{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:46:29Z","timestamp":1760060789107,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPERJ","award":["E-26\/200.512\/2023","E-26\/211.315\/2021","310782\/2022-8"],"award-info":[{"award-number":["E-26\/200.512\/2023","E-26\/211.315\/2021","310782\/2022-8"]}]},{"name":"CAPES (Coordena\u00e7\u00e3o de Aperfeicoamento de Pessoal de N\u00edvel Superior)","award":["E-26\/200.512\/2023","E-26\/211.315\/2021","310782\/2022-8"],"award-info":[{"award-number":["E-26\/200.512\/2023","E-26\/211.315\/2021","310782\/2022-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Eperua oleifera Ducke (Fabaceae), commonly known as copa\u00edba-jacar\u00e9, is traditionally used for therapeutic purposes, like Copaifera oleoresins. Previous GC-MS studies reported its chemical composition as mainly composed of diterpenic acids, consistent with species of the same genus. Although GC-MS remains widely used for comparing compound retention times and fragmentation patterns, its application to diterpenic acids requires a derivatization step to form methyl esters due to the poor chromatographic performance of carboxylic acids on methyl silicone stationary phases. This step may lead to misinterpretations, especially considering recent findings of naturally occurring methyl esters in oleoresins that may co-elute with derivatized acids. This study aimed to apply more sensitive analytical techniques to identify both target and untargeted compounds. The resin of E. oleifera was analyzed by GC-MS to assess the presence of volatile components. Additionally, UHPLC-HRMS was employed using full-scan MS, data-dependent acquisition (DDA), and parallel reaction monitoring (PRM) in both positive and negative ESI modes. GC-MS confirmed the absence of volatile sesquiterpenes, classifying E. oleifera as a resin. Targeted UHPLC-HRMS detected natural methyl esters of diterpenic acids, while untargeted analysis using Compound Discoverer 3.3 software revealed flavonoids and phenolic compounds not previously reported. These findings support the application of UHPLC-HRMS as a powerful tool in phytochemical studies.<\/jats:p>","DOI":"10.3390\/plants14182893","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T09:32:32Z","timestamp":1758187952000},"page":"2893","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advancing the Chemical Characterization of Eperua oleifera Duke Oleoresin: A UHPLC-HRMS-Based Approach"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-3530","authenticated-orcid":false,"given":"Rayssa","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Military Institute of Engineering, Pra\u00e7a General Tib\u00farcio, 80, Praia Vermelha, Urca, Rio de Janeiro 22290-270, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1299-4121","authenticated-orcid":false,"given":"Gabriel Reis Alves","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Brazilian Doping Control Laboratory (LBCD), Chemistry Institute (IQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1755-9972","authenticated-orcid":false,"given":"Gustavo Ramalho Cardoso dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Brazilian Doping Control Laboratory (LBCD), Chemistry Institute (IQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6076-1616","authenticated-orcid":false,"given":"M\u00e1rcio Vin\u00edcius da Silva","family":"Gomes","sequence":"additional","affiliation":[{"name":"Brazilian Doping Control Laboratory (LBCD), Chemistry Institute (IQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8597-416X","authenticated-orcid":false,"given":"Henrique Marcelo Gualberto","family":"Pereira","sequence":"additional","affiliation":[{"name":"Brazilian Doping Control Laboratory (LBCD), Chemistry Institute (IQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica Costa","family":"Padilha","sequence":"additional","affiliation":[{"name":"Brazilian Doping Control Laboratory (LBCD), Chemistry Institute (IQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1365-7602","authenticated-orcid":false,"given":"Valdir F.","family":"Veiga-Junior","sequence":"additional","affiliation":[{"name":"Military Institute of Engineering, Pra\u00e7a General Tib\u00farcio, 80, Praia Vermelha, Urca, Rio de Janeiro 22290-270, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"ref_1","unstructured":"Jardim Bot\u00e2nico do Rio de Janeiro (2025, September 07). 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