{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T13:15:38Z","timestamp":1785762938452,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Aragol Baft Negin Tabarestan","award":["1542"],"award-info":[{"award-number":["1542"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Rosa canina is one of the precious native rose rootstocks with a high reputation among plant producers, which has potential horticultural and pharmacological properties related to the cosmetic values and the production of secondary metabolites. Due to high horticultural consumption, applying the plant tissue culture technique as a major tool for healthy and massive-scale production of R. canina plants is not unexpected. However, the response of R. canina in vitro plantlets to various plant tissue culture ingredients is not well understood to tender an efficient applied protocol for qualitative and quantitative in vitro propagation. In this regard, the main objective of this study is to investigate the influence of several abiotic in vitro variants including six plant tissue culture media formulations (McCown\u2019s Woody Plant Medium (WPM), Murashige and Skoog (MS), Van der Salm (VS), Schenk and Hildebrant (SH), Driver Kuniyuki Walnut (DKW), and Gamburg B5 (B5)) in combination with four concentrations (0, 1.5, 3, 4 mgL\u22121) of two types of cytokinins (6-Benzyaminopurine (BAP) and Kinetin (Kin)) simultaneously. Notably, it is perceived that DKW culture medium containing 1.5 mgL\u22121 BAP and 0.1 mgL\u22121 NAA is the best treatment for both in vitro morphological and flowering properties.<\/jats:p>","DOI":"10.3390\/plants14162606","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T15:19:02Z","timestamp":1755789542000},"page":"2606","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Superior In Vitro Responses of a Native Rose Genotype to Driver Kuniyuki Walnut (DKW) Medium in a Comparative Study Using Natural and Synthetic Plant Growth Regulators"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4303-170X","authenticated-orcid":false,"given":"Mahboubeh","family":"Davoudi Pahnekolayi","sequence":"first","affiliation":[{"name":"Department of Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universit\u00e4t Wien (TU Wien), 1040 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Parchianloo","sequence":"additional","affiliation":[{"name":"Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan M9PX+FX7, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Babouyehdarabi","sequence":"additional","affiliation":[{"name":"Department of Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universit\u00e4t Wien (TU Wien), 1040 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meysam","family":"Ghasemi","sequence":"additional","affiliation":[{"name":"Department of Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universit\u00e4t Wien (TU Wien), 1040 Vienna, Austria"},{"name":"Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan M9PX+FX7, Iran"},{"name":"Department of Plant and Environmental Sciences, Section for Crop Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"ref_1","first-page":"61","article-title":"The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.)","volume":"3","author":"Samiei","year":"2015","journal-title":"J. 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