{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T14:07:18Z","timestamp":1774447638035,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the state assignments of the Ministry of Science and Higher Education of the Russian Federation","award":["122042600086-7"],"award-info":[{"award-number":["122042600086-7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Pothos (Epipremnum aureum G.S. Bunting), which belongs to the Arum family (Araceae Juss.), can be used for medicinal, ornamental, and pollutant-purifying purposes. Due to the usefulness of pothos, the market demand for this species is increasing. Our study attempts to fill in the shortcomings of previous studies on the effect of activated carbon and plant growth regulators on the ability of shoots to take root in vitro, as well as the effect of inexpensive and readily available materials on the transition of seedlings from in vitro to the greenhouse stage. To evaluate the shooting results, Murashige and Skoog medium (MS) was used, which included 6-benzylaminopurine (BA), kinetin (Kn), \u03b1-naphthaleneacetic acid (\u03b1-NAA), coconut water, activated carbon, and indole-3-butyric acid (IBA) in various concentrations and combinations. Our results showed that the MS medium with the addition of 2.5 mg\/L BA and 1.0 mg\/L Kn was optimal for propagation by shoots. In this variant, 2.86 shoots per explant, 1.87 cm of shoot length, and 1.59 leaves per shoot were obtained. Despite the fact that this treatment provided the highest total cytokinin concentration, it was significantly more effective than only BA (2.5 mg\/L) and all combinations of BA+\u03b1-NAA or Kn+\u03b1-NAA. For rooting, the micro shoots obtained on the above medium were transferred to MS + 0.25 mg\/L \u03b1-NAA + 0.5 g\/L AC, which allowed for rooting by 93.33%, 1.93 roots per explant, and root lengths by 2.37 cm. This is higher than with the IBA-based treatment, which led to a shortening of the roots and a reduction in their branching. Acclimatization in a 1:1 mixture (by volume) of loamy garden soil (pH 6.2, 2.1% organic matter) and coconut coir (particle size 0.5\u20132 mm) gave 75% survival after 40 days. These results have opened up the prospect of developing an effective method for reproducing pothos species in vitro by organogenesis at the lowest cost.<\/jats:p>","DOI":"10.3390\/plants14213299","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:50:19Z","timestamp":1761785419000},"page":"3299","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Direct Organogenesis of Epipremnum aureum G.S. Bunting for Mass Propagation"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0211-1196","authenticated-orcid":false,"given":"Hai T.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Plant Biotechnology, Vietnam National University of Agriculture, Gia Lam, Hanoi 12406, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quyet V.","family":"Khuat","sequence":"additional","affiliation":[{"name":"Department of Biology and Agricultural Engineering, Hanoi Pedagogical University N\u00b02, Nguyen Van Linh, 32, Phuc Yen  15000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thao T.","family":"Ninh","sequence":"additional","affiliation":[{"name":"Department of Plant Biotechnology, Vietnam National University of Agriculture, Gia Lam, Hanoi 12406, Vietnam"},{"name":"Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 306 Carmody Rd., St. Lucia, QLD 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8877-4187","authenticated-orcid":false,"given":"Anh T. P.","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Plant Biotechnology, Vietnam National University of Agriculture, Gia Lam, Hanoi 12406, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le T.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Plant Biotechnology, Vietnam National University of Agriculture, Gia Lam, Hanoi 12406, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-1789","authenticated-orcid":false,"given":"Elena A.","family":"Kalasn\u0131kova","sequence":"additional","affiliation":[{"name":"Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya, 35, 127276 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulmalik A.","family":"Batukaev","sequence":"additional","affiliation":[{"name":"Chechen Research Institute of Agriculture, Lilovaya St., 1, 366021 Grozny, Russia"},{"name":"Department of Fruit and Vegetable Growing and Viticulture, Chechen State University, A. Sheripova, 32, 364061 Grozny, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5244-4311","authenticated-orcid":false,"given":"Rima N.","family":"Kirakosyan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Russian State Agrarian University\u2014Moscow Timiryazev Agricultural Academy, Timiryazevskaya, 49, 127550 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"ref_1","unstructured":"Croat, T.B. (1979). The Distribution of Araceae, Tropical Botany, Academic Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.sajb.2016.12.005","article-title":"Assessing and managing the threat posed by Epipremnum aureum in South Africa","volume":"109","author":"Moodley","year":"2017","journal-title":"S. Afr. J. 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