{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T13:55:59Z","timestamp":1769435759136,"version":"3.49.0"},"reference-count":51,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"crossref","award":["ANPCyT-PICT-2021-I-A-01090"],"award-info":[{"award-number":["ANPCyT-PICT-2021-I-A-01090"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010677","name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","doi-asserted-by":"crossref","award":["I083"],"award-info":[{"award-number":["I083"]}],"id":[{"id":"10.13039\/501100010677","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010677","name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","doi-asserted-by":"crossref","award":["I085"],"award-info":[{"award-number":["I085"]}],"id":[{"id":"10.13039\/501100010677","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010677","name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","doi-asserted-by":"crossref","award":["I086"],"award-info":[{"award-number":["I086"]}],"id":[{"id":"10.13039\/501100010677","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010677","name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","doi-asserted-by":"crossref","award":["I127"],"award-info":[{"award-number":["I127"]}],"id":[{"id":"10.13039\/501100010677","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Potato virus Y (PVY) and potato leafroll virus (PLRV) are the most damaging viruses for potato production worldwide. Mixed infections not only result in additive detrimental effects on plant growth and tuber yield but also complicate the development of durable and broad-spectrum viral resistance. Heterologous protection against PVY can be achieved through the expression of the coat protein (CP) of lettuce mosaic virus (LMV) (CPLMV), conferring resistance via a capsid protein-mediated mechanism. On the other hand, we have previously demonstrated that transgenic lines expressing the PLRV ORF2 (RepPLRV) exhibit resistance to different PLRV isolates. In this study, potato transgenic lines of cv. Kennebec expressing CPLMV and RepPLRV were developed to confer dual virus resistance. Transgenic and non-transgenic control plants were molecularly and phenotypically characterized in greenhouse and field conditions. Across multiple growing seasons, two selected transgenic lines consistently displayed robust resistance to both major viruses, without exhibiting yield penalties or noticeable phenotypic alterations. These results constitute a significant advancement, demonstrating that dual resistance to PVY and PLRV can be achieved while preserving the original agronomic performance of the cultivar. This breakthrough not only contributes to long-term crop productivity but also provides a more sustainable strategy for managing viral diseases in potato production.<\/jats:p>","DOI":"10.3390\/plants15030355","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T14:58:40Z","timestamp":1769180320000},"page":"355","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stable Resistance to Potato Virus Y and Potato Leafroll Virus in Transgenic Potato Plants cv. Kennebec Expressing Viral Genes Under Greenhouse and Field Conditions"],"prefix":"10.3390","volume":"15","author":[{"given":"Mar\u00eda Pilar","family":"Barrios Bar\u00f3n","sequence":"first","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2961-6488","authenticated-orcid":false,"given":"Natalia In\u00e9s","family":"Almasia","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8210-9266","authenticated-orcid":false,"given":"Vanesa","family":"Nahir\u00f1ak","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9021-2175","authenticated-orcid":false,"given":"Diego","family":"Zavallo","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deimer Daniel","family":"Rodriguez Diaz","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9516-5948","authenticated-orcid":false,"given":"Sebasti\u00e1n","family":"Asurmendi","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Fuligna","sequence":"additional","affiliation":[{"name":"EEA-INTA AER La Consulta, Ex Ruta 40 Km 96, La Consulta, San Carlos\u00a05567, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2230-641X","authenticated-orcid":false,"given":"Horacio Esteban","family":"Hopp","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"},{"name":"Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente G\u00fciraldes 2160, Ciudad Aut\u00f3noma de Buenos Aires C1428, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9685-1103","authenticated-orcid":false,"given":"Ana Julia","family":"Dist\u00e9fano","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5804-6197","authenticated-orcid":false,"given":"Cecilia","family":"Vazquez Rovere","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO) UEDD INTA-CONICET, Instituto de Biotecnolog\u00eda, Centro de Investigaciones de Ciencias Veterinarias y Agron\u00f3micas INTA, Hurlingham B1686, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Noureen, A., Khan, M.Z., Amin, I., Zainab, T., and Mansoor, S. 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