{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:21:37Z","timestamp":1790011297732,"version":"4.0.1"},"reference-count":66,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Aborn commander Scientist Project of Qinghai Province","award":["2023-NK-146"],"award-info":[{"award-number":["2023-NK-146"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>This study aimed to elucidate the physiological, biochemical, and transcriptional regulatory responses of potato plants to iron deficiency stress. Two potato varieties were selected for analysis: 05P (high tuber iron content) and CI5 (low tuber iron content). Tissue culture seedlings of both varieties were subjected to iron deficiency, and the effects on stem length, root length, fresh weight, soluble sugar and protein contents, as well as the activities of superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), and leaf chlorophyll content (SPAD) values were evaluated. Additionally, the impact of iron deficiency on zinc (Zn), magnesium (Mg), calcium (Ca), manganese (Mn), and copper (Cu) concentrations in different tissues were analyzed. Transcriptomic sequencing and quantitative real-time PCR (qRT-PCR) were performed on various seedling tissues. The results showed that iron deficiency significantly inhibited seedling growth and development, resulting in reduced plant height and fresh weight, increased root length, decreased leaf SPAD content, and elevated soluble sugar and protein concentration. SOD, POD, and MDA activities were also significantly increased. Elemental analysis revealed that iron deficiency enhanced the uptake and accumulation of Zn, Mg, Ca, Mn, and Cu across different tissues. Transcriptomic analysis identified differentially expressed genes (DEGs) significantly enriched in pathways related to photosynthesis, carbon metabolism, and ribosome function in roots, stems, and leaves. Iron deficiency induced the upregulation of H+-ATPase genes in roots (PGSC0003DMG400004101, PGSC0003DMG400033034), acidifying the rhizosphere to increase active iron availability. Subsequently, this was followed by the upregulation of FRO genes (PGSC0003DMG400000184, PGSC0003DMG400010125, PGSC0003DMG401009494, PGSC0003DMG401018223), which reduce Fe3+ to Fe2+, and activation of IRT genes, facilitating Fe2+ transport to various tissues. Iron deficiency also reduced SPAD content in leaves, negatively impacting photosynthesis and overall plant growth. In response, the osmotic regulation and antioxidant defense systems were activated, enabling the plant to mitigate iron deficiency stress. Additionally, the absorption and accumulation of other metal ions were enhanced, likely as a compensatory mechanism for iron scarcity. At the transcriptional level, iron deficiency induced the expression of genes involved in metal absorption and transport, as well as those related to photosynthesis, carbon metabolism, and ribosomal function, thereby supporting iron homeostasis and maintaining metabolic balance under stress conditions.<\/jats:p>","DOI":"10.3390\/plants14182934","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T10:05:17Z","timestamp":1758535517000},"page":"2934","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Physiological, Biochemical, and Transcriptomic Responses to Iron Deficiency in Two Potato Varieties"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6464-8454","authenticated-orcid":false,"given":"Xiangying","family":"Ma","sequence":"first","affiliation":[{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5423-1296","authenticated-orcid":false,"given":"Shenglong","family":"Yang","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"He","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangji","family":"Ye","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Aung, M.S., and Masuda, H. (2020). Corrigendum: How Does Rice Defend against Excess Iron?: Physiological and Molecular Mechanisms. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.601527"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev-arplant-042811-105522","article-title":"Iron Uptake, Translocation, and Regulation in Higher Plants","volume":"63","author":"Kobayashi","year":"2012","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1093\/jxb\/erz050","article-title":"Iron-Sulfur Protein NFU2 Is Required for Branched-Chain Amino Acid Synthesis in Arabidopsis Roots","volume":"70","author":"Touraine","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1093\/jxb\/erab531","article-title":"Plant Iron Nutrition: The Long Road from Soil to Seeds","volume":"73","author":"Murgia","year":"2022","journal-title":"J. Exp. Bot."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Li, W.F., and Lan, P. (2017). The Understanding of the Plant Iron Deficiency Responses in Strategy I Plants and the Role of Ethylene in This Process by Omic Approaches. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.00040"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1104\/pp.104.3.815","article-title":"Iron: Nutritious, Noxious, and Not Readily Available","volume":"104","author":"Guerinot","year":"1994","journal-title":"Plant Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1104\/pp.15.01194","article-title":"The Vacuolar Manganese Transporter MTP8 Determines Tolerance to Iron Deficiency-Induced Chlorosis in Arabidopsis","volume":"170","author":"Eroglu","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.plaphy.2011.01.026","article-title":"Towards a Knowledge-Based Correction of Iron Chlorosis","volume":"49","year":"2011","journal-title":"Plant Physiol. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s10533-007-9083-5","article-title":"Photosynthetic Responses in Phaeocystis antarctica towards Varying Light and Iron Conditions","volume":"83","author":"Stefels","year":"2007","journal-title":"Biogeochemistry"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.jprot.2009.01.024","article-title":"Hydroponics on a Chip: Analysis of the Fe Deficient Arabidopsis Thylakoid Membrane Proteome","volume":"72","author":"Laganowsky","year":"2009","journal-title":"J. Proteom."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1080\/01904168609363475","article-title":"Different Strategies in Higher Plants in Mobilization and Uptake of Iron","volume":"9","author":"Marschner","year":"1986","journal-title":"J. Plant Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/mp\/ssr065","article-title":"Fitting into the Harsh Reality: Regulation of Iron-Deficiency Responses in Dicotyledonous Plants","volume":"5","author":"Ivanov","year":"2012","journal-title":"Mol. Plant"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.plaphy.2012.08.003","article-title":"Iron Uptake and Homeostasis Related Genes in Potato Cultivated in Vitro under Iron Deficiency and Overload","volume":"60","author":"Legay","year":"2012","journal-title":"Plant Physiol. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chan-Rodriguez, D., and Walker, E.L. (2018). Analysis of Yellow Striped Mutants of Zea mays Reveals Novel Loci Contributing to Iron Deficiency Chlorosis. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.00157"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Expert, D., and O\u2019Brian, M.R. (2012). Molecular Aspects of Iron Metabolism in Pathogenic and Symbiotic Plant-Microbe Associations, Springer. SpringerBriefs in Molecular Science.","DOI":"10.1007\/978-94-007-5267-2"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1046\/j.1365-313X.2002.01381.x","article-title":"The Metal Ion Transporter IRT1 Is Necessary for Iron Homeostasis and Efficient Photosynthesis in Arabidopsis thaliana","volume":"31","author":"Varotto","year":"2002","journal-title":"Plant J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1105\/tpc.001388","article-title":"IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth","volume":"14","author":"Vert","year":"2002","journal-title":"Plant Cell"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Mai, H.J., Pateyron, S., and Bauer, P. (2016). Iron Homeostasis in Arabidopsis thaliana: Transcriptomic Analyses Reveal Novel FIT-Regulated Genes, Iron Deficiency Marker Genes and Functional Gene Networks. BMC Plant Biol., 16.","DOI":"10.1186\/s12870-016-0899-9"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gao, F., Robe, K., Gaymard, F., Izquierdo, E., and Dubos, C. (2019). The Transcriptional Control of Iron Homeostasis in Plants: A Tale of bHLH Transcription Factors?. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00006"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1104\/pp.104.054270","article-title":"Iron-Mediated Control of the Basic Helix-Loop-Helix Protein FER, a Regulator of Iron Uptake in Tomato","volume":"137","author":"Brumbarova","year":"2005","journal-title":"Plant Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3400","DOI":"10.1105\/tpc.104.024315","article-title":"The Essential Basic Helix-Loop-Helix Protein FIT1 Is Required for the Iron Deficiency Response","volume":"16","author":"Colangelo","year":"2004","journal-title":"Plant Cell"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1038\/sj.cr.7290331","article-title":"AtbHLH29 of Arabidopsis thaliana Is a Functional Ortholog of Tomato FER Involved in Controlling Iron Acquisition in Strategy I Plants","volume":"15","author":"Yuan","year":"2005","journal-title":"Cell Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Palmer, C.M., Hindt, M.N., Schmidt, H., Clemens, S., and Guerinot, M.L. (2013). MYB10 and MYB72 Are Required for Growth under Iron-Limiting Conditions. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003953"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.tplants.2014.07.005","article-title":"Iron Nutrition, Biomass Production, and Plant Product Quality","volume":"20","author":"Briat","year":"2015","journal-title":"Trends Plant Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Akyol, H., Riciputi, Y., Capanoglu, E., Caboni, M.F., and Verardo, V. (2016). Phenolic Compounds in the Potato and Its By-Products: An Overview. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17060835"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"474","DOI":"10.17221\/459\/2016-PSE","article-title":"Carotenoids in Potatoes\u2014A Short Overview","volume":"62","author":"Lachman","year":"2016","journal-title":"Plant Soil Environ."},{"key":"ref_27","unstructured":"Vreugdenhil, D. (2007). Potato Biology and Biotechnology: Advances and Perspectives, Elsevier B.V."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.scienta.2006.01.004","article-title":"Impact of Iron Stress on Biomass, Yield, Metabolism and Quality of Potato (Solanum tuberosum L.)","volume":"108","author":"Chatterjee","year":"2006","journal-title":"Sci. Hortic."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"589","DOI":"10.21273\/JASHS.134.6.589","article-title":"Relationship between the Hormonal Balance and the Regulation of Iron Deficiency Stress Responses in Cucumber","volume":"134","author":"Bacaicoa","year":"2009","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1021\/jf062740i","article-title":"Andean Potato Cultivars (Solanum tuberosum L.) as a Source of Antioxidant and Mineral Micronutrients","volume":"55","author":"Andre","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1002\/jsfa.2765","article-title":"Iron and Zinc Concentration of Native Andean Potato Cultivars from a Human Nutrition Perspective","volume":"87","author":"Burgos","year":"2007","journal-title":"J. Sci. Food Agric."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/nmeth.1226","article-title":"Mapping and Quantifying Mammalian Transcriptomes by RNA-Seq","volume":"5","author":"Mortazavi","year":"2008","journal-title":"Nat. Methods"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Young, M.D., Wakefield, M.J., Smyth, G.K., and Oshlack, A. (2010). Gene Ontology Analysis for RNA-seq: Accounting for Selection Bias. Genome Biol., 11.","DOI":"10.1186\/gb-2010-11-2-r14"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3787","DOI":"10.1093\/bioinformatics\/bti430","article-title":"Automated Genome Annotation and Pathway Identification Using the KEGG Orthology (KO) as a Controlled Vocabulary","volume":"21","author":"Mao","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Love, M.I., Huber, W., and Anders, S. (2014). Moderated Estimation of Fold Change and Dispersion for RNA-seq Data with DESeq2. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0550-8"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"D480","DOI":"10.1093\/nar\/gkm882","article-title":"KEGG for Linking Genomes to Life and the Environment","volume":"36","author":"Kanehisa","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s11104-007-9484-1","article-title":"Effect of Peanut Mixed Cropping with Gramineous Species on Micronutrient Concentrations and Iron Chlorosis of Peanut Plants Grown in a Calcareous Soil","volume":"306","author":"Zuo","year":"2008","journal-title":"Plant Soil"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1081\/PLN-120014711","article-title":"Effect of Iron Deficiency Stress on Iron-Reductase Activity at Plasmalemma of Apple Root Cells","volume":"25","author":"Han","year":"2002","journal-title":"J. Plant Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13007-019-0478-9","article-title":"Soybean Iron Deficiency Chlorosis High Throughput Phenotyping Using an Unmanned Aircraft System","volume":"15","author":"Dobbels","year":"2019","journal-title":"Plant Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1007\/s11099-017-0696-1","article-title":"Effect of Iron, Zinc and Manganese Shortage-Induced Change on Photosynthetic Pigments, Some Osmoregulators and Chlorophyll Fluorescence Parameters in Lettuce","volume":"56","author":"Roosta","year":"2018","journal-title":"Photosynthetica"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1023\/A:1004451728237","article-title":"Photosystem II Efficiency in Low Chlorophyll, Iron-Deficient Leaves","volume":"215","author":"Abadia","year":"1999","journal-title":"Plant Soil"},{"key":"ref_43","first-page":"339","article-title":"Technique of the Modulated Chlorophyll Fluorescence: Basic Concepts, Useful Parameters, and Some Applications","volume":"37","year":"1999","journal-title":"Photosynthetica"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"511","DOI":"10.17221\/2191-PSE","article-title":"Photosystem II of Barley Seedlings under Cadmium and Lead Stress","volume":"53","author":"Kalaji","year":"2007","journal-title":"Plant Soil Environ."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s11120-015-0093-1","article-title":"Low PSI Content Limits the Photoprotection of PSI and PSII in Early Growth Stages of Chlorophyll b-Deficient Wheat Mutant Lines","volume":"125","author":"Brestic","year":"2015","journal-title":"Photosynth. Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Barker, A.V., and Pilbeam, D.J. (2015). Handbook of Plant Nutrition, CRC Press. [2nd ed.].","DOI":"10.1201\/b18458"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1104\/pp.97.3.886","article-title":"Chlorophyll Fluorescence and Photon Yield of Oxygen Evolution in Iron-Deficient Sugar Beet (Beta vulgaris L.) Leaves","volume":"97","author":"Morales","year":"1991","journal-title":"Plant Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Wang, Y., Kang, Y., Zhong, M., Zhang, L., Liu, L., Zhang, Y., Lin, X., and Huang, H. (2022). Effects of Iron Deficiency Stress on Plant Growth and Quality in Flowering Chinese Cabbage and Its Adaptive Response. Agronomy, 12.","DOI":"10.3390\/agronomy12040875"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s13007-022-00855-5","article-title":"Development of a Controlled-Environment Assay to Induce Iron Deficiency Chlorosis in Soybean by Adjusting Calcium Carbonates, pH, and Nodulation","volume":"18","author":"Merry","year":"2022","journal-title":"Plant Methods"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1104\/pp.116.1.173","article-title":"Oxidative Damage in Pea Plants Exposed to Water Deficit or Paraquat","volume":"116","author":"Escuredo","year":"1998","journal-title":"Plant Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s10535-006-0011-7","article-title":"Antioxidant Defense Mechanism under Salt Stress in Wheat Seedlings","volume":"50","author":"Mandhania","year":"2006","journal-title":"Biol. Plant."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1134\/S1021443719020031","article-title":"Effect of Soil Drought on Biomass Production, Physiological Attributes and Antioxidant Enzymes Activities of Potato Cultivars","volume":"66","author":"Alhoshan","year":"2019","journal-title":"Russ. J. Plant Physiol."},{"key":"ref_53","first-page":"103","article-title":"Effects of Water Stress on Physiological Characteristics and Endogenous Hormone IAA and ABA Contents in Seedling Root of Potato","volume":"50","author":"Wang","year":"2018","journal-title":"J. Northeast Norm. Univ. Nat. Sci. Ed."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s00709-015-0800-y","article-title":"Physiological Performance and Differential Expression Profiling of Genes Associated with Drought Tolerance in Root Tissue of Four Contrasting Varieties of Two Gossypium Species","volume":"253","author":"Singh","year":"2016","journal-title":"Protoplasma"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Bi, Z., Dekomah, S.D., Wang, Y., Wang, L., Wang, Z., Wang, Y., Wang, H., Wang, X., Qin, Y., and Xu, J. (2024). Overexpression of StCDPK13 in Potato Enhances Tolerance to Drought Stress. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252312620"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s11104-019-04313-5","article-title":"Comparative Transcriptome Analysis Reveals Key Genes Responsible for the Homeostasis of Iron and Other Divalent Metals in Peanut Roots under Iron Deficiency","volume":"445","author":"Cao","year":"2019","journal-title":"Plant Soil"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1104\/pp.116.3.1063","article-title":"The Role of Iron-Deficiency Stress Responses in Stimulating Heavy-Metal Transport in Plants","volume":"116","author":"Cohen","year":"1998","journal-title":"Plant Physiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1007\/s11104-013-1861-3","article-title":"Effects of High Levels of Zinc and Manganese Ions on Strategy I Responses to Iron Deficiency in Citrus","volume":"373","author":"Iglesias","year":"2013","journal-title":"Plant Soil"},{"key":"ref_59","first-page":"1659","article-title":"Some of Micronutrients Homeostasis in Soybean","volume":"1","author":"Kobraee","year":"2011","journal-title":"J. Basic Appl. Sci. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1469-8137.2010.03606.x","article-title":"Differential Expression and Regulation of Iron-Regulated Metal Transporters in Arabidopsis halleri and Arabidopsis thaliana\u2014The Role in Zinc Tolerance","volume":"190","author":"Shanmugam","year":"2011","journal-title":"New Phytol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1093\/pcp\/pcr089","article-title":"AtIRT1, the Primary Iron Uptake Transporter in the Root, Mediates Excess Nickel Accumulation in Arabidopsis thaliana","volume":"52","author":"Nishida","year":"2011","journal-title":"Plant Cell Physiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"329","DOI":"10.4161\/psb.19263","article-title":"Induction of IRT1 by the Nickel-Induced Iron-Deficient Response in Arabidopsis","volume":"7","author":"Nishida","year":"2012","journal-title":"Plant Signal. Behav."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/17800","article-title":"A Ferric-Chelate Reductase for Iron Uptake from Soils","volume":"397","author":"Robinson","year":"1999","journal-title":"Nature"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1007\/s11738-013-1474-8","article-title":"Cloning and Characterization of MxHA7, a Plasma Membrane H+-ATPase Gene Related to High Tolerance of Malus xiaojinensis to Iron Deficiency","volume":"36","author":"Zha","year":"2014","journal-title":"Acta Physiol. Plant."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Ning, X., Lin, M., Huang, G., Mao, J., Gao, Z., and Wang, X. (2023). Research Progress on Iron Absorption, Transport, and Molecular Regulation Strategy in Plants. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1190768"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"5624","DOI":"10.1073\/pnas.93.11.5624","article-title":"A Novel Iron-Regulated Metal Transporter from Plants Identified by Functional Expression in Yeast","volume":"93","author":"Eide","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/18\/2934\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:46:41Z","timestamp":1760035601000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/14\/18\/2934"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,21]]},"references-count":66,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["plants14182934"],"URL":"https:\/\/doi.org\/10.3390\/plants14182934","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,21]]}}}