{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:59:26Z","timestamp":1780466366725,"version":"3.54.1"},"reference-count":47,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANID FONDECYT Postdoctorado","award":["3180267"],"award-info":[{"award-number":["3180267"]}]},{"DOI":"10.13039\/501100008736","name":"FONDEF","doi-asserted-by":"crossref","award":["ID17AI100007"],"award-info":[{"award-number":["ID17AI100007"]}],"id":[{"id":"10.13039\/501100008736","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008736","name":"FONDEF","doi-asserted-by":"crossref","award":["ID17I10094"],"award-info":[{"award-number":["ID17I10094"]}],"id":[{"id":"10.13039\/501100008736","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The search for sustainable alternatives to synthetic agrochemicals has fueled a growing interest in plant-derived bioactive compounds. Drimys winteri (canelo), a native Chilean tree of significant ethnobotanical importance, is a promising source of antifungal sesquiterpenes, such as polygodial and drimenol. This study describes the development of an in vitro clonal micropropagation platform for D. winteri that enables the production of plant material under controlled laboratory conditions, which is subsequently submitted to extraction to obtain these bioactive compounds. Four tailored culture media have been formulated for successful propagation, rooting of plantlets, and callus induction. Histological analysis confirmed the presence of meristemoids in the dedifferentiated calli. Furthermore, HPLC and GC-MS analyses indicate that phytochemical composition of extracts of in vitro-propagated D. winteri and those from mature, wild-grown trees is quite similar. This result is in line with the antifungal activity against Botrytis cinerea exhibited by these extracts; namely, both are comparable. This biotechnological approach offers a scalable method for producing plant-based antifungal agents, contributing to sustainable agriculture and the valorization of native genetic resources.<\/jats:p>","DOI":"10.3390\/plants14203215","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:23:34Z","timestamp":1760952214000},"page":"3215","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessment of Phytochemical Composition and Antifungal Activity of Micropropagated Drymis winteri Plants"],"prefix":"10.3390","volume":"14","author":[{"given":"Julia","family":"Rubio","sequence":"first","affiliation":[{"name":"Instituto Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Chile, Av. del Valle Sur 534, Santiago 8580640, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Robles-Kelly","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Universidad Aut\u00f3noma de Chile, El Llano, Santiago 8910339, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8745-7916","authenticated-orcid":false,"given":"Evelyn","family":"Silva-Moreno","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n e Innovaci\u00f3n en C\u00e1ncer, Fundaci\u00f3n Arturo L\u00f3pez P\u00e9rez, Jose Manuel Infante 805, Santiago 7500921, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2594-1697","authenticated-orcid":false,"given":"H\u00e9ctor","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Grupo QBAB, Instituto de Ciencias Aplicadas, Facultad de Ingenier\u00eda, Universidad Aut\u00f3noma de Chile, Av. del Valle Sur 534, Santiago 8580640, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8007-6264","authenticated-orcid":false,"given":"Andr\u00e9s F.","family":"Olea","sequence":"additional","affiliation":[{"name":"Grupo QBAB, Instituto de Ciencias Aplicadas, Facultad de Ingenier\u00eda, Universidad Aut\u00f3noma de Chile, Av. del Valle Sur 534, Santiago 8580640, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3670","DOI":"10.1016\/j.bbagen.2013.02.008","article-title":"Natural products: A continuing source of novel drug leads","volume":"1830","author":"Cragg","year":"2013","journal-title":"Biochim. 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