{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:50:47Z","timestamp":1782363047861,"version":"3.54.5"},"reference-count":52,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82004014"],"award-info":[{"award-number":["82004014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Chlorogenic acid (CGA) is an active ingredient widely found in plants and has been shown to have potential blood-glucose-lowering effects. However, the research on the efficient extraction processes of CGA from sweet potatoes and its systematic mechanisms underlying hypoglycemic effects is still insufficient. This study optimized the extraction of CGA from various sweet potato parts and varieties using ethanol, and predicted the hypoglycemic mechanism of sweet potato leaves via network pharmacology and molecular docking. The efficacy of the leaf extracts was demonstrated through the in vitro inhibition of hepatic glucose output, accompanied by minimal cytotoxicity, and was further validated in an acute mouse model. The results demonstrated that the optimal extraction conditions were an ethanol concentration of 65.48%, a liquid\u2013solid ratio of 39.00 mL\u00b7g\u22121, an ultrasonic time of 50.00 min, and a temperature of 45 \u00b0C. The final extraction yield of CGA crude extract was 3.54%, with the highest content in sweet potato leaves, suggesting a multi-target synergistic mechanism of action for sweet potato leaves. Further in vitro experiments indicated that the CGA crude extract can exert hypoglycemic effects by inhibiting hepatic gluconeogenesis. In conclusion, the study lays a foundation for the further purification and utilization of sweet potato CGA, and establishes a theoretical basis for the development of sweet potato leaf resources as hypoglycemic functional ingredients.<\/jats:p>","DOI":"10.3390\/plants15010120","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T09:18:56Z","timestamp":1767345536000},"page":"120","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Optimization of Sweet Potato (Ipomoea batatas L.) Chlorogenic Acid Extraction Process and Hypoglycemic Effect Study"],"prefix":"10.3390","volume":"15","author":[{"given":"Xiaofei","family":"Wang","sequence":"first","affiliation":[{"name":"College of Agronomy, Shandong Agriculture University, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shandong Agriculture University, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shandong Agriculture University, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohan","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shandong Agriculture University, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shandong Agriculture University, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingli","family":"Han","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shandong Agriculture University, Tai\u2019an 271018, China"},{"name":"National Key Laboratory for Tropical Crop Breeding & Haikou Key Laboratory for Research and Utilization of Tropical Natural Products, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shandong Agriculture University, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,1]]},"reference":[{"key":"ref_1","first-page":"7","article-title":"Exploring the integration and development path of sweet potato industry in yuzhou, Henan Province","volume":"8","author":"Wang","year":"2022","journal-title":"Rural. Agric. Farmers (B Ed.)"},{"key":"ref_2","first-page":"392","article-title":"Comparative study on starch composition, physicochemical properties and noodle quality of different sweet potato varieties","volume":"36","author":"Hou","year":"2022","journal-title":"Acta Agric. Nucl. Sin."},{"key":"ref_3","first-page":"30","article-title":"Biological activity of chlorogenic acid and progress of sweet potato chlorogenic acid research","volume":"35","author":"Wang","year":"2017","journal-title":"J. Jiangsu Norm. Univ. (Nat. Sci. Ed.)"},{"key":"ref_4","first-page":"205","article-title":"Research progress on nutritional functions and processing utilization of sweet potato","volume":"43","author":"Shi","year":"2022","journal-title":"Food Res. Dev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1039\/c3fo60194c","article-title":"The impact of in vitro digestion on bioaccessibility of polyphenols from potatoes and sweet potatoes and their influence on iron absorption by human intestinal cells","volume":"4","author":"Miranda","year":"2013","journal-title":"Food Funct."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"27861","DOI":"10.1021\/acs.jafc.4c05796","article-title":"Mechanistic study of purple sweet potato anthocyanins: Multifaceted anti-fibrotic effects and targeting of PDGFR\u03b2 in liver fibrosis","volume":"72","author":"Dai","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","unstructured":"NCD Risk Factor Collaboration (NCD\u2014RisC) (2016). Worldwide trends in diabetes since 1980: A pooled analysis of 751 population\u2014Based studies with 4.41million participants. Lancet, 387, 1513\u20131530."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/S0955-0674(99)00073-3","article-title":"Regulation of cAMP and cGMP signaling: New phospho diesterases and new functions","volume":"12","author":"Soderling","year":"2000","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_9","first-page":"1332","article-title":"Regulation of gluconeogenesis in liver, kidney and intestine by glucagon","volume":"39","author":"Su","year":"2023","journal-title":"Chin. Pharmac. Bulletin."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1016\/j.cmet.2022.10.001","article-title":"The past, present, and future physiology and pharmacology of glucagon","volume":"34","author":"Capozzi","year":"2022","journal-title":"Cell Metab."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"110147","DOI":"10.1016\/j.foodres.2021.110147","article-title":"Three phytosterols from sweet potato inhibit MCF7-xenograft-tumor growth through modulating gut microbiota homeostasis and SCFAs secretion","volume":"141","author":"Han","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Liu, C., Miao, Y., Zhou, W., Ma, Y., Guo, W., and Li, A. (2024). Impact of thermal processing on the structure, antioxidant properties and hypoglycemic activities of sweet potato polysaccharides. Foods, 13.","DOI":"10.3390\/foods13193082"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lee, C., Yoon, Y., Yoon, Y., Chung, K., Kim, M., Park, G., Choi, M., Jang, Y., and Lee, K. (2025). Protective effects of a standardized water extract from the stem of Ipomoea batatas L. against high-fat diet-induced obesity. Nutrient, 17.","DOI":"10.3390\/nu17101643"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11844","DOI":"10.3390\/app112411844","article-title":"Comparative study of potato (Solanum tuberosum L.) and sweet potato (Ipomoea batatas L.): Evaluation of proximate composition, polyphenol content, mineral and sntioxidant sctivities","volume":"11","author":"Ammara","year":"2021","journal-title":"Appl. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"144657","DOI":"10.1016\/j.foodchem.2025.144657","article-title":"Encapsulated purple sweet potato peel extract as antioxidant and sustainable colourant to preserve the quality of beef burgers during the shelf life","volume":"487","author":"Carrillo","year":"2025","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Bian, S., Hu, J., Liu, G., Peng, S., Chen, H., Jiang, Z., Wang, T., Ye, Q., and Zhu, H. (2022). Natural deep eutectic solvent-based microwave-assisted extraction of total flavonoid compounds from spent sweet potato (Ipomoea batatas L.) leaves: Optimization and antioxidant and bacteriostatic Activity. Molecules, 27.","DOI":"10.3390\/molecules27185985"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"10","DOI":"10.29219\/fnr.v64.3609","article-title":"White sweet potato ameliorates hyperglycemia and regenerates pancreatic islets in diabetic mice","volume":"64","author":"Shih","year":"2020","journal-title":"Food Nutr. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1021\/acs.jafc.6b04788","article-title":"Selenylation of polysaccharide from the sweet potato and evaluation of antioxidant, antitumor, and antidiabetic activities","volume":"65","author":"Yuan","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","unstructured":"Luo, D. (2020). Hypoglycemic Effect and Mechanism of Polyphenols from Stems and Leaves of Sweet Potato \u2018Ximeng 1\u2019. [Ph.D. Thesis, Chinese Academy of Agricultural Sciences]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e71069","DOI":"10.1002\/fsn3.71069","article-title":"Network pharmacology and molecular docking elucidate the mechanism of phillyrin in colorectal cancer","volume":"13","author":"Hu","year":"2025","journal-title":"Food Sci. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bae, J., Park, W., Kim, H., Kim, H., Kang, K., Kwak, S., and Ahn, M. (2021). Protective effect of carotenoid extract from orange-fleshed sweet potato on gastric ulcer in mice by inhibition of NO, IL-6 and PGE2 Production. Pharmaceuticals, 14.","DOI":"10.3390\/ph14121320"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"140133","DOI":"10.1016\/j.ijbiomac.2025.140133","article-title":"Chlorogenic acid mitigates heat stress-induced oxidative damage in bovine mammary epithelial cells by inhibiting NF-\u03baB-mediated NLRP3 inflammasome activation via upregulating the Nrf2 signaling pathway","volume":"301","author":"Huang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"109989","DOI":"10.1016\/j.intimp.2023.109989","article-title":"Isochlorogenic acid A alleviates dextran sulfate sodium-induced ulcerative colitis in mice through STAT3\/NF-\u03baB pathway","volume":"118","author":"Tang","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1002\/mnfr.201601118","article-title":"Structural constraints and importance of caffeic acid moiety for anti-hyperglycemic effects of caffeoylquinic acids from chicory","volume":"61","author":"Jackson","year":"2017","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4141","DOI":"10.1021\/jf9044827","article-title":"Contribution of chlorogenic acids to the inhibition of human hepatic glucose-6-phosphatase activity in vitro by Svetol, a standardized decaffeinated green coffee extract","volume":"58","author":"Ibarra","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Xiao, N., Zhang, T., Han, M., Tian, D., Liu, J., Li, S., Yang, L., and Pan, G. (2023). Chlorogenic acid inhibits ceramide accumulation to restrain hepatic glucagon response. Nutrients, 15.","DOI":"10.3390\/nu15143173"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jep.2010.04.016","article-title":"Gluconeogenesis inhibition and phytochemical composition of two Cecropia species","volume":"130","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5318245","DOI":"10.1155\/2022\/5318245","article-title":"Insulin-related liver pathways and the therapeutic effects of aerobic training, green coffee, and chlorogenic acid supplementation in prediabetic mice","volume":"2022","author":"Abdollahi","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_29","first-page":"54","article-title":"Optimization of extraction process and stability study of chlorogenic acid from dandelion","volume":"23","author":"Liu","year":"2023","journal-title":"China Feed"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Mangiapelo, L., Blasi, F., Ianni, F., Barola, C., Galarini, R., Abualzulof, G., Sardella, R., Volpi, C., and Cossignani, L. (2023). Optimization of ultrasound-assisted extraction of chlorogenic acid from potato sprout waste and enhancement of the in vitro total antioxidant capacity. Antioxidants, 12.","DOI":"10.3390\/antiox12020348"},{"key":"ref_31","first-page":"84","article-title":"Optimization of chlorogenic acid extraction from the leaves of Sambucus chinensis Lindl. by response surface methodology","volume":"7","author":"Chen","year":"2022","journal-title":"Hunan Agric. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"107579","DOI":"10.1016\/j.ultsonch.2025.107579","article-title":"Deep eutectic solvent extraction of chlorogenic acid from dandelion with ultrasonic-assisted: Process optimization, purification, and bioactivity","volume":"122","author":"Liu","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_33","first-page":"532","article-title":"Synchronization isolation method for multiple types of cells from mouse liver","volume":"31","author":"Gan","year":"2023","journal-title":"Zhonghua Gan Zang Bing Za Zhi"},{"key":"ref_34","unstructured":"Wang, Y. (2019). Extraction and Genotypic Varietion of Antioxidant Functional Components from Sweet Potato Vines. [Master\u2019s Thesis, Zhejiang A&F University]."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chiu, C., Liu, K., Lin, H., Chu, W., Lai, Y., and Chao, P. (2022). Analysis of Chlorogenic Acid in Sweet Potato Leaf Extracts. Plants, 11.","DOI":"10.3390\/plants11152063"},{"key":"ref_36","first-page":"182","article-title":"Determination and analysis of polysaccharide content in stem and leaves of different sweet potato varieties","volume":"46","author":"Zhao","year":"2018","journal-title":"J. Shanxi Agric. Sci."},{"key":"ref_37","unstructured":"Wang, W. (2013). Study on the Preparation Process Andquality Standards of Senecio cannabifolius Extract, CCUCM."},{"key":"ref_38","first-page":"149","article-title":"Extraction of chlorogenic acid from folium cortex eucommiae by enzymatic hydrolysis method and determination of its content","volume":"35","author":"Zhang","year":"2016","journal-title":"China Brew."},{"key":"ref_39","first-page":"30","article-title":"Extraction process of chlorogenic acid from wild chrysanthemum flower via supercritical fluid extraction","volume":"33","author":"Li","year":"2012","journal-title":"Food Res. Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"113925","DOI":"10.1016\/j.scienta.2024.113925","article-title":"Polyphenol content, antioxidant capacity, and composition in different varieties of sweet potato (Ipomoea batatas L.) leaves during growth stages","volume":"342","author":"Shi","year":"2025","journal-title":"Sci. Hortic."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/fbe2.70021","article-title":"Emerging techniques for the recovery of bioactive compounds from sweet potato leaves [Ipomoea batatas (L.) Lam] and their functional health benefits","volume":"4","author":"Jahurul","year":"2025","journal-title":"Food Bioeng."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Dewi, N.K.S.M., Ramona, Y., Saraswati, M., Wihandani, D., and Wirasuta, I. (2023). The potential of the flavonoid content of Ipomoea batatas L. as an alternative analog GLP-1 for diabetes type 2 treatment-systematic review. Metabolites, 14.","DOI":"10.20944\/preprints202312.0265.v1"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.biopha.2017.10.064","article-title":"Chlorogenic acid(CGA): Apharmacological review and call for further research","volume":"97","author":"Naveed","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4826","DOI":"10.1039\/D0FO01168A","article-title":"Protection against developing type 2 diabetes by coffee consumption: Assessment of the role of chlorogenic acid and metabolites on glycaemic responses","volume":"11","author":"Gary","year":"2020","journal-title":"Food Funct."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Yan, Y., Li, Q., Shen, L., Guo, K., and Zhou, X. (2022). Chlorogenic acid improves glucose tolerance, lipid metabolism, inflammation and microbiota composition in diabetic db\/db mice. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.1042044"},{"key":"ref_46","first-page":"103","article-title":"Inhibitory effects of chlorogenic acid and isochlorogenic acid from purple sweet potato leaves on \u03b1-glucosidase","volume":"30","author":"Liu","year":"2014","journal-title":"Mod. Food Sci. Tech."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"106772","DOI":"10.1016\/j.jsbmb.2025.106772","article-title":"(-)-Epigallocatechin-3-gallate and chlorogenic acid in combination with vitamin D as a therapeutic approach for letrozole-induced polycystic ovary syndrome (PCOS) rats: Biochemical and hormonal modulation","volume":"252","author":"Siddiqui","year":"2025","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_48","first-page":"172","article-title":"Mechanism of Lonicerae japonicae Flos in the treatment of diabetes based on netwwork pharmacology","volume":"3","author":"Han","year":"2022","journal-title":"J. Shandong First Med. Uni. (Shandong Acad. Med. Sci.)"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4371","DOI":"10.1021\/jf400920x","article-title":"Supplementation of a high-fat diet with chlorogenic acid is associated with insulin resistance and hepatic lipid accumulation in mice","volume":"61","author":"Mubarak","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.1161\/ATVBAHA.107.153668","article-title":"Aortic Msx2-Wnt calcification cascade is regulated by TNF-alpha-dependent signals in diabetic Ldlr-\/- mice","volume":"27","author":"Shao","year":"2007","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_51","first-page":"48","article-title":"Effect of glucose-control and N-Acetylcysteine on PARP and GAPDH activities in diabetic patients with chronic kidney disease in diabetes","volume":"5","author":"Zhu","year":"2013","journal-title":"Chin. J. Diabetes"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.cbi.2010.07.026","article-title":"Antihyperlipidemic effect of chlorogenic acid and tetrahydrocurcumin in rats subjected to diabetogenic agents","volume":"188","author":"Karthikesan","year":"2010","journal-title":"Chem.-Biol. Interact."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/1\/120\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T09:40:11Z","timestamp":1767346811000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/1\/120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,1]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["plants15010120"],"URL":"https:\/\/doi.org\/10.3390\/plants15010120","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,1]]}}}