{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T02:59:31Z","timestamp":1789268371244,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T00:00:00Z","timestamp":1756944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province (Yangzhou University)","award":["SJCX21_1615"],"award-info":[{"award-number":["SJCX21_1615"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Sand pear is a fruit tree crop with high economic value, widely cultivated in East Asia. However, ripening fruits often suffer from high-temperature stress, which has adverse effects on the quality and yield of the fruit. In this study, we perform high-temperature treatment on mature stage \u2018Housui\u2019 pear fruits. The results showed that heat stress decreased fruit firmness and mineral elements, as well as lead to the flesh appearance of watercore. High temperature induces H2O2, MDA, and the antioxidant enzyme activity including SOD, APX, POD, and CAT were significantly increased. Transcriptome and metabolomic analyses revealed that heat stress up-regulated genes related to sucrose synthesis (SPS) while down-regulating those involved in sucrose degradation (SS and NI), resulting in sucrose accumulation. Moreover, the expression of sorbitol dehydrogenase (SDH) and sorbitol transporter (SOT) genes was markedly suppressed, leading to sorbitol accumulation and impaired transport, which promoted watercore development. High temperature also stimulated the expression of ethylene synthesis genes, accelerating abnormal ripening of fruits. In addition, high temperature decreased the accumulation of organic acid and bioactive compounds. Additionally, several antioxidant enzymes genes, five heat shock transcription factors (HSFs) and 34 heat shock protein (HSP) genes were significantly up-regulated. Together, these findings provided new insights into the transcriptional response and metabolomic reprogramming of sand pear response to high-temperature stress.<\/jats:p>","DOI":"10.3390\/plants14172776","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T15:31:21Z","timestamp":1756999881000},"page":"2776","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integrated Transcriptome and Metabolome Analysis of Mature Stage Sand Pear Fruit Response to High-Temperature Stress"],"prefix":"10.3390","volume":"14","author":[{"given":"Yu-Xuan","family":"Li","sequence":"first","affiliation":[{"name":"College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Bei","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s00425-023-04320-y","article-title":"A review of changes at the phenotypic, physiological, biochemical, and molecular levels of plants due to high temperatures","volume":"259","author":"Wang","year":"2024","journal-title":"Planta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1111\/pce.12417","article-title":"Responses of tree species to heat waves and extreme heat events","volume":"38","author":"Teskey","year":"2015","journal-title":"Plant Cell Environ."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Li, M.Y., Li, J., Zhang, R., Lin, Y.X., Xiong, A.S., Tan, G.F., Luo, Y., Zhang, Y., Chen, Q., and Wang, Y. (2022). Combined analysis of the metabolome and transcriptome to explore heat stress responses and adaptation mechanisms in celery (Apium graveolens L.). Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23063367"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3780","DOI":"10.1093\/jxb\/eraa034","article-title":"Molecular and genetic bases of heat stress responses in crop plants and breeding for increased resilience and productivity","volume":"71","author":"Janni","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kopeck\u00e1, R., Kameniarov\u00e1, M., Cerny, M., Brzobohaty, B., and Nov\u00e1k, J. (2023). Abiotic stress in crop production. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24076603"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1007\/s00299-021-02696-3","article-title":"Molecular mechanisms of plant tolerance to heat stress: Current landscape and future perspectives","volume":"40","author":"Haider","year":"2021","journal-title":"Plant Cell Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1080\/07352689.2012.696453","article-title":"Sunburn of apple fruit: Historical background, recent advances and future perspectives","volume":"31","author":"Racsko","year":"2012","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e12845","DOI":"10.1111\/jtxs.12845","article-title":"Investigation on the environmental causes of tomato fruit cracking and its propagation prediction in greenhouse","volume":"55","author":"Liu","year":"2024","journal-title":"J. Texture Stud."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Do, V., Lee, Y., Park, J., Win, N.M., Kwon, S.I., Yang, S., and Kim, S. (2024). Heat stress and water irrigation management effects on the fruit color and quality of \u2018Hongro\u2019 apples. Agriculture, 14.","DOI":"10.3390\/agriculture14050761"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.scienta.2014.05.033","article-title":"Watercore disorder in Japanese pear \u2018Niitaka\u2019 is increased by high fruit temperatures during fruit maturation","volume":"175","author":"Hayama","year":"2014","journal-title":"Sci. Hortic."},{"key":"ref_11","first-page":"595","article-title":"Relationship between the temperature characteristics and the occurrence of watercore at various altitudes in \u2018Hongro\u2019 and \u2018Fuji\u2019 apples","volume":"41","author":"Kim","year":"2023","journal-title":"Hortic. Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100657","DOI":"10.1016\/j.stress.2024.100657","article-title":"Comparative transcriptome analysis of B. oleracea L. var. italica and B. macrocarpa Guss. genotypes under drought stress: De novo vs reference genome assembly","volume":"14","author":"Privitera","year":"2024","journal-title":"Plant Stress"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1007\/s11738-016-2269-5","article-title":"De novo transcriptome sequencing of pakchoi (Brassica rapa L. chinensis) reveals the key genes related to the response of heat stress","volume":"38","author":"Xu","year":"2016","journal-title":"Acta Physiol. Plant."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1111\/tpj.16612","article-title":"Complex plant responses to drought and heat stress under climate change","volume":"117","author":"Sato","year":"2024","journal-title":"Plant J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"113611","DOI":"10.1016\/j.scienta.2024.113611","article-title":"Changes in the harvesting window and quality of apple fruit cultivated under long-term high temperature and CO2","volume":"338","author":"Hassan","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sun, M.M., Khan, A., Wang, J.H., Ding, L.C., Yang, X.H., Xiong, J., Sun, Z.Y., Feng, N.J., and Zheng, D.F. (2024). Exogenous hemin increases the yield, phenolic compound content, and antioxidant enzyme activity of dragon fruit during the high-temperature period. Agronomy, 14.","DOI":"10.3390\/agronomy14081850"},{"key":"ref_17","first-page":"2033","article-title":"Response of tomato sugar and acid metabolism and fruit quality under different high temperature and relative humidity vonditions","volume":"91","author":"Zheng","year":"2022","journal-title":"Phyton-Int. J. Exp. Bot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"443","DOI":"10.21273\/JASHS.138.6.443","article-title":"High temperature alters sorbitol metabolism in Pyrus pyrifolia leaves and fruit flesh during late stages of fruit enlargement","volume":"138","author":"Liu","year":"2013","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_19","first-page":"2380","article-title":"Ethylene control of fruit fipening: Revisiting the complex network of transcriptional regulation","volume":"169","author":"Liu","year":"2015","journal-title":"Plant Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1002\/jsfa.4226","article-title":"Expression of genes associated with ethylene-signalling pathway in harvested banana fruit in response to temperature and 1-MCP treatment","volume":"91","author":"Yan","year":"2011","journal-title":"J. Sci. Food Agric."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"112243","DOI":"10.1016\/j.plantsci.2024.112243","article-title":"Abscisic acid and ethylene coordinating fruit ripening under abiotic stress","volume":"349","author":"Bianchetti","year":"2024","journal-title":"Plant Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Tan, Y., Wen, B.B., Xu, L., Zong, X.J., Sun, Y.G., Wei, G.Q., and Wei, H.R. (2023). High temperature inhibited the accumulation of anthocyanin by promoting ABA catabolism in sweet cherry fruits. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1079292"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.postharvbio.2008.05.019","article-title":"Metabolic profiling of \u2018Conference\u2019 pears under low oxygen stress","volume":"51","author":"Pedreschi","year":"2009","journal-title":"Postharvest Biol. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zhang, X.Y., Li, Y.M., Li, Y., Zhao, J.L., Cheng, Y.D., Wang, Y.X., and Guan, J.F. (2023). Changes of bioactive components and antioxidant capacity of pear ferment in simulated gastrointestinal digestion in vitro. Foods, 12.","DOI":"10.3390\/foods12061211"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"109955","DOI":"10.1016\/j.jfoodeng.2020.109955","article-title":"Quantitative detection of apple watercore and soluble solids content by near infrared transmittance spectroscopy","volume":"279","author":"Guo","year":"2020","journal-title":"J. Food Eng."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.1080\/02648725.2022.2143317","article-title":"Functional and biotechnological cues of potassium homeostasis for stress tolerance and plant development","volume":"40","author":"Kaniganti","year":"2024","journal-title":"Biotechnol. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1007\/s00425-024-04544-6","article-title":"High-temperature stress in strawberry: Understanding physiological, biochemical and molecular responses","volume":"260","author":"Ullah","year":"2024","journal-title":"Planta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/s10725-022-00889-8","article-title":"The impact of elevated CO2 concentration on fruit size, quality, and mineral nutrient composition in tomato varies with temperature regimen during growing season","volume":"100","author":"Pimenta","year":"2023","journal-title":"Plant Growth Regul."},{"key":"ref_29","first-page":"127","article-title":"Watercore in Fruits","volume":"Volume 10","author":"Kanayama","year":"2015","journal-title":"Abiotic Stress Biology in Horticultural Plants"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"261","DOI":"10.21273\/JASHS.130.2.261","article-title":"Sorbitol transporter expression in apple sink tissues: Implications for fruit sugar accumulation and watercore development","volume":"130","author":"Gao","year":"2005","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.scienta.2018.09.019","article-title":"Expression analysis of sorbitol transporters in pear tissues reveals that PbSOT6\/20 is associated with sorbitol accumulation in pear fruits","volume":"243","author":"Yu","year":"2019","journal-title":"Sci. Hortic."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1880","DOI":"10.1104\/pp.109.141374","article-title":"Apple Sucrose transporter SUT1 and sorbitol transporter SOT6 interact with cytochrome b5 to regulate their affinity for substrate sugars","volume":"150","author":"Fan","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"111179","DOI":"10.1016\/j.plantsci.2022.111179","article-title":"A sorbitol transporter gene plays specific role in the occurrence of watercore by modulating the level of intercellular sorbitol in pear","volume":"317","author":"Liu","year":"2022","journal-title":"Plant Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cukrov, D. (2018). Progress toward understanding the molecular basis of fruit response to hypoxia. Plants, 7.","DOI":"10.3390\/plants7040078"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Liu, X., Liu, D.H., Chen, T., Zhang, J., and Wang, C.L. (2022). Watercore pear fruit respiration changed and accumulated \u03b3-aminobutyric acid (GABA) in response to inner hypoxia stress. Genes, 13.","DOI":"10.3390\/genes13060977"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Li, N., Euring, D.J., Cha, J.Y., Lin, Z., Lu, M.Z., Huang, L.J., and Kim, W.Y. (2021). Plant hormone-mediated regulation of heat tolerance in response to global climate change. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.627969"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1105\/tpc.15.00435","article-title":"The transcriptional cascade in the heat stress response of arabidopsis is strictly regulated at the level of transcription factor expression","volume":"28","author":"Ohama","year":"2016","journal-title":"Plant Cell"},{"key":"ref_38","unstructured":"Cao, J.K., Jiang, W.B., and Zhao, Y.M. (2013). Experiment Guidance of Postharvest Physiology and Biochemistry of Fruits and Vegetables, China Light Industry Press."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e15328","DOI":"10.7717\/peerj.15328","article-title":"A comparison of the mineral element content of 70 different varieties of pear fruit (Pyrus ussuriensis) in China","volume":"11","author":"Liu","year":"2023","journal-title":"Peer J."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/17\/2776\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:39:39Z","timestamp":1760035179000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/17\/2776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,4]]},"references-count":39,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["plants14172776"],"URL":"https:\/\/doi.org\/10.3390\/plants14172776","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,4]]}}}