{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:19:15Z","timestamp":1790914755987,"version":"4.1.0"},"reference-count":69,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T00:00:00Z","timestamp":1765584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002641","name":"Konkuk University","doi-asserted-by":"crossref","award":["2025-A019-0203"],"award-info":[{"award-number":["2025-A019-0203"]}],"id":[{"id":"10.13039\/501100002641","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The present study investigated the effects of copper oxide nanoparticles (CuO NPs) at concentrations of 0, 5, 10, 20, and 40 mg\/L on micropropagation and the accumulation of lipophilic metabolites in Ajuga multiflora, a medicinally valuable ornamental species. The highest number of adventitious shoots (29.4 shoots per explant) was obtained on the shoot induction medium with 5 mg\/L CuO NPs. Shoot production gradually decreased at higher CuO NPs concentrations, falling to just 1.1 shoots per explant at 40 mg\/L CuO NPs. A similar pattern was seen in axillary shoot multiplication (22.4 shoots per explant at 5 mg\/L CuO NPs). However, the maximum shoot fresh weight (0.269 g) was reached on the shoot multiplication medium containing 10 mg\/L CuO NPs. Root induction was most effective at 5\u201310 mg\/L CuO NPs, while higher concentrations (20 or 40 mg\/L CuO NPs) suppressed or inhibited root formation and altered plantlet morphology. Notably, this study is among the first to assess CuO NPs\u2019 effects across multiple regeneration stages rather than focusing on just one morphogenic event. This emphasizes the importance of optimizing the dose not only for initial shoot induction but also for later multiplication and rooting, ensuring effective micropropagation. Metabolite analysis showed that both the type of organ (microshoots vs. leaves) and CuO NPs concentration significantly affected the levels of \u03b1-tocopherol, carotenoids, sterols, and fatty acids. Leaves had higher amounts of \u03b1-tocopherol and total carotenoids compared to microshoots. The phytosterol levels also varied, with leaves containing more 22-dehydroclerosterol and total phytosterols, while microshoots had more clerosterol. Treatment with 5 mg\/L CuO NPs increased phytosterol accumulation in both organs. CuO NPs significantly influenced the fatty acid profiles. In microshoots, total polyunsaturated fatty acids (PUFAs) increased and total saturated fatty acids (SFAs) decreased with higher CuO NPs levels. Conversely, in leaves, higher CuO NPs concentrations led to increased SFAs and decreased PUFAs, along with a significant rise in the omega-6 (n-6)\/n-3 PUFAs ratio. These findings suggest that controlled application of CuO NPs can serve as an elicitor to boost phytochemical production during micropropagation.<\/jats:p>","DOI":"10.3390\/plants14243807","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:32:37Z","timestamp":1765805557000},"page":"3807","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Copper Oxide Nanoparticles on Adventitious Shoot Regeneration, Axillary Shoot Multiplication, Rooting, and Bioactive Compounds in Ajuga multiflora Bunge"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7447-6392","authenticated-orcid":false,"given":"Iyyakkannu","family":"Sivanesan","sequence":"first","affiliation":[{"name":"Department of Environmental Health Science, Human and Eco Care Center, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5152-6761","authenticated-orcid":false,"given":"Shuchi","family":"Upadhyay","sequence":"additional","affiliation":[{"name":"School of Health Sciences and Technology, UPES, Dehradun 248007, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Soo","family":"Keum","sequence":"additional","affiliation":[{"name":"Department of Crop Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0845-9783","authenticated-orcid":false,"given":"Se Chul","family":"Chun","sequence":"additional","affiliation":[{"name":"Department of Environmental Health Science, Human and Eco Care Center, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9052-9941","authenticated-orcid":false,"given":"Ramesh Kumar","family":"Saini","sequence":"additional","affiliation":[{"name":"School of Health Sciences and Technology, UPES, Dehradun 248007, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lakhani, K.G., Hamid, R., Motamedi, E., and Marviya, G.V. (2025). A review on plant metabolite-mediated nanoparticle synthesis: Sustainable applications in horticultural crops. Front. Nanotechnol., 7.","DOI":"10.3389\/fnano.2025.1545413"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s11627-025-10539-z","article-title":"Mini-review: Application of nanoparticles in plant biotechnology and agriculture","volume":"61","author":"Andujar","year":"2025","journal-title":"Vitr. Cell. Dev. Biol.-Plant"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kang, I., and Sivanesan, I. (2025). Micropropagation of Philodendron \u2018White Knight\u2019 via Shoot Regeneration from Petiole Explants. Plants, 14.","DOI":"10.3390\/plants14111714"},{"key":"ref_4","first-page":"63","article-title":"Micropropagation of Ardisia mamillata Hance through axillary shoot multiplication","volume":"24","author":"Chun","year":"2024","journal-title":"Propag. Ornam. Plants"},{"key":"ref_5","first-page":"31","article-title":"Micropropagation of Monstera deliciosa Liebm.\u2018Thai Constellation\u2019","volume":"23","author":"Sivanesan","year":"2023","journal-title":"Propag. Ornam. Plants"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Soni, S., Singh, K.M., Jha, A.B., Dubey, R.S., and Sharma, P. (2026). Size-based dynamics of nanoparticles in plant growth and environmental stress tolerance: Potential benefits and hazards. Environ. Sci. Nano.","DOI":"10.1039\/D5EN00785B"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"46393","DOI":"10.1021\/acsomega.5c00987","article-title":"Foliar Application of Calcium Phosphate Nanoparticles Improves Photosynthetic Efficiency, Nutrient Uptake, and Antioxidant Capacity in Coriandrum sativum L. under Salinity Stress","volume":"10","author":"Kaleem","year":"2025","journal-title":"ACS Omega"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Djanaguiraman, M., Anbazhagan, V., Dhankher, O.P., and Prasad, P.V.V. (2024). Uptake, Translocation, Toxicity, and Impact of Nanoparticles on Plant Physiological Processes. Plants, 13.","DOI":"10.3390\/plants13223137"},{"key":"ref_9","unstructured":"Tombuloglu, H., Tombuloglu, G., Al-Suhaimi, E., Baykal, A., and Hakeem, K.R. (2024). Nanoparticles: Unveiling the impact on biochemical reactions in plants. Molecular Impacts of Nanoparticles on Plants and Algae, Academic Press."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s11240-024-02833-2","article-title":"Role of metal nanoparticles in organogenesis, secondary metabolite production and genetic transformation of plants under in vitro condition: A comprehensive review","volume":"158","author":"Prasad","year":"2024","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Al-Khayri, J.M., Rashmi, R., Surya Ulhas, R., Sudheer, W.N., Banadka, A., Nagella, P., Aldaej, M.I., Rezk, A.A.-S., Shehata, W.F., and Almaghasla, M.I. (2023). The Role of Nanoparticles in Response of Plants to Abiotic Stress at Physiological, Biochemical, and Molecular Levels. Plants, 12.","DOI":"10.3390\/plants12020292"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1049\/nbt2.12005","article-title":"Nanoparticles as elicitors and harvesters of economically important secondary metabolites in higher plants: A review","volume":"15","author":"Lala","year":"2021","journal-title":"IET Nanobiotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"120097","DOI":"10.1016\/j.indcrop.2024.120097","article-title":"Nanoparticles as elicitors and stimulators for plant tissue culture, transgenics, and genome editing: A comprehensive review","volume":"222","author":"Rohela","year":"2024","journal-title":"Ind. Crops Prod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"102994","DOI":"10.1016\/j.bcab.2023.102994","article-title":"Green fabrication of copper nano-fertilizer for enhanced crop yield in cowpea cultivar: A sustainable approach","volume":"56","author":"Mustafa","year":"2024","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1002\/ps.7857","article-title":"Copper oxide nanoparticles: An effective suppression tool against bacterial leaf blight of rice and its impacts on plants","volume":"80","author":"Ogunyemi","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6000","DOI":"10.1038\/s41598-024-56709-8","article-title":"The effects of green and chemically-synthesized copper oxide nanoparticles on the production and gene expression of morphinan alkaloids in Oriental poppy","volume":"14","author":"Khaldari","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s00299-024-03297-6","article-title":"Micronutrient deficiency-induced oxidative stress in plants","volume":"43","author":"Gupta","year":"2024","journal-title":"Plant Cell Rep."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Xu, E., Liu, Y., Gu, D., Zhan, X., Li, J., Zhou, K., Zhang, P., and Zou, Y. (2024). Molecular Mechanisms of Plant Responses to Copper: From Deficiency to Excess. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25136993"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s11240-023-02541-3","article-title":"Casein stabilized metal and metal oxide nanoparticle to eradicate the endophytic bacterial contamination from in vitro culturing of Scoparia dulcis L.","volume":"155","author":"Rakhimol","year":"2023","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1590\/1519-6984.232937","article-title":"Induction of secondary metabolites on nanoparticles stress in callus culture of Artemisia annua L.","volume":"81","author":"Fatima","year":"2021","journal-title":"Braz. J. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1080\/21691401.2021.1984935","article-title":"Copper oxide (CuO) and manganese oxide (MnO) nanoparticles induced biomass accumulation, antioxidants biosynthesis and abiotic elicitation of bioactive compounds in callus cultures of Ocimum basilicum (Thai basil)","volume":"49","author":"Nazir","year":"2021","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s11240-018-1512-8","article-title":"Effects of ZnO, CuO and \u03b3-Fe3O4 nanoparticles on mature embryo culture of wheat (Triticum aestivum L.)","volume":"136","author":"Nalci","year":"2018","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1080\/21645698.2021.1917975","article-title":"Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticum aestivum L.)","volume":"12","author":"Malik","year":"2021","journal-title":"GM Crops Food"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1007\/s11240-019-01588-5","article-title":"Effects of copper oxide nanoparticles to Solanum nigrum and its potential for phytoremediation","volume":"137","author":"Othman","year":"2019","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s12355-017-0539-1","article-title":"Elicitation of Secondary Metabolites in Callus Cultures of Stevia rebaudiana Bertoni Grown Under ZnO and CuO Nanoparticles Stress","volume":"20","author":"Javed","year":"2017","journal-title":"Sugar Tech."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"113228","DOI":"10.1016\/j.scienta.2024.113228","article-title":"Exploring somatic embryogenesis in Ajuga multiflora Bunge: Profiling lipophilic metabolites via HPLC, GC-FID, and GCMS\u2013 analysis","volume":"332","author":"Park","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.scienta.2018.03.056","article-title":"Impact of light quality and sucrose on adventitious shoot regeneration and bioactive compound accumulation in Ajuga multiflora Bunge","volume":"236","author":"Jeong","year":"2018","journal-title":"Sci. Hortic."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF02871693","article-title":"Pesticidal character of phytoecdysteroids from Ajuga multiflora Bunge (Labiatae) on larvae of Cryptorrhynchus lapathi L. (Coleoptera: Curculionidae)","volume":"13","year":"2002","journal-title":"J. For. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1080\/14786419.2022.2115491","article-title":"Chemical constituents of the Ajuga multiflora bunge and their protective effects on dexamethasone-induced muscle atrophy in C2C12 myotubes","volume":"37","author":"Lee","year":"2023","journal-title":"Nat. Prod. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Popova, E., Titova, M., Tynykulov, M., Zakirova, R.P., Kulichenko, I., Prudnikova, O., and Nosov, A. (2023). Sustainable Production of Ajuga Bioactive Metabolites Using Cell Culture Technologies: A Review. Nutrients, 15.","DOI":"10.3390\/nu15051246"},{"key":"ref_31","first-page":"72","article-title":"Studies on the cytotoxicity and antimicrobial effects of the extract of Ajuga multiflora Bunge","volume":"31","year":"2000","journal-title":"Korean J. Pharmacogn."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s13205-016-0376-z","article-title":"In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge","volume":"6","author":"Sivanesan","year":"2016","journal-title":"3 Biotech"},{"key":"ref_33","first-page":"75","article-title":"Studies on the Constituents of the Herbs of Ajuga multiflora (I)","volume":"29","author":"Yu","year":"1998","journal-title":"Korean J. Pharmacogn."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1007\/s10529-017-2376-4","article-title":"Micropropagation of Ajuga species: A mini review","volume":"39","author":"Park","year":"2017","journal-title":"Biotechnol. Lett."},{"key":"ref_35","first-page":"156","article-title":"Influence of cytokinins on adventitious shoot regeneration from leaf and petiole explants of Ajuga multiflora bunge","volume":"11","author":"Sivanesan","year":"2011","journal-title":"Propag. Ornam. Plants"},{"key":"ref_36","first-page":"42","article-title":"Effect of plant growth regulators on axillary shoot multiplication from nodal explants of Ajuga multiflora Bunge","volume":"15","author":"Park","year":"2015","journal-title":"Propag. Ornam. Plants"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/BF03030416","article-title":"Genetic transformation of Ajuga multiflora bunge with Agrobacterium rhizogenes and 20-hydroxyecdysone production in hairy roots","volume":"48","author":"Kim","year":"2005","journal-title":"J. Plant Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s13205-018-1117-2","article-title":"beta-Ecdysterone accumulation and regulation in Ajuga multiflora Bunge suspension culture","volume":"8","author":"Wang","year":"2018","journal-title":"3 Biotech"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.jphotobiol.2018.04.044","article-title":"Impacts of hormonal elicitors and photoperiod regimes on elicitation of bioactive secondary volatiles in cell cultures of Ajuga bracteosa","volume":"183","author":"Ali","year":"2018","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"47945","DOI":"10.1039\/C7RA06399G","article-title":"Effect of pRi T-DNA genes and elicitation on morphology and phytoecdysteroid biosynthesis in Ajuga bracteosa hairy roots","volume":"7","author":"Kayani","year":"2017","journal-title":"RSC Adv."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1186\/s13568-021-01297-3","article-title":"Differential induction of antioxidant and anti-inflammatory phytochemicals in agitated micro-shoot cultures of Ajuga integrifolia Buch. Ham. ex D.Don with biotic elicitors","volume":"11","author":"Ullah","year":"2021","journal-title":"AMB Express"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"112098","DOI":"10.1016\/j.indcrop.2020.112098","article-title":"Exogenous application of salicylic acid and gibberellic acid on biomass accumulation, antioxidant and anti-inflammatory secondary metabolites production in multiple shoot culture of Ajuga integrifolia Buch. Ham. ex D.Don","volume":"145","author":"Ullah","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"El-Naggar, H.M., and Osman, A.R. (2024). Enhancing growth and bioactive metabolites characteristics in Mentha pulegium L. via silicon nanoparticles during in vitro drought stress. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-05313-z"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, S., Mansinhos, I., Rodr\u00edguez-Solana, R., Pereira-Caro, G., Moreno-Rojas, J.M., and Romano, A. (2021). Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: Lavandula viridis L\u2019H\u00e9r and Thymus lotocephalus G. L\u00f3pez and R. Morales. Molecules, 26.","DOI":"10.3390\/molecules26216427"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s11240-021-02224-x","article-title":"Silicon dioxide and silver nanoparticles elicit antimicrobial secondary metabolites while enhancing growth and multiplication of Lavandula officinalis in-vitro plantlets","volume":"149","author":"Khattab","year":"2022","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"98020","DOI":"10.1007\/s11356-023-29384-x","article-title":"Titanium dioxide nanoparticles improve element uptake, antioxidant properties, and essential oil productivity of Melissa officinalis L. seedlings under in vitro drought stress","volume":"30","author":"Razavizadeh","year":"2023","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"37","DOI":"10.35206\/jan.1464551","article-title":"Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L.","volume":"8","year":"2025","journal-title":"J. Apitherapy Nat."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"100102","DOI":"10.1016\/j.plana.2024.100102","article-title":"Transforming plant tissue culture with nanoparticles: A review of current applications","volume":"10","author":"Gunasena","year":"2024","journal-title":"Plant Nano Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"36492","DOI":"10.1039\/C7RA07025J","article-title":"Nanomaterials in plant tissue culture: The disclosed and undisclosed","volume":"7","author":"Kim","year":"2017","journal-title":"RSC Adv."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3555","DOI":"10.1007\/s12668-024-01548-0","article-title":"Nano-gardening: Harnessing Metal Nanoparticles for Enhanced In Vitro Plant Regeneration","volume":"14","author":"Guha","year":"2024","journal-title":"BioNanoScience"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s12010-016-2217-2","article-title":"CuO Nanoparticles Inhibited Root Growth from Brassica nigra Seedlings but Induced Root from Stem and Leaf Explants","volume":"181","author":"Zafar","year":"2017","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Paramo, L.A., Feregrino-Perez, A.A., Guevara, R., Mendoza, S., and Esquivel, K. (2020). Nanoparticles in Agroindustry: Applications, Toxicity, Challenges, and Trends. Nanomaterials, 10.","DOI":"10.3390\/nano10091654"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s11051-024-06103-2","article-title":"A review: Improvement of plant tissue culture applications by using nanoparticles","volume":"26","author":"Balamurugan","year":"2024","journal-title":"J. Nanoparticle Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"012048","DOI":"10.1088\/1755-1315\/875\/1\/012048","article-title":"The impact of copper oxide and silver nanoparticles on woody plants obtained by in vitro method","volume":"875","author":"Fedorova","year":"2021","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Zakharova, O., Gusev, A., Strekalova, N., Grigoriev, G., Chebotareva, S., Baranchikov, P., and Kataranova, A. (2024). Copper oxide nanoparticles in clonal micropropagation of red oak. BIO Web of Conferences, EDP Sciences.","DOI":"10.1051\/bioconf\/202414502006"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.ecoenv.2014.12.013","article-title":"Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)","volume":"113","author":"Nair","year":"2015","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4434","DOI":"10.1021\/es204212z","article-title":"Xylem- and phloem-based transport of CuO nanoparticles in maize (Zea mays L.)","volume":"46","author":"Wang","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Pathak, A., Haq, S., Meena, N., Dwivedi, P., Kothari, S.L., and Kachhwaha, S. (2023). Multifaceted Role of Nanomaterials in Modulating In Vitro Seed Germination, Plant Morphogenesis, Metabolism and Genetic Engineering. Plants, 12.","DOI":"10.3390\/plants12173126"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"9548","DOI":"10.1021\/acs.est.3c00864","article-title":"Cu(I)\/Cu(II) Released by Cu Nanoparticles Revealed Differential Cellular Toxicity Related to Mitochondrial Dysfunction","volume":"57","author":"Wang","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Xiong, T., Zhang, S., Kang, Z., Zhang, T., and Li, S. (2021). Dose-Dependent Physiological and Transcriptomic Responses of Lettuce (Lactuca sativa L.) to Copper Oxide Nanoparticles\u2014Insights into the Phytotoxicity Mechanisms. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073688"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1590\/S1677-04202005000100012","article-title":"Copper in plants","volume":"17","author":"Yruela","year":"2005","journal-title":"Braz. J. Plant Physiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Iqbal, Z., Javad, S., Naz, S., Shah, A.A., Shah, A.N., Paray, B.A., Gulnaz, A., and Abdelsalam, N.R. (2022). Elicitation of the in vitro Cultures of Selected Varieties of Vigna radiata L. With Zinc Oxide and Copper Oxide Nanoparticles for Enhanced Phytochemicals Production. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.908532"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"5221","DOI":"10.1007\/s12010-025-05284-3","article-title":"Alhagi maurorum: A Medicinal Treasure Trove Empowered by Copper Oxide Nanoparticles for Enhanced Secondary Metabolite Synthesis","volume":"197","author":"Bamal","year":"2025","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures","volume":"15","author":"Murashige","year":"2006","journal-title":"Physiol. Plant."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.foodchem.2018.05.104","article-title":"An efficient one-step scheme for the purification of major xanthophyll carotenoids from lettuce, and assessment of their comparative anticancer potential","volume":"266","author":"Saini","year":"2018","journal-title":"Food Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.foodres.2017.11.028","article-title":"Metabolite profiling of green, green\/red, and red lettuce cultivars: Variation in health beneficial compounds and antioxidant potential","volume":"105","author":"Kim","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.foodchem.2017.07.099","article-title":"Carotenoid extraction methods: A review of recent developments","volume":"240","author":"Saini","year":"2018","journal-title":"Food Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1007\/s12161-012-9526-z","article-title":"Validation of a Single-Extraction Procedure for Sequential Analysis of Vitamin E, Cholesterol, Fatty Acids, and Total Fat in Seafood","volume":"6","author":"Cruz","year":"2012","journal-title":"Food Anal. Methods"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.sajb.2021.05.022","article-title":"Edible mushrooms show significant differences in sterols and fatty acid compositions","volume":"141","author":"Saini","year":"2021","journal-title":"S. Afr. J. 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