{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T03:18:48Z","timestamp":1781925528264,"version":"3.54.5"},"reference-count":37,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Innovation Team for the Soybean Modern Seed Industry in Hebei","award":["21326313D"],"award-info":[{"award-number":["21326313D"]}]},{"name":"Construction of the Soybean Industry Innovation Team under the Hebei Modern Agricultural Industrial Technology System","award":["HBCT2023040203"],"award-info":[{"award-number":["HBCT2023040203"]}]},{"DOI":"10.13039\/501100018522","name":"Jiangsu Collaborative Innovation Center for Modern Crop Production","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100018522","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Core Technological Development for Breeding Program of Jiangsu Province","award":["JBGS-2021-014"],"award-info":[{"award-number":["JBGS-2021-014"]}]},{"name":"R&D Program of Beijing Municipal Education Commission","award":["KM202312448004"],"award-info":[{"award-number":["KM202312448004"]}]},{"name":"the Scientific Research Innovation Team Project of Beijing Vocational College of Agriculture","award":["XY-TD-25-01"],"award-info":[{"award-number":["XY-TD-25-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The soybean mosaic virus (SMV), a significant viral pathogen impacting soybean cultivation, leads to substantial yield losses and diminishes seed quality. In a prior study, we developed a targeted silencing vector using RNA interference (RNAi) technology targeting the CP gene, which codes for the viral coat proteins in the SMV genome. This vector was delivered into soybean plants through Agrobacterium-mediated transformation. In our current research, we utilized ongoing molecular characterization and resistance screening to identify four genetically pure lines that display moderate to high resistance to SMV. Additionally, the transgenic plants exhibited resistance to three other potyviruses: the bean common mosaic virus, the recombinant soybean mosaic virus, and the watermelon mosaic virus. Greenhouse and field trials conducted with these lines demonstrated that RNAi-mediated silencing of the CP gene significantly enhanced disease resistance. It is noteworthy that, in comparison to the receptor plants, the transgenic plants exhibited no significant differences in maturity, plant height, branching number, node number, pod number, or 100-seed weight. These results offer valuable genetic resources and theoretical support for molecular breeding strategies aimed at combating SMV in soybeans, as well as for RNAi-based methods to control plant viral infections.<\/jats:p>","DOI":"10.3390\/plants15030430","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T13:04:46Z","timestamp":1769778286000},"page":"430","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enhanced Soybean Immunity to the Soybean Mosaic Virus Through RNA Interference Targeting the CP Gene"],"prefix":"10.3390","volume":"15","author":[{"given":"Tao","family":"Wang","sequence":"first","affiliation":[{"name":"National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China"},{"name":"Handan Academy of Agricultural Sciences, Handan 056001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4852-8894","authenticated-orcid":false,"given":"Le","family":"Gao","sequence":"additional","affiliation":[{"name":"National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China"},{"name":"Department of Horticulture, Beijing Vocational College of Agriculture, Beijing 102442, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqun","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7850-8750","authenticated-orcid":false,"given":"Rui","family":"Ren","sequence":"additional","affiliation":[{"name":"National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China"},{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhai","sequence":"additional","affiliation":[{"name":"National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Wang","sequence":"additional","affiliation":[{"name":"Handan Academy of Agricultural Sciences, Handan 056001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuming","family":"Xiao","sequence":"additional","affiliation":[{"name":"Handan Academy of Agricultural Sciences, Handan 056001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Yan","sequence":"additional","affiliation":[{"name":"Hebei Laboratory of Crop Genetics and Breeding, National Soybean Improvement Center Shijiazhuang Sub-Center, Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture and Rural Affairs, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotong","family":"Lei","sequence":"additional","affiliation":[{"name":"Handan Academy of Agricultural Sciences, Handan 056001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2718-8774","authenticated-orcid":false,"given":"Tongtong","family":"Jin","sequence":"additional","affiliation":[{"name":"National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China"},{"name":"Xuzhou Institute of Agricultural Sciences of Xu-Huai Region of Jiangsu, Xuzhou 221131, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijian","family":"Zhi","sequence":"additional","affiliation":[{"name":"National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Widyasari, K., Alazem, M., and Kim, K.H. 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