume":"37","author":"Higuchi","year":"1989","journal-title":"Sci. Hortic."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s11240-016-1116-0","article-title":"In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies","volume":"128","author":"Yu","year":"2017","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_39","first-page":"237","article-title":"A possible propagation system of Nephrolepis, Asplenium, Pteris, Adiantum and Rumohra (Arachniodes) through tissue culture","volume":"300","author":"Amaki","year":"1990","journal-title":"Vitr. Cult. XXIII IHC"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"C\u00e1rdenas-Aquino, M.D.R., Camas-Reyes, A., Valencia-Lozano, E., L\u00f3pez-S\u00e1nchez, L., Mart\u00ednez-Antonio, A., and Cabrera-Ponce, J.L. (2023). The Cytokinins BAP and 2-iP modulate different molecular mechanisms on shoot proliferation and Root Development in Lemongrass (Cymbopogon citratus). Plants, 12.","DOI":"10.3390\/plants12203637"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1023\/A:1006119015972","article-title":"The use of charcoal in in vitro culture\u2013A review","volume":"26","author":"Pan","year":"1998","journal-title":"Plant Growth Regul."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.biotechadv.2008.08.003","article-title":"The role of activated charcoal in plant tissue culture","volume":"26","author":"Thomas","year":"2008","journal-title":"Biotechnol. Adv."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s002990050356","article-title":"Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum","volume":"17","author":"Teng","year":"1997","journal-title":"Plant Cell Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF00272634","article-title":"Propagation of fern (Nephrolepis) through tissue culture","volume":"1","author":"Padhya","year":"1982","journal-title":"Plant Cell Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1007\/s11627-020-10055-2","article-title":"Practical methodology for gametophyte proliferation and sporophyte production in green penny fern (Lemmaphyllum microphyllum C. Presl) using mechanical fragmentation","volume":"56","author":"Jang","year":"2020","journal-title":"Vitr. Cell. Dev. Biol.-Plant"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Yan, X., Zheng, K., Li, P., Zhong, X., Zhu, Z., Zhou, H., and Zhu, M. (2024). An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers. Front. Plant Sci., 15.","DOI":"10.3389\/fpls.2024.1259925"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.scienta.2018.09.066","article-title":"A procedure for indirect shoot organogenesis of Polianthes tuberosa L. and analysis of genetic stability using ISSR markers in regenerated plants","volume":"244","author":"Nalousi","year":"2019","journal-title":"Sci. Hortic."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Zhou, H., Sun, J., Zheng, K., Zhang, X., Yao, Y., and Zhu, M. (2024). Efficient Plantlet Regeneration from Branches in Mangifera indica L. Plants, 13.","DOI":"10.3390\/plants13182595"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1500047","DOI":"10.3732\/apps.1500047","article-title":"Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)","volume":"3","author":"Shang","year":"2015","journal-title":"Appl. Plant Sci."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/16\/2569\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:30:10Z","timestamp":1760034610000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/16\/2569"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,18]]},"references-count":49,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["plants14162569"],"URL":"https:\/\/doi.org\/10.3390\/plants14162569","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,18]]}}}